Restriction map for
pF1KB5083
(Linear) (Six-base) MAPSORT of: KIBB5083.seq Check: 912 from: 1 to: 2055
KIBB5083.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 221 320 811 889 2055
Size: 221 99 491 78 1166
Fragments arranged by size:
1166 491 221 99 78
Acc65I G'GTAC_C
Cuts at: 0 724 1550 2055
Size: 724 826 505
Fragments arranged by size:
826 724 505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1792 2055
Size: 767 1025 263
Fragments arranged by size:
1025 767 263
AfeI AGC'GCT
Cuts at: 0 241 2055
Size: 241 1814
AhdI GACnn_n'nnGTC
Cuts at: 0 1026 2055
Size: 1026 1029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 2055
Size: 947 1108
AlwNI CAG_nnn'CTG
Cuts at: 0 63 1105 2055
Size: 63 1042 950
Fragments arranged by size:
1042 950 63
ApaI G_GGCC'C
Cuts at: 0 795 2055
Size: 795 1260
ApoI r'AATT_y
Cuts at: 0 404 1083 1869 2055
Size: 404 679 786 186
Fragments arranged by size:
786 679 404 186
AseI AT'TA_AT
Cuts at: 0 638 2055
Size: 638 1417
AvaI C'yCGr_G
Cuts at: 0 115 192 2055
Size: 115 77 1863
Fragments arranged by size:
1863 115 77
BanI G'GyrC_C
Cuts at: 0 724 1550 1855 2055
Size: 724 826 305 200
Fragments arranged by size:
826 724 305 200
BanII G_rGCy'C
Cuts at: 0 795 1259 2055
Size: 795 464 796
Fragments arranged by size:
796 795 464
BbsI GAAGACnn'nnnn_
Cuts at: 0 1022 1184 1397 1794 2055
Size: 1022 162 213 397 261
Fragments arranged by size:
1022 397 261 213 162
BclI T'GATC_A
Cuts at: 0 1881 2055
Size: 1881 174
BglII A'GATC_T
Cuts at: 0 203 612 1761 2055
Size: 203 409 1149 294
Fragments arranged by size:
1149 409 294 203
BlpI GC'TnA_GC
Cuts at: 0 1489 2055
Size: 1489 566
Bme1580I G_kGCm'C
Cuts at: 0 155 795 2055
Size: 155 640 1260
Fragments arranged by size:
1260 640 155
BmrI ACTGGGnnnn_n'
Cuts at: 0 242 962 2055
Size: 242 720 1093
Fragments arranged by size:
1093 720 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 2055
Size: 1243 812
Bpu10I CC'TnA_GC
Cuts at: 0 1192 1685 2055
Size: 1192 493 370
Fragments arranged by size:
1192 493 370
BsaWI w'CCGG_w
Cuts at: 0 1017 2055
Size: 1017 1038
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1665 1695 2055
Size: 1665 30 360
Fragments arranged by size:
1665 360 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1267 1753 2055
Size: 1267 486 302
Fragments arranged by size:
1267 486 302
BseYI C'CCAG_C
Cuts at: 0 63 261 1074 1231 1668 2055
Size: 63 198 813 157 437 387
Fragments arranged by size:
813 437 387 198 157 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 2055
Size: 448 1607
BsiEI CG_ry'CG
Cuts at: 0 103 2055
Size: 103 1952
BsiHKAI G_wGCw'C
Cuts at: 0 1259 1495 1599 2055
Size: 1259 236 104 456
Fragments arranged by size:
1259 456 236 104
BsmI GAATG_Cn'
Cuts at: 0 1387 1472 2055
Size: 1387 85 583
Fragments arranged by size:
1387 583 85
Bsp1286I G_dGCh'C
Cuts at: 0 155 795 1259 1495 1599 2055
Size: 155 640 464 236 104 456
Fragments arranged by size:
640 464 456 236 155 104
BspEI T'CCGG_A
Cuts at: 0 1017 2055
Size: 1017 1038
BspHI T'CATG_A
Cuts at: 0 588 2055
Size: 588 1467
BsrDI GCAATG_nn'
Cuts at: 0 904 2055
Size: 904 1151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 74 426 1105 1382 1678 2055
Size: 74 352 679 277 296 377
Fragments arranged by size:
679 377 352 296 277 74
BstBI TT'CG_AA
Cuts at: 0 1785 2055
Size: 1785 270
BstEII G'GTnAC_C
Cuts at: 0 1509 2055
Size: 1509 546
BstYI r'GATC_y
Cuts at: 0 203 612 1761 2055
Size: 203 409 1149 294
Fragments arranged by size:
1149 409 294 203
BstZ17I GTA'TAC
Cuts at: 0 812 890 2055
Size: 812 78 1165
Fragments arranged by size:
1165 812 78
Bsu36I CC'TnA_GG
Cuts at: 0 556 2055
Size: 556 1499
BtgI C'CryG_G
Cuts at: 0 647 1400 2055
Size: 647 753 655
Fragments arranged by size:
753 655 647
BtsI GCAGTG_nn'
Cuts at: 0 1383 2055
Size: 1383 672
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1260 1295 2055
Size: 1260 35 760
Fragments arranged by size:
1260 760 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1570 2055
Size: 1570 485
EaeI y'GGCC_r
Cuts at: 0 100 1650 2055
Size: 100 1550 405
Fragments arranged by size:
1550 405 100
EagI C'GGCC_G
Cuts at: 0 100 2055
Size: 100 1955
EcoICRI GAG'CTC
Cuts at: 0 1257 2055
Size: 1257 798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1243 1792 2055
Size: 767 476 549 263
Fragments arranged by size:
767 549 476 263
EcoO109I rG'GnC_Cy
Cuts at: 0 792 830 1830 2055
Size: 792 38 1000 225
Fragments arranged by size:
1000 792 225 38
EcoRI G'AATT_C
Cuts at: 0 404 2055
Size: 404 1651
EcoRV GAT'ATC
Cuts at: 0 1952 2055
Size: 1952 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 1104 1939 2055
Size: 291 813 835 116
Fragments arranged by size:
835 813 291 116
HaeII r_GCGC'y
Cuts at: 0 64 243 2055
Size: 64 179 1812
Fragments arranged by size:
1812 179 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 539 571 1012 1044 1781 1813 1936 1968
Size: 539 32 441 32 737 32 123 32
Cuts at: 1968 2055
Size: 87
Fragments arranged by size:
737 539 441 123 87 32 32 32
32
HincII GTy'rAC
Cuts at: 0 222 530 2055
Size: 222 308 1525
Fragments arranged by size:
1525 308 222
HindIII A'AGCT_T
Cuts at: 0 1189 2055
Size: 1189 866
KpnI G_GTAC'C
Cuts at: 0 728 1554 2055
Size: 728 826 501
Fragments arranged by size:
826 728 501
MscI TGG'CCA
Cuts at: 0 1652 2055
Size: 1652 403
MslI CAynn'nnrTG
Cuts at: 0 46 419 773 1503 2055
Size: 46 373 354 730 552
Fragments arranged by size:
730 552 373 354 46
MspA1I CmG'CkG
Cuts at: 0 1102 1364 1488 2055
Size: 1102 262 124 567
Fragments arranged by size:
1102 567 262 124
NcoI C'CATG_G
Cuts at: 0 647 1400 2055
Size: 647 753 655
Fragments arranged by size:
753 655 647
NotI GC'GGCC_GC
Cuts at: 0 100 2055
Size: 100 1955
NspI r_CATG'y
Cuts at: 0 424 1458 2037 2055
Size: 424 1034 579 18
Fragments arranged by size:
1034 579 424 18
PasI CC'CwG_GG
Cuts at: 0 795 2055
Size: 795 1260
PshAI GACnn'nnGTC
Cuts at: 0 671 2055
Size: 671 1384
PspOMI G'GGCC_C
Cuts at: 0 791 2055
Size: 791 1264
PstI C_TGCA'G
Cuts at: 0 487 2055
Size: 487 1568
PvuII CAG'CTG
Cuts at: 0 1102 1364 1488 2055
Size: 1102 262 124 567
Fragments arranged by size:
1102 567 262 124
SacI G_AGCT'C
Cuts at: 0 1259 2055
Size: 1259 796
SalI G'TCGA_C
Cuts at: 0 220 2055
Size: 220 1835
SfcI C'TryA_G
Cuts at: 0 483 1365 1902 2055
Size: 483 882 537 153
Fragments arranged by size:
882 537 483 153
StyI C'CwwG_G
Cuts at: 0 647 695 1400 2055
Size: 647 48 705 655
Fragments arranged by size:
705 655 647 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 2055
Size: 661 1394
XmnI GAAnn'nnTTC
Cuts at: 0 1922 2055
Size: 1922 133
Enzymes that do cut:
AccI Acc65I AcuI AfeI AhdI AlfI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI BclI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BsrDI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtsI CspCI DrdI EaeI EagI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KpnI
MscI MslI MspA1I NcoI NotI NspI PasI PshAI
PspOMI PstI PvuII SacI SalI SfcI StyI TaqII
XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AleI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BfrBI BglI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsiWI BsmBI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstXI BtgZI ClaI DraI
DraIII EarI EciI EcoNI FseI FspI FspAI HpaI
KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI