Restriction map for
pF1KB4886
(Linear) (Six-base) MAPSORT of: KIBB4886.seq Check: 2046 from: 1 to: 1020
KIBB4886.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
Acc65I G'GTAC_C
Cuts at: 0 431 1020
Size: 431 589
AleI CACnn'nnGTG
Cuts at: 0 595 1020
Size: 595 425
AvaI C'yCGr_G
Cuts at: 0 952 1020
Size: 952 68
BanI G'GyrC_C
Cuts at: 0 158 431 604 1020
Size: 158 273 173 416
Fragments arranged by size:
416 273 173 158
BanII G_rGCy'C
Cuts at: 0 782 991 1020
Size: 782 209 29
Fragments arranged by size:
782 209 29
BbeI G_GCGC'C
Cuts at: 0 608 1020
Size: 608 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 670 704 1020
Size: 670 34 316
Fragments arranged by size:
670 316 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 480 1020
Size: 480 540
BglII A'GATC_T
Cuts at: 0 170 1010 1020
Size: 170 840 10
Fragments arranged by size:
840 170 10
Bme1580I G_kGCm'C
Cuts at: 0 161 501 1020
Size: 161 340 519
Fragments arranged by size:
519 340 161
BmgBI CAC'GTC
Cuts at: 0 636 1020
Size: 636 384
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 195 437 1020
Size: 109 86 242 583
Fragments arranged by size:
583 242 109 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1020
Size: 31 989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 361 991 1020
Size: 183 178 630 29
Fragments arranged by size:
630 183 178 29
BsaAI yAC'GTr
Cuts at: 0 746 1020
Size: 746 274
BsaHI Gr'CG_yC
Cuts at: 0 605 740 1020
Size: 605 135 280
Fragments arranged by size:
605 280 135
BsaWI w'CCGG_w
Cuts at: 0 41 1020
Size: 41 979
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 1020
Size: 346 30 644
Fragments arranged by size:
644 346 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 279 772 1020
Size: 279 493 248
Fragments arranged by size:
493 279 248
BseYI C'CCAG_C
Cuts at: 0 103 1020
Size: 103 917
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1020
Size: 932 88
BsiEI CG_ry'CG
Cuts at: 0 909 1020
Size: 909 111
BsiHKAI G_wGCw'C
Cuts at: 0 782 1020
Size: 782 238
Bsp1286I G_dGCh'C
Cuts at: 0 161 501 782 991 1020
Size: 161 340 281 209 29
Fragments arranged by size:
340 281 209 161 29
BspEI T'CCGG_A
Cuts at: 0 41 1020
Size: 41 979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 770 1020
Size: 770 250
BsrFI r'CCGG_y
Cuts at: 0 727 1020
Size: 727 293
BstEII G'GTnAC_C
Cuts at: 0 957 1020
Size: 957 63
BstYI r'GATC_y
Cuts at: 0 50 170 1010 1020
Size: 50 120 840 10
Fragments arranged by size:
840 120 50 10
Bsu36I CC'TnA_GG
Cuts at: 0 321 1020
Size: 321 699
BtgI C'CryG_G
Cuts at: 0 153 983 1020
Size: 153 830 37
Fragments arranged by size:
830 153 37
BtsI GCAGTG_nn'
Cuts at: 0 698 1020
Size: 698 322
EaeI y'GGCC_r
Cuts at: 0 420 585 669 927 980 1020
Size: 420 165 84 258 53 40
Fragments arranged by size:
420 258 165 84 53 40
EarI CTCTTCn'nnn_
Cuts at: 0 760 1020
Size: 760 260
EcoICRI GAG'CTC
Cuts at: 0 780 1020
Size: 780 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1020
Size: 31 989
EcoO109I rG'GnC_Cy
Cuts at: 0 137 146 1020
Size: 137 9 874
Fragments arranged by size:
874 137 9
HaeII r_GCGC'y
Cuts at: 0 218 608 1020
Size: 218 390 412
Fragments arranged by size:
412 390 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 1020
Size: 547 32 441
Fragments arranged by size:
547 441 32
KasI G'GCGC_C
Cuts at: 0 604 1020
Size: 604 416
KpnI G_GTAC'C
Cuts at: 0 435 1020
Size: 435 585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 704 1020
Size: 329 375 316
Fragments arranged by size:
375 329 316
MscI TGG'CCA
Cuts at: 0 422 929 982 1020
Size: 422 507 53 38
Fragments arranged by size:
507 422 53 38
MslI CAynn'nnrTG
Cuts at: 0 595 976 1020
Size: 595 381 44
Fragments arranged by size:
595 381 44
MspA1I CmG'CkG
Cuts at: 0 103 282 842 1020
Size: 103 179 560 178
Fragments arranged by size:
560 179 178 103
NarI GG'CG_CC
Cuts at: 0 605 1020
Size: 605 415
NspI r_CATG'y
Cuts at: 0 74 1020
Size: 74 946
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 1020
Size: 185 835
PfoI T'CCnGG_A
Cuts at: 0 53 452 1020
Size: 53 399 568
Fragments arranged by size:
568 399 53
PmlI CAC'GTG
Cuts at: 0 746 1020
Size: 746 274
PpuMI rG'GwC_Cy
Cuts at: 0 137 146 1020
Size: 137 9 874
Fragments arranged by size:
874 137 9
PvuII CAG'CTG
Cuts at: 0 103 1020
Size: 103 917
SacI G_AGCT'C
Cuts at: 0 782 1020
Size: 782 238
SfcI C'TryA_G
Cuts at: 0 369 1020
Size: 369 651
SfoI GGC'GCC
Cuts at: 0 606 1020
Size: 606 414
SmlI C'TyrA_G
Cuts at: 0 198 376 1006 1020
Size: 198 178 630 14
Fragments arranged by size:
630 198 178 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 1020
Size: 208 812
TatI w'GTAC_w
Cuts at: 0 788 1020
Size: 788 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 634 666 1020
Size: 138 32 464 32 354
Fragments arranged by size:
464 354 138 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 100 1020
Size: 100 920
Enzymes that do cut:
Acc65I AleI AvaI BanI BanII BbeI BcgI BciVI
BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrFI BstEII BstYI Bsu36I BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NarI NspI PflMI
PfoI PmlI PpuMI PvuII SacI SfcI SfoI SmlI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BclI BfrBI BglI BlpI BmtI BplI Bpu10I BsaI
BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI