Restriction map for
pF1KB5057
(Linear) (Six-base) MAPSORT of: KIBB5057.seq Check: 3759 from: 1 to: 1599
KIBB5057.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 75 1599
Size: 75 1524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1341 1598 1599
Size: 1108 233 257 1
Fragments arranged by size:
1108 257 233 1
AflIII A'CryG_T
Cuts at: 0 779 1599
Size: 779 820
AleI CACnn'nnGTG
Cuts at: 0 172 235 851 1599
Size: 172 63 616 748
Fragments arranged by size:
748 616 172 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1402 1599
Size: 1402 197
AlwNI CAG_nnn'CTG
Cuts at: 0 570 1599
Size: 570 1029
ApaI G_GGCC'C
Cuts at: 0 744 1599
Size: 744 855
ApaLI G'TGCA_C
Cuts at: 0 613 1599
Size: 613 986
ApoI r'AATT_y
Cuts at: 0 446 1599
Size: 446 1153
BanI G'GyrC_C
Cuts at: 0 727 853 1285 1599
Size: 727 126 432 314
Fragments arranged by size:
727 432 314 126
BanII G_rGCy'C
Cuts at: 0 744 1343 1599
Size: 744 599 256
Fragments arranged by size:
744 599 256
BbeI G_GCGC'C
Cuts at: 0 731 1289 1599
Size: 731 558 310
Fragments arranged by size:
731 558 310
BbsI GAAGACnn'nnnn_
Cuts at: 0 514 1347 1599
Size: 514 833 252
Fragments arranged by size:
833 514 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 627 661 1599
Size: 627 34 938
Fragments arranged by size:
938 627 34
BclI T'GATC_A
Cuts at: 0 338 1599
Size: 338 1261
BglII A'GATC_T
Cuts at: 0 62 584 1599
Size: 62 522 1015
Fragments arranged by size:
1015 522 62
BlpI GC'TnA_GC
Cuts at: 0 1178 1599
Size: 1178 421
Bme1580I G_kGCm'C
Cuts at: 0 617 705 744 1599
Size: 617 88 39 855
Fragments arranged by size:
855 617 88 39
BmgBI CAC'GTC
Cuts at: 0 780 1521 1599
Size: 780 741 78
Fragments arranged by size:
780 741 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 1599
Size: 663 936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 274 598 1599
Size: 193 81 324 1001
Fragments arranged by size:
1001 324 193 81
Bpu10I CC'TnA_GC
Cuts at: 0 106 1108 1599
Size: 106 1002 491
Fragments arranged by size:
1002 491 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 549 1504 1599
Size: 549 955 95
Fragments arranged by size:
955 549 95
BsaHI Gr'CG_yC
Cuts at: 0 728 1286 1599
Size: 728 558 313
Fragments arranged by size:
728 558 313
BsaWI w'CCGG_w
Cuts at: 0 1403 1599
Size: 1403 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 701 731 1599
Size: 701 30 868
Fragments arranged by size:
868 701 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 595 611 1524 1599
Size: 130 465 16 913 75
Fragments arranged by size:
913 465 130 75 16
BseYI C'CCAG_C
Cuts at: 0 1039 1096 1546 1599
Size: 1039 57 450 53
Fragments arranged by size:
1039 450 57 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1217 1599
Size: 755 462 382
Fragments arranged by size:
755 462 382
BsiHKAI G_wGCw'C
Cuts at: 0 29 617 1599
Size: 29 588 982
Fragments arranged by size:
982 588 29
BsmI GAATG_Cn'
Cuts at: 0 954 1098 1264 1599
Size: 954 144 166 335
Fragments arranged by size:
954 335 166 144
Bsp1286I G_dGCh'C
Cuts at: 0 29 617 705 744 1343 1599
Size: 29 588 88 39 599 256
Fragments arranged by size:
599 588 256 88 39 29
BspEI T'CCGG_A
Cuts at: 0 1403 1599
Size: 1403 196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 75 1394 1599
Size: 75 1319 205
Fragments arranged by size:
1319 205 75
BsrBI CCG'CTC
Cuts at: 0 402 1599
Size: 402 1197
BsrDI GCAATG_nn'
Cuts at: 0 1433 1599
Size: 1433 166
BstEII G'GTnAC_C
Cuts at: 0 174 1599
Size: 174 1425
BstYI r'GATC_y
Cuts at: 0 62 249 584 1599
Size: 62 187 335 1015
Fragments arranged by size:
1015 335 187 62
BtgI C'CryG_G
Cuts at: 0 705 799 1271 1599
Size: 705 94 472 328
Fragments arranged by size:
705 472 328 94
EaeI y'GGCC_r
Cuts at: 0 350 753 1268 1599
Size: 350 403 515 331
Fragments arranged by size:
515 403 350 331
EarI CTCTTCn'nnn_
Cuts at: 0 785 1599
Size: 785 814
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1171 1599
Size: 1171 428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 274 598 1108 1341 1598 1599
Size: 193 81 324 510 233 257 1
Fragments arranged by size:
510 324 257 233 193 81 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1138 1456 1599
Size: 1138 318 143
Fragments arranged by size:
1138 318 143
EcoO109I rG'GnC_Cy
Cuts at: 0 741 957 1043 1383 1599
Size: 741 216 86 340 216
Fragments arranged by size:
741 340 216 216 86
EcoRI G'AATT_C
Cuts at: 0 446 1599
Size: 446 1153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 294 1599
Size: 294 1305
HaeII r_GCGC'y
Cuts at: 0 731 1289 1599
Size: 731 558 310
Fragments arranged by size:
731 558 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 576 608 1599
Size: 576 32 991
Fragments arranged by size:
991 576 32
KasI G'GCGC_C
Cuts at: 0 727 1285 1599
Size: 727 558 314
Fragments arranged by size:
727 558 314
MscI TGG'CCA
Cuts at: 0 352 755 1599
Size: 352 403 844
Fragments arranged by size:
844 403 352
MslI CAynn'nnrTG
Cuts at: 0 172 235 610 827 851 1063 1599
Size: 172 63 375 217 24 212 536
Fragments arranged by size:
536 375 217 212 172 63 24
MspA1I CmG'CkG
Cuts at: 0 181 199 570 839 881 1599
Size: 181 18 371 269 42 718
Fragments arranged by size:
718 371 269 181 42 18
NarI GG'CG_CC
Cuts at: 0 728 1286 1599
Size: 728 558 313
Fragments arranged by size:
728 558 313
NspI r_CATG'y
Cuts at: 0 1573 1599
Size: 1573 26
PasI CC'CwG_GG
Cuts at: 0 441 1599
Size: 441 1158
PflMI CCAn_nnn'nTGG
Cuts at: 0 216 992 1302 1599
Size: 216 776 310 297
Fragments arranged by size:
776 310 297 216
PpuMI rG'GwC_Cy
Cuts at: 0 1043 1383 1599
Size: 1043 340 216
Fragments arranged by size:
1043 340 216
PspOMI G'GGCC_C
Cuts at: 0 740 1599
Size: 740 859
PstI C_TGCA'G
Cuts at: 0 86 137 749 1337 1599
Size: 86 51 612 588 262
Fragments arranged by size:
612 588 262 86 51
PvuII CAG'CTG
Cuts at: 0 199 570 839 1599
Size: 199 371 269 760
Fragments arranged by size:
760 371 269 199
SanDI GG'GwC_CC
Cuts at: 0 1043 1599
Size: 1043 556
SapI GCTCTTCn'nnn_
Cuts at: 0 785 1599
Size: 785 814
SfcI C'TryA_G
Cuts at: 0 82 133 512 745 1333 1599
Size: 82 51 379 233 588 266
Fragments arranged by size:
588 379 266 233 82 51
SfoI GGC'GCC
Cuts at: 0 729 1287 1599
Size: 729 558 312
Fragments arranged by size:
729 558 312
StuI AGG'CCT
Cuts at: 0 149 1599
Size: 149 1450
StyI C'CwwG_G
Cuts at: 0 170 812 863 991 1314 1599
Size: 170 642 51 128 323 285
Fragments arranged by size:
642 323 285 170 128 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 841 1599
Size: 841 758
TatI w'GTAC_w
Cuts at: 0 1329 1599
Size: 1329 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 701 733 1599
Size: 701 32 866
Fragments arranged by size:
866 701 32
XmnI GAAnn'nnTTC
Cuts at: 0 409 1599
Size: 409 1190
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI BanI BanII BbeI BbsI BcgI BclI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BstEII BstYI BtgI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I KasI MscI MslI MspA1I NarI NspI PasI
PflMI PpuMI PspOMI PstI PvuII SanDI SapI SfcI
SfoI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbvCI BciVI BfrBI BglI BmtI BplI BpuEI BsaAI
BsaBI BsiEI BsiWI BsmBI BspHI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI