Restriction map for
pF1KB4977
(Linear) (Six-base) MAPSORT of: KIBB4977.seq Check: 5692 from: 1 to: 1200
KIBB4977.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 0 19 1200
Size: 19 1181
AleI CACnn'nnGTG
Cuts at: 0 673 1200
Size: 673 527
AlwNI CAG_nnn'CTG
Cuts at: 0 66 1200
Size: 66 1134
ApoI r'AATT_y
Cuts at: 0 589 1200
Size: 589 611
BlpI GC'TnA_GC
Cuts at: 0 67 1200
Size: 67 1133
Bme1580I G_kGCm'C
Cuts at: 0 704 1200
Size: 704 496
BmrI ACTGGGnnnn_n'
Cuts at: 0 1028 1200
Size: 1028 172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 497 1200
Size: 497 703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1200
Size: 122 1078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1200
Size: 247 953
BsaAI yAC'GTr
Cuts at: 0 414 1200
Size: 414 786
BsaBI GATnn'nnATC
Cuts at: 0 608 731 839 1200
Size: 608 123 108 361
Fragments arranged by size:
608 361 123 108
BsaWI w'CCGG_w
Cuts at: 0 395 1200
Size: 395 805
BsmI GAATG_Cn'
Cuts at: 0 819 1200
Size: 819 381
BsmBI CGTCTCn'nnnn_
Cuts at: 0 98 1200
Size: 98 1102
Bsp1286I G_dGCh'C
Cuts at: 0 704 1200
Size: 704 496
BsrFI r'CCGG_y
Cuts at: 0 27 1200
Size: 27 1173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1125 1200
Size: 1125 75
BstYI r'GATC_y
Cuts at: 0 324 499 603 1182 1200
Size: 324 175 104 579 18
Fragments arranged by size:
579 324 175 104 18
BtgI C'CryG_G
Cuts at: 0 564 1200
Size: 564 636
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 951 1200
Size: 951 249
BtsI GCAGTG_nn'
Cuts at: 0 78 547 1200
Size: 78 469 653
Fragments arranged by size:
653 469 78
EaeI y'GGCC_r
Cuts at: 0 50 561 1200
Size: 50 511 639
Fragments arranged by size:
639 511 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1200
Size: 122 1078
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 654 686 1200
Size: 510 32 112 32 514
Fragments arranged by size:
514 510 112 32 32
HincII GTy'rAC
Cuts at: 0 20 1200
Size: 20 1180
MscI TGG'CCA
Cuts at: 0 563 1200
Size: 563 637
MslI CAynn'nnrTG
Cuts at: 0 569 673 707 1200
Size: 569 104 34 493
Fragments arranged by size:
569 493 104 34
NaeI GCC'GGC
Cuts at: 0 29 1200
Size: 29 1171
NcoI C'CATG_G
Cuts at: 0 564 1200
Size: 564 636
NgoMIV G'CCGG_C
Cuts at: 0 27 1200
Size: 27 1173
NspI r_CATG'y
Cuts at: 0 45 1200
Size: 45 1155
PflMI CCAn_nnn'nTGG
Cuts at: 0 289 1200
Size: 289 911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 698 730 1200
Size: 698 32 470
Fragments arranged by size:
698 470 32
PsiI TTA'TAA
Cuts at: 0 839 1200
Size: 839 361
SalI G'TCGA_C
Cuts at: 0 18 1200
Size: 18 1182
SfcI C'TryA_G
Cuts at: 0 344 491 1200
Size: 344 147 709
Fragments arranged by size:
709 344 147
SgrAI Cr'CCGG_yG
Cuts at: 0 27 1200
Size: 27 1173
SmlI C'TyrA_G
Cuts at: 0 262 1200
Size: 262 938
SspI AAT'ATT
Cuts at: 0 238 683 1200
Size: 238 445 517
Fragments arranged by size:
517 445 238
StyI C'CwwG_G
Cuts at: 0 564 1200
Size: 564 636
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 730 1200
Size: 730 470
Tth111I GACn'n_nGTC
Cuts at: 0 121 1200
Size: 121 1079
XbaI T'CTAG_A
Cuts at: 0 502 1200
Size: 502 698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 286 1200
Size: 286 914
Enzymes that do cut:
AccI AleI AlwNI ApoI BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaAI BsaBI BsaWI BsmI BsmBI Bsp1286I
BsrFI BstAPI BstYI BtgI BtgZI BtsI EaeI Eco57MI
Hin4I HincII MscI MslI NaeI NcoI NgoMIV NspI
PflMI PpiI PsiI SalI SfcI SgrAI SmlI SspI
StyI TaqII Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmtI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI XhoI XmaI XmnI ZraI