Restriction map for
pF1KB5046
(Linear) (Six-base) MAPSORT of: KIBB5046.seq Check: 2112 from: 1 to: 1188
KIBB5046.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 523 1188
Size: 184 339 665
Fragments arranged by size:
665 339 184
AlwNI CAG_nnn'CTG
Cuts at: 0 207 626 696 1077 1188
Size: 207 419 70 381 111
Fragments arranged by size:
419 381 207 111 70
ApaI G_GGCC'C
Cuts at: 0 375 735 972 1188
Size: 375 360 237 216
Fragments arranged by size:
375 360 237 216
AvaI C'yCGr_G
Cuts at: 0 415 1188
Size: 415 773
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 153 186 1188
Size: 153 33 1002
Fragments arranged by size:
1002 153 33
BanI G'GyrC_C
Cuts at: 0 1117 1188
Size: 1117 71
BanII G_rGCy'C
Cuts at: 0 375 433 639 735 890 972 1188
Size: 375 58 206 96 155 82 216
Fragments arranged by size:
375 216 206 155 96 82 58
BciVI GTATCCnnnnn_n'
Cuts at: 0 102 1188
Size: 102 1086
BglI GCCn_nnn'nGGC
Cuts at: 0 739 1188
Size: 739 449
Bme1580I G_kGCm'C
Cuts at: 0 375 735 972 1122 1188
Size: 375 360 237 150 66
Fragments arranged by size:
375 360 237 150 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 655 1188
Size: 655 533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 417 673 838 1063 1188
Size: 244 173 256 165 225 125
Fragments arranged by size:
256 244 225 173 165 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 748 1015 1188
Size: 123 625 267 173
Fragments arranged by size:
625 267 173 123
BsaHI Gr'CG_yC
Cuts at: 0 746 1127 1188
Size: 746 381 61
Fragments arranged by size:
746 381 61
BsaWI w'CCGG_w
Cuts at: 0 601 1188
Size: 601 587
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 647 1018 1188
Size: 499 148 371 170
Fragments arranged by size:
499 371 170 148
BseYI C'CCAG_C
Cuts at: 0 834 855 1188
Size: 834 21 333
Fragments arranged by size:
834 333 21
BsiHKAI G_wGCw'C
Cuts at: 0 890 1188
Size: 890 298
BsmI GAATG_Cn'
Cuts at: 0 921 1188
Size: 921 267
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1134 1188
Size: 1134 54
Bsp1286I G_dGCh'C
Cuts at: 0 375 433 639 735 890 972 1122 1188
Size: 375 58 206 96 155 82 150 66
Fragments arranged by size:
375 206 155 150 96 82 66 58
BspEI T'CCGG_A
Cuts at: 0 601 1188
Size: 601 587
BsrDI GCAATG_nn'
Cuts at: 0 141 1188
Size: 141 1047
BssSI C'ACGA_G
Cuts at: 0 874 1188
Size: 874 314
BstEII G'GTnAC_C
Cuts at: 0 960 1188
Size: 960 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 136 816 1152 1188
Size: 136 680 336 36
Fragments arranged by size:
680 336 136 36
BstYI r'GATC_y
Cuts at: 0 488 692 1188
Size: 488 204 496
Fragments arranged by size:
496 488 204
BtgI C'CryG_G
Cuts at: 0 62 972 1188
Size: 62 910 216
Fragments arranged by size:
910 216 62
DrdI GACnn_nn'nnGTC
Cuts at: 0 304 616 1162 1188
Size: 304 312 546 26
Fragments arranged by size:
546 312 304 26
EaeI y'GGCC_r
Cuts at: 0 191 1188
Size: 191 997
EarI CTCTTCn'nnn_
Cuts at: 0 878 1188
Size: 878 310
EcoICRI GAG'CTC
Cuts at: 0 888 1188
Size: 888 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 244 417 523 673 838 1063 1188
Size: 184 60 173 106 150 165 225 125
Fragments arranged by size:
225 184 173 165 150 125 106 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 672 1188
Size: 672 516
EcoO109I rG'GnC_Cy
Cuts at: 0 371 372 731 732 968 1074 1188
Size: 371 1 359 1 236 106 114
Fragments arranged by size:
371 359 236 114 106 1 1
EcoRV GAT'ATC
Cuts at: 0 390 1188
Size: 390 798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 582 1188
Size: 61 521 606
Fragments arranged by size:
606 521 61
HaeII r_GCGC'y
Cuts at: 0 318 440 1188
Size: 318 122 748
Fragments arranged by size:
748 318 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 796 828 1188
Size: 796 32 360
Fragments arranged by size:
796 360 32
HincII GTy'rAC
Cuts at: 0 522 1188
Size: 522 666
HindIII A'AGCT_T
Cuts at: 0 216 1188
Size: 216 972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1188
Size: 383 805
MscI TGG'CCA
Cuts at: 0 193 1188
Size: 193 995
MslI CAynn'nnrTG
Cuts at: 0 134 895 914 1188
Size: 134 761 19 274
Fragments arranged by size:
761 274 134 19
MspA1I CmG'CkG
Cuts at: 0 501 974 1085 1188
Size: 501 473 111 103
Fragments arranged by size:
501 473 111 103
NspI r_CATG'y
Cuts at: 0 61 1188
Size: 61 1127
PspOMI G'GGCC_C
Cuts at: 0 371 731 968 1188
Size: 371 360 237 220
Fragments arranged by size:
371 360 237 220
PvuII CAG'CTG
Cuts at: 0 1085 1188
Size: 1085 103
SacI G_AGCT'C
Cuts at: 0 890 1188
Size: 890 298
SacII CC_GC'GG
Cuts at: 0 975 1188
Size: 975 213
SapI GCTCTTCn'nnn_
Cuts at: 0 878 1188
Size: 878 310
SmaI CCC'GGG
Cuts at: 0 417 1188
Size: 417 771
SmlI C'TyrA_G
Cuts at: 0 102 763 1030 1188
Size: 102 661 267 158
Fragments arranged by size:
661 267 158 102
StyI C'CwwG_G
Cuts at: 0 533 570 1188
Size: 533 37 618
Fragments arranged by size:
618 533 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 1188
Size: 261 927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 540 735 1188
Size: 540 195 453
Fragments arranged by size:
540 453 195
XmaI C'CCGG_G
Cuts at: 0 415 1188
Size: 415 773
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BaeI BanI BanII BciVI
BglI Bme1580I BplI BpmI BpuEI BsaHI BsaWI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrDI BssSI
BstEII BstXI BstYI BtgI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII MmeI MscI MslI MspA1I NspI PspOMI PvuII
SacI SacII SapI SmaI SmlI StyI TaqII XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaI BsaAI BsaBI BsaXI BsgI BsiEI BsiWI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI
EciI EcoRI FseI FspI FspAI HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI