Restriction map for
pF1KB5080
(Linear) (Six-base) MAPSORT of: KIBB5080.seq Check: 2605 from: 1 to: 2175
KIBB5080.orf
With 182 enzymes: SgfI *
May 21, 2013 16:22 ..
AccI GT'mk_AC
Cuts at: 0 749 1645 2175
Size: 749 896 530
Fragments arranged by size:
896 749 530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 908 1567 2175
Size: 592 316 659 608
Fragments arranged by size:
659 608 592 316
AgeI A'CCGG_T
Cuts at: 0 50 2175
Size: 50 2125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 1154 2175
Size: 208 946 1021
Fragments arranged by size:
1021 946 208
AlwNI CAG_nnn'CTG
Cuts at: 0 570 874 958 1057 2175
Size: 570 304 84 99 1118
Fragments arranged by size:
1118 570 304 99 84
ApaLI G'TGCA_C
Cuts at: 0 1207 1450 2175
Size: 1207 243 725
Fragments arranged by size:
1207 725 243
ApoI r'AATT_y
Cuts at: 0 252 409 667 2175
Size: 252 157 258 1508
Fragments arranged by size:
1508 258 252 157
AvaI C'yCGr_G
Cuts at: 0 66 1223 2175
Size: 66 1157 952
Fragments arranged by size:
1157 952 66
BanII G_rGCy'C
Cuts at: 0 1924 2175
Size: 1924 251
BbsI GAAGACnn'nnnn_
Cuts at: 0 1635 2175
Size: 1635 540
BclI T'GATC_A
Cuts at: 0 786 1046 1593 2175
Size: 786 260 547 582
Fragments arranged by size:
786 582 547 260
BfrBI ATG'CAT
Cuts at: 0 261 2175
Size: 261 1914
Bme1580I G_kGCm'C
Cuts at: 0 1211 1454 2175
Size: 1211 243 721
Fragments arranged by size:
1211 721 243
BmrI ACTGGGnnnn_n'
Cuts at: 0 220 2175
Size: 220 1955
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2035 2175
Size: 2035 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1274 2012 2175
Size: 539 735 738 163
Fragments arranged by size:
738 735 539 163
Bpu10I CC'TnA_GC
Cuts at: 0 1947 2108 2175
Size: 1947 161 67
Fragments arranged by size:
1947 161 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 2175
Size: 1534 641
BsaI GGTCTCn'nnnn_
Cuts at: 0 1546 2175
Size: 1546 629
BsaWI w'CCGG_w
Cuts at: 0 50 2175
Size: 50 2125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 512 542 1259 1289 1985 2015 2175
Size: 512 30 717 30 696 30 160
Fragments arranged by size:
717 696 512 160 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 185 446 644 874 1211 2158 2175
Size: 185 261 198 230 337 947 17
Fragments arranged by size:
947 337 261 230 198 185 17
BseYI C'CCAG_C
Cuts at: 0 308 867 903 1441 2175
Size: 308 559 36 538 734
Fragments arranged by size:
734 559 538 308 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 853 1767 2175
Size: 347 506 914 408
Fragments arranged by size:
914 506 408 347
BsiHKAI G_wGCw'C
Cuts at: 0 493 1211 1454 1924 2109 2175
Size: 493 718 243 470 185 66
Fragments arranged by size:
718 493 470 243 185 66
BsmBI CGTCTCn'nnnn_
Cuts at: 0 67 1262 2175
Size: 67 1195 913
Fragments arranged by size:
1195 913 67
Bsp1286I G_dGCh'C
Cuts at: 0 493 1211 1454 1924 2109 2175
Size: 493 718 243 470 185 66
Fragments arranged by size:
718 493 470 243 185 66
BspHI T'CATG_A
Cuts at: 0 605 2175
Size: 605 1570
BsrBI CCG'CTC
Cuts at: 0 301 2175
Size: 301 1874
BsrDI GCAATG_nn'
Cuts at: 0 158 804 2175
Size: 158 646 1371
Fragments arranged by size:
1371 646 158
BsrFI r'CCGG_y
Cuts at: 0 50 2175
Size: 50 2125
BsrGI T'GTAC_A
Cuts at: 0 216 2175
Size: 216 1959
BstBI TT'CG_AA
Cuts at: 0 1145 2175
Size: 1145 1030
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 781 1756 2043 2175
Size: 210 571 975 287 132
Fragments arranged by size:
975 571 287 210 132
BstYI r'GATC_y
Cuts at: 0 1167 2175
Size: 1167 1008
BstZ17I GTA'TAC
Cuts at: 0 1646 2175
Size: 1646 529
BtgI C'CryG_G
Cuts at: 0 792 2175
Size: 792 1383
BtsI GCAGTG_nn'
Cuts at: 0 1626 2175
Size: 1626 549
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1227 1262 1380 1415 2175
Size: 1227 35 118 35 760
Fragments arranged by size:
1227 760 118 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1894 2175
Size: 1894 281
EaeI y'GGCC_r
Cuts at: 0 347 1422 2175
Size: 347 1075 753
Fragments arranged by size:
1075 753 347
EarI CTCTTCn'nnn_
Cuts at: 0 1586 2175
Size: 1586 589
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 2175
Size: 55 2120
EcoICRI GAG'CTC
Cuts at: 0 1922 2175
Size: 1922 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 592 908 1274 1567 2012 2175
Size: 539 53 316 366 293 445 163
Fragments arranged by size:
539 445 366 316 293 163 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1193 2175
Size: 1193 982
EcoO109I rG'GnC_Cy
Cuts at: 0 153 451 864 2175
Size: 153 298 413 1311
Fragments arranged by size:
1311 413 298 153
FspI TGC'GCA
Cuts at: 0 196 2175
Size: 196 1979
FspAI rTGC'GCAy
Cuts at: 0 196 2175
Size: 196 1979
HaeII r_GCGC'y
Cuts at: 0 874 2175
Size: 874 1301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 828 860 1585 1617 1622 1654
Size: 541 32 255 32 725 32 5 32
Cuts at: 1654 2137 2169 2175
Size: 483 32 6
Fragments arranged by size:
725 541 483 255 32 32 32 32
32 6 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1362 1477 2175
Size: 927 435 115 698
Fragments arranged by size:
927 698 435 115
MscI TGG'CCA
Cuts at: 0 349 1424 2175
Size: 349 1075 751
Fragments arranged by size:
1075 751 349
MslI CAynn'nnrTG
Cuts at: 0 96 343 797 1003 1189 2026 2041 2175
Size: 96 247 454 206 186 837 15 134
Fragments arranged by size:
837 454 247 206 186 134 96 15
NcoI C'CATG_G
Cuts at: 0 792 2175
Size: 792 1383
NsiI A_TGCA'T
Cuts at: 0 263 2175
Size: 263 1912
NspI r_CATG'y
Cuts at: 0 265 766 1728 2175
Size: 265 501 962 447
Fragments arranged by size:
962 501 447 265
PasI CC'CwG_GG
Cuts at: 0 1536 2175
Size: 1536 639
PflMI CCAn_nnn'nTGG
Cuts at: 0 874 2071 2175
Size: 874 1197 104
Fragments arranged by size:
1197 874 104
PfoI T'CCnGG_A
Cuts at: 0 378 1838 2175
Size: 378 1460 337
Fragments arranged by size:
1460 378 337
PpuMI rG'GwC_Cy
Cuts at: 0 153 2175
Size: 153 2022
PspXI vC'TCGA_Gb
Cuts at: 0 1223 2175
Size: 1223 952
PstI C_TGCA'G
Cuts at: 0 187 918 1133 1509 2175
Size: 187 731 215 376 666
Fragments arranged by size:
731 666 376 215 187
SacI G_AGCT'C
Cuts at: 0 1924 2175
Size: 1924 251
SbfI CC_TGCA'GG
Cuts at: 0 187 2175
Size: 187 1988
SfcI C'TryA_G
Cuts at: 0 183 825 914 1129 1505 2094 2175
Size: 183 642 89 215 376 589 81
Fragments arranged by size:
642 589 376 215 183 89 81
SmlI C'TyrA_G
Cuts at: 0 1223 1549 2175
Size: 1223 326 626
Fragments arranged by size:
1223 626 326
SphI G_CATG'C
Cuts at: 0 1728 2175
Size: 1728 447
SspI AAT'ATT
Cuts at: 0 1377 2175
Size: 1377 798
StyI C'CwwG_G
Cuts at: 0 149 792 1755 1865 2175
Size: 149 643 963 110 310
Fragments arranged by size:
963 643 310 149 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 84 202 1442 1582 2175
Size: 84 118 1240 140 593
Fragments arranged by size:
1240 593 140 118 84
TatI w'GTAC_w
Cuts at: 0 119 216 806 1370 2175
Size: 119 97 590 564 805
Fragments arranged by size:
805 590 564 119 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 356 388 1985 2017 2175
Size: 356 32 1597 32 158
Fragments arranged by size:
1597 356 158 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 2175
Size: 504 1671
XhoI C'TCGA_G
Cuts at: 0 1223 2175
Size: 1223 952
XmnI GAAnn'nnTTC
Cuts at: 0 712 2089 2175
Size: 712 1377 86
Fragments arranged by size:
1377 712 86
Enzymes that do cut:
AccI AcuI AgeI AlfI AlwNI ApaLI ApoI AvaI
BanII BbsI BclI BfrBI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI
BstBI BstXI BstYI BstZ17I BtgI BtsI CspCI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
FspAI HaeII Hin4I MmeI MscI MslI NcoI NsiI
NspI PasI PflMI PfoI PpuMI PspXI PstI SacI
SbfI SfcI SmlI SphI SspI StyI TaqII TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BglI BglII
BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BspEI BspMI BssHII BssSI BstAPI BstEII Bsu36I
BtgZI ClaI DraI DraIII EagI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI Tth111I XbaI XmaI ZraI