(Linear) (Six-base) MAPSORT of: KIBB4911.seq  Check: 9727  from: 1  to: 582



KIBB4911.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     582 
   Size:        542      40
 
ApoI r'AATT_y

Cuts at:      0     163     494     582 
   Size:        163     331      88

  Fragments arranged by size: 

                 331     163      88
 
AvaI C'yCGr_G

Cuts at:      0      31     582 
   Size:         31     551
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      29      62     582 
   Size:         29      33     520

  Fragments arranged by size: 

                 520      33      29
 
BanII G_rGCy'C

Cuts at:      0      17     582 
   Size:         17     565
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     555     582 
   Size:         86     469      27

  Fragments arranged by size: 

                 469      86      27
 
BbvCI CC'TCA_GC

Cuts at:      0       7     582 
   Size:          7     575
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      30     171     582 
   Size:         30     141     411

  Fragments arranged by size: 

                 411     141      30
 
Bpu10I CC'TnA_GC

Cuts at:      0       7     207     582 
   Size:          7     200     375

  Fragments arranged by size: 

                 375     200       7
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     430     582 
   Size:        430     152
 
BsaHI Gr'CG_yC

Cuts at:      0     509     582 
   Size:        509      73
 
BsiHKAI G_wGCw'C

Cuts at:      0      17     582 
   Size:         17     565
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     582 
   Size:         17     565
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     524     582 
   Size:        524      58
 
BsrDI GCAATG_nn'

Cuts at:      0     341     582 
   Size:        341     241
 
ClaI AT'CG_AT

Cuts at:      0     368     582 
   Size:        368     214
 
EarI CTCTTCn'nnn_

Cuts at:      0     263     582 
   Size:        263     319
 
EcoICRI GAG'CTC

Cuts at:      0      15     582 
   Size:         15     567
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     582 
   Size:        542      40
 
EcoRI G'AATT_C

Cuts at:      0     494     582 
   Size:        494      88
 
EcoRV GAT'ATC

Cuts at:      0     258     582 
   Size:        258     324
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     572     582 
   Size:        572      10
 
HaeII r_GCGC'y

Cuts at:      0       7     582 
   Size:          7     575
 
MspA1I CmG'CkG

Cuts at:      0     184     582 
   Size:        184     398
 
PstI C_TGCA'G

Cuts at:      0     240     582 
   Size:        240     342
 
PvuII CAG'CTG

Cuts at:      0     184     582 
   Size:        184     398
 
SacI G_AGCT'C

Cuts at:      0      17     582 
   Size:         17     565
 
SfcI C'TryA_G

Cuts at:      0     236     582 
   Size:        236     346
 
SspI AAT'ATT

Cuts at:      0     330     582 
   Size:        330     252
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     285     582 
   Size:        285     297
 
TatI w'GTAC_w

Cuts at:      0     218     582 
   Size:        218     364
 
XmnI GAAnn'nnTTC

Cuts at:      0     197     582 
   Size:        197     385
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BaeI    BanII     BbsI    BbvCI    BciVI
   Bpu10I     BsaI    BsaHI  BsiHKAI Bsp1286I    BspMI    BsrDI     ClaI
     EarI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI    HaeII   MspA1I
     PstI    PvuII     SacI     SfcI     SspI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII    BamHI     BanI     BbeI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI