(Linear) (Six-base) MAPSORT of: KIBB4666.seq  Check: 5098  from: 1  to: 204



KIBB4666.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AfeI AGC'GCT

Cuts at:      0      34     204 
   Size:         34     170
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       9      43     204 
   Size:          9      34     161

  Fragments arranged by size: 

                 161      34       9
 
BmtI G_CTAG'C

Cuts at:      0      17     204 
   Size:         17     187
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     204 
   Size:         34     170
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      87     204 
   Size:         87     117
 
BsmI GAATG_Cn'

Cuts at:      0     129     204 
   Size:        129      75
 
BstYI r'GATC_y

Cuts at:      0     187     204 
   Size:        187      17
 
HaeII r_GCGC'y

Cuts at:      0      26      36     204 
   Size:         26      10     168

  Fragments arranged by size: 

                 168      26      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204 
   Size:        172      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     204 
   Size:         35     169
 
MspA1I CmG'CkG

Cuts at:      0      94     160     204 
   Size:         94      66      44

  Fragments arranged by size: 

                  94      66      44
 
NheI G'CTAG_C

Cuts at:      0      13     204 
   Size:         13     191
 
PfoI T'CCnGG_A

Cuts at:      0     176     204 
   Size:        176      28
 
PstI C_TGCA'G

Cuts at:      0      32      99     204 
   Size:         32      67     105

  Fragments arranged by size: 

                 105      67      32
 
PvuII CAG'CTG

Cuts at:      0      94     160     204 
   Size:         94      66      44

  Fragments arranged by size: 

                  94      66      44
 
ScaI AGT'ACT

Cuts at:      0     112     204 
   Size:        112      92
 
SfcI C'TryA_G

Cuts at:      0      28      95     204 
   Size:         28      67     109

  Fragments arranged by size: 

                 109      67      28
 
SmlI C'TyrA_G

Cuts at:      0      49     204 
   Size:         49     155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     139     204 
   Size:        139      65
 
TatI w'GTAC_w

Cuts at:      0     110     204 
   Size:        110      94
 

 Enzymes that do cut:

     AfeI     BcgI     BmtI    BpuEI     BsaI     BsmI    BstYI    HaeII
    Hin4I     MmeI   MspA1I     NheI     PfoI     PstI    PvuII     ScaI
     SfcI     SmlI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI