Restriction map for
pF1KB4953
(Linear) (Six-base) MAPSORT of: KIBB4953.seq Check: 1051 from: 1 to: 3447
KIBB4953.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 0 1711 3447
Size: 1711 1736
AccI GT'mk_AC
Cuts at: 0 803 3447
Size: 803 2644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1891 2321 2827 3419 3447
Size: 1891 430 506 592 28
Fragments arranged by size:
1891 592 506 430 28
AflII C'TTAA_G
Cuts at: 0 853 3447
Size: 853 2594
AflIII A'CryG_T
Cuts at: 0 2711 3447
Size: 2711 736
AleI CACnn'nnGTG
Cuts at: 0 1423 2396 2914 3447
Size: 1423 973 518 533
Fragments arranged by size:
1423 973 533 518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1270 2042 3447
Size: 1270 772 1405
Fragments arranged by size:
1405 1270 772
AlwNI CAG_nnn'CTG
Cuts at: 0 1159 3213 3447
Size: 1159 2054 234
Fragments arranged by size:
2054 1159 234
ApaLI G'TGCA_C
Cuts at: 0 1269 3447
Size: 1269 2178
ApoI r'AATT_y
Cuts at: 0 142 834 890 1026 1099 1390 1509 2478
Size: 142 692 56 136 73 291 119 969
Cuts at: 2478 3447
Size: 969
Fragments arranged by size:
969 969 692 291 142 136 119 73
56
BamHI G'GATC_C
Cuts at: 0 2000 3447
Size: 2000 1447
BanI G'GyrC_C
Cuts at: 0 721 2497 3447
Size: 721 1776 950
Fragments arranged by size:
1776 950 721
BanII G_rGCy'C
Cuts at: 0 530 765 946 3447
Size: 530 235 181 2501
Fragments arranged by size:
2501 530 235 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 64 85 3447
Size: 64 21 3362
Fragments arranged by size:
3362 64 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1646 1680 3447
Size: 1646 34 1767
Fragments arranged by size:
1767 1646 34
BglII A'GATC_T
Cuts at: 0 2573 3447
Size: 2573 874
BlpI GC'TnA_GC
Cuts at: 0 945 3447
Size: 945 2502
Bme1580I G_kGCm'C
Cuts at: 0 1273 3447
Size: 1273 2174
BmrI ACTGGGnnnn_n'
Cuts at: 0 450 742 1567 2902 3447
Size: 450 292 825 1335 545
Fragments arranged by size:
1335 825 545 450 292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 51 99 3447
Size: 51 48 3348
Fragments arranged by size:
3348 51 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 3119 3235 3447
Size: 1454 1665 116 212
Fragments arranged by size:
1665 1454 212 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3212 3268 3353 3447
Size: 3212 56 85 94
Fragments arranged by size:
3212 94 85 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 2641 3447
Size: 2641 806
BsaAI yAC'GTr
Cuts at: 0 3309 3447
Size: 3309 138
BsaHI Gr'CG_yC
Cuts at: 0 1708 3447
Size: 1708 1739
BsaWI w'CCGG_w
Cuts at: 0 2156 3447
Size: 2156 1291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 452 482 1472 1502 2503 2533 3230 3260
Size: 452 30 990 30 1001 30 697 30
Cuts at: 3260 3447
Size: 187
Fragments arranged by size:
1001 990 697 452 187 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 773 2276 3447
Size: 773 1503 1171
Fragments arranged by size:
1503 1171 773
BseYI C'CCAG_C
Cuts at: 0 131 3447
Size: 131 3316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 1873 3447
Size: 1358 515 1574
Fragments arranged by size:
1574 1358 515
BsiHKAI G_wGCw'C
Cuts at: 0 765 1273 2561 3447
Size: 765 508 1288 886
Fragments arranged by size:
1288 886 765 508
BsmI GAATG_Cn'
Cuts at: 0 966 2754 3447
Size: 966 1788 693
Fragments arranged by size:
1788 966 693
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1699 3447
Size: 1699 1748
Bsp1286I G_dGCh'C
Cuts at: 0 530 765 946 1273 2561 3447
Size: 530 235 181 327 1288 886
Fragments arranged by size:
1288 886 530 327 235 181
BspEI T'CCGG_A
Cuts at: 0 2156 3447
Size: 2156 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 504 3447
Size: 504 2943
BsrBI CCG'CTC
Cuts at: 0 35 3323 3447
Size: 35 3288 124
Fragments arranged by size:
3288 124 35
BsrDI GCAATG_nn'
Cuts at: 0 1254 1339 2421 2666 3447
Size: 1254 85 1082 245 781
Fragments arranged by size:
1254 1082 781 245 85
BsrGI T'GTAC_A
Cuts at: 0 885 2906 3158 3447
Size: 885 2021 252 289
Fragments arranged by size:
2021 885 289 252
BssHII G'CGCG_C
Cuts at: 0 39 3447
Size: 39 3408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3054 3447
Size: 3054 393
BstYI r'GATC_y
Cuts at: 0 29 1128 2000 2573 2609 3447
Size: 29 1099 872 573 36 838
Fragments arranged by size:
1099 872 838 573 36 29
BtgI C'CryG_G
Cuts at: 0 3351 3447
Size: 3351 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2982 3447
Size: 2982 465
BtsI GCAGTG_nn'
Cuts at: 0 164 253 392 1157 1430 3447
Size: 164 89 139 765 273 2017
Fragments arranged by size:
2017 765 273 164 139 89
DraI TTT'AAA
Cuts at: 0 2792 3186 3447
Size: 2792 394 261
Fragments arranged by size:
2792 394 261
EaeI y'GGCC_r
Cuts at: 0 1200 3447
Size: 1200 2247
EarI CTCTTCn'nnn_
Cuts at: 0 216 1871 2294 2806 3447
Size: 216 1655 423 512 641
Fragments arranged by size:
1655 641 512 423 216
EcoICRI GAG'CTC
Cuts at: 0 763 3447
Size: 763 2684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 1891 2321 2827 3119 3235 3419 3447
Size: 1454 437 430 506 292 116 184 28
Fragments arranged by size:
1454 506 437 430 292 184 116 28
EcoRI G'AATT_C
Cuts at: 0 834 3447
Size: 834 2613
EcoRV GAT'ATC
Cuts at: 0 615 3447
Size: 615 2832
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2116 2547 3309 3447
Size: 2116 431 762 138
Fragments arranged by size:
2116 762 431 138
HaeII r_GCGC'y
Cuts at: 0 1010 3447
Size: 1010 2437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1440 1472 1504 2597 2629 3208 3240 3447
Size: 1440 32 32 1093 32 579 32 207
Fragments arranged by size:
1440 1093 579 207 32 32 32 32
HincII GTy'rAC
Cuts at: 0 627 3447
Size: 627 2820
MfeI C'AATT_G
Cuts at: 0 1504 3447
Size: 1504 1943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 438 581 675 1393 3447
Size: 5 433 143 94 718 2054
Fragments arranged by size:
2054 718 433 143 94 5
MscI TGG'CCA
Cuts at: 0 1202 3447
Size: 1202 2245
MslI CAynn'nnrTG
Cuts at: 0 1423 2074 2396 2914 3036 3052 3130 3356
Size: 1423 651 322 518 122 16 78 226
Cuts at: 3356 3447
Size: 91
Fragments arranged by size:
1423 651 518 322 226 122 91 78
16
MspA1I CmG'CkG
Cuts at: 0 135 3447
Size: 135 3312
NcoI C'CATG_G
Cuts at: 0 3351 3447
Size: 3351 96
NdeI CA'TA_TG
Cuts at: 0 3414 3447
Size: 3414 33
NspI r_CATG'y
Cuts at: 0 1140 1247 2715 3447
Size: 1140 107 1468 732
Fragments arranged by size:
1468 1140 732 107
PciI A'CATG_T
Cuts at: 0 2711 3447
Size: 2711 736
PfoI T'CCnGG_A
Cuts at: 0 2237 3447
Size: 2237 1210
PmlI CAC'GTG
Cuts at: 0 3309 3447
Size: 3309 138
PsiI TTA'TAA
Cuts at: 0 472 2086 2182 3447
Size: 472 1614 96 1265
Fragments arranged by size:
1614 1265 472 96
PstI C_TGCA'G
Cuts at: 0 263 2429 3447
Size: 263 2166 1018
Fragments arranged by size:
2166 1018 263
PvuII CAG'CTG
Cuts at: 0 135 3447
Size: 135 3312
SacI G_AGCT'C
Cuts at: 0 765 3447
Size: 765 2682
SapI GCTCTTCn'nnn_
Cuts at: 0 216 3447
Size: 216 3231
SbfI CC_TGCA'GG
Cuts at: 0 2429 3447
Size: 2429 1018
SfcI C'TryA_G
Cuts at: 0 259 1754 2425 3447
Size: 259 1495 671 1022
Fragments arranged by size:
1495 1022 671 259
SmlI C'TyrA_G
Cuts at: 0 853 3227 3283 3368 3447
Size: 853 2374 56 85 79
Fragments arranged by size:
2374 853 85 79 56
SspI AAT'ATT
Cuts at: 0 2470 2684 3447
Size: 2470 214 763
Fragments arranged by size:
2470 763 214
StuI AGG'CCT
Cuts at: 0 2423 3447
Size: 2423 1024
StyI C'CwwG_G
Cuts at: 0 2142 2768 3351 3447
Size: 2142 626 583 96
Fragments arranged by size:
2142 626 583 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2997 3280 3447
Size: 2997 283 167
Fragments arranged by size:
2997 283 167
TatI w'GTAC_w
Cuts at: 0 507 885 2134 2906 3158 3246 3447
Size: 507 378 1249 772 252 88 201
Fragments arranged by size:
1249 772 507 378 252 201 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 177 209 2888 2920 3447
Size: 177 32 2679 32 527
Fragments arranged by size:
2679 527 177 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 21 3447
Size: 21 3426
XmnI GAAnn'nnTTC
Cuts at: 0 966 3387 3447
Size: 966 2421 60
Fragments arranged by size:
2421 966 60
ZraI GAC'GTC
Cuts at: 0 1709 3447
Size: 1709 1738
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AleI AlfI AlwNI
ApaLI ApoI BamHI BanI BanII BbsI BcgI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssHII
BstXI BstYI BtgI BtgZI BtsI DraI EaeI EarI
EcoICRI Eco57MI EcoRI EcoRV FalI HaeII Hin4I HincII
MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI
PciI PfoI PmlI PsiI PstI PvuII SacI SapI
SbfI SfcI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AloI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BciVI BclI BfrBI BglI BmgBI BmtI Bpu10I BsaBI
BsiEI BsiWI BspHI BsrFI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoO109I FseI FspI FspAI HindIII HpaI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI XbaI XcmI XhoI XmaI