Restriction map for
pF1KB4800
(Linear) (Six-base) MAPSORT of: KIBB4800.seq Check: 4891 from: 1 to: 1266
KIBB4800.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 998 1266
Size: 998 268
Acc65I G'GTAC_C
Cuts at: 0 838 1266
Size: 838 428
AhdI GACnn_n'nnGTC
Cuts at: 0 162 609 1266
Size: 162 447 657
Fragments arranged by size:
657 447 162
AleI CACnn'nnGTG
Cuts at: 0 1088 1266
Size: 1088 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 757 1266
Size: 757 509
AlwNI CAG_nnn'CTG
Cuts at: 0 236 1266
Size: 236 1030
ApaI G_GGCC'C
Cuts at: 0 432 1266
Size: 432 834
ApaLI G'TGCA_C
Cuts at: 0 732 814 913 1266
Size: 732 82 99 353
Fragments arranged by size:
732 353 99 82
AvaI C'yCGr_G
Cuts at: 0 152 667 808 1266
Size: 152 515 141 458
Fragments arranged by size:
515 458 152 141
AvrII C'CTAG_G
Cuts at: 0 588 1266
Size: 588 678
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 822 855 1266
Size: 822 33 411
Fragments arranged by size:
822 411 33
BanI G'GyrC_C
Cuts at: 0 99 398 540 568 838 1238 1266
Size: 99 299 142 28 270 400 28
Fragments arranged by size:
400 299 270 142 99 28 28
BanII G_rGCy'C
Cuts at: 0 152 250 432 596 809 1141 1262 1266
Size: 152 98 182 164 213 332 121 4
Fragments arranged by size:
332 213 182 164 152 121 98 4
BbeI G_GCGC'C
Cuts at: 0 103 544 1266
Size: 103 441 722
Fragments arranged by size:
722 441 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 858 892 1266
Size: 858 34 374
Fragments arranged by size:
858 374 34
BglI GCCn_nnn'nGGC
Cuts at: 0 804 1266
Size: 804 462
BglII A'GATC_T
Cuts at: 0 944 1266
Size: 944 322
Bme1580I G_kGCm'C
Cuts at: 0 319 432 736 818 884 917 1266
Size: 319 113 304 82 66 33 349
Fragments arranged by size:
349 319 304 113 82 66 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 288 454 916 1266
Size: 278 10 166 462 350
Fragments arranged by size:
462 350 278 166 10
BsaAI yAC'GTr
Cuts at: 0 819 1266
Size: 819 447
BsaHI Gr'CG_yC
Cuts at: 0 100 541 707 1266
Size: 100 441 166 559
Fragments arranged by size:
559 441 166 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 715 745 1266
Size: 187 30 498 30 521
Fragments arranged by size:
521 498 187 30 30
BseYI C'CCAG_C
Cuts at: 0 335 747 1266
Size: 335 412 519
Fragments arranged by size:
519 412 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 670 798 1266
Size: 427 243 128 468
Fragments arranged by size:
468 427 243 128
BsiEI CG_ry'CG
Cuts at: 0 421 494 1266
Size: 421 73 772
Fragments arranged by size:
772 421 73
BsiHKAI G_wGCw'C
Cuts at: 0 250 736 818 917 1141 1266
Size: 250 486 82 99 224 125
Fragments arranged by size:
486 250 224 125 99 82
BsmI GAATG_Cn'
Cuts at: 0 1126 1266
Size: 1126 140
BsmBI CGTCTCn'nnnn_
Cuts at: 0 296 1240 1266
Size: 296 944 26
Fragments arranged by size:
944 296 26
Bsp1286I G_dGCh'C
Cuts at: 0 152 250 319 432 596 736 809 818
Size: 152 98 69 113 164 140 73 9
Cuts at: 818 884 917 1141 1262 1266
Size: 66 33 224 121 4
Fragments arranged by size:
224 164 152 140 121 113 98 73
69 66 33 9 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 815 998 1266
Size: 401 414 183 268
Fragments arranged by size:
414 401 268 183
BsrBI CCG'CTC
Cuts at: 0 374 1266
Size: 374 892
BsrFI r'CCGG_y
Cuts at: 0 96 520 565 1266
Size: 96 424 45 701
Fragments arranged by size:
701 424 96 45
BstAPI GCAn_nnn'nTGC
Cuts at: 0 236 405 776 1266
Size: 236 169 371 490
Fragments arranged by size:
490 371 236 169
BstYI r'GATC_y
Cuts at: 0 944 1266
Size: 944 322
BtgI C'CryG_G
Cuts at: 0 641 678 1266
Size: 641 37 588
Fragments arranged by size:
641 588 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 305 1266
Size: 305 961
DraIII CAC_nnn'GTG
Cuts at: 0 405 888 1266
Size: 405 483 378
Fragments arranged by size:
483 405 378
EaeI y'GGCC_r
Cuts at: 0 483 491 675 1266
Size: 483 8 184 591
Fragments arranged by size:
591 483 184 8
EagI C'GGCC_G
Cuts at: 0 491 1266
Size: 491 775
EarI CTCTTCn'nnn_
Cuts at: 0 463 949 1266
Size: 463 486 317
Fragments arranged by size:
486 463 317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 160 1266
Size: 154 6 1106
Fragments arranged by size:
1106 154 6
EcoICRI GAG'CTC
Cuts at: 0 248 1139 1266
Size: 248 891 127
Fragments arranged by size:
891 248 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 288 454 916 1266
Size: 278 10 166 462 350
Fragments arranged by size:
462 350 278 166 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 587 1266
Size: 587 679
EcoO109I rG'GnC_Cy
Cuts at: 0 272 428 429 827 1266
Size: 272 156 1 398 439
Fragments arranged by size:
439 398 272 156 1
FspI TGC'GCA
Cuts at: 0 823 1266
Size: 823 443
HaeII r_GCGC'y
Cuts at: 0 103 446 544 704 1266
Size: 103 343 98 160 562
Fragments arranged by size:
562 343 160 103 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 187 219 595 627 1266
Size: 148 32 7 32 376 32 639
Fragments arranged by size:
639 376 148 32 32 32 7
KasI G'GCGC_C
Cuts at: 0 99 540 1266
Size: 99 441 726
Fragments arranged by size:
726 441 99
KpnI G_GTAC'C
Cuts at: 0 842 1266
Size: 842 424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1166 1266
Size: 701 465 100
Fragments arranged by size:
701 465 100
MscI TGG'CCA
Cuts at: 0 485 677 1266
Size: 485 192 589
Fragments arranged by size:
589 485 192
MslI CAynn'nnrTG
Cuts at: 0 1088 1266
Size: 1088 178
MspA1I CmG'CkG
Cuts at: 0 180 221 577 649 836 1266
Size: 180 41 356 72 187 430
Fragments arranged by size:
430 356 187 180 72 41
NaeI GCC'GGC
Cuts at: 0 522 1266
Size: 522 744
NarI GG'CG_CC
Cuts at: 0 100 541 1266
Size: 100 441 725
Fragments arranged by size:
725 441 100
NgoMIV G'CCGG_C
Cuts at: 0 520 1266
Size: 520 746
PmlI CAC'GTG
Cuts at: 0 819 1266
Size: 819 447
PpuMI rG'GwC_Cy
Cuts at: 0 827 1266
Size: 827 439
PspOMI G'GGCC_C
Cuts at: 0 428 1266
Size: 428 838
PspXI vC'TCGA_Gb
Cuts at: 0 808 1266
Size: 808 458
PstI C_TGCA'G
Cuts at: 0 556 1266
Size: 556 710
PvuII CAG'CTG
Cuts at: 0 180 221 1266
Size: 180 41 1045
Fragments arranged by size:
1045 180 41
SacI G_AGCT'C
Cuts at: 0 250 1141 1266
Size: 250 891 125
Fragments arranged by size:
891 250 125
SapI GCTCTTCn'nnn_
Cuts at: 0 949 1266
Size: 949 317
ScaI AGT'ACT
Cuts at: 0 1030 1266
Size: 1030 236
SexAI A'CCwGG_T
Cuts at: 0 401 1266
Size: 401 865
SfcI C'TryA_G
Cuts at: 0 552 1266
Size: 552 714
SfoI GGC'GCC
Cuts at: 0 101 542 1266
Size: 101 441 724
Fragments arranged by size:
724 441 101
SmaI CCC'GGG
Cuts at: 0 154 1266
Size: 154 1112
SmlI C'TyrA_G
Cuts at: 0 808 1266
Size: 808 458
StyI C'CwwG_G
Cuts at: 0 588 1266
Size: 588 678
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 1168 1266
Size: 199 969 98
Fragments arranged by size:
969 199 98
TatI w'GTAC_w
Cuts at: 0 1028 1266
Size: 1028 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 715 747 1266
Size: 715 32 519
Fragments arranged by size:
715 519 32
XhoI C'TCGA_G
Cuts at: 0 808 1266
Size: 808 458
XmaI C'CCGG_G
Cuts at: 0 152 1266
Size: 152 1114
XmnI GAAnn'nnTTC
Cuts at: 0 958 1210 1266
Size: 958 252 56
Fragments arranged by size:
958 252 56
Enzymes that do cut:
AarI Acc65I AhdI AleI AlfI AlwNI ApaI ApaLI
AvaI AvrII BaeI BanI BanII BbeI BcgI BglI
BglII Bme1580I BpmI BsaAI BsaHI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstYI BtgI BtgZI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV PmlI PpuMI PspOMI PspXI PstI PvuII SacI
SapI ScaI SexAI SfcI SfoI SmaI SmlI StyI
TaqII TatI TstI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AloI ApoI AscI AseI AsiSI BamHI BbsI BbvCI
BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BsiWI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SacII SalI SanDI SbfI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI ZraI