Restriction map for
pF1KB4670
(Linear) (Six-base) MAPSORT of: KIBB4670.seq Check: 644 from: 1 to: 1203
KIBB4670.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 0 338 1203
Size: 338 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1203
Size: 1037 166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 1203
Size: 38 32 1133
Fragments arranged by size:
1133 38 32
ApaI G_GGCC'C
Cuts at: 0 704 1203
Size: 704 499
AseI AT'TA_AT
Cuts at: 0 983 1203
Size: 983 220
AvaI C'yCGr_G
Cuts at: 0 308 1203
Size: 308 895
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 889 922 1203
Size: 889 33 281
Fragments arranged by size:
889 281 33
BamHI G'GATC_C
Cuts at: 0 196 1133 1203
Size: 196 937 70
Fragments arranged by size:
937 196 70
BanI G'GyrC_C
Cuts at: 0 245 691 1203
Size: 245 446 512
Fragments arranged by size:
512 446 245
BanII G_rGCy'C
Cuts at: 0 704 1203
Size: 704 499
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 1203
Size: 366 837
Bme1580I G_kGCm'C
Cuts at: 0 248 704 1203
Size: 248 456 499
Fragments arranged by size:
499 456 248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 898 1203
Size: 898 305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 690 1203
Size: 338 352 513
Fragments arranged by size:
513 352 338
BsaI GGTCTCn'nnnn_
Cuts at: 0 68 650 1203
Size: 68 582 553
Fragments arranged by size:
582 553 68
BsaBI GATnn'nnATC
Cuts at: 0 803 1203
Size: 803 400
BsaHI Gr'CG_yC
Cuts at: 0 212 335 1203
Size: 212 123 868
Fragments arranged by size:
868 212 123
BsaWI w'CCGG_w
Cuts at: 0 807 1203
Size: 807 396
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 288 318 325 355 568 598
Size: 38 30 220 30 7 30 213 30
Cuts at: 598 1203
Size: 605
Fragments arranged by size:
605 220 213 38 30 30 30 30
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 333 606 1203
Size: 333 273 597
Fragments arranged by size:
597 333 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 1203
Size: 285 918
BsiEI CG_ry'CG
Cuts at: 0 94 1203
Size: 94 1109
BsmBI CGTCTCn'nnnn_
Cuts at: 0 326 569 1203
Size: 326 243 634
Fragments arranged by size:
634 326 243
Bsp1286I G_dGCh'C
Cuts at: 0 248 704 1203
Size: 248 456 499
Fragments arranged by size:
499 456 248
BspEI T'CCGG_A
Cuts at: 0 807 1203
Size: 807 396
BspHI T'CATG_A
Cuts at: 0 597 869 1203
Size: 597 272 334
Fragments arranged by size:
597 334 272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 307 375 1024 1203
Size: 307 68 649 179
Fragments arranged by size:
649 307 179 68
BsrBI CCG'CTC
Cuts at: 0 132 731 1203
Size: 132 599 472
Fragments arranged by size:
599 472 132
BsrDI GCAATG_nn'
Cuts at: 0 1195 1203
Size: 1195 8
BsrFI r'CCGG_y
Cuts at: 0 183 1203
Size: 183 1020
BssHII G'CGCG_C
Cuts at: 0 155 1203
Size: 155 1048
BstYI r'GATC_y
Cuts at: 0 196 1133 1203
Size: 196 937 70
Fragments arranged by size:
937 196 70
BtgI C'CryG_G
Cuts at: 0 88 120 1094 1203
Size: 88 32 974 109
Fragments arranged by size:
974 109 88 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 351 1203
Size: 351 852
BtsI GCAGTG_nn'
Cuts at: 0 750 1203
Size: 750 453
DrdI GACnn_nn'nnGTC
Cuts at: 0 370 1203
Size: 370 833
EaeI y'GGCC_r
Cuts at: 0 91 185 1203
Size: 91 94 1018
Fragments arranged by size:
1018 94 91
EagI C'GGCC_G
Cuts at: 0 91 1203
Size: 91 1112
EarI CTCTTCn'nnn_
Cuts at: 0 889 911 1203
Size: 889 22 292
Fragments arranged by size:
889 292 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 344 1203
Size: 344 859
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 690 1037 1203
Size: 338 352 347 166
Fragments arranged by size:
352 347 338 166
EcoNI CCTnn'n_nnAGG
Cuts at: 0 788 1203
Size: 788 415
EcoO109I rG'GnC_Cy
Cuts at: 0 700 701 1147 1203
Size: 700 1 446 56
Fragments arranged by size:
700 446 56 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 188 220 288 320 325 357 568 600
Size: 188 32 68 32 5 32 211 32
Cuts at: 600 658 690 1203
Size: 58 32 513
Fragments arranged by size:
513 211 188 68 58 32 32 32
32 32 5
HincII GTy'rAC
Cuts at: 0 207 1203
Size: 207 996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 1091 1203
Size: 371 720 112
Fragments arranged by size:
720 371 112
MslI CAynn'nnrTG
Cuts at: 0 173 665 1171 1203
Size: 173 492 506 32
Fragments arranged by size:
506 492 173 32
MspA1I CmG'CkG
Cuts at: 0 90 202 1203
Size: 90 112 1001
Fragments arranged by size:
1001 112 90
NaeI GCC'GGC
Cuts at: 0 185 1203
Size: 185 1018
NcoI C'CATG_G
Cuts at: 0 1094 1203
Size: 1094 109
NgoMIV G'CCGG_C
Cuts at: 0 183 1203
Size: 183 1020
PasI CC'CwG_GG
Cuts at: 0 697 1203
Size: 697 506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 1203
Size: 38 32 1133
Fragments arranged by size:
1133 38 32
PpuMI rG'GwC_Cy
Cuts at: 0 1147 1203
Size: 1147 56
PspOMI G'GGCC_C
Cuts at: 0 700 1203
Size: 700 503
PstI C_TGCA'G
Cuts at: 0 1146 1203
Size: 1146 57
SacII CC_GC'GG
Cuts at: 0 91 1203
Size: 91 1112
SapI GCTCTTCn'nnn_
Cuts at: 0 889 911 1203
Size: 889 22 292
Fragments arranged by size:
889 292 22
SbfI CC_TGCA'GG
Cuts at: 0 1146 1203
Size: 1146 57
ScaI AGT'ACT
Cuts at: 0 458 1203
Size: 458 745
SexAI A'CCwGG_T
Cuts at: 0 783 1203
Size: 783 420
SfcI C'TryA_G
Cuts at: 0 1142 1203
Size: 1142 61
SmaI CCC'GGG
Cuts at: 0 310 1203
Size: 310 893
StyI C'CwwG_G
Cuts at: 0 1062 1094 1203
Size: 1062 32 109
Fragments arranged by size:
1062 109 32
TatI w'GTAC_w
Cuts at: 0 456 1002 1203
Size: 456 546 201
Fragments arranged by size:
546 456 201
Tth111I GACn'n_nGTC
Cuts at: 0 559 1203
Size: 559 644
XmaI C'CCGG_G
Cuts at: 0 308 1203
Size: 308 895
ZraI GAC'GTC
Cuts at: 0 336 1203
Size: 336 867
Enzymes that do cut:
AatII AcuI AloI ApaI AseI AvaI BaeI BamHI
BanI BanII BbsI Bme1580I BplI BpmI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstYI
BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MslI MspA1I
NaeI NcoI NgoMIV PasI PpiI PpuMI PspOMI PstI
SacII SapI SbfI ScaI SexAI SfcI SmaI StyI
TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AsiSI
AvrII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI
BseYI BsiHKAI BsiWI BsmI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI XbaI XcmI XhoI XmnI