Restriction map for
pF1KB4878
(Linear) (Six-base) MAPSORT of: KIBB4878.seq Check: 3045 from: 1 to: 1533
KIBB4878.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AleI CACnn'nnGTG
Cuts at: 0 562 952 1533
Size: 562 390 581
Fragments arranged by size:
581 562 390
AlwNI CAG_nnn'CTG
Cuts at: 0 1242 1533
Size: 1242 291
ApoI r'AATT_y
Cuts at: 0 552 616 1533
Size: 552 64 917
Fragments arranged by size:
917 552 64
BanI G'GyrC_C
Cuts at: 0 1298 1339 1533
Size: 1298 41 194
Fragments arranged by size:
1298 194 41
BanII G_rGCy'C
Cuts at: 0 764 932 1533
Size: 764 168 601
Fragments arranged by size:
764 601 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 675 805 1365 1533
Size: 675 130 560 168
Fragments arranged by size:
675 560 168 130
BbvCI CC'TCA_GC
Cuts at: 0 547 1533
Size: 547 986
BclI T'GATC_A
Cuts at: 0 1310 1533
Size: 1310 223
BlpI GC'TnA_GC
Cuts at: 0 75 1533
Size: 75 1458
Bme1580I G_kGCm'C
Cuts at: 0 1301 1533
Size: 1301 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 1304 1533
Size: 1304 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1533
Size: 514 1019
Bpu10I CC'TnA_GC
Cuts at: 0 547 1533
Size: 547 986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1281 1533
Size: 1281 252
BsaI GGTCTCn'nnnn_
Cuts at: 0 652 1533
Size: 652 881
BsaWI w'CCGG_w
Cuts at: 0 686 1533
Size: 686 847
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 1533
Size: 181 1352
BseYI C'CCAG_C
Cuts at: 0 135 244 569 1326 1533
Size: 135 109 325 757 207
Fragments arranged by size:
757 325 207 135 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1533
Size: 535 998
BsiHKAI G_wGCw'C
Cuts at: 0 722 764 776 1533
Size: 722 42 12 757
Fragments arranged by size:
757 722 42 12
Bsp1286I G_dGCh'C
Cuts at: 0 722 764 776 932 1301 1533
Size: 722 42 12 156 369 232
Fragments arranged by size:
722 369 232 156 42 12
BspEI T'CCGG_A
Cuts at: 0 686 1533
Size: 686 847
BspHI T'CATG_A
Cuts at: 0 188 1533
Size: 188 1345
BsrBI CCG'CTC
Cuts at: 0 24 1533
Size: 24 1509
BsrDI GCAATG_nn'
Cuts at: 0 65 1533
Size: 65 1468
BsrFI r'CCGG_y
Cuts at: 0 750 1533
Size: 750 783
BstEII G'GTnAC_C
Cuts at: 0 564 1533
Size: 564 969
BstXI CCAn_nnnn'nTGG
Cuts at: 0 346 891 918 954 1287 1533
Size: 346 545 27 36 333 246
Fragments arranged by size:
545 346 333 246 36 27
BstYI r'GATC_y
Cuts at: 0 880 1533
Size: 880 653
Bsu36I CC'TnA_GG
Cuts at: 0 1405 1533
Size: 1405 128
BtgI C'CryG_G
Cuts at: 0 923 947 1149 1304 1533
Size: 923 24 202 155 229
Fragments arranged by size:
923 229 202 155 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 885 1533
Size: 885 648
BtsI GCAGTG_nn'
Cuts at: 0 396 1533
Size: 396 1137
EaeI y'GGCC_r
Cuts at: 0 25 173 920 990 1146 1289 1424 1533
Size: 25 148 747 70 156 143 135 109
Fragments arranged by size:
747 156 148 143 135 109 70 25
EarI CTCTTCn'nnn_
Cuts at: 0 779 1489 1533
Size: 779 710 44
Fragments arranged by size:
779 710 44
EcoICRI GAG'CTC
Cuts at: 0 762 1533
Size: 762 771
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1533
Size: 514 1019
EcoO109I rG'GnC_Cy
Cuts at: 0 227 1409 1533
Size: 227 1182 124
Fragments arranged by size:
1182 227 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 665 697 865 897 1533
Size: 1 32 632 32 168 32 636
Fragments arranged by size:
636 632 168 32 32 32 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1436 1533
Size: 1436 97
MscI TGG'CCA
Cuts at: 0 175 922 992 1291 1426 1533
Size: 175 747 70 299 135 107
Fragments arranged by size:
747 299 175 135 107 70
MslI CAynn'nnrTG
Cuts at: 0 338 562 916 952 1285 1533
Size: 338 224 354 36 333 248
Fragments arranged by size:
354 338 333 248 224 36
MspA1I CmG'CkG
Cuts at: 0 1422 1533
Size: 1422 111
NcoI C'CATG_G
Cuts at: 0 923 1149 1304 1533
Size: 923 226 155 229
Fragments arranged by size:
923 229 226 155
NspI r_CATG'y
Cuts at: 0 252 696 1533
Size: 252 444 837
Fragments arranged by size:
837 444 252
PfoI T'CCnGG_A
Cuts at: 0 1192 1533
Size: 1192 341
PpuMI rG'GwC_Cy
Cuts at: 0 227 1409 1533
Size: 227 1182 124
Fragments arranged by size:
1182 227 124
PvuII CAG'CTG
Cuts at: 0 1422 1533
Size: 1422 111
SacI G_AGCT'C
Cuts at: 0 764 1533
Size: 764 769
SexAI A'CCwGG_T
Cuts at: 0 968 1235 1533
Size: 968 267 298
Fragments arranged by size:
968 298 267
SfcI C'TryA_G
Cuts at: 0 387 1274 1533
Size: 387 887 259
Fragments arranged by size:
887 387 259
SmlI C'TyrA_G
Cuts at: 0 1260 1533
Size: 1260 273
SphI G_CATG'C
Cuts at: 0 252 696 1533
Size: 252 444 837
Fragments arranged by size:
837 444 252
SspI AAT'ATT
Cuts at: 0 300 1533
Size: 300 1233
StuI AGG'CCT
Cuts at: 0 393 1533
Size: 393 1140
StyI C'CwwG_G
Cuts at: 0 161 418 923 1034 1149 1304 1533
Size: 161 257 505 111 115 155 229
Fragments arranged by size:
505 257 229 161 155 115 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 464 1533
Size: 258 206 1069
Fragments arranged by size:
1069 258 206
TatI w'GTAC_w
Cuts at: 0 1442 1533
Size: 1442 91
Tth111I GACn'n_nGTC
Cuts at: 0 679 1533
Size: 679 854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 1533
Size: 168 1365
Enzymes that do cut:
AleI AlwNI ApoI BanI BanII BbsI BbvCI BclI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I MmeI
MscI MslI MspA1I NcoI NspI PfoI PpuMI PvuII
SacI SexAI SfcI SmlI SphI SspI StuI StyI
TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI
BciVI BfrBI BglI BglII BmgBI BmtI BplI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspMI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI TstI XbaI XhoI XmaI XmnI ZraI