Restriction map for
pF1KB4604
(Linear) (Six-base) MAPSORT of: KIBB4604.seq Check: 5727 from: 1 to: 909
KIBB4604.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
Acc65I G'GTAC_C
Cuts at: 0 734 909
Size: 734 175
AflIII A'CryG_T
Cuts at: 0 674 909
Size: 674 235
AlwNI CAG_nnn'CTG
Cuts at: 0 579 909
Size: 579 330
ApaLI G'TGCA_C
Cuts at: 0 387 909
Size: 387 522
ApoI r'AATT_y
Cuts at: 0 160 484 909
Size: 160 324 425
Fragments arranged by size:
425 324 160
AvaI C'yCGr_G
Cuts at: 0 650 909
Size: 650 259
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 104 137 909
Size: 104 33 772
Fragments arranged by size:
772 104 33
BanI G'GyrC_C
Cuts at: 0 400 734 909
Size: 400 334 175
Fragments arranged by size:
400 334 175
BbvCI CC'TCA_GC
Cuts at: 0 56 909
Size: 56 853
BglI GCCn_nnn'nGGC
Cuts at: 0 40 909
Size: 40 869
Bme1580I G_kGCm'C
Cuts at: 0 391 403 909
Size: 391 12 506
Fragments arranged by size:
506 391 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 637 909
Size: 415 222 272
Fragments arranged by size:
415 272 222
Bpu10I CC'TnA_GC
Cuts at: 0 56 909
Size: 56 853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 909
Size: 496 413
BsaI GGTCTCn'nnnn_
Cuts at: 0 620 805 909
Size: 620 185 104
Fragments arranged by size:
620 185 104
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 909
Size: 205 704
BseYI C'CCAG_C
Cuts at: 0 303 909
Size: 303 606
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 354 909
Size: 194 160 555
Fragments arranged by size:
555 194 160
BsiHKAI G_wGCw'C
Cuts at: 0 391 771 909
Size: 391 380 138
Fragments arranged by size:
391 380 138
Bsp1286I G_dGCh'C
Cuts at: 0 391 403 771 909
Size: 391 12 368 138
Fragments arranged by size:
391 368 138 12
BsrDI GCAATG_nn'
Cuts at: 0 345 789 909
Size: 345 444 120
Fragments arranged by size:
444 345 120
BsrFI r'CCGG_y
Cuts at: 0 859 909
Size: 859 50
BsrGI T'GTAC_A
Cuts at: 0 677 690 909
Size: 677 13 219
Fragments arranged by size:
677 219 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 245 579 909
Size: 245 334 330
Fragments arranged by size:
334 330 245
BstEII G'GTnAC_C
Cuts at: 0 525 909
Size: 525 384
BstYI r'GATC_y
Cuts at: 0 11 593 897 909
Size: 11 582 304 12
Fragments arranged by size:
582 304 12 11
BtgI C'CryG_G
Cuts at: 0 635 782 909
Size: 635 147 127
Fragments arranged by size:
635 147 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 825 909
Size: 825 84
BtsI GCAGTG_nn'
Cuts at: 0 225 760 909
Size: 225 535 149
Fragments arranged by size:
535 225 149
DraIII CAC_nnn'GTG
Cuts at: 0 386 636 766 909
Size: 386 250 130 143
Fragments arranged by size:
386 250 143 130
EaeI y'GGCC_r
Cuts at: 0 32 705 909
Size: 32 673 204
Fragments arranged by size:
673 204 32
EarI CTCTTCn'nnn_
Cuts at: 0 182 366 909
Size: 182 184 543
Fragments arranged by size:
543 184 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 217 909
Size: 217 692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 637 909
Size: 415 222 272
Fragments arranged by size:
415 272 222
EcoO109I rG'GnC_Cy
Cuts at: 0 5 41 288 909
Size: 5 36 247 621
Fragments arranged by size:
621 247 36 5
EcoRI G'AATT_C
Cuts at: 0 484 909
Size: 484 425
EcoRV GAT'ATC
Cuts at: 0 888 909
Size: 888 21
FspI TGC'GCA
Cuts at: 0 496 909
Size: 496 413
HaeII r_GCGC'y
Cuts at: 0 615 909
Size: 615 294
KpnI G_GTAC'C
Cuts at: 0 738 909
Size: 738 171
MslI CAynn'nnrTG
Cuts at: 0 271 277 602 781 909
Size: 271 6 325 179 128
Fragments arranged by size:
325 271 179 128 6
MspA1I CmG'CkG
Cuts at: 0 299 711 909
Size: 299 412 198
Fragments arranged by size:
412 299 198
NaeI GCC'GGC
Cuts at: 0 861 909
Size: 861 48
NcoI C'CATG_G
Cuts at: 0 782 909
Size: 782 127
NgoMIV G'CCGG_C
Cuts at: 0 859 909
Size: 859 50
NspI r_CATG'y
Cuts at: 0 501 678 909
Size: 501 177 231
Fragments arranged by size:
501 231 177
PciI A'CATG_T
Cuts at: 0 674 909
Size: 674 235
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 909
Size: 636 273
PpuMI rG'GwC_Cy
Cuts at: 0 288 909
Size: 288 621
PvuII CAG'CTG
Cuts at: 0 299 711 909
Size: 299 412 198
Fragments arranged by size:
412 299 198
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 40 909
Size: 40 869
SmaI CCC'GGG
Cuts at: 0 652 909
Size: 652 257
SmlI C'TyrA_G
Cuts at: 0 511 909
Size: 511 398
SphI G_CATG'C
Cuts at: 0 501 909
Size: 501 408
StyI C'CwwG_G
Cuts at: 0 44 782 909
Size: 44 738 127
Fragments arranged by size:
738 127 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 909
Size: 496 413
TatI w'GTAC_w
Cuts at: 0 677 690 836 909
Size: 677 13 146 73
Fragments arranged by size:
677 146 73 13
XmaI C'CCGG_G
Cuts at: 0 650 909
Size: 650 259
Enzymes that do cut:
Acc65I AflIII AlwNI ApaLI ApoI AvaI BaeI BanI
BbvCI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI BstAPI
BstEII BstYI BtgI BtgZI BtsI DraIII EaeI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII KpnI
MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI
PpuMI PvuII SfiI SmaI SmlI SphI StyI TaqII
TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BamHI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI FalI FseI FspAI Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI MscI NarI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI