(Linear) (Six-base) MAPSORT of: KIBB5013.seq  Check: 5767  from: 1  to: 609



KIBB5013.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AatII G_ACGT'C

Cuts at:      0      64     609 
   Size:         64     545
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     157     160     270     609 
   Size:         78      79       3     110     339

  Fragments arranged by size: 

                 339     110      79      78       3
 
AfeI AGC'GCT

Cuts at:      0     205     609 
   Size:        205     404
 
AgeI A'CCGG_T

Cuts at:      0     419     609 
   Size:        419     190
 
ApoI r'AATT_y

Cuts at:      0     525     609 
   Size:        525      84
 
AseI AT'TA_AT

Cuts at:      0     485     609 
   Size:        485     124
 
BanI G'GyrC_C

Cuts at:      0     351     609 
   Size:        351     258
 
Bme1580I G_kGCm'C

Cuts at:      0     356     609 
   Size:        356     253
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     576     609 
   Size:        130     446      33

  Fragments arranged by size: 

                 446     130      33
 
BsaHI Gr'CG_yC

Cuts at:      0      44      61     609 
   Size:         44      17     548

  Fragments arranged by size: 

                 548      44      17
 
BsaWI w'CCGG_w

Cuts at:      0     419     609 
   Size:        419     190
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     335     365     609 
   Size:        335      30     244

  Fragments arranged by size: 

                 335     244      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     609 
   Size:         91     518
 
Bsp1286I G_dGCh'C

Cuts at:      0     356     609 
   Size:        356     253
 
BsrFI r'CCGG_y

Cuts at:      0     348     419     609 
   Size:        348      71     190

  Fragments arranged by size: 

                 348     190      71
 
BtgI C'CryG_G

Cuts at:      0     113     609 
   Size:        113     496
 
BtsI GCAGTG_nn'

Cuts at:      0      56     404     609 
   Size:         56     348     205

  Fragments arranged by size: 

                 348     205      56
 
EaeI y'GGCC_r

Cuts at:      0     368     609 
   Size:        368     241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     130     157     160     270     576     609 
   Size:         78      52      27       3     110     306      33

  Fragments arranged by size: 

                 306     110      78      52      33      27       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     312     609 
   Size:        312     297
 
EcoRV GAT'ATC

Cuts at:      0     540     609 
   Size:        540      69
 
HaeII r_GCGC'y

Cuts at:      0     207     609 
   Size:        207     402
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     529     561     609 
   Size:        529      32      48

  Fragments arranged by size: 

                 529      48      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     609 
   Size:        430     179
 
MscI TGG'CCA

Cuts at:      0     370     609 
   Size:        370     239
 
MslI CAynn'nnrTG

Cuts at:      0     214     609 
   Size:        214     395
 
MspA1I CmG'CkG

Cuts at:      0     180     596     609 
   Size:        180     416      13

  Fragments arranged by size: 

                 416     180      13
 
PpuMI rG'GwC_Cy

Cuts at:      0     312     609 
   Size:        312     297
 
PvuII CAG'CTG

Cuts at:      0     180     596     609 
   Size:        180     416      13

  Fragments arranged by size: 

                 416     180      13
 
SspI AAT'ATT

Cuts at:      0     483     609 
   Size:        483     126
 
StyI C'CwwG_G

Cuts at:      0     391     558     609 
   Size:        391     167      51

  Fragments arranged by size: 

                 391     167      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     571     609 
   Size:        571      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      94     126     335     367     609 
   Size:         94      32     209      32     242

  Fragments arranged by size: 

                 242     209      94      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     609 
   Size:        120     489
 
XmnI GAAnn'nnTTC

Cuts at:      0     439     609 
   Size:        439     170
 
ZraI GAC'GTC

Cuts at:      0      62     609 
   Size:         62     547
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AgeI     ApoI     AseI     BanI Bme1580I
     BpmI    BsaHI    BsaWI    BsaXI     BsgI Bsp1286I    BsrFI     BtgI
     BtsI     EaeI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I    PpuMI    PvuII     SspI     StyI    TaqII
     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI