Restriction map for
pF1KB5004
(Linear) (Six-base) MAPSORT of: KIBB5004.seq Check: 6170 from: 1 to: 1146
KIBB5004.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 0 329 1146
Size: 329 817
AccI GT'mk_AC
Cuts at: 0 77 1146
Size: 77 1069
Acc65I G'GTAC_C
Cuts at: 0 216 922 1146
Size: 216 706 224
Fragments arranged by size:
706 224 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1146
Size: 1033 113
AflIII A'CryG_T
Cuts at: 0 572 1146
Size: 572 574
AhdI GACnn_n'nnGTC
Cuts at: 0 122 1146
Size: 122 1024
AlwNI CAG_nnn'CTG
Cuts at: 0 61 373 1146
Size: 61 312 773
Fragments arranged by size:
773 312 61
ApaI G_GGCC'C
Cuts at: 0 35 381 1146
Size: 35 346 765
Fragments arranged by size:
765 346 35
ApaLI G'TGCA_C
Cuts at: 0 16 136 1146
Size: 16 120 1010
Fragments arranged by size:
1010 120 16
ApoI r'AATT_y
Cuts at: 0 958 1146
Size: 958 188
AvaI C'yCGr_G
Cuts at: 0 353 380 1146
Size: 353 27 766
Fragments arranged by size:
766 353 27
AvrII C'CTAG_G
Cuts at: 0 405 1146
Size: 405 741
BanI G'GyrC_C
Cuts at: 0 216 713 875 922 1146
Size: 216 497 162 47 224
Fragments arranged by size:
497 224 216 162 47
BanII G_rGCy'C
Cuts at: 0 35 381 1146
Size: 35 346 765
Fragments arranged by size:
765 346 35
BbeI G_GCGC'C
Cuts at: 0 717 879 1146
Size: 717 162 267
Fragments arranged by size:
717 267 162
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 1021 1146
Size: 113 908 125
Fragments arranged by size:
908 125 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 681 715 1146
Size: 681 34 431
Fragments arranged by size:
681 431 34
BglI GCCn_nnn'nGGC
Cuts at: 0 711 1146
Size: 711 435
Bme1580I G_kGCm'C
Cuts at: 0 20 35 140 381 1146
Size: 20 15 105 241 765
Fragments arranged by size:
765 241 105 20 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 555 680 1146
Size: 43 512 125 466
Fragments arranged by size:
512 466 125 43
BsaHI Gr'CG_yC
Cuts at: 0 326 714 876 1146
Size: 326 388 162 270
Fragments arranged by size:
388 326 270 162
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1125 1146
Size: 1125 21
BseYI C'CCAG_C
Cuts at: 0 561 1146
Size: 561 585
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1146
Size: 512 634
BsiEI CG_ry'CG
Cuts at: 0 600 1146
Size: 600 546
BsiHKAI G_wGCw'C
Cuts at: 0 20 140 314 1146
Size: 20 120 174 832
Fragments arranged by size:
832 174 120 20
BsmI GAATG_Cn'
Cuts at: 0 644 1098 1146
Size: 644 454 48
Fragments arranged by size:
644 454 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1137 1146
Size: 1137 9
Bsp1286I G_dGCh'C
Cuts at: 0 20 35 140 314 381 1146
Size: 20 15 105 174 67 765
Fragments arranged by size:
765 174 105 67 20 15
BspHI T'CATG_A
Cuts at: 0 1071 1146
Size: 1071 75
BsrDI GCAATG_nn'
Cuts at: 0 312 1146
Size: 312 834
BsrFI r'CCGG_y
Cuts at: 0 785 872 1146
Size: 785 87 274
Fragments arranged by size:
785 274 87
BsrGI T'GTAC_A
Cuts at: 0 419 511 752 1146
Size: 419 92 241 394
Fragments arranged by size:
419 394 241 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 1146
Size: 561 585
BstZ17I GTA'TAC
Cuts at: 0 78 1146
Size: 78 1068
Bsu36I CC'TnA_GG
Cuts at: 0 707 938 1146
Size: 707 231 208
Fragments arranged by size:
707 231 208
BtgI C'CryG_G
Cuts at: 0 198 1140 1146
Size: 198 942 6
Fragments arranged by size:
942 198 6
BtsI GCAGTG_nn'
Cuts at: 0 62 118 158 1146
Size: 62 56 40 988
Fragments arranged by size:
988 62 56 40
DrdI GACnn_nn'nnGTC
Cuts at: 0 262 907 1146
Size: 262 645 239
Fragments arranged by size:
645 262 239
EaeI y'GGCC_r
Cuts at: 0 1031 1146
Size: 1031 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 297 463 1146
Size: 297 166 683
Fragments arranged by size:
683 297 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 555 680 1033 1146
Size: 43 512 125 353 113
Fragments arranged by size:
512 353 125 113 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 941 1146
Size: 941 205
EcoO109I rG'GnC_Cy
Cuts at: 0 32 299 377 1146
Size: 32 267 78 769
Fragments arranged by size:
769 267 78 32
EcoRV GAT'ATC
Cuts at: 0 1077 1146
Size: 1077 69
HaeII r_GCGC'y
Cuts at: 0 717 879 1146
Size: 717 162 267
Fragments arranged by size:
717 267 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 486 518 532 564 1146
Size: 108 32 346 32 14 32 582
Fragments arranged by size:
582 346 108 32 32 32 14
KasI G'GCGC_C
Cuts at: 0 713 875 1146
Size: 713 162 271
Fragments arranged by size:
713 271 162
KpnI G_GTAC'C
Cuts at: 0 220 926 1146
Size: 220 706 220
Fragments arranged by size:
706 220 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 644 1146
Size: 145 499 502
Fragments arranged by size:
502 499 145
MslI CAynn'nnrTG
Cuts at: 0 575 794 1146
Size: 575 219 352
Fragments arranged by size:
575 352 219
NaeI GCC'GGC
Cuts at: 0 874 1146
Size: 874 272
NarI GG'CG_CC
Cuts at: 0 714 876 1146
Size: 714 162 270
Fragments arranged by size:
714 270 162
NcoI C'CATG_G
Cuts at: 0 1140 1146
Size: 1140 6
NgoMIV G'CCGG_C
Cuts at: 0 872 1146
Size: 872 274
NspI r_CATG'y
Cuts at: 0 228 576 1146
Size: 228 348 570
Fragments arranged by size:
570 348 228
PciI A'CATG_T
Cuts at: 0 572 1146
Size: 572 574
PflMI CCAn_nnn'nTGG
Cuts at: 0 497 1146
Size: 497 649
PsiI TTA'TAA
Cuts at: 0 770 1146
Size: 770 376
PspOMI G'GGCC_C
Cuts at: 0 31 377 1146
Size: 31 346 769
Fragments arranged by size:
769 346 31
PstI C_TGCA'G
Cuts at: 0 57 1146
Size: 57 1089
SfcI C'TryA_G
Cuts at: 0 53 1146
Size: 53 1093
SfoI GGC'GCC
Cuts at: 0 715 877 1146
Size: 715 162 269
Fragments arranged by size:
715 269 162
SgrAI Cr'CCGG_yG
Cuts at: 0 872 1146
Size: 872 274
SmaI CCC'GGG
Cuts at: 0 382 1146
Size: 382 764
StuI AGG'CCT
Cuts at: 0 947 1146
Size: 947 199
StyI C'CwwG_G
Cuts at: 0 405 1140 1146
Size: 405 735 6
Fragments arranged by size:
735 405 6
TatI w'GTAC_w
Cuts at: 0 419 511 752 1146
Size: 419 92 241 394
Fragments arranged by size:
419 394 241 92
Tth111I GACn'n_nGTC
Cuts at: 0 262 1146
Size: 262 884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 730 1146
Size: 730 416
XmaI C'CCGG_G
Cuts at: 0 380 1146
Size: 380 766
ZraI GAC'GTC
Cuts at: 0 327 1146
Size: 327 819
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AhdI AlwNI ApaI
ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI
BcgI BglI Bme1580I BpmI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI
BsrGI BstXI BstZ17I Bsu36I BtgI BtsI DrdI EaeI
EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI
KpnI MmeI MslI NaeI NarI NcoI NgoMIV NspI
PciI PflMI PsiI PspOMI PstI SfcI SfoI SgrAI
SmaI StuI StyI TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AleI AlfI AloI
AscI AseI AsiSI BaeI BamHI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BspEI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgZI ClaI CspCI DraI DraIII EagI EarI EcoICRI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MscI MspA1I NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI XbaI XhoI XmnI