Restriction map for
pF1KB4917
(Linear) (Six-base) MAPSORT of: KIBB4917.seq Check: 9547 from: 1 to: 969
KIBB4917.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 79 352 969
Size: 79 273 617
Fragments arranged by size:
617 273 79
AccI GT'mk_AC
Cuts at: 0 914 969
Size: 914 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 751 874 969
Size: 400 351 123 95
Fragments arranged by size:
400 351 123 95
AflIII A'CryG_T
Cuts at: 0 677 969
Size: 677 292
AleI CACnn'nnGTG
Cuts at: 0 73 969
Size: 73 896
AlwNI CAG_nnn'CTG
Cuts at: 0 300 969
Size: 300 669
ApaI G_GGCC'C
Cuts at: 0 841 969
Size: 841 128
AvaI C'yCGr_G
Cuts at: 0 232 568 969
Size: 232 336 401
Fragments arranged by size:
401 336 232
BamHI G'GATC_C
Cuts at: 0 800 969
Size: 800 169
BanI G'GyrC_C
Cuts at: 0 340 463 859 969
Size: 340 123 396 110
Fragments arranged by size:
396 340 123 110
BanII G_rGCy'C
Cuts at: 0 841 969
Size: 841 128
BbeI G_GCGC'C
Cuts at: 0 863 969
Size: 863 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 754 969
Size: 754 215
BglI GCCn_nnn'nGGC
Cuts at: 0 721 969
Size: 721 248
Bme1580I G_kGCm'C
Cuts at: 0 468 841 969
Size: 468 373 128
Fragments arranged by size:
468 373 128
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 858 969
Size: 858 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 742 969
Size: 470 272 227
Fragments arranged by size:
470 272 227
Bpu10I CC'TnA_GC
Cuts at: 0 583 969
Size: 583 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 969
Size: 559 410
BsaAI yAC'GTr
Cuts at: 0 678 969
Size: 678 291
BsaHI Gr'CG_yC
Cuts at: 0 652 776 860 969
Size: 652 124 84 109
Fragments arranged by size:
652 124 109 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 194 224 969
Size: 194 30 745
Fragments arranged by size:
745 194 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 969
Size: 673 296
BseYI C'CCAG_C
Cuts at: 0 467 512 639 703 942 969
Size: 467 45 127 64 239 27
Fragments arranged by size:
467 239 127 64 45 27
Bsp1286I G_dGCh'C
Cuts at: 0 468 841 969
Size: 468 373 128
Fragments arranged by size:
468 373 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 79 352 536 969
Size: 79 273 184 433
Fragments arranged by size:
433 273 184 79
BsrBI CCG'CTC
Cuts at: 0 173 618 969
Size: 173 445 351
Fragments arranged by size:
445 351 173
BsrDI GCAATG_nn'
Cuts at: 0 407 545 969
Size: 407 138 424
Fragments arranged by size:
424 407 138
BsrFI r'CCGG_y
Cuts at: 0 550 691 969
Size: 550 141 278
Fragments arranged by size:
550 278 141
BsrGI T'GTAC_A
Cuts at: 0 134 969
Size: 134 835
BssHII G'CGCG_C
Cuts at: 0 872 969
Size: 872 97
BssSI C'ACGA_G
Cuts at: 0 138 832 969
Size: 138 694 137
Fragments arranged by size:
694 138 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 249 369 969
Size: 90 159 120 600
Fragments arranged by size:
600 159 120 90
BstYI r'GATC_y
Cuts at: 0 383 425 800 821 969
Size: 383 42 375 21 148
Fragments arranged by size:
383 375 148 42 21
BtgI C'CryG_G
Cuts at: 0 368 503 540 969
Size: 368 135 37 429
Fragments arranged by size:
429 368 135 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 33 867 885 912 969
Size: 33 834 18 27 57
Fragments arranged by size:
834 57 33 27 18
EaeI y'GGCC_r
Cuts at: 0 92 123 506 689 722 969
Size: 92 31 383 183 33 247
Fragments arranged by size:
383 247 183 92 33 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 470 742 751 874 969
Size: 400 70 272 9 123 95
Fragments arranged by size:
400 272 123 95 70 9
EcoO109I rG'GnC_Cy
Cuts at: 0 837 838 957 969
Size: 837 1 119 12
Fragments arranged by size:
837 119 12 1
HaeII r_GCGC'y
Cuts at: 0 863 969
Size: 863 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 649 681 778 810 969
Size: 263 32 354 32 97 32 159
Fragments arranged by size:
354 263 159 97 32 32 32
HincII GTy'rAC
Cuts at: 0 729 780 969
Size: 729 51 189
Fragments arranged by size:
729 189 51
KasI G'GCGC_C
Cuts at: 0 859 969
Size: 859 110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 294 969
Size: 173 121 675
Fragments arranged by size:
675 173 121
MscI TGG'CCA
Cuts at: 0 94 969
Size: 94 875
MslI CAynn'nnrTG
Cuts at: 0 73 142 367 373 969
Size: 73 69 225 6 596
Fragments arranged by size:
596 225 73 69 6
MspA1I CmG'CkG
Cuts at: 0 390 490 516 639 718 969
Size: 390 100 26 123 79 251
Fragments arranged by size:
390 251 123 100 79 26
NaeI GCC'GGC
Cuts at: 0 693 969
Size: 693 276
NarI GG'CG_CC
Cuts at: 0 860 969
Size: 860 109
NcoI C'CATG_G
Cuts at: 0 368 969
Size: 368 601
NgoMIV G'CCGG_C
Cuts at: 0 691 969
Size: 691 278
NspI r_CATG'y
Cuts at: 0 147 195 969
Size: 147 48 774
Fragments arranged by size:
774 147 48
PasI CC'CwG_GG
Cuts at: 0 438 456 969
Size: 438 18 513
Fragments arranged by size:
513 438 18
PfoI T'CCnGG_A
Cuts at: 0 803 969
Size: 803 166
PpuMI rG'GwC_Cy
Cuts at: 0 957 969
Size: 957 12
PshAI GACnn'nnGTC
Cuts at: 0 223 969
Size: 223 746
PspOMI G'GGCC_C
Cuts at: 0 837 969
Size: 837 132
PstI C_TGCA'G
Cuts at: 0 110 395 956 969
Size: 110 285 561 13
Fragments arranged by size:
561 285 110 13
PvuII CAG'CTG
Cuts at: 0 390 490 516 639 969
Size: 390 100 26 123 330
Fragments arranged by size:
390 330 123 100 26
SfcI C'TryA_G
Cuts at: 0 106 391 952 969
Size: 106 285 561 17
Fragments arranged by size:
561 285 106 17
SfoI GGC'GCC
Cuts at: 0 861 969
Size: 861 108
StyI C'CwwG_G
Cuts at: 0 368 969
Size: 368 601
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 526 969
Size: 526 443
TatI w'GTAC_w
Cuts at: 0 134 608 969
Size: 134 474 361
Fragments arranged by size:
474 361 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 194 226 969
Size: 194 32 743
Fragments arranged by size:
743 194 32
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BglI Bme1580I BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstXI BstYI BtgI BtgZI EaeI Eco57MI EcoO109I HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PfoI PpuMI PshAI
PspOMI PstI PvuII SfcI SfoI StyI TaqII TatI
TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BpuEI BsaBI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BstAPI BstBI BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI