Restriction map for
pF1KB4585
(Linear) (Six-base) MAPSORT of: KIBB4585.seq Check: 6154 from: 1 to: 1038
KIBB4585.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 307 1038
Size: 307 731
AccI GT'mk_AC
Cuts at: 0 983 1038
Size: 983 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1038
Size: 808 230
AflIII A'CryG_T
Cuts at: 0 287 1038
Size: 287 751
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 275 1038
Size: 275 763
AvaI C'yCGr_G
Cuts at: 0 194 1038
Size: 194 844
BanI G'GyrC_C
Cuts at: 0 733 803 886 974 1038
Size: 733 70 83 88 64
Fragments arranged by size:
733 88 83 70 64
BanII G_rGCy'C
Cuts at: 0 101 115 601 896 1015 1038
Size: 101 14 486 295 119 23
Fragments arranged by size:
486 295 119 101 23 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 258 292 1038
Size: 258 34 746
Fragments arranged by size:
746 258 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 114 1038
Size: 114 924
BglI GCCn_nnn'nGGC
Cuts at: 0 580 1038
Size: 580 458
Bme1580I G_kGCm'C
Cuts at: 0 889 977 1038
Size: 889 88 61
Fragments arranged by size:
889 88 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 129 391 445 1038
Size: 115 14 262 54 593
Fragments arranged by size:
593 262 115 54 14
Bpu10I CC'TnA_GC
Cuts at: 0 248 518 1038
Size: 248 270 520
Fragments arranged by size:
520 270 248
BsaHI Gr'CG_yC
Cuts at: 0 514 1038
Size: 514 524
BseYI C'CCAG_C
Cuts at: 0 541 895 1038
Size: 541 354 143
Fragments arranged by size:
541 354 143
BsiHKAI G_wGCw'C
Cuts at: 0 115 1038
Size: 115 923
Bsp1286I G_dGCh'C
Cuts at: 0 101 115 601 889 896 977 1015 1038
Size: 101 14 486 288 7 81 38 23
Fragments arranged by size:
486 288 101 81 38 23 14 7
BspHI T'CATG_A
Cuts at: 0 482 1038
Size: 482 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 307 1038
Size: 307 731
BsrFI r'CCGG_y
Cuts at: 0 65 736 1038
Size: 65 671 302
Fragments arranged by size:
671 302 65
BstEII G'GTnAC_C
Cuts at: 0 294 1038
Size: 294 744
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 93 291 363 1038
Size: 81 12 198 72 675
Fragments arranged by size:
675 198 81 72 12
BstYI r'GATC_y
Cuts at: 0 498 1038
Size: 498 540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 58 1038
Size: 58 980
BtsI GCAGTG_nn'
Cuts at: 0 879 1038
Size: 879 159
DrdI GACnn_nn'nnGTC
Cuts at: 0 562 1038
Size: 562 476
EaeI y'GGCC_r
Cuts at: 0 83 572 854 1038
Size: 83 489 282 184
Fragments arranged by size:
489 282 184 83
EarI CTCTTCn'nnn_
Cuts at: 0 638 643 1038
Size: 638 5 395
Fragments arranged by size:
638 395 5
EcoICRI GAG'CTC
Cuts at: 0 113 1038
Size: 113 925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 129 391 445 808 1038
Size: 115 14 262 54 363 230
Fragments arranged by size:
363 262 230 115 54 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 303 1038
Size: 303 735
EcoO109I rG'GnC_Cy
Cuts at: 0 582 684 1038
Size: 582 102 354
Fragments arranged by size:
582 354 102
HaeII r_GCGC'y
Cuts at: 0 61 1038
Size: 61 977
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 1038
Size: 88 32 918
Fragments arranged by size:
918 88 32
MscI TGG'CCA
Cuts at: 0 85 574 856 1038
Size: 85 489 282 182
Fragments arranged by size:
489 282 182 85
MspA1I CmG'CkG
Cuts at: 0 81 259 525 680 825 883 1038
Size: 81 178 266 155 145 58 155
Fragments arranged by size:
266 178 155 155 145 81 58
NaeI GCC'GGC
Cuts at: 0 67 1038
Size: 67 971
NgoMIV G'CCGG_C
Cuts at: 0 65 1038
Size: 65 973
PasI CC'CwG_GG
Cuts at: 0 603 1038
Size: 603 435
PfoI T'CCnGG_A
Cuts at: 0 106 341 1038
Size: 106 235 697
Fragments arranged by size:
697 235 106
PpuMI rG'GwC_Cy
Cuts at: 0 684 1038
Size: 684 354
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1012 1038
Size: 1012 26
PvuII CAG'CTG
Cuts at: 0 259 525 1038
Size: 259 266 513
Fragments arranged by size:
513 266 259
SacI G_AGCT'C
Cuts at: 0 115 1038
Size: 115 923
SanDI GG'GwC_CC
Cuts at: 0 684 1038
Size: 684 354
ScaI AGT'ACT
Cuts at: 0 223 1038
Size: 223 815
SexAI A'CCwGG_T
Cuts at: 0 236 1038
Size: 236 802
SfcI C'TryA_G
Cuts at: 0 264 923 1038
Size: 264 659 115
Fragments arranged by size:
659 264 115
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 580 1038
Size: 580 458
StyI C'CwwG_G
Cuts at: 0 707 863 1038
Size: 707 156 175
Fragments arranged by size:
707 175 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 1038
Size: 595 443
TatI w'GTAC_w
Cuts at: 0 221 1038
Size: 221 817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 420 1038
Size: 420 618
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AvaI BanI BanII
BcgI BciVI BglI Bme1580I BpmI Bpu10I BsaHI BseYI
BsiHKAI Bsp1286I BspHI BspMI BsrFI BstEII BstXI BstYI
BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MscI MspA1I NaeI NgoMIV PasI
PfoI PpuMI PsrI PvuII SacI SanDI ScaI SexAI
SfcI SfiI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacII
SalI SapI SbfI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI