Restriction map for
pF1KB4846
(Linear) (Six-base) MAPSORT of: KIBB4846.seq Check: 3164 from: 1 to: 1440
KIBB4846.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AatII G_ACGT'C
Cuts at: 0 92 1440
Size: 92 1348
AccI GT'mk_AC
Cuts at: 0 957 1440
Size: 957 483
AfeI AGC'GCT
Cuts at: 0 1267 1440
Size: 1267 173
ApaI G_GGCC'C
Cuts at: 0 599 1440
Size: 599 841
ApaLI G'TGCA_C
Cuts at: 0 886 1440
Size: 886 554
AvaI C'yCGr_G
Cuts at: 0 255 599 646 1083 1440
Size: 255 344 47 437 357
Fragments arranged by size:
437 357 344 255 47
AvrII C'CTAG_G
Cuts at: 0 360 1440
Size: 360 1080
BamHI G'GATC_C
Cuts at: 0 865 1440
Size: 865 575
BanI G'GyrC_C
Cuts at: 0 175 969 1051 1222 1398 1440
Size: 175 794 82 171 176 42
Fragments arranged by size:
794 176 175 171 82 42
BanII G_rGCy'C
Cuts at: 0 145 599 1117 1440
Size: 145 454 518 323
Fragments arranged by size:
518 454 323 145
BbeI G_GCGC'C
Cuts at: 0 179 1055 1226 1402 1440
Size: 179 876 171 176 38
Fragments arranged by size:
876 179 176 171 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 528 618 1440
Size: 528 90 822
Fragments arranged by size:
822 528 90
BbvCI CC'TCA_GC
Cuts at: 0 211 782 1440
Size: 211 571 658
Fragments arranged by size:
658 571 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1077 1111 1440
Size: 1077 34 329
Fragments arranged by size:
1077 329 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 798 1440
Size: 798 642
BclI T'GATC_A
Cuts at: 0 633 1440
Size: 633 807
BfrBI ATG'CAT
Cuts at: 0 62 1440
Size: 62 1378
BlpI GC'TnA_GC
Cuts at: 0 139 425 1440
Size: 139 286 1015
Fragments arranged by size:
1015 286 139
Bme1580I G_kGCm'C
Cuts at: 0 599 890 1440
Size: 599 291 550
Fragments arranged by size:
599 550 291
BmgBI CAC'GTC
Cuts at: 0 916 1353 1440
Size: 916 437 87
Fragments arranged by size:
916 437 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1435 1440
Size: 703 732 5
Fragments arranged by size:
732 703 5
Bpu10I CC'TnA_GC
Cuts at: 0 211 782 1440
Size: 211 571 658
Fragments arranged by size:
658 571 211
BsaHI Gr'CG_yC
Cuts at: 0 89 163 176 185 1052 1072 1223 1399
Size: 89 74 13 9 867 20 151 176
Cuts at: 1399 1440
Size: 41
Fragments arranged by size:
867 176 151 89 74 41 20 13
9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1231 1261 1366 1396 1411 1440
Size: 1231 30 105 30 15 29
Fragments arranged by size:
1231 105 30 30 29 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 1432 1440
Size: 228 1204 8
Fragments arranged by size:
1204 228 8
BseYI C'CCAG_C
Cuts at: 0 171 726 891 1330 1440
Size: 171 555 165 439 110
Fragments arranged by size:
555 439 171 165 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1278 1440
Size: 1278 162
BsiEI CG_ry'CG
Cuts at: 0 225 495 1232 1440
Size: 225 270 737 208
Fragments arranged by size:
737 270 225 208
BsiHKAI G_wGCw'C
Cuts at: 0 890 1440
Size: 890 550
BsmI GAATG_Cn'
Cuts at: 0 64 1440
Size: 64 1376
BsmBI CGTCTCn'nnnn_
Cuts at: 0 80 1440
Size: 80 1360
Bsp1286I G_dGCh'C
Cuts at: 0 145 599 890 1117 1440
Size: 145 454 291 227 323
Fragments arranged by size:
454 323 291 227 145
BspHI T'CATG_A
Cuts at: 0 50 1440
Size: 50 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1148 1440
Size: 1148 292
BsrBI CCG'CTC
Cuts at: 0 218 669 1440
Size: 218 451 771
Fragments arranged by size:
771 451 218
BsrDI GCAATG_nn'
Cuts at: 0 411 869 1440
Size: 411 458 571
Fragments arranged by size:
571 458 411
BsrFI r'CCGG_y
Cuts at: 0 187 202 1074 1231 1309 1440
Size: 187 15 872 157 78 131
Fragments arranged by size:
872 187 157 131 78 15
BssHII G'CGCG_C
Cuts at: 0 1408 1440
Size: 1408 32
BssSI C'ACGA_G
Cuts at: 0 105 746 1440
Size: 105 641 694
Fragments arranged by size:
694 641 105
BstEII G'GTnAC_C
Cuts at: 0 1077 1440
Size: 1077 363
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1395 1440
Size: 1395 45
BstYI r'GATC_y
Cuts at: 0 815 865 1440
Size: 815 50 575
Fragments arranged by size:
815 575 50
Bsu36I CC'TnA_GG
Cuts at: 0 481 1440
Size: 481 959
BtgI C'CryG_G
Cuts at: 0 832 1109 1250 1440
Size: 832 277 141 190
Fragments arranged by size:
832 277 190 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 324 1440
Size: 324 1116
BtsI GCAGTG_nn'
Cuts at: 0 767 1440
Size: 767 673
EaeI y'GGCC_r
Cuts at: 0 200 245 1106 1118 1229 1440
Size: 200 45 861 12 111 211
Fragments arranged by size:
861 211 200 111 45 12
EagI C'GGCC_G
Cuts at: 0 1229 1440
Size: 1229 211
EarI CTCTTCn'nnn_
Cuts at: 0 498 1440
Size: 498 942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1435 1440
Size: 703 732 5
Fragments arranged by size:
732 703 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1417 1440
Size: 1417 23
EcoO109I rG'GnC_Cy
Cuts at: 0 596 1433 1440
Size: 596 837 7
Fragments arranged by size:
837 596 7
FspI TGC'GCA
Cuts at: 0 271 1440
Size: 271 1169
FspAI rTGC'GCAy
Cuts at: 0 271 1440
Size: 271 1169
HaeII r_GCGC'y
Cuts at: 0 179 194 321 1055 1226 1269 1402 1440
Size: 179 15 127 734 171 43 133 38
Fragments arranged by size:
734 179 171 133 127 43 38 15
HincII GTy'rAC
Cuts at: 0 958 1440
Size: 958 482
HindIII A'AGCT_T
Cuts at: 0 513 1440
Size: 513 927
KasI G'GCGC_C
Cuts at: 0 175 1051 1222 1398 1440
Size: 175 876 171 176 42
Fragments arranged by size:
876 176 175 171 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1052 1440
Size: 843 209 388
Fragments arranged by size:
843 388 209
MslI CAynn'nnrTG
Cuts at: 0 394 703 1099 1393 1440
Size: 394 309 396 294 47
Fragments arranged by size:
396 394 309 294 47
MspA1I CmG'CkG
Cuts at: 0 171 690 923 1440
Size: 171 519 233 517
Fragments arranged by size:
519 517 233 171
NaeI GCC'GGC
Cuts at: 0 189 204 1233 1440
Size: 189 15 1029 207
Fragments arranged by size:
1029 207 189 15
NarI GG'CG_CC
Cuts at: 0 176 1052 1223 1399 1440
Size: 176 876 171 176 41
Fragments arranged by size:
876 176 176 171 41
NcoI C'CATG_G
Cuts at: 0 1109 1250 1440
Size: 1109 141 190
Fragments arranged by size:
1109 190 141
NgoMIV G'CCGG_C
Cuts at: 0 187 202 1231 1440
Size: 187 15 1029 209
Fragments arranged by size:
1029 209 187 15
NsiI A_TGCA'T
Cuts at: 0 64 1440
Size: 64 1376
NspI r_CATG'y
Cuts at: 0 1041 1104 1440
Size: 1041 63 336
Fragments arranged by size:
1041 336 63
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 1440
Size: 861 579
PshAI GACnn'nnGTC
Cuts at: 0 1075 1440
Size: 1075 365
PspOMI G'GGCC_C
Cuts at: 0 595 1440
Size: 595 845
PstI C_TGCA'G
Cuts at: 0 945 1440
Size: 945 495
PvuII CAG'CTG
Cuts at: 0 690 1440
Size: 690 750
SalI G'TCGA_C
Cuts at: 0 956 1440
Size: 956 484
SexAI A'CCwGG_T
Cuts at: 0 458 1440
Size: 458 982
SfcI C'TryA_G
Cuts at: 0 941 1440
Size: 941 499
SfoI GGC'GCC
Cuts at: 0 177 1053 1224 1400 1440
Size: 177 876 171 176 40
Fragments arranged by size:
876 177 176 171 40
SgrAI Cr'CCGG_yG
Cuts at: 0 187 1440
Size: 187 1253
SmaI CCC'GGG
Cuts at: 0 257 648 1440
Size: 257 391 792
Fragments arranged by size:
792 391 257
SphI G_CATG'C
Cuts at: 0 1041 1440
Size: 1041 399
StyI C'CwwG_G
Cuts at: 0 360 1109 1250 1440
Size: 360 749 141 190
Fragments arranged by size:
749 360 190 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 740 905 1440
Size: 481 259 165 535
Fragments arranged by size:
535 481 259 165
TatI w'GTAC_w
Cuts at: 0 1214 1440
Size: 1214 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 1411 1440
Size: 1366 32 13 29
Fragments arranged by size:
1366 32 29 13
XbaI T'CTAG_A
Cuts at: 0 82 1440
Size: 82 1358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 830 1440
Size: 830 610
XmaI C'CCGG_G
Cuts at: 0 255 646 1440
Size: 255 391 794
Fragments arranged by size:
794 391 255
XmnI GAAnn'nnTTC
Cuts at: 0 514 1440
Size: 514 926
ZraI GAC'GTC
Cuts at: 0 90 1440
Size: 90 1350
Enzymes that do cut:
AatII AccI AfeI ApaI ApaLI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI Eco57MI EcoNI EcoO109I FspI FspAI HaeII HincII
HindIII KasI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PflMI PshAI PspOMI PstI PvuII
SalI SexAI SfcI SfoI SgrAI SmaI SphI StyI
TaqII TatI TstI XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI
BaeI BglI BglII BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsiWI BspEI BsrGI BstAPI BstBI
BstZ17I ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoRI EcoRV FalI FseI Hin4I HpaI KpnI MfeI
MluI MscI NdeI NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SfiI SgfI SmlI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XhoI