Restriction map for
pF1KB4948
(Linear) (Six-base) MAPSORT of: KIBB4948.seq Check: 1683 from: 1 to: 1740
KIBB4948.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 111 963 1740
Size: 111 852 777
Fragments arranged by size:
852 777 111
AatII G_ACGT'C
Cuts at: 0 21 1740
Size: 21 1719
AccI GT'mk_AC
Cuts at: 0 593 1740
Size: 593 1147
AleI CACnn'nnGTG
Cuts at: 0 814 1740
Size: 814 926
AlwNI CAG_nnn'CTG
Cuts at: 0 141 958 1404 1623 1740
Size: 141 817 446 219 117
Fragments arranged by size:
817 446 219 141 117
ApoI r'AATT_y
Cuts at: 0 3 373 1740
Size: 3 370 1367
Fragments arranged by size:
1367 370 3
AvaI C'yCGr_G
Cuts at: 0 1463 1740
Size: 1463 277
BamHI G'GATC_C
Cuts at: 0 166 275 895 1740
Size: 166 109 620 845
Fragments arranged by size:
845 620 166 109
BanI G'GyrC_C
Cuts at: 0 128 1507 1740
Size: 128 1379 233
Fragments arranged by size:
1379 233 128
BanII G_rGCy'C
Cuts at: 0 1233 1411 1740
Size: 1233 178 329
Fragments arranged by size:
1233 329 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 1682 1740
Size: 530 1152 58
Fragments arranged by size:
1152 530 58
BbvCI CC'TCA_GC
Cuts at: 0 1280 1343 1636 1740
Size: 1280 63 293 104
Fragments arranged by size:
1280 293 104 63
BglI GCCn_nnn'nGGC
Cuts at: 0 214 1054 1740
Size: 214 840 686
Fragments arranged by size:
840 686 214
BglII A'GATC_T
Cuts at: 0 638 1354 1740
Size: 638 716 386
Fragments arranged by size:
716 638 386
BmrI ACTGGGnnnn_n'
Cuts at: 0 1407 1740
Size: 1407 333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 174 224 988 1740
Size: 146 28 50 764 752
Fragments arranged by size:
764 752 146 50 28
Bpu10I CC'TnA_GC
Cuts at: 0 1280 1343 1405 1636 1740
Size: 1280 63 62 231 104
Fragments arranged by size:
1280 231 104 63 62
BsaBI GATnn'nnATC
Cuts at: 0 352 1740
Size: 352 1388
BsaHI Gr'CG_yC
Cuts at: 0 18 1740
Size: 18 1722
BsaWI w'CCGG_w
Cuts at: 0 1417 1740
Size: 1417 323
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1115 1145 1480 1510 1740
Size: 1115 30 335 30 230
Fragments arranged by size:
1115 335 230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 147 1331 1740
Size: 138 9 1184 409
Fragments arranged by size:
1184 409 138 9
BseYI C'CCAG_C
Cuts at: 0 134 744 1268 1272 1295 1559 1655 1740
Size: 134 610 524 4 23 264 96 85
Fragments arranged by size:
610 524 264 134 96 85 23 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 1740
Size: 1561 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 1740
Size: 25 1715
Bsp1286I G_dGCh'C
Cuts at: 0 1233 1411 1740
Size: 1233 178 329
Fragments arranged by size:
1233 329 178
BspEI T'CCGG_A
Cuts at: 0 1417 1740
Size: 1417 323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 80 111 420 963 1740
Size: 56 24 31 309 543 777
Fragments arranged by size:
777 543 309 56 31 24
BstEII G'GTnAC_C
Cuts at: 0 1439 1740
Size: 1439 301
BstXI CCAn_nnnn'nTGG
Cuts at: 0 892 1357 1740
Size: 892 465 383
Fragments arranged by size:
892 465 383
BstYI r'GATC_y
Cuts at: 0 166 275 353 463 638 895 1354 1420
Size: 166 109 78 110 175 257 459 66
Cuts at: 1420 1740
Size: 320
Fragments arranged by size:
459 320 257 175 166 110 109 78
66
Bsu36I CC'TnA_GG
Cuts at: 0 212 222 1708 1740
Size: 212 10 1486 32
Fragments arranged by size:
1486 212 32 10
BtgI C'CryG_G
Cuts at: 0 116 324 824 1740
Size: 116 208 500 916
Fragments arranged by size:
916 500 208 116
ClaI AT'CG_AT
Cuts at: 0 290 1740
Size: 290 1450
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 240 275 1740
Size: 240 35 1465
Fragments arranged by size:
1465 240 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 430 1165 1740
Size: 430 735 575
Fragments arranged by size:
735 575 430
EaeI y'GGCC_r
Cuts at: 0 41 827 977 1154 1740
Size: 41 786 150 177 586
Fragments arranged by size:
786 586 177 150 41
EarI CTCTTCn'nnn_
Cuts at: 0 1130 1565 1740
Size: 1130 435 175
Fragments arranged by size:
1130 435 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 481 1740
Size: 481 1259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 174 224 988 1740
Size: 146 28 50 764 752
Fragments arranged by size:
764 752 146 50 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1283 1313 1740
Size: 1283 30 427
Fragments arranged by size:
1283 427 30
EcoO109I rG'GnC_Cy
Cuts at: 0 207 244 655 1276 1321 1664 1740
Size: 207 37 411 621 45 343 76
Fragments arranged by size:
621 411 343 207 76 45 37
EcoRI G'AATT_C
Cuts at: 0 373 1740
Size: 373 1367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 1740
Size: 495 1245
HaeII r_GCGC'y
Cuts at: 0 1210 1740
Size: 1210 530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 260 292 1000 1032 1070 1102
Size: 222 32 6 32 708 32 38 32
Cuts at: 1102 1740
Size: 638
Fragments arranged by size:
708 638 222 38 32 32 32 32
6
HincII GTy'rAC
Cuts at: 0 576 1740
Size: 576 1164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1670 1740
Size: 923 747 70
Fragments arranged by size:
923 747 70
MscI TGG'CCA
Cuts at: 0 43 829 979 1740
Size: 43 786 150 761
Fragments arranged by size:
786 761 150 43
MslI CAynn'nnrTG
Cuts at: 0 814 1740
Size: 814 926
MspA1I CmG'CkG
Cuts at: 0 1013 1116 1149 1272 1740
Size: 1013 103 33 123 468
Fragments arranged by size:
1013 468 123 103 33
NcoI C'CATG_G
Cuts at: 0 116 324 824 1740
Size: 116 208 500 916
Fragments arranged by size:
916 500 208 116
NspI r_CATG'y
Cuts at: 0 429 502 754 1132 1740
Size: 429 73 252 378 608
Fragments arranged by size:
608 429 378 252 73
PasI CC'CwG_GG
Cuts at: 0 516 614 651 1258 1740
Size: 516 98 37 607 482
Fragments arranged by size:
607 516 482 98 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 651 1740
Size: 141 510 1089
Fragments arranged by size:
1089 510 141
PfoI T'CCnGG_A
Cuts at: 0 162 800 1740
Size: 162 638 940
Fragments arranged by size:
940 638 162
PpuMI rG'GwC_Cy
Cuts at: 0 244 655 1664 1740
Size: 244 411 1009 76
Fragments arranged by size:
1009 411 244 76
PstI C_TGCA'G
Cuts at: 0 554 607 1740
Size: 554 53 1133
Fragments arranged by size:
1133 554 53
PvuII CAG'CTG
Cuts at: 0 1013 1149 1272 1740
Size: 1013 136 123 468
Fragments arranged by size:
1013 468 136 123
SanDI GG'GwC_CC
Cuts at: 0 1664 1740
Size: 1664 76
SfcI C'TryA_G
Cuts at: 0 504 550 603 1740
Size: 504 46 53 1137
Fragments arranged by size:
1137 504 53 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 214 1740
Size: 214 1526
SmaI CCC'GGG
Cuts at: 0 1465 1740
Size: 1465 275
SphI G_CATG'C
Cuts at: 0 502 1740
Size: 502 1238
StuI AGG'CCT
Cuts at: 0 853 1495 1740
Size: 853 642 245
Fragments arranged by size:
853 642 245
StyI C'CwwG_G
Cuts at: 0 116 324 824 1225 1685 1740
Size: 116 208 500 401 460 55
Fragments arranged by size:
500 460 401 208 116 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 606 993 1740
Size: 606 387 747
Fragments arranged by size:
747 606 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1115 1147 1656 1688 1740
Size: 1115 32 509 32 52
Fragments arranged by size:
1115 509 52 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 1069 1740
Size: 279 790 671
Fragments arranged by size:
790 671 279
XmaI C'CCGG_G
Cuts at: 0 1463 1740
Size: 1463 277
XmnI GAAnn'nnTTC
Cuts at: 0 319 1740
Size: 319 1421
ZraI GAC'GTC
Cuts at: 0 19 1740
Size: 19 1721
Enzymes that do cut:
AarI AatII AccI AleI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BglI BglII BmrI BpmI
Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmBI Bsp1286I BspEI BspMI BstEII BstXI BstYI Bsu36I
BtgI ClaI CspCI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpuMI PstI PvuII SanDI SfcI SfiI SmaI SphI
StuI StyI TaqII TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BlpI Bme1580I
BmgBI BmtI BplI BpuEI BsaI BsaAI BsiEI BsiHKAI
BsiWI BsmI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I BtgZI BtsI DraI DraIII
EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI
TatI Tth111I XbaI XhoI