Restriction map for
pF1KB4613
(Linear) (Six-base) MAPSORT of: KIBB4613.seq Check: 6329 from: 1 to: 795
KIBB4613.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 795
Size: 457 338
AflIII A'CryG_T
Cuts at: 0 368 425 795
Size: 368 57 370
Fragments arranged by size:
370 368 57
AlwNI CAG_nnn'CTG
Cuts at: 0 117 795
Size: 117 678
ApaLI G'TGCA_C
Cuts at: 0 55 795
Size: 55 740
AvaI C'yCGr_G
Cuts at: 0 480 795
Size: 480 315
BanI G'GyrC_C
Cuts at: 0 352 727 795
Size: 352 375 68
Fragments arranged by size:
375 352 68
BanII G_rGCy'C
Cuts at: 0 570 795
Size: 570 225
BbeI G_GCGC'C
Cuts at: 0 731 795
Size: 731 64
BglI GCCn_nnn'nGGC
Cuts at: 0 221 795
Size: 221 574
BlpI GC'TnA_GC
Cuts at: 0 163 412 795
Size: 163 249 383
Fragments arranged by size:
383 249 163
Bme1580I G_kGCm'C
Cuts at: 0 59 355 795
Size: 59 296 440
Fragments arranged by size:
440 296 59
BmgBI CAC'GTC
Cuts at: 0 336 795
Size: 336 459
BmrI ACTGGGnnnn_n'
Cuts at: 0 325 795
Size: 325 470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 489 636 795
Size: 489 147 159
Fragments arranged by size:
489 159 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 295 592 664 795
Size: 24 271 297 72 131
Fragments arranged by size:
297 271 131 72 24
BsaHI Gr'CG_yC
Cuts at: 0 728 784 795
Size: 728 56 11
Fragments arranged by size:
728 56 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 795
Size: 478 317
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 795
Size: 142 653
BsiEI CG_ry'CG
Cuts at: 0 8 795
Size: 8 787
BsiHKAI G_wGCw'C
Cuts at: 0 59 795
Size: 59 736
Bsp1286I G_dGCh'C
Cuts at: 0 59 355 570 795
Size: 59 296 215 225
Fragments arranged by size:
296 225 215 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 795
Size: 383 412
BsrBI CCG'CTC
Cuts at: 0 162 795
Size: 162 633
BssHII G'CGCG_C
Cuts at: 0 224 795
Size: 224 571
BssSI C'ACGA_G
Cuts at: 0 492 795
Size: 492 303
BtgI C'CryG_G
Cuts at: 0 771 795
Size: 771 24
DraIII CAC_nnn'GTG
Cuts at: 0 777 795
Size: 777 18
DrdI GACnn_nn'nnGTC
Cuts at: 0 12 795
Size: 12 783
EaeI y'GGCC_r
Cuts at: 0 213 795
Size: 213 582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 197 321 484 795
Size: 197 124 163 311
Fragments arranged by size:
311 197 163 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 295 457 592 664 795
Size: 24 271 162 135 72 131
Fragments arranged by size:
271 162 135 131 72 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 710 795
Size: 121 589 85
Fragments arranged by size:
589 121 85
EcoO109I rG'GnC_Cy
Cuts at: 0 402 795
Size: 402 393
HaeII r_GCGC'y
Cuts at: 0 47 140 731 795
Size: 47 93 591 64
Fragments arranged by size:
591 93 64 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 146 178 226 258 463 495 690 722
Size: 146 32 48 32 205 32 195 32
Cuts at: 722 795
Size: 73
Fragments arranged by size:
205 195 146 73 48 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 727 795
Size: 727 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 795
Size: 440 355
MscI TGG'CCA
Cuts at: 0 215 795
Size: 215 580
MslI CAynn'nnrTG
Cuts at: 0 550 795
Size: 550 245
MspA1I CmG'CkG
Cuts at: 0 303 411 642 795
Size: 303 108 231 153
Fragments arranged by size:
303 231 153 108
NarI GG'CG_CC
Cuts at: 0 728 795
Size: 728 67
NspI r_CATG'y
Cuts at: 0 81 429 795
Size: 81 348 366
Fragments arranged by size:
366 348 81
PciI A'CATG_T
Cuts at: 0 425 795
Size: 425 370
PfoI T'CCnGG_A
Cuts at: 0 708 795
Size: 708 87
PshAI GACnn'nnGTC
Cuts at: 0 787 795
Size: 787 8
PstI C_TGCA'G
Cuts at: 0 146 394 410 795
Size: 146 248 16 385
Fragments arranged by size:
385 248 146 16
PvuII CAG'CTG
Cuts at: 0 411 642 795
Size: 411 231 153
Fragments arranged by size:
411 231 153
SfcI C'TryA_G
Cuts at: 0 24 142 390 406 795
Size: 24 118 248 16 389
Fragments arranged by size:
389 248 118 24 16
SfoI GGC'GCC
Cuts at: 0 729 795
Size: 729 66
SphI G_CATG'C
Cuts at: 0 81 795
Size: 81 714
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 233 795
Size: 233 562
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI AvaI BanI BanII BbeI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaHI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BssHII
BssSI BtgI DraIII DrdI EaeI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NspI PciI PfoI PshAI PstI PvuII SfcI
SfoI SphI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmtI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI