Restriction map for
pF1KB4619
(Linear) (Six-base) MAPSORT of: KIBB4619.seq Check: 2400 from: 1 to: 1152
KIBB4619.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 0 859 1152
Size: 859 293
AflIII A'CryG_T
Cuts at: 0 292 1152
Size: 292 860
AlwNI CAG_nnn'CTG
Cuts at: 0 334 1152
Size: 334 818
ApoI r'AATT_y
Cuts at: 0 410 733 1152
Size: 410 323 419
Fragments arranged by size:
419 410 323
AvrII C'CTAG_G
Cuts at: 0 904 1152
Size: 904 248
BamHI G'GATC_C
Cuts at: 0 309 828 1152
Size: 309 519 324
Fragments arranged by size:
519 324 309
BanII G_rGCy'C
Cuts at: 0 137 1152
Size: 137 1015
BciVI GTATCCnnnnn_n'
Cuts at: 0 193 1152
Size: 193 959
BmrI ACTGGGnnnn_n'
Cuts at: 0 829 1152
Size: 829 323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 866 908 1118 1152
Size: 644 222 42 210 34
Fragments arranged by size:
644 222 210 42 34
BsaBI GATnn'nnATC
Cuts at: 0 245 1152
Size: 245 907
BsaHI Gr'CG_yC
Cuts at: 0 275 1152
Size: 275 877
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 477 507 864 894 1016 1046
Size: 76 30 371 30 357 30 122 30
Cuts at: 1046 1152
Size: 106
Fragments arranged by size:
371 357 122 106 76 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 206 1152
Size: 121 85 946
Fragments arranged by size:
946 121 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1152
Size: 563 589
BsiEI CG_ry'CG
Cuts at: 0 15 1152
Size: 15 1137
BsiHKAI G_wGCw'C
Cuts at: 0 137 619 1152
Size: 137 482 533
Fragments arranged by size:
533 482 137
BsmI GAATG_Cn'
Cuts at: 0 769 1152
Size: 769 383
Bsp1286I G_dGCh'C
Cuts at: 0 137 619 1152
Size: 137 482 533
Fragments arranged by size:
533 482 137
BsrBI CCG'CTC
Cuts at: 0 452 1152
Size: 452 700
BsrDI GCAATG_nn'
Cuts at: 0 992 1152
Size: 992 160
BsrGI T'GTAC_A
Cuts at: 0 484 1152
Size: 484 668
BstAPI GCAn_nnn'nTGC
Cuts at: 0 334 1152
Size: 334 818
BstYI r'GATC_y
Cuts at: 0 309 591 828 1152
Size: 309 282 237 324
Fragments arranged by size:
324 309 282 237
Bsu36I CC'TnA_GG
Cuts at: 0 897 956 1152
Size: 897 59 196
Fragments arranged by size:
897 196 59
BtgI C'CryG_G
Cuts at: 0 68 1152
Size: 68 1084
DraI TTT'AAA
Cuts at: 0 317 1152
Size: 317 835
DrdI GACnn_nn'nnGTC
Cuts at: 0 802 1152
Size: 802 350
EaeI y'GGCC_r
Cuts at: 0 12 65 321 1152
Size: 12 53 256 831
Fragments arranged by size:
831 256 53 12
EagI C'GGCC_G
Cuts at: 0 12 1152
Size: 12 1140
EcoICRI GAG'CTC
Cuts at: 0 135 1152
Size: 135 1017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 866 908 1118 1152
Size: 644 222 42 210 34
Fragments arranged by size:
644 222 210 42 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 959 1152
Size: 959 193
EcoO109I rG'GnC_Cy
Cuts at: 0 901 1152
Size: 901 251
EcoRV GAT'ATC
Cuts at: 0 716 946 1057 1152
Size: 716 230 111 95
Fragments arranged by size:
716 230 111 95
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 985 1152
Size: 985 167
HaeII r_GCGC'y
Cuts at: 0 45 1152
Size: 45 1107
HincII GTy'rAC
Cuts at: 0 860 1152
Size: 860 292
MfeI C'AATT_G
Cuts at: 0 800 1152
Size: 800 352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 1152
Size: 1002 150
MscI TGG'CCA
Cuts at: 0 323 1152
Size: 323 829
MslI CAynn'nnrTG
Cuts at: 0 997 1152
Size: 997 155
NcoI C'CATG_G
Cuts at: 0 68 1152
Size: 68 1084
NspI r_CATG'y
Cuts at: 0 296 1152
Size: 296 856
PciI A'CATG_T
Cuts at: 0 292 1152
Size: 292 860
PfoI T'CCnGG_A
Cuts at: 0 939 1152
Size: 939 213
PpuMI rG'GwC_Cy
Cuts at: 0 901 1152
Size: 901 251
PsiI TTA'TAA
Cuts at: 0 185 1152
Size: 185 967
SacI G_AGCT'C
Cuts at: 0 137 1152
Size: 137 1015
SalI G'TCGA_C
Cuts at: 0 858 1152
Size: 858 294
ScaI AGT'ACT
Cuts at: 0 822 1152
Size: 822 330
StuI AGG'CCT
Cuts at: 0 909 1152
Size: 909 243
StyI C'CwwG_G
Cuts at: 0 68 904 1152
Size: 68 836 248
Fragments arranged by size:
836 248 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 1152
Size: 241 911
TatI w'GTAC_w
Cuts at: 0 83 484 820 1152
Size: 83 401 336 332
Fragments arranged by size:
401 336 332 83
XmnI GAAnn'nnTTC
Cuts at: 0 410 1152
Size: 410 742
Enzymes that do cut:
AccI AflIII AlwNI ApoI AvrII BamHI BanII BciVI
BmrI BpmI BsaBI BsaHI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI BstAPI BstYI
Bsu36I BtgI DraI DrdI EaeI EagI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII HincII MfeI MmeI
MscI MslI NcoI NspI PciI PfoI PpuMI PsiI
SacI SalI ScaI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BanI BbeI BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaWI BseYI BsiWI
BsmBI BspEI BspHI BspMI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraIII
EarI EciI EcoRI FseI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI