Restriction map for
pF1KB4626
(Linear) (Six-base) MAPSORT of: KIBB4626.seq Check: 119 from: 1 to: 2310
KIBB4626.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2223 2310
Size: 2223 87
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1594 2310
Size: 1333 261 716
Fragments arranged by size:
1333 716 261
AflIII A'CryG_T
Cuts at: 0 442 872 2310
Size: 442 430 1438
Fragments arranged by size:
1438 442 430
AleI CACnn'nnGTG
Cuts at: 0 940 1799 2310
Size: 940 859 511
Fragments arranged by size:
940 859 511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 97 215 536 976 1691 2310
Size: 97 118 321 440 715 619
Fragments arranged by size:
715 619 440 321 118 97
AlwNI CAG_nnn'CTG
Cuts at: 0 164 339 540 2001 2223 2310
Size: 164 175 201 1461 222 87
Fragments arranged by size:
1461 222 201 175 164 87
ApaI G_GGCC'C
Cuts at: 0 1251 1591 2310
Size: 1251 340 719
Fragments arranged by size:
1251 719 340
ApaLI G'TGCA_C
Cuts at: 0 109 190 349 1666 2310
Size: 109 81 159 1317 644
Fragments arranged by size:
1317 644 159 109 81
AvaI C'yCGr_G
Cuts at: 0 414 1037 1126 2294 2310
Size: 414 623 89 1168 16
Fragments arranged by size:
1168 623 414 89 16
BamHI G'GATC_C
Cuts at: 0 1691 2310
Size: 1691 619
BanI G'GyrC_C
Cuts at: 0 466 499 632 1228 1458 1506 2200 2310
Size: 466 33 133 596 230 48 694 110
Fragments arranged by size:
694 596 466 230 133 110 48 33
BanII G_rGCy'C
Cuts at: 0 677 1214 1251 1388 1591 1607 2128 2198
Size: 677 537 37 137 203 16 521 70
Cuts at: 2198 2310
Size: 112
Fragments arranged by size:
677 537 521 203 137 112 70 37
16
BbeI G_GCGC'C
Cuts at: 0 1232 1510 2204 2310
Size: 1232 278 694 106
Fragments arranged by size:
1232 694 278 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 1898 1975 2185 2310
Size: 1898 77 210 125
Fragments arranged by size:
1898 210 125 77
BbvCI CC'TCA_GC
Cuts at: 0 397 1713 2310
Size: 397 1316 597
Fragments arranged by size:
1316 597 397
BclI T'GATC_A
Cuts at: 0 1055 2310
Size: 1055 1255
BglI GCCn_nnn'nGGC
Cuts at: 0 625 1452 2310
Size: 625 827 858
Fragments arranged by size:
858 827 625
BglII A'GATC_T
Cuts at: 0 760 2310
Size: 760 1550
BlpI GC'TnA_GC
Cuts at: 0 838 2310
Size: 838 1472
Bme1580I G_kGCm'C
Cuts at: 0 113 194 353 471 502 635 965 1251
Size: 113 81 159 118 31 133 330 286
Cuts at: 1251 1463 1591 1670 1724 2045 2189 2310
Size: 212 128 79 54 321 144 121
Fragments arranged by size:
330 321 286 212 159 144 133 128
121 118 113 81 79 54 31
BmgBI CAC'GTC
Cuts at: 0 873 1671 2310
Size: 873 798 639
Fragments arranged by size:
873 798 639
BmrI ACTGGGnnnn_n'
Cuts at: 0 1472 2200 2310
Size: 1472 728 110
Fragments arranged by size:
1472 728 110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2100 2310
Size: 2100 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1327 1433 2131 2310
Size: 457 870 106 698 179
Fragments arranged by size:
870 698 457 179 106
Bpu10I CC'TnA_GC
Cuts at: 0 397 1105 1375 1713 2310
Size: 397 708 270 338 597
Fragments arranged by size:
708 597 397 338 270
BsaI GGTCTCn'nnnn_
Cuts at: 0 1919 2310
Size: 1919 391
BsaAI yAC'GTr
Cuts at: 0 516 2310
Size: 516 1794
BsaHI Gr'CG_yC
Cuts at: 0 126 1229 1507 1652 2201 2310
Size: 126 1103 278 145 549 109
Fragments arranged by size:
1103 549 278 145 126 109
BsaWI w'CCGG_w
Cuts at: 0 113 1388 1688 1694 2310
Size: 113 1275 300 6 616
Fragments arranged by size:
1275 616 300 113 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 1134 1164 1457 1487 2310
Size: 565 30 539 30 293 30 823
Fragments arranged by size:
823 565 539 293 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1254 1725 2310
Size: 1254 471 585
Fragments arranged by size:
1254 585 471
BseYI C'CCAG_C
Cuts at: 0 59 1848 1878 1945 2310
Size: 59 1789 30 67 365
Fragments arranged by size:
1789 365 67 59 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 496 1366 2310
Size: 277 219 870 944
Fragments arranged by size:
944 870 277 219
BsiEI CG_ry'CG
Cuts at: 0 267 2310
Size: 267 2043
BsiHKAI G_wGCw'C
Cuts at: 0 113 194 233 353 677 1388 1522 1607
Size: 113 81 39 120 324 711 134 85
Cuts at: 1607 1670 2128 2310
Size: 63 458 182
Fragments arranged by size:
711 458 324 182 134 120 113 85
81 63 39
Bsp1286I G_dGCh'C
Cuts at: 0 113 194 233 353 471 502 635 677
Size: 113 81 39 120 118 31 133 42
Cuts at: 677 965 1214 1251 1388 1463 1522 1591 1607
Size: 288 249 37 137 75 59 69 16
Cuts at: 1607 1670 1724 2045 2128 2189 2198 2310
Size: 63 54 321 83 61 9 112
Fragments arranged by size:
321 288 249 137 133 120 118 113
112 83 81 75 69 63 61 59
54 42 39 37 31 16 9
BspEI T'CCGG_A
Cuts at: 0 1388 1694 2310
Size: 1388 306 616
Fragments arranged by size:
1388 616 306
BspHI T'CATG_A
Cuts at: 0 1355 2310
Size: 1355 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1439 1968 2223 2310
Size: 1439 529 255 87
Fragments arranged by size:
1439 529 255 87
BsrBI CCG'CTC
Cuts at: 0 22 263 2310
Size: 22 241 2047
Fragments arranged by size:
2047 241 22
BsrFI r'CCGG_y
Cuts at: 0 266 683 845 1225 1455 2156 2310
Size: 266 417 162 380 230 701 154
Fragments arranged by size:
701 417 380 266 230 162 154
BssSI C'ACGA_G
Cuts at: 0 1776 2310
Size: 1776 534
BstAPI GCAn_nnn'nTGC
Cuts at: 0 189 237 2310
Size: 189 48 2073
Fragments arranged by size:
2073 189 48
BstEII G'GTnAC_C
Cuts at: 0 1980 2280 2310
Size: 1980 300 30
Fragments arranged by size:
1980 300 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1801 2277 2310
Size: 1801 476 33
Fragments arranged by size:
1801 476 33
BstYI r'GATC_y
Cuts at: 0 760 1406 1691 1932 2057 2310
Size: 760 646 285 241 125 253
Fragments arranged by size:
760 646 285 253 241 125
BtgI C'CryG_G
Cuts at: 0 866 1502 1794 2310
Size: 866 636 292 516
Fragments arranged by size:
866 636 516 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2241 2310
Size: 2241 69
BtsI GCAGTG_nn'
Cuts at: 0 538 914 960 1806 1843 2128 2310
Size: 538 376 46 846 37 285 182
Fragments arranged by size:
846 538 376 285 182 46 37
EaeI y'GGCC_r
Cuts at: 0 264 597 806 2310
Size: 264 333 209 1504
Fragments arranged by size:
1504 333 264 209
EagI C'GGCC_G
Cuts at: 0 264 2310
Size: 264 2046
EarI CTCTTCn'nnn_
Cuts at: 0 888 1858 2095 2310
Size: 888 970 237 215
Fragments arranged by size:
970 888 237 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 661 1438 1504 2310
Size: 661 777 66 806
Fragments arranged by size:
806 777 661 66
EcoICRI GAG'CTC
Cuts at: 0 675 1386 1605 2126 2310
Size: 675 711 219 521 184
Fragments arranged by size:
711 675 521 219 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1327 1333 1433 1594 2131 2310
Size: 457 870 6 100 161 537 179
Fragments arranged by size:
870 537 457 179 161 100 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1320 1587 1588 1839 2310
Size: 1320 267 1 251 471
Fragments arranged by size:
1320 471 267 251 1
FspI TGC'GCA
Cuts at: 0 1219 2310
Size: 1219 1091
HaeII r_GCGC'y
Cuts at: 0 47 164 838 1232 1510 2153 2204 2310
Size: 47 117 674 394 278 643 51 106
Fragments arranged by size:
674 643 394 278 117 106 51 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 565 597 2310
Size: 116 32 417 32 1713
Fragments arranged by size:
1713 417 116 32 32
HincII GTy'rAC
Cuts at: 0 978 1365 2310
Size: 978 387 945
Fragments arranged by size:
978 945 387
HindIII A'AGCT_T
Cuts at: 0 355 1783 2310
Size: 355 1428 527
Fragments arranged by size:
1428 527 355
HpaI GTT'AAC
Cuts at: 0 978 2310
Size: 978 1332
KasI G'GCGC_C
Cuts at: 0 1228 1506 2200 2310
Size: 1228 278 694 110
Fragments arranged by size:
1228 694 278 110
MscI TGG'CCA
Cuts at: 0 808 2310
Size: 808 1502
MslI CAynn'nnrTG
Cuts at: 0 725 871 940 1501 1799 2310
Size: 725 146 69 561 298 511
Fragments arranged by size:
725 561 511 298 146 69
MspA1I CmG'CkG
Cuts at: 0 1717 1743 2220 2277 2310
Size: 1717 26 477 57 33
Fragments arranged by size:
1717 477 57 33 26
NaeI GCC'GGC
Cuts at: 0 268 2310
Size: 268 2042
NarI GG'CG_CC
Cuts at: 0 1229 1507 2201 2310
Size: 1229 278 694 109
Fragments arranged by size:
1229 694 278 109
NcoI C'CATG_G
Cuts at: 0 866 1502 2310
Size: 866 636 808
Fragments arranged by size:
866 808 636
NgoMIV G'CCGG_C
Cuts at: 0 266 2310
Size: 266 2044
NspI r_CATG'y
Cuts at: 0 446 780 2310
Size: 446 334 1530
Fragments arranged by size:
1530 446 334
PasI CC'CwG_GG
Cuts at: 0 462 1594 2310
Size: 462 1132 716
Fragments arranged by size:
1132 716 462
PciI A'CATG_T
Cuts at: 0 442 2310
Size: 442 1868
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 642 2310
Size: 339 303 1668
Fragments arranged by size:
1668 339 303
PmlI CAC'GTG
Cuts at: 0 516 2310
Size: 516 1794
PpuMI rG'GwC_Cy
Cuts at: 0 1839 2310
Size: 1839 471
PspOMI G'GGCC_C
Cuts at: 0 1247 1587 2310
Size: 1247 340 723
Fragments arranged by size:
1247 723 340
PspXI vC'TCGA_Gb
Cuts at: 0 414 1126 2310
Size: 414 712 1184
Fragments arranged by size:
1184 712 414
PstI C_TGCA'G
Cuts at: 0 43 106 185 545 833 1298 1400 1421
Size: 43 63 79 360 288 465 102 21
Cuts at: 1421 1436 1622 2165 2310
Size: 15 186 543 145
Fragments arranged by size:
543 465 360 288 186 145 102 79
63 43 21 15
PvuII CAG'CTG
Cuts at: 0 1717 2220 2277 2310
Size: 1717 503 57 33
Fragments arranged by size:
1717 503 57 33
SacI G_AGCT'C
Cuts at: 0 677 1388 1607 2128 2310
Size: 677 711 219 521 182
Fragments arranged by size:
711 677 521 219 182
SanDI GG'GwC_CC
Cuts at: 0 1839 2310
Size: 1839 471
SfcI C'TryA_G
Cuts at: 0 39 102 181 541 829 1294 1396 1417
Size: 39 63 79 360 288 465 102 21
Cuts at: 1417 1432 1618 2161 2310
Size: 15 186 543 149
Fragments arranged by size:
543 465 360 288 186 149 102 79
63 39 21 15
SfoI GGC'GCC
Cuts at: 0 1230 1508 2202 2310
Size: 1230 278 694 108
Fragments arranged by size:
1230 694 278 108
SgrAI Cr'CCGG_yG
Cuts at: 0 1225 2310
Size: 1225 1085
SmlI C'TyrA_G
Cuts at: 0 414 1126 2310
Size: 414 712 1184
Fragments arranged by size:
1184 712 414
StuI AGG'CCT
Cuts at: 0 1543 2310
Size: 1543 767
StyI C'CwwG_G
Cuts at: 0 524 866 1502 2261 2310
Size: 524 342 636 759 49
Fragments arranged by size:
759 636 524 342 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 503 621 1515 1916 2089 2310
Size: 454 49 118 894 401 173 221
Fragments arranged by size:
894 454 401 221 173 118 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1134 1166 1457 1489 2310
Size: 1134 32 291 32 821
Fragments arranged by size:
1134 821 291 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 292 2310
Size: 292 2018
XbaI T'CTAG_A
Cuts at: 0 1872 2310
Size: 1872 438
XhoI C'TCGA_G
Cuts at: 0 414 1126 2310
Size: 414 712 1184
Fragments arranged by size:
1184 712 414
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I HincII HindIII HpaI KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmlI PpuMI PspOMI PspXI PstI PvuII
SacI SanDI SfcI SfoI SgrAI SmlI StuI StyI
TaqII TstI Tth111I XbaI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BciVI BfrBI BmtI BpuEI BsaBI BsiWI BsmI BsmBI
BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI
FspAI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PfoI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI XcmI XmaI XmnI ZraI