Restriction map for
pF1KB4712
(Linear) (Six-base) MAPSORT of: KIBB4712.seq Check: 8345 from: 1 to: 741
KIBB4712.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AclI AA'CG_TT
Cuts at: 0 152 741
Size: 152 589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 425 741
Size: 391 34 316
Fragments arranged by size:
391 316 34
AfeI AGC'GCT
Cuts at: 0 55 741
Size: 55 686
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 534 741
Size: 211 323 207
Fragments arranged by size:
323 211 207
BclI T'GATC_A
Cuts at: 0 323 741
Size: 323 418
BglI GCCn_nnn'nGGC
Cuts at: 0 37 46 741
Size: 37 9 695
Fragments arranged by size:
695 37 9
BlpI GC'TnA_GC
Cuts at: 0 301 741
Size: 301 440
Bme1580I G_kGCm'C
Cuts at: 0 165 741
Size: 165 576
BmrI ACTGGGnnnn_n'
Cuts at: 0 339 741
Size: 339 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 741
Size: 198 543
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 741
Size: 334 407
BsaHI Gr'CG_yC
Cuts at: 0 608 741
Size: 608 133
BsaWI w'CCGG_w
Cuts at: 0 415 741
Size: 415 326
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 301 625 637 655 667 741
Size: 271 30 324 12 18 12 74
Fragments arranged by size:
324 271 74 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 741
Size: 664 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 741
Size: 43 698
BsiHKAI G_wGCw'C
Cuts at: 0 491 741
Size: 491 250
Bsp1286I G_dGCh'C
Cuts at: 0 165 491 741
Size: 165 326 250
Fragments arranged by size:
326 250 165
BsrBI CCG'CTC
Cuts at: 0 170 741
Size: 170 571
BsrDI GCAATG_nn'
Cuts at: 0 228 741
Size: 228 513
BssSI C'ACGA_G
Cuts at: 0 472 741
Size: 472 269
BstAPI GCAn_nnn'nTGC
Cuts at: 0 21 741
Size: 21 720
BstYI r'GATC_y
Cuts at: 0 554 741
Size: 554 187
BtgI C'CryG_G
Cuts at: 0 71 741
Size: 71 670
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 597 741
Size: 597 144
EaeI y'GGCC_r
Cuts at: 0 38 68 74 410 587 741
Size: 38 30 6 336 177 154
Fragments arranged by size:
336 177 154 38 30 6
EarI CTCTTCn'nnn_
Cuts at: 0 177 741
Size: 177 564
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 741
Size: 64 677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 391 425 741
Size: 198 193 34 316
Fragments arranged by size:
316 198 193 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 634 741
Size: 634 107
HaeII r_GCGC'y
Cuts at: 0 57 741
Size: 57 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 741
Size: 94 32 615
Fragments arranged by size:
615 94 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 741
Size: 257 484
MscI TGG'CCA
Cuts at: 0 412 589 741
Size: 412 177 152
Fragments arranged by size:
412 177 152
MspA1I CmG'CkG
Cuts at: 0 73 741
Size: 73 668
NspI r_CATG'y
Cuts at: 0 495 741
Size: 495 246
PfoI T'CCnGG_A
Cuts at: 0 175 741
Size: 175 566
PstI C_TGCA'G
Cuts at: 0 216 741
Size: 216 525
SacII CC_GC'GG
Cuts at: 0 74 741
Size: 74 667
SapI GCTCTTCn'nnn_
Cuts at: 0 177 741
Size: 177 564
SfcI C'TryA_G
Cuts at: 0 212 459 741
Size: 212 247 282
Fragments arranged by size:
282 247 212
StuI AGG'CCT
Cuts at: 0 457 521 741
Size: 457 64 220
Fragments arranged by size:
457 220 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 517 741
Size: 517 224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 741
Size: 271 32 438
Fragments arranged by size:
438 271 32
Enzymes that do cut:
AclI AcuI AfeI BbsI BclI BglI BlpI Bme1580I
BmrI BpmI BsaI BsaHI BsaWI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BsrBI BsrDI BssSI BstAPI BstYI BtgI
BtgZI EaeI EarI EciI Eco57MI EcoNI HaeII Hin4I
MmeI MscI MspA1I NspI PfoI PstI SacII SapI
SfcI StuI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI