Restriction map for
pF1KB4908
(Linear) (Six-base) MAPSORT of: KIBB4908.seq Check: 6607 from: 1 to: 1932
KIBB4908.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 448 510 1058 1201 1781 1932
Size: 448 62 548 143 580 151
Fragments arranged by size:
580 548 448 151 143 62
AatII G_ACGT'C
Cuts at: 0 521 1932
Size: 521 1411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 921 1005 1341 1425 1509 1593 1932
Size: 373 548 84 336 84 84 84 339
Fragments arranged by size:
548 373 339 336 84 84 84 84
AflIII A'CryG_T
Cuts at: 0 567 1367 1932
Size: 567 800 565
Fragments arranged by size:
800 567 565
AgeI A'CCGG_T
Cuts at: 0 1079 1567 1932
Size: 1079 488 365
Fragments arranged by size:
1079 488 365
AleI CACnn'nnGTG
Cuts at: 0 1568 1932
Size: 1568 364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1051 1066 1932
Size: 1051 15 866
Fragments arranged by size:
1051 866 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 724 756 1228 1260 1932
Size: 724 32 472 32 672
Fragments arranged by size:
724 672 472 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1007 1259 1728 1932
Size: 1007 252 469 204
Fragments arranged by size:
1007 469 252 204
ApaI G_GGCC'C
Cuts at: 0 335 1932
Size: 335 1597
ApaLI G'TGCA_C
Cuts at: 0 961 1057 1381 1465 1549 1932
Size: 961 96 324 84 84 383
Fragments arranged by size:
961 383 324 96 84 84
ApoI r'AATT_y
Cuts at: 0 532 1902 1932
Size: 532 1370 30
Fragments arranged by size:
1370 532 30
AvaI C'yCGr_G
Cuts at: 0 22 76 574 1932
Size: 22 54 498 1358
Fragments arranged by size:
1358 498 54 22
AvrII C'CTAG_G
Cuts at: 0 243 1932
Size: 243 1689
BamHI G'GATC_C
Cuts at: 0 48 1839 1932
Size: 48 1791 93
Fragments arranged by size:
1791 93 48
BanI G'GyrC_C
Cuts at: 0 360 449 492 724 1932
Size: 360 89 43 232 1208
Fragments arranged by size:
1208 360 232 89 43
BanII G_rGCy'C
Cuts at: 0 23 335 410 781 1705 1932
Size: 23 312 75 371 924 227
Fragments arranged by size:
924 371 312 227 75 23
BbeI G_GCGC'C
Cuts at: 0 453 496 728 1932
Size: 453 43 232 1204
Fragments arranged by size:
1204 453 232 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 91 1932
Size: 34 57 1841
Fragments arranged by size:
1841 57 34
BlpI GC'TnA_GC
Cuts at: 0 1039 1777 1932
Size: 1039 738 155
Fragments arranged by size:
1039 738 155
Bme1580I G_kGCm'C
Cuts at: 0 335 365 965 1061 1385 1469 1553 1932
Size: 335 30 600 96 324 84 84 379
Fragments arranged by size:
600 379 335 324 96 84 84 30
BmgBI CAC'GTC
Cuts at: 0 1836 1932
Size: 1836 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 895 1719 1932
Size: 879 16 824 213
Fragments arranged by size:
879 824 213 16
BsaAI yAC'GTr
Cuts at: 0 1791 1932
Size: 1791 141
BsaHI Gr'CG_yC
Cuts at: 0 450 493 518 725 1932
Size: 450 43 25 207 1207
Fragments arranged by size:
1207 450 207 43 25
BsaWI w'CCGG_w
Cuts at: 0 1079 1567 1932
Size: 1079 488 365
Fragments arranged by size:
1079 488 365
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 724 754 1648 1678 1932
Size: 724 30 894 30 254
Fragments arranged by size:
894 724 254 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 310 486 771 1932
Size: 178 132 176 285 1161
Fragments arranged by size:
1161 285 178 176 132
BseYI C'CCAG_C
Cuts at: 0 560 1806 1932
Size: 560 1246 126
Fragments arranged by size:
1246 560 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 674 778 1020 1272 1608 1714 1832
Size: 241 433 104 242 252 336 106 118
Cuts at: 1832 1932
Size: 100
Fragments arranged by size:
433 336 252 242 241 118 106 104
100
BsiHKAI G_wGCw'C
Cuts at: 0 465 781 865 881 965 1061 1385 1469
Size: 465 316 84 16 84 96 324 84
Cuts at: 1469 1553 1705 1721 1932
Size: 84 152 16 211
Fragments arranged by size:
465 324 316 211 152 96 84 84
84 84 16 16
BsmI GAATG_Cn'
Cuts at: 0 844 1932
Size: 844 1088
Bsp1286I G_dGCh'C
Cuts at: 0 23 335 365 410 465 781 865 881
Size: 23 312 30 45 55 316 84 16
Cuts at: 881 965 1061 1385 1469 1553 1705 1721 1932
Size: 84 96 324 84 84 152 16 211
Fragments arranged by size:
324 316 312 211 152 96 84 84
84 84 55 45 30 23 16 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 448 510 1058 1201 1382 1781 1932
Size: 448 62 548 143 181 399 151
Fragments arranged by size:
548 448 399 181 151 143 62
BsrBI CCG'CTC
Cuts at: 0 420 987 1283 1932
Size: 420 567 296 649
Fragments arranged by size:
649 567 420 296
BsrFI r'CCGG_y
Cuts at: 0 396 707 797 881 895 1063 1079 1385
Size: 396 311 90 84 14 168 16 306
Cuts at: 1385 1469 1567 1932
Size: 84 98 365
Fragments arranged by size:
396 365 311 306 168 98 90 84
84 16 14
BssSI C'ACGA_G
Cuts at: 0 1378 1932
Size: 1378 554
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1007 1259 1427 1595 1819 1932
Size: 1007 252 168 168 224 113
Fragments arranged by size:
1007 252 224 168 168 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 1932
Size: 873 1059
BstYI r'GATC_y
Cuts at: 0 5 48 1223 1839 1932
Size: 5 43 1175 616 93
Fragments arranged by size:
1175 616 93 43 5
Bsu36I CC'TnA_GG
Cuts at: 0 541 1932
Size: 541 1391
BtgI C'CryG_G
Cuts at: 0 301 506 1932
Size: 301 205 1426
Fragments arranged by size:
1426 301 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 848 1932
Size: 848 1084
BtsI GCAGTG_nn'
Cuts at: 0 253 1932
Size: 253 1679
EaeI y'GGCC_r
Cuts at: 0 1751 1932
Size: 1751 181
EarI CTCTTCn'nnn_
Cuts at: 0 559 1932
Size: 559 1373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 760 1932
Size: 760 1172
EcoICRI GAG'CTC
Cuts at: 0 779 1703 1932
Size: 779 924 229
Fragments arranged by size:
924 779 229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 879 895 921 1005 1341 1425 1509
Size: 373 506 16 26 84 336 84 84
Cuts at: 1509 1593 1719 1932
Size: 84 126 213
Fragments arranged by size:
506 373 336 213 126 84 84 84
84 26 16
EcoO109I rG'GnC_Cy
Cuts at: 0 194 247 331 332 583 636 1932
Size: 194 53 84 1 251 53 1296
Fragments arranged by size:
1296 251 194 84 53 53 1
EcoRI G'AATT_C
Cuts at: 0 532 1932
Size: 532 1400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 927 1932
Size: 927 1005
HaeII r_GCGC'y
Cuts at: 0 453 496 728 804 1007 1932
Size: 453 43 232 76 203 925
Fragments arranged by size:
925 453 232 203 76 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 259 264 291 296 1932
Size: 70 32 157 5 27 5 1636
Fragments arranged by size:
1636 157 70 32 27 5 5
HincII GTy'rAC
Cuts at: 0 175 1932
Size: 175 1757
KasI G'GCGC_C
Cuts at: 0 449 492 724 1932
Size: 449 43 232 1208
Fragments arranged by size:
1208 449 232 43
MluI A'CGCG_T
Cuts at: 0 567 1367 1932
Size: 567 800 565
Fragments arranged by size:
800 567 565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1495 1932
Size: 197 1298 437
Fragments arranged by size:
1298 437 197
MscI TGG'CCA
Cuts at: 0 1753 1932
Size: 1753 179
MslI CAynn'nnrTG
Cuts at: 0 1568 1932
Size: 1568 364
MspA1I CmG'CkG
Cuts at: 0 941 1025 1232 1361 1400 1529 1932
Size: 941 84 207 129 39 129 403
Fragments arranged by size:
941 403 207 129 129 84 39
NaeI GCC'GGC
Cuts at: 0 709 1932
Size: 709 1223
NarI GG'CG_CC
Cuts at: 0 450 493 725 1932
Size: 450 43 232 1207
Fragments arranged by size:
1207 450 232 43
NcoI C'CATG_G
Cuts at: 0 301 506 1932
Size: 301 205 1426
Fragments arranged by size:
1426 301 205
NgoMIV G'CCGG_C
Cuts at: 0 707 1932
Size: 707 1225
NspI r_CATG'y
Cuts at: 0 312 1047 1932
Size: 312 735 885
Fragments arranged by size:
885 735 312
PasI CC'CwG_GG
Cuts at: 0 488 1911 1932
Size: 488 1423 21
Fragments arranged by size:
1423 488 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 512 1932
Size: 512 1420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 724 756 1932
Size: 724 32 1176
Fragments arranged by size:
1176 724 32
PpuMI rG'GwC_Cy
Cuts at: 0 247 583 1932
Size: 247 336 1349
Fragments arranged by size:
1349 336 247
PspOMI G'GGCC_C
Cuts at: 0 331 1932
Size: 331 1601
PstI C_TGCA'G
Cuts at: 0 470 1030 1507 1932
Size: 470 560 477 425
Fragments arranged by size:
560 477 470 425
PvuII CAG'CTG
Cuts at: 0 941 1025 1361 1529 1932
Size: 941 84 336 168 403
Fragments arranged by size:
941 403 336 168 84
SacI G_AGCT'C
Cuts at: 0 781 1705 1932
Size: 781 924 227
Fragments arranged by size:
924 781 227
SanDI GG'GwC_CC
Cuts at: 0 583 1932
Size: 583 1349
SfcI C'TryA_G
Cuts at: 0 466 524 1026 1503 1932
Size: 466 58 502 477 429
Fragments arranged by size:
502 477 466 429 58
SfoI GGC'GCC
Cuts at: 0 451 494 726 1932
Size: 451 43 232 1206
Fragments arranged by size:
1206 451 232 43
SgrAI Cr'CCGG_yG
Cuts at: 0 797 881 895 1063 1385 1469 1567 1932
Size: 797 84 14 168 322 84 98 365
Fragments arranged by size:
797 365 322 168 98 84 84 14
SmlI C'TyrA_G
Cuts at: 0 76 1932
Size: 76 1856
SphI G_CATG'C
Cuts at: 0 312 1047 1932
Size: 312 735 885
Fragments arranged by size:
885 735 312
StuI AGG'CCT
Cuts at: 0 850 934 1018 1102 1186 1270 1522 1932
Size: 850 84 84 84 84 84 252 410
Fragments arranged by size:
850 410 252 84 84 84 84 84
StyI C'CwwG_G
Cuts at: 0 116 243 301 506 632 1748 1932
Size: 116 127 58 205 126 1116 184
Fragments arranged by size:
1116 205 184 127 126 116 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 1798 1932
Size: 172 1626 134
Fragments arranged by size:
1626 172 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1851 1932
Size: 1851 81
XhoI C'TCGA_G
Cuts at: 0 76 1932
Size: 76 1856
XmnI GAAnn'nnTTC
Cuts at: 0 766 907 934 1018 1270 1327 1354 1411
Size: 766 141 27 84 252 57 27 57
Cuts at: 1411 1495 1522 1932
Size: 84 27 410
Fragments arranged by size:
766 410 252 141 84 84 57 57
27 27 27
ZraI GAC'GTC
Cuts at: 0 519 1932
Size: 519 1413
Enzymes that do cut:
AarI AatII AcuI AflIII AgeI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BlpI Bme1580I BmgBI BpmI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PpiI PpuMI PspOMI PstI
PvuII SacI SanDI SfcI SfoI SgrAI SmlI SphI
StuI StyI TaqII XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AhdI AscI AseI
AsiSI BaeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI
TatI TstI Tth111I XbaI XmaI