Restriction map for
pF1KB4600
(Linear) (Six-base) MAPSORT of: KIBB4600.seq Check: 5107 from: 1 to: 918
KIBB4600.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 274 918
Size: 274 644
AclI AA'CG_TT
Cuts at: 0 11 918
Size: 11 907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 918
Size: 562 356
AflIII A'CryG_T
Cuts at: 0 423 918
Size: 423 495
AleI CACnn'nnGTG
Cuts at: 0 76 918
Size: 76 842
AvaI C'yCGr_G
Cuts at: 0 188 449 918
Size: 188 261 469
Fragments arranged by size:
469 261 188
BbvCI CC'TCA_GC
Cuts at: 0 782 869 918
Size: 782 87 49
Fragments arranged by size:
782 87 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 92 126 918
Size: 92 34 792
Fragments arranged by size:
792 92 34
BclI T'GATC_A
Cuts at: 0 845 918
Size: 845 73
Bme1580I G_kGCm'C
Cuts at: 0 464 918
Size: 464 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 918
Size: 859 59
Bpu10I CC'TnA_GC
Cuts at: 0 782 869 918
Size: 782 87 49
Fragments arranged by size:
782 87 49
BsaAI yAC'GTr
Cuts at: 0 424 918
Size: 424 494
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 348 422 425 428 718 799 918
Size: 348 74 3 3 290 81 119
Fragments arranged by size:
348 290 119 81 74 3 3
BseYI C'CCAG_C
Cuts at: 0 417 918
Size: 417 501
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 918
Size: 788 130
Bsp1286I G_dGCh'C
Cuts at: 0 464 918
Size: 464 454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 274 918
Size: 274 644
BsrGI T'GTAC_A
Cuts at: 0 407 918
Size: 407 511
BstBI TT'CG_AA
Cuts at: 0 112 918
Size: 112 806
BstYI r'GATC_y
Cuts at: 0 27 541 918
Size: 27 514 377
Fragments arranged by size:
514 377 27
EaeI y'GGCC_r
Cuts at: 0 2 228 918
Size: 2 226 690
Fragments arranged by size:
690 226 2
EarI CTCTTCn'nnn_
Cuts at: 0 695 918
Size: 695 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 859 918
Size: 562 297 59
Fragments arranged by size:
562 297 59
EcoO109I rG'GnC_Cy
Cuts at: 0 792 918
Size: 792 126
HaeII r_GCGC'y
Cuts at: 0 899 918
Size: 899 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 19 47 51 918
Size: 15 4 28 4 867
Fragments arranged by size:
867 28 15 4 4
MscI TGG'CCA
Cuts at: 0 4 230 918
Size: 4 226 688
Fragments arranged by size:
688 226 4
MslI CAynn'nnrTG
Cuts at: 0 76 918
Size: 76 842
MspA1I CmG'CkG
Cuts at: 0 304 537 837 918
Size: 304 233 300 81
Fragments arranged by size:
304 300 233 81
PfoI T'CCnGG_A
Cuts at: 0 629 918
Size: 629 289
PmlI CAC'GTG
Cuts at: 0 424 918
Size: 424 494
PpuMI rG'GwC_Cy
Cuts at: 0 792 918
Size: 792 126
PstI C_TGCA'G
Cuts at: 0 258 303 918
Size: 258 45 615
Fragments arranged by size:
615 258 45
PvuII CAG'CTG
Cuts at: 0 837 918
Size: 837 81
SfcI C'TryA_G
Cuts at: 0 197 254 299 455 918
Size: 197 57 45 156 463
Fragments arranged by size:
463 197 156 57 45
SmaI CCC'GGG
Cuts at: 0 190 918
Size: 190 728
SrfI GCCC'GGGC
Cuts at: 0 190 918
Size: 190 728
TatI w'GTAC_w
Cuts at: 0 407 918
Size: 407 511
XmaI C'CCGG_G
Cuts at: 0 188 918
Size: 188 730
XmnI GAAnn'nnTTC
Cuts at: 0 492 918
Size: 492 426
Enzymes that do cut:
AarI AclI AcuI AflIII AleI AvaI BbvCI BcgI
BclI Bme1580I BpmI Bpu10I BsaAI BseRI BseYI BsgI
Bsp1286I BspMI BsrGI BstBI BstYI EaeI EarI Eco57MI
EcoO109I HaeII Hin4I MscI MslI MspA1I PfoI PmlI
PpuMI PstI PvuII SfcI SmaI SrfI TatI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbsI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI ZraI