Restriction map for
pF1KB5011
(Linear) (Six-base) MAPSORT of: KIBB5011.seq Check: 1739 from: 1 to: 1251
KIBB5011.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 912 1251
Size: 912 339
AlwNI CAG_nnn'CTG
Cuts at: 0 180 363 774 1200 1251
Size: 180 183 411 426 51
Fragments arranged by size:
426 411 183 180 51
BanII G_rGCy'C
Cuts at: 0 234 1251
Size: 234 1017
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 671 1232 1251
Size: 584 87 561 19
Fragments arranged by size:
584 561 87 19
BglI GCCn_nnn'nGGC
Cuts at: 0 898 1251
Size: 898 353
BlpI GC'TnA_GC
Cuts at: 0 106 740 1251
Size: 106 634 511
Fragments arranged by size:
634 511 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1251
Size: 385 866
Bpu10I CC'TnA_GC
Cuts at: 0 1186 1251
Size: 1186 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 680 1251
Size: 409 271 571
Fragments arranged by size:
571 409 271
BsaWI w'CCGG_w
Cuts at: 0 56 1097 1251
Size: 56 1041 154
Fragments arranged by size:
1041 154 56
BseYI C'CCAG_C
Cuts at: 0 526 1251
Size: 526 725
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 787 1251
Size: 784 3 464
Fragments arranged by size:
784 464 3
BsiHKAI G_wGCw'C
Cuts at: 0 234 1251
Size: 234 1017
Bsp1286I G_dGCh'C
Cuts at: 0 234 1251
Size: 234 1017
BspEI T'CCGG_A
Cuts at: 0 56 1097 1251
Size: 56 1041 154
Fragments arranged by size:
1041 154 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 912 1251
Size: 701 211 339
Fragments arranged by size:
701 339 211
BsrDI GCAATG_nn'
Cuts at: 0 887 1251
Size: 887 364
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 618 1251
Size: 267 351 633
Fragments arranged by size:
633 351 267
BtsI GCAGTG_nn'
Cuts at: 0 209 775 796 944 968 1251
Size: 209 566 21 148 24 283
Fragments arranged by size:
566 283 209 148 24 21
EaeI y'GGCC_r
Cuts at: 0 21 761 884 1251
Size: 21 740 123 367
Fragments arranged by size:
740 367 123 21
EarI CTCTTCn'nnn_
Cuts at: 0 26 675 1022 1251
Size: 26 649 347 229
Fragments arranged by size:
649 347 229 26
EcoICRI GAG'CTC
Cuts at: 0 232 1251
Size: 232 1019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1251
Size: 385 866
EcoO109I rG'GnC_Cy
Cuts at: 0 279 1251
Size: 279 972
FspI TGC'GCA
Cuts at: 0 196 1251
Size: 196 1055
HaeII r_GCGC'y
Cuts at: 0 181 1251
Size: 181 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1092 1124 1251
Size: 85 32 975 32 127
Fragments arranged by size:
975 127 85 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 1251
Size: 516 735
MscI TGG'CCA
Cuts at: 0 23 886 1251
Size: 23 863 365
Fragments arranged by size:
863 365 23
MslI CAynn'nnrTG
Cuts at: 0 265 457 988 1251
Size: 265 192 531 263
Fragments arranged by size:
531 265 263 192
MspA1I CmG'CkG
Cuts at: 0 166 285 997 1125 1251
Size: 166 119 712 128 126
Fragments arranged by size:
712 166 128 126 119
NspI r_CATG'y
Cuts at: 0 484 1251
Size: 484 767
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 363 1251
Size: 180 183 888
Fragments arranged by size:
888 183 180
PpuMI rG'GwC_Cy
Cuts at: 0 279 1251
Size: 279 972
PsiI TTA'TAA
Cuts at: 0 787 911 1251
Size: 787 124 340
Fragments arranged by size:
787 340 124
PstI C_TGCA'G
Cuts at: 0 635 1251
Size: 635 616
PvuII CAG'CTG
Cuts at: 0 166 997 1125 1251
Size: 166 831 128 126
Fragments arranged by size:
831 166 128 126
SacI G_AGCT'C
Cuts at: 0 234 1251
Size: 234 1017
ScaI AGT'ACT
Cuts at: 0 511 1251
Size: 511 740
SexAI A'CCwGG_T
Cuts at: 0 44 1251
Size: 44 1207
SfcI C'TryA_G
Cuts at: 0 631 1251
Size: 631 620
SmlI C'TyrA_G
Cuts at: 0 388 695 1251
Size: 388 307 556
Fragments arranged by size:
556 388 307
SspI AAT'ATT
Cuts at: 0 753 1251
Size: 753 498
StyI C'CwwG_G
Cuts at: 0 18 1251
Size: 18 1233
TatI w'GTAC_w
Cuts at: 0 509 1251
Size: 509 742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 181 523 894 1251
Size: 181 342 371 357
Fragments arranged by size:
371 357 342 181
Enzymes that do cut:
AarI AlwNI BanII BbsI BglI BlpI BpmI Bpu10I
BpuEI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BsrDI BstXI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I MmeI MscI MslI MspA1I NspI
PflMI PpuMI PsiI PstI PvuII SacI ScaI SexAI
SfcI SmlI SspI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I
BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI