Restriction map for
pF1KB5060
(Linear) (Six-base) MAPSORT of: KIBB5060.seq Check: 390 from: 1 to: 1755
KIBB5060.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 0 1601 1755
Size: 1601 154
AccI GT'mk_AC
Cuts at: 0 1442 1755
Size: 1442 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 985 1755
Size: 346 639 770
Fragments arranged by size:
770 639 346
AhdI GACnn_n'nnGTC
Cuts at: 0 309 1755
Size: 309 1446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 751 783 1755
Size: 751 32 972
Fragments arranged by size:
972 751 32
AlwNI CAG_nnn'CTG
Cuts at: 0 219 552 846 1140 1170 1265 1755
Size: 219 333 294 294 30 95 490
Fragments arranged by size:
490 333 294 294 219 95 30
ApaI G_GGCC'C
Cuts at: 0 192 1755
Size: 192 1563
ApaLI G'TGCA_C
Cuts at: 0 276 1271 1755
Size: 276 995 484
Fragments arranged by size:
995 484 276
ApoI r'AATT_y
Cuts at: 0 1144 1755
Size: 1144 611
BanI G'GyrC_C
Cuts at: 0 88 942 1755
Size: 88 854 813
Fragments arranged by size:
854 813 88
BanII G_rGCy'C
Cuts at: 0 192 416 1067 1502 1586 1755
Size: 192 224 651 435 84 169
Fragments arranged by size:
651 435 224 192 169 84
BbeI G_GCGC'C
Cuts at: 0 92 1755
Size: 92 1663
BbsI GAAGACnn'nnnn_
Cuts at: 0 1132 1755
Size: 1132 623
BciVI GTATCCnnnnn_n'
Cuts at: 0 1197 1755
Size: 1197 558
BglI GCCn_nnn'nGGC
Cuts at: 0 134 214 298 452 703 1755
Size: 134 80 84 154 251 1052
Fragments arranged by size:
1052 251 154 134 84 80
BglII A'GATC_T
Cuts at: 0 113 434 1755
Size: 113 321 1321
Fragments arranged by size:
1321 321 113
Bme1580I G_kGCm'C
Cuts at: 0 139 192 280 608 947 1160 1275 1641
Size: 139 53 88 328 339 213 115 366
Cuts at: 1641 1694 1755
Size: 53 61
Fragments arranged by size:
366 339 328 213 139 115 88 61
53 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 884 1628 1755
Size: 884 744 127
Fragments arranged by size:
884 744 127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1017 1755
Size: 1017 738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 559 844 1002 1119 1260 1287 1755
Size: 390 169 285 158 117 141 27 468
Fragments arranged by size:
468 390 285 169 158 141 117 27
BsaBI GATnn'nnATC
Cuts at: 0 652 1755
Size: 652 1103
BsaHI Gr'CG_yC
Cuts at: 0 89 494 815 1598 1755
Size: 89 405 321 783 157
Fragments arranged by size:
783 405 321 157 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 622 652 1465 1495 1755
Size: 622 30 813 30 260
Fragments arranged by size:
813 622 260 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 1024 1075 1479 1659 1731 1755
Size: 739 285 51 404 180 72 24
Fragments arranged by size:
739 404 285 180 72 51 24
BseYI C'CCAG_C
Cuts at: 0 946 986 1399 1755
Size: 946 40 413 356
Fragments arranged by size:
946 413 356 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 502 1755
Size: 351 151 1253
Fragments arranged by size:
1253 351 151
BsiHKAI G_wGCw'C
Cuts at: 0 280 388 416 1067 1275 1502 1666 1755
Size: 280 108 28 651 208 227 164 89
Fragments arranged by size:
651 280 227 208 164 108 89 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 344 1755
Size: 344 1411
Bsp1286I G_dGCh'C
Cuts at: 0 139 192 280 388 416 608 947 1067
Size: 139 53 88 108 28 192 339 120
Cuts at: 1067 1160 1275 1502 1586 1641 1666 1694 1755
Size: 93 115 227 84 55 25 28 61
Fragments arranged by size:
339 227 192 139 120 115 108 93
88 84 61 55 53 28 28 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 914 1755
Size: 914 841
BsrDI GCAATG_nn'
Cuts at: 0 579 1544 1755
Size: 579 965 211
Fragments arranged by size:
965 579 211
BssSI C'ACGA_G
Cuts at: 0 343 1105 1755
Size: 343 762 650
Fragments arranged by size:
762 650 343
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 588 954 1641 1755
Size: 261 327 366 687 114
Fragments arranged by size:
687 366 327 261 114
BstYI r'GATC_y
Cuts at: 0 113 434 1034 1755
Size: 113 321 600 721
Fragments arranged by size:
721 600 321 113
Bsu36I CC'TnA_GG
Cuts at: 0 1058 1743 1755
Size: 1058 685 12
Fragments arranged by size:
1058 685 12
BtgI C'CryG_G
Cuts at: 0 608 971 1755
Size: 608 363 784
Fragments arranged by size:
784 608 363
BtsI GCAGTG_nn'
Cuts at: 0 806 1755
Size: 806 949
DraIII CAC_nnn'GTG
Cuts at: 0 143 1155 1755
Size: 143 1012 600
Fragments arranged by size:
1012 600 143
EaeI y'GGCC_r
Cuts at: 0 77 299 429 510 803 1755
Size: 77 222 130 81 293 952
Fragments arranged by size:
952 293 222 130 81 77
EarI CTCTTCn'nnn_
Cuts at: 0 22 322 1414 1591 1654 1755
Size: 22 300 1092 177 63 101
Fragments arranged by size:
1092 300 177 101 63 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1708 1755
Size: 1708 47
EcoICRI GAG'CTC
Cuts at: 0 414 1065 1500 1755
Size: 414 651 435 255
Fragments arranged by size:
651 435 414 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 390 559 844 985 1002 1119 1260
Size: 346 44 169 285 141 17 117 141
Cuts at: 1260 1287 1755
Size: 27 468
Fragments arranged by size:
468 346 285 169 141 141 117 44
27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 754 964 1198 1411 1755
Size: 556 198 210 234 213 344
Fragments arranged by size:
556 344 234 213 210 198
EcoO109I rG'GnC_Cy
Cuts at: 0 159 189 250 423 549 618 759 1110
Size: 159 30 61 173 126 69 141 351
Cuts at: 1110 1137 1309 1755
Size: 27 172 446
Fragments arranged by size:
446 351 173 172 159 141 126 69
61 30 27
EcoRI G'AATT_C
Cuts at: 0 1144 1755
Size: 1144 611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1056 1755
Size: 1056 699
FspI TGC'GCA
Cuts at: 0 729 1755
Size: 729 1026
HaeII r_GCGC'y
Cuts at: 0 92 1755
Size: 92 1663
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1755
Size: 295 32 1428
Fragments arranged by size:
1428 295 32
KasI G'GCGC_C
Cuts at: 0 88 1755
Size: 88 1667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1755
Size: 936 819
MscI TGG'CCA
Cuts at: 0 431 512 805 1755
Size: 431 81 293 950
Fragments arranged by size:
950 431 293 81
MslI CAynn'nnrTG
Cuts at: 0 472 586 1755
Size: 472 114 1169
Fragments arranged by size:
1169 472 114
MspA1I CmG'CkG
Cuts at: 0 450 714 1755
Size: 450 264 1041
Fragments arranged by size:
1041 450 264
NarI GG'CG_CC
Cuts at: 0 89 1755
Size: 89 1666
NcoI C'CATG_G
Cuts at: 0 608 1755
Size: 608 1147
NspI r_CATG'y
Cuts at: 0 72 1755
Size: 72 1683
PasI CC'CwG_GG
Cuts at: 0 192 315 498 1755
Size: 192 123 183 1257
Fragments arranged by size:
1257 192 183 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 396 687 1170 1755
Size: 219 177 291 483 585
Fragments arranged by size:
585 483 291 219 177
PfoI T'CCnGG_A
Cuts at: 0 1264 1412 1755
Size: 1264 148 343
Fragments arranged by size:
1264 343 148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1063 1095 1099 1131 1755
Size: 1063 32 4 32 624
Fragments arranged by size:
1063 624 32 32 4
PpuMI rG'GwC_Cy
Cuts at: 0 159 618 759 1755
Size: 159 459 141 996
Fragments arranged by size:
996 459 159 141
PspOMI G'GGCC_C
Cuts at: 0 188 1755
Size: 188 1567
PstI C_TGCA'G
Cuts at: 0 340 719 1079 1136 1755
Size: 340 379 360 57 619
Fragments arranged by size:
619 379 360 340 57
PvuII CAG'CTG
Cuts at: 0 714 1755
Size: 714 1041
SacI G_AGCT'C
Cuts at: 0 416 1067 1502 1755
Size: 416 651 435 253
Fragments arranged by size:
651 435 416 253
SapI GCTCTTCn'nnn_
Cuts at: 0 22 322 1591 1654 1755
Size: 22 300 1269 63 101
Fragments arranged by size:
1269 300 101 63 22
SfcI C'TryA_G
Cuts at: 0 120 336 715 838 1075 1132 1356 1755
Size: 120 216 379 123 237 57 224 399
Fragments arranged by size:
399 379 237 224 216 123 120 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 298 1755
Size: 298 1457
SfoI GGC'GCC
Cuts at: 0 90 1755
Size: 90 1665
SspI AAT'ATT
Cuts at: 0 1324 1755
Size: 1324 431
StuI AGG'CCT
Cuts at: 0 292 865 883 1187 1755
Size: 292 573 18 304 568
Fragments arranged by size:
573 568 304 292 18
StyI C'CwwG_G
Cuts at: 0 48 426 608 1755
Size: 48 378 182 1147
Fragments arranged by size:
1147 378 182 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 226 403 1755
Size: 12 214 177 1352
Fragments arranged by size:
1352 214 177 12
TatI w'GTAC_w
Cuts at: 0 1094 1755
Size: 1094 661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 583 615 1755
Size: 583 32 1140
Fragments arranged by size:
1140 583 32
Tth111I GACn'n_nGTC
Cuts at: 0 268 1755
Size: 268 1487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 933 1755
Size: 216 717 822
Fragments arranged by size:
822 717 216
ZraI GAC'GTC
Cuts at: 0 1599 1755
Size: 1599 156
Enzymes that do cut:
AatII AccI AcuI AhdI AloI AlwNI ApaI ApaLI
ApoI BanI BanII BbeI BbsI BciVI BglI BglII
Bme1580I BmrI BplI BpmI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BssSI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI
NcoI NspI PasI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SapI SfcI SfiI SfoI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbvCI BcgI BclI BfrBI BlpI BmgBI BmtI Bpu10I
BpuEI BsaI BsaAI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
XbaI XhoI XmaI XmnI