Restriction map for
pF1KB4618
(Linear) (Six-base) MAPSORT of: KIBB4618.seq Check: 9966 from: 1 to: 2238
KIBB4618.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 1906 2238
Size: 1906 332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1526 2111 2230 2238
Size: 1163 363 585 119 8
Fragments arranged by size:
1163 585 363 119 8
AfeI AGC'GCT
Cuts at: 0 979 2238
Size: 979 1259
AflIII A'CryG_T
Cuts at: 0 347 349 653 2238
Size: 347 2 304 1585
Fragments arranged by size:
1585 347 304 2
AgeI A'CCGG_T
Cuts at: 0 1766 2238
Size: 1766 472
AleI CACnn'nnGTG
Cuts at: 0 1507 2238
Size: 1507 731
AlwNI CAG_nnn'CTG
Cuts at: 0 1681 2238
Size: 1681 557
ApaI G_GGCC'C
Cuts at: 0 714 2238
Size: 714 1524
ApaLI G'TGCA_C
Cuts at: 0 2052 2238
Size: 2052 186
ApoI r'AATT_y
Cuts at: 0 1756 2035 2238
Size: 1756 279 203
Fragments arranged by size:
1756 279 203
AvaI C'yCGr_G
Cuts at: 0 464 1782 2238
Size: 464 1318 456
Fragments arranged by size:
1318 464 456
BanI G'GyrC_C
Cuts at: 0 6 1518 2238
Size: 6 1512 720
Fragments arranged by size:
1512 720 6
BanII G_rGCy'C
Cuts at: 0 714 1056 2238
Size: 714 342 1182
Fragments arranged by size:
1182 714 342
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 990 2124 2238
Size: 145 845 1134 114
Fragments arranged by size:
1134 845 145 114
BbvCI CC'TCA_GC
Cuts at: 0 797 2238
Size: 797 1441
BglI GCCn_nnn'nGGC
Cuts at: 0 718 2238
Size: 718 1520
Bme1580I G_kGCm'C
Cuts at: 0 714 1523 2056 2238
Size: 714 809 533 182
Fragments arranged by size:
809 714 533 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 1517 1877 1947 2238
Size: 1517 360 70 291
Fragments arranged by size:
1517 360 291 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 883 1556 2238
Size: 683 200 673 682
Fragments arranged by size:
683 682 673 200
Bpu10I CC'TnA_GC
Cuts at: 0 797 2238
Size: 797 1441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 2134 2238
Size: 1427 707 104
Fragments arranged by size:
1427 707 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 868 2238
Size: 868 1370
BsaAI yAC'GTr
Cuts at: 0 350 426 740 2238
Size: 350 76 314 1498
Fragments arranged by size:
1498 350 314 76
BsaBI GATnn'nnATC
Cuts at: 0 362 2238
Size: 362 1876
BsaHI Gr'CG_yC
Cuts at: 0 440 2238
Size: 440 1798
BsaWI w'CCGG_w
Cuts at: 0 1331 1766 2238
Size: 1331 435 472
Fragments arranged by size:
1331 472 435
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 905 935 1403 1433 2238
Size: 445 30 430 30 468 30 805
Fragments arranged by size:
805 468 445 430 30 30 30
BseYI C'CCAG_C
Cuts at: 0 608 1059 2238
Size: 608 451 1179
Fragments arranged by size:
1179 608 451
BsiEI CG_ry'CG
Cuts at: 0 1476 2238
Size: 1476 762
BsiHKAI G_wGCw'C
Cuts at: 0 1056 1865 2056 2238
Size: 1056 809 191 182
Fragments arranged by size:
1056 809 191 182
BsmI GAATG_Cn'
Cuts at: 0 347 770 2238
Size: 347 423 1468
Fragments arranged by size:
1468 423 347
BsmBI CGTCTCn'nnnn_
Cuts at: 0 447 2238
Size: 447 1791
Bsp1286I G_dGCh'C
Cuts at: 0 714 1056 1523 1865 2056 2238
Size: 714 342 467 342 191 182
Fragments arranged by size:
714 467 342 342 191 182
BsrBI CCG'CTC
Cuts at: 0 321 2238
Size: 321 1917
BsrDI GCAATG_nn'
Cuts at: 0 194 1112 1298 2238
Size: 194 918 186 940
Fragments arranged by size:
940 918 194 186
BsrFI r'CCGG_y
Cuts at: 0 282 749 1766 2238
Size: 282 467 1017 472
Fragments arranged by size:
1017 472 467 282
BsrGI T'GTAC_A
Cuts at: 0 1202 2183 2238
Size: 1202 981 55
Fragments arranged by size:
1202 981 55
BstEII G'GTnAC_C
Cuts at: 0 1777 2019 2238
Size: 1777 242 219
Fragments arranged by size:
1777 242 219
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 2238
Size: 1305 933
BstYI r'GATC_y
Cuts at: 0 327 804 1334 2238
Size: 327 477 530 904
Fragments arranged by size:
904 530 477 327
BstZ17I GTA'TAC
Cuts at: 0 1907 2238
Size: 1907 331
BtgI C'CryG_G
Cuts at: 0 31 829 1856 2238
Size: 31 798 1027 382
Fragments arranged by size:
1027 798 382 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 616 1094 2238
Size: 616 478 1144
Fragments arranged by size:
1144 616 478
BtsI GCAGTG_nn'
Cuts at: 0 574 2238
Size: 574 1664
ClaI AT'CG_AT
Cuts at: 0 554 2238
Size: 554 1684
DraIII CAC_nnn'GTG
Cuts at: 0 2094 2238
Size: 2094 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 439 2238
Size: 439 1799
EaeI y'GGCC_r
Cuts at: 0 28 488 1307 1688 1988 2238
Size: 28 460 819 381 300 250
Fragments arranged by size:
819 460 381 300 250 28
EarI CTCTTCn'nnn_
Cuts at: 0 21 59 175 1153 1420 1495 2206 2238
Size: 21 38 116 978 267 75 711 32
Fragments arranged by size:
978 711 267 116 75 38 32 21
EcoICRI GAG'CTC
Cuts at: 0 1054 2238
Size: 1054 1184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 883 1163 1526 1556 2111 2230 2238
Size: 683 200 280 363 30 555 119 8
Fragments arranged by size:
683 555 363 280 200 119 30 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 952 2238
Size: 952 1286
EcoO109I rG'GnC_Cy
Cuts at: 0 115 667 710 711 885 2238
Size: 115 552 43 1 174 1353
Fragments arranged by size:
1353 552 174 115 43 1
EcoRV GAT'ATC
Cuts at: 0 1704 1929 2238
Size: 1704 225 309
Fragments arranged by size:
1704 309 225
FspI TGC'GCA
Cuts at: 0 580 2238
Size: 580 1658
HaeII r_GCGC'y
Cuts at: 0 981 2238
Size: 981 1257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2130 2162 2238
Size: 2130 32 76
Fragments arranged by size:
2130 76 32
MscI TGG'CCA
Cuts at: 0 30 490 1309 1690 1990 2238
Size: 30 460 819 381 300 248
Fragments arranged by size:
819 460 381 300 248 30
MslI CAynn'nnrTG
Cuts at: 0 205 556 1507 2238
Size: 205 351 951 731
Fragments arranged by size:
951 731 351 205
MspA1I CmG'CkG
Cuts at: 0 796 1425 2238
Size: 796 629 813
Fragments arranged by size:
813 796 629
NcoI C'CATG_G
Cuts at: 0 1856 2238
Size: 1856 382
NdeI CA'TA_TG
Cuts at: 0 1344 1952 2238
Size: 1344 608 286
Fragments arranged by size:
1344 608 286
NspI r_CATG'y
Cuts at: 0 210 657 2238
Size: 210 447 1581
Fragments arranged by size:
1581 447 210
PciI A'CATG_T
Cuts at: 0 653 2238
Size: 653 1585
PmlI CAC'GTG
Cuts at: 0 350 2238
Size: 350 1888
PpuMI rG'GwC_Cy
Cuts at: 0 115 667 885 2238
Size: 115 552 218 1353
Fragments arranged by size:
1353 552 218 115
PsiI TTA'TAA
Cuts at: 0 680 2238
Size: 680 1558
PspOMI G'GGCC_C
Cuts at: 0 710 2238
Size: 710 1528
PstI C_TGCA'G
Cuts at: 0 1319 2238
Size: 1319 919
PvuII CAG'CTG
Cuts at: 0 796 2238
Size: 796 1442
SacI G_AGCT'C
Cuts at: 0 1056 2238
Size: 1056 1182
SapI GCTCTTCn'nnn_
Cuts at: 0 21 2206 2238
Size: 21 2185 32
Fragments arranged by size:
2185 32 21
ScaI AGT'ACT
Cuts at: 0 929 2238
Size: 929 1309
SexAI A'CCwGG_T
Cuts at: 0 822 992 2238
Size: 822 170 1246
Fragments arranged by size:
1246 822 170
SfcI C'TryA_G
Cuts at: 0 780 1018 1315 2238
Size: 780 238 297 923
Fragments arranged by size:
923 780 297 238
SmaI CCC'GGG
Cuts at: 0 466 1784 2238
Size: 466 1318 454
Fragments arranged by size:
1318 466 454
SmlI C'TyrA_G
Cuts at: 0 1442 2149 2238
Size: 1442 707 89
Fragments arranged by size:
1442 707 89
StyI C'CwwG_G
Cuts at: 0 1514 1856 2238
Size: 1514 342 382
Fragments arranged by size:
1514 382 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 84 1490 2238
Size: 84 1406 748
Fragments arranged by size:
1406 748 84
TatI w'GTAC_w
Cuts at: 0 875 927 1202 2183 2238
Size: 875 52 275 981 55
Fragments arranged by size:
981 875 275 55 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 905 937 1403 1435 2238
Size: 905 32 466 32 803
Fragments arranged by size:
905 803 466 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 824 2176 2238
Size: 824 1352 62
Fragments arranged by size:
1352 824 62
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 907 1980 2238
Size: 183 724 1073 258
Fragments arranged by size:
1073 724 258 183
XmaI C'CCGG_G
Cuts at: 0 464 1782 2238
Size: 464 1318 456
Fragments arranged by size:
1318 464 456
XmnI GAAnn'nnTTC
Cuts at: 0 1682 2215 2238
Size: 1682 533 23
Fragments arranged by size:
1682 533 23
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AleI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I MscI MslI MspA1I NcoI NdeI NspI PciI
PmlI PpuMI PsiI PspOMI PstI PvuII SacI SapI
ScaI SexAI SfcI SmaI SmlI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BseRI BsgI BsiWI BspEI BspHI BspMI BssHII BssSI
BstAPI BstBI Bsu36I CspCI DraI EagI EciI EcoRI
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI ZraI