(Linear) (Six-base) MAPSORT of: KIBB4961.seq  Check: 8723  from: 1  to: 654



KIBB4961.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     193     654 
   Size:        167      26     461

  Fragments arranged by size: 

                 461     167      26
 
AflIII A'CryG_T

Cuts at:      0     147     654 
   Size:        147     507
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     374     654 
   Size:        374     280
 
AlwNI CAG_nnn'CTG

Cuts at:      0     335     496     654 
   Size:        335     161     158

  Fragments arranged by size: 

                 335     161     158
 
ApoI r'AATT_y

Cuts at:      0      97     109     603     654 
   Size:         97      12     494      51

  Fragments arranged by size: 

                 494      97      51      12
 
AseI AT'TA_AT

Cuts at:      0     289     388     654 
   Size:        289      99     266

  Fragments arranged by size: 

                 289     266      99
 
AvaI C'yCGr_G

Cuts at:      0      43     443     654 
   Size:         43     400     211

  Fragments arranged by size: 

                 400     211      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      70     577     654 
   Size:         70     507      77

  Fragments arranged by size: 

                 507      77      70
 
BclI T'GATC_A

Cuts at:      0      26     654 
   Size:         26     628
 
BsaHI Gr'CG_yC

Cuts at:      0     272     654 
   Size:        272     382
 
BsaWI w'CCGG_w

Cuts at:      0     242     654 
   Size:        242     412
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509     654 
   Size:        509     145
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     263     654 
   Size:        263     391
 
BspEI T'CCGG_A

Cuts at:      0     242     654 
   Size:        242     412
 
BspHI T'CATG_A

Cuts at:      0     542     654 
   Size:        542     112
 
BsrBI CCG'CTC

Cuts at:      0     237     654 
   Size:        237     417
 
BstYI r'GATC_y

Cuts at:      0     178     654 
   Size:        178     476
 
BtgI C'CryG_G

Cuts at:      0     275     654 
   Size:        275     379
 
BtsI GCAGTG_nn'

Cuts at:      0     221     254     654 
   Size:        221      33     400

  Fragments arranged by size: 

                 400     221      33
 
DraI TTT'AAA

Cuts at:      0     198     654 
   Size:        198     456
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     193     654 
   Size:        167      26     461

  Fragments arranged by size: 

                 461     167      26
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     223     654 
   Size:        223     431
 
HincII GTy'rAC

Cuts at:      0     310     654 
   Size:        310     344
 
NcoI C'CATG_G

Cuts at:      0     275     654 
   Size:        275     379
 
PsiI TTA'TAA

Cuts at:      0     158     654 
   Size:        158     496
 
SfcI C'TryA_G

Cuts at:      0     119     518     654 
   Size:        119     399     136

  Fragments arranged by size: 

                 399     136     119
 
StyI C'CwwG_G

Cuts at:      0     275     654 
   Size:        275     379
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19     186     654 
   Size:         19     167     468

  Fragments arranged by size: 

                 468     167      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     491     523     654 
   Size:        491      32     131

  Fragments arranged by size: 

                 491     131      32
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApoI     AseI     AvaI     BbsI
     BclI    BsaHI    BsaWI     BsgI    BsmBI    BspEI    BspHI    BsrBI
    BstYI     BtgI     BtsI     DraI  Eco57MI     FalI   HincII     NcoI
     PsiI     SfcI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI