Restriction map for
pF1KB4902
(Linear) (Six-base) MAPSORT of: KIBB4902.seq Check: 2922 from: 1 to: 2232
KIBB4902.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 1271 2232
Size: 1271 961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 499 947 1939 2232
Size: 166 333 448 992 293
Fragments arranged by size:
992 448 333 293 166
AflII C'TTAA_G
Cuts at: 0 2196 2232
Size: 2196 36
AflIII A'CryG_T
Cuts at: 0 753 2232
Size: 753 1479
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 2232
Size: 1062 1170
ApoI r'AATT_y
Cuts at: 0 358 567 676 1302 2182 2232
Size: 358 209 109 626 880 50
Fragments arranged by size:
880 626 358 209 109 50
AvaI C'yCGr_G
Cuts at: 0 710 1544 2232
Size: 710 834 688
Fragments arranged by size:
834 710 688
BamHI G'GATC_C
Cuts at: 0 465 2160 2232
Size: 465 1695 72
Fragments arranged by size:
1695 465 72
BanI G'GyrC_C
Cuts at: 0 1896 2232
Size: 1896 336
BanII G_rGCy'C
Cuts at: 0 845 2232
Size: 845 1387
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 1167 1651 2232
Size: 154 1013 484 581
Fragments arranged by size:
1013 581 484 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 551 585 2232
Size: 551 34 1647
Fragments arranged by size:
1647 551 34
BglII A'GATC_T
Cuts at: 0 1128 1697 1760 2232
Size: 1128 569 63 472
Fragments arranged by size:
1128 569 472 63
Bme1580I G_kGCm'C
Cuts at: 0 2003 2232
Size: 2003 229
BmrI ACTGGGnnnn_n'
Cuts at: 0 1338 1531 2232
Size: 1338 193 701
Fragments arranged by size:
1338 701 193
BmtI G_CTAG'C
Cuts at: 0 2216 2232
Size: 2216 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 134 2232
Size: 134 2098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 2232
Size: 707 1525
BsaI GGTCTCn'nnnn_
Cuts at: 0 1288 2232
Size: 1288 944
BsaAI yAC'GTr
Cuts at: 0 1998 2232
Size: 1998 234
BsaBI GATnn'nnATC
Cuts at: 0 2116 2232
Size: 2116 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 933 963 2232
Size: 933 30 1269
Fragments arranged by size:
1269 933 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 946 1445 1622 1892 2232
Size: 946 499 177 270 340
Fragments arranged by size:
946 499 340 270 177
BseYI C'CCAG_C
Cuts at: 0 208 1784 2232
Size: 208 1576 448
Fragments arranged by size:
1576 448 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1594 2232
Size: 83 1511 638
Fragments arranged by size:
1511 638 83
BsmI GAATG_Cn'
Cuts at: 0 181 837 2232
Size: 181 656 1395
Fragments arranged by size:
1395 656 181
Bsp1286I G_dGCh'C
Cuts at: 0 845 2003 2232
Size: 845 1158 229
Fragments arranged by size:
1158 845 229
BspHI T'CATG_A
Cuts at: 0 1038 2232
Size: 1038 1194
BsrDI GCAATG_nn'
Cuts at: 0 176 2006 2232
Size: 176 1830 226
Fragments arranged by size:
1830 226 176
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1666 2232
Size: 1666 566
BstEII G'GTnAC_C
Cuts at: 0 1531 2232
Size: 1531 701
BstYI r'GATC_y
Cuts at: 0 465 1128 1697 1760 2160 2232
Size: 465 663 569 63 400 72
Fragments arranged by size:
663 569 465 400 72 63
Bsu36I CC'TnA_GG
Cuts at: 0 1904 2232
Size: 1904 328
BtgI C'CryG_G
Cuts at: 0 461 1289 2232
Size: 461 828 943
Fragments arranged by size:
943 828 461
BtsI GCAGTG_nn'
Cuts at: 0 1349 2232
Size: 1349 883
DrdI GACnn_nn'nnGTC
Cuts at: 0 1048 2232
Size: 1048 1184
EaeI y'GGCC_r
Cuts at: 0 93 1286 2232
Size: 93 1193 946
Fragments arranged by size:
1193 946 93
EarI CTCTTCn'nnn_
Cuts at: 0 923 1151 1880 2232
Size: 923 228 729 352
Fragments arranged by size:
923 729 352 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 499 707 947 1939 2232
Size: 166 333 208 240 992 293
Fragments arranged by size:
992 333 293 240 208 166
EcoNI CCTnn'n_nnAGG
Cuts at: 0 494 2232
Size: 494 1738
EcoO109I rG'GnC_Cy
Cuts at: 0 113 1627 2232
Size: 113 1514 605
Fragments arranged by size:
1514 605 113
EcoRV GAT'ATC
Cuts at: 0 1461 2232
Size: 1461 771
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 819 1779 1969 2232
Size: 819 960 190 263
Fragments arranged by size:
960 819 263 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 73 105 1233 1265 1689 1721
Size: 31 32 10 32 1128 32 424 32
Cuts at: 1721 2232
Size: 511
Fragments arranged by size:
1128 511 424 32 32 32 32 31
10
HincII GTy'rAC
Cuts at: 0 1053 2232
Size: 1053 1179
HindIII A'AGCT_T
Cuts at: 0 427 1915 2033 2232
Size: 427 1488 118 199
Fragments arranged by size:
1488 427 199 118
MfeI C'AATT_G
Cuts at: 0 57 2078 2232
Size: 57 2021 154
Fragments arranged by size:
2021 154 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 513 717 1703 2009 2232
Size: 350 163 204 986 306 223
Fragments arranged by size:
986 350 306 223 204 163
MscI TGG'CCA
Cuts at: 0 95 2232
Size: 95 2137
MslI CAynn'nnrTG
Cuts at: 0 1111 1354 1996 2232
Size: 1111 243 642 236
Fragments arranged by size:
1111 642 243 236
MspA1I CmG'CkG
Cuts at: 0 2025 2232
Size: 2025 207
NcoI C'CATG_G
Cuts at: 0 461 2232
Size: 461 1771
NheI G'CTAG_C
Cuts at: 0 2212 2232
Size: 2212 20
NspI r_CATG'y
Cuts at: 0 18 103 757 2232
Size: 18 85 654 1475
Fragments arranged by size:
1475 654 85 18
PciI A'CATG_T
Cuts at: 0 753 2232
Size: 753 1479
PflMI CCAn_nnn'nTGG
Cuts at: 0 790 1474 2232
Size: 790 684 758
Fragments arranged by size:
790 758 684
PmlI CAC'GTG
Cuts at: 0 1998 2232
Size: 1998 234
PpuMI rG'GwC_Cy
Cuts at: 0 113 2232
Size: 113 2119
PspXI vC'TCGA_Gb
Cuts at: 0 1544 2232
Size: 1544 688
PstI C_TGCA'G
Cuts at: 0 308 2232
Size: 308 1924
PvuII CAG'CTG
Cuts at: 0 2025 2232
Size: 2025 207
SapI GCTCTTCn'nnn_
Cuts at: 0 1151 1880 2232
Size: 1151 729 352
Fragments arranged by size:
1151 729 352
ScaI AGT'ACT
Cuts at: 0 1380 2232
Size: 1380 852
SfcI C'TryA_G
Cuts at: 0 35 304 1210 2152 2232
Size: 35 269 906 942 80
Fragments arranged by size:
942 906 269 80 35
SmlI C'TyrA_G
Cuts at: 0 1544 2196 2232
Size: 1544 652 36
Fragments arranged by size:
1544 652 36
SphI G_CATG'C
Cuts at: 0 18 2232
Size: 18 2214
StuI AGG'CCT
Cuts at: 0 385 2232
Size: 385 1847
StyI C'CwwG_G
Cuts at: 0 461 602 2232
Size: 461 141 1630
Fragments arranged by size:
1630 461 141
TatI w'GTAC_w
Cuts at: 0 1378 2232
Size: 1378 854
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 933 965 2232
Size: 933 32 1267
Fragments arranged by size:
1267 933 32
XbaI T'CTAG_A
Cuts at: 0 666 1551 2232
Size: 666 885 681
Fragments arranged by size:
885 681 666
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1383 2232
Size: 1383 849
XhoI C'TCGA_G
Cuts at: 0 1544 2232
Size: 1544 688
XmnI GAAnn'nnTTC
Cuts at: 0 362 2232
Size: 362 1870
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApoI AvaI BamHI
BanI BanII BbsI BcgI BglII Bme1580I BmrI BmtI
BplI BpmI BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsgI BsmI Bsp1286I BspHI BsrDI BstAPI BstEII BstYI
Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NheI NspI PciI PflMI
PmlI PpuMI PspXI PstI PvuII SapI ScaI SfcI
SmlI SphI StuI StyI TatI TstI XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BglI BlpI
BmgBI Bpu10I BpuEI BsaHI BsaWI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoRI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I
XmaI ZraI