Restriction map for
pF1KB4582
(Linear) (Six-base) MAPSORT of: KIBB4582.seq Check: 7017 from: 1 to: 1884
KIBB4582.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 163 275 1884
Size: 163 112 1609
Fragments arranged by size:
1609 163 112
AccI GT'mk_AC
Cuts at: 0 1338 1884
Size: 1338 546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 813 1690 1884
Size: 676 137 877 194
Fragments arranged by size:
877 676 194 137
AleI CACnn'nnGTG
Cuts at: 0 43 1884
Size: 43 1841
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1683 1715 1884
Size: 1683 32 169
Fragments arranged by size:
1683 169 32
AlwNI CAG_nnn'CTG
Cuts at: 0 684 908 992 1884
Size: 684 224 84 892
Fragments arranged by size:
892 684 224 84
ApaLI G'TGCA_C
Cuts at: 0 334 1441 1884
Size: 334 1107 443
Fragments arranged by size:
1107 443 334
ApoI r'AATT_y
Cuts at: 0 117 370 1884
Size: 117 253 1514
Fragments arranged by size:
1514 253 117
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1607 1640 1884
Size: 1607 33 244
Fragments arranged by size:
1607 244 33
BanII G_rGCy'C
Cuts at: 0 975 1884
Size: 975 909
BciVI GTATCCnnnnn_n'
Cuts at: 0 1303 1884
Size: 1303 581
BclI T'GATC_A
Cuts at: 0 804 1884
Size: 804 1080
BfrBI ATG'CAT
Cuts at: 0 812 1884
Size: 812 1072
Bme1580I G_kGCm'C
Cuts at: 0 338 924 1445 1884
Size: 338 586 521 439
Fragments arranged by size:
586 521 439 338
BmrI ACTGGGnnnn_n'
Cuts at: 0 1323 1884
Size: 1323 561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 540 596 1148 1884
Size: 92 448 56 552 736
Fragments arranged by size:
736 552 448 92 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 1143 1884
Size: 1143 741
BsaWI w'CCGG_w
Cuts at: 0 296 1375 1884
Size: 296 1079 509
Fragments arranged by size:
1079 509 296
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 569 599 957 987 1884
Size: 569 30 358 30 897
Fragments arranged by size:
897 569 358 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 593 1884
Size: 593 1291
BseYI C'CCAG_C
Cuts at: 0 1243 1884
Size: 1243 641
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 697 1005 1478 1884
Size: 21 676 308 473 406
Fragments arranged by size:
676 473 406 308 21
BsiHKAI G_wGCw'C
Cuts at: 0 338 1445 1884
Size: 338 1107 439
Fragments arranged by size:
1107 439 338
BsmI GAATG_Cn'
Cuts at: 0 171 1552 1884
Size: 171 1381 332
Fragments arranged by size:
1381 332 171
Bsp1286I G_dGCh'C
Cuts at: 0 338 924 975 1445 1884
Size: 338 586 51 470 439
Fragments arranged by size:
586 470 439 338 51
BspHI T'CATG_A
Cuts at: 0 1416 1605 1884
Size: 1416 189 279
Fragments arranged by size:
1416 279 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 163 275 1000 1151 1884
Size: 163 112 725 151 733
Fragments arranged by size:
733 725 163 151 112
BsrBI CCG'CTC
Cuts at: 0 437 1884
Size: 437 1447
BsrDI GCAATG_nn'
Cuts at: 0 246 798 1254 1884
Size: 246 552 456 630
Fragments arranged by size:
630 552 456 246
BsrFI r'CCGG_y
Cuts at: 0 414 938 1884
Size: 414 524 946
Fragments arranged by size:
946 524 414
BssHII G'CGCG_C
Cuts at: 0 11 1884
Size: 11 1873
BstAPI GCAn_nnn'nTGC
Cuts at: 0 908 992 1884
Size: 908 84 892
Fragments arranged by size:
908 892 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 448 1709 1884
Size: 429 19 1261 175
Fragments arranged by size:
1261 429 175 19
BstYI r'GATC_y
Cuts at: 0 204 347 1884
Size: 204 143 1537
Fragments arranged by size:
1537 204 143
BtgI C'CryG_G
Cuts at: 0 278 422 867 943 1884
Size: 278 144 445 76 941
Fragments arranged by size:
941 445 278 144 76
BtsI GCAGTG_nn'
Cuts at: 0 906 1884
Size: 906 978
DraI TTT'AAA
Cuts at: 0 1868 1884
Size: 1868 16
DraIII CAC_nnn'GTG
Cuts at: 0 314 1884
Size: 314 1570
DrdI GACnn_nn'nnGTC
Cuts at: 0 698 1884
Size: 698 1186
EaeI y'GGCC_r
Cuts at: 0 47 795 940 1884
Size: 47 748 145 944
Fragments arranged by size:
944 748 145 47
EarI CTCTTCn'nnn_
Cuts at: 0 722 1884
Size: 722 1162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 540 596 676 813 1148 1690 1884
Size: 92 448 56 80 137 335 542 194
Fragments arranged by size:
542 448 335 194 137 92 80 56
EcoO109I rG'GnC_Cy
Cuts at: 0 843 1884
Size: 843 1041
EcoRI G'AATT_C
Cuts at: 0 370 1884
Size: 370 1514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1146 1884
Size: 1146 738
FspI TGC'GCA
Cuts at: 0 1481 1884
Size: 1481 403
HaeII r_GCGC'y
Cuts at: 0 536 1884
Size: 536 1348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 332 364 1193 1225 1370 1402 1884
Size: 332 32 829 32 145 32 482
Fragments arranged by size:
829 482 332 145 32 32 32
HincII GTy'rAC
Cuts at: 0 1339 1884
Size: 1339 545
HindIII A'AGCT_T
Cuts at: 0 596 1815 1884
Size: 596 1219 69
Fragments arranged by size:
1219 596 69
MscI TGG'CCA
Cuts at: 0 797 1884
Size: 797 1087
MslI CAynn'nnrTG
Cuts at: 0 43 1884
Size: 43 1841
MspA1I CmG'CkG
Cuts at: 0 86 654 681 782 955 1207 1884
Size: 86 568 27 101 173 252 677
Fragments arranged by size:
677 568 252 173 101 86 27
NaeI GCC'GGC
Cuts at: 0 416 1884
Size: 416 1468
NcoI C'CATG_G
Cuts at: 0 278 422 867 943 1884
Size: 278 144 445 76 941
Fragments arranged by size:
941 445 278 144 76
NdeI CA'TA_TG
Cuts at: 0 1575 1884
Size: 1575 309
NgoMIV G'CCGG_C
Cuts at: 0 414 1884
Size: 414 1470
NsiI A_TGCA'T
Cuts at: 0 814 1884
Size: 814 1070
NspI r_CATG'y
Cuts at: 0 1012 1884
Size: 1012 872
PflMI CCAn_nnn'nTGG
Cuts at: 0 574 1884
Size: 574 1310
PshAI GACnn'nnGTC
Cuts at: 0 1433 1884
Size: 1433 451
PsiI TTA'TAA
Cuts at: 0 470 1884
Size: 470 1414
PvuII CAG'CTG
Cuts at: 0 654 681 782 955 1207 1884
Size: 654 27 101 173 252 677
Fragments arranged by size:
677 654 252 173 101 27
SalI G'TCGA_C
Cuts at: 0 1337 1884
Size: 1337 547
SapI GCTCTTCn'nnn_
Cuts at: 0 722 1884
Size: 722 1162
ScaI AGT'ACT
Cuts at: 0 475 1884
Size: 475 1409
SfcI C'TryA_G
Cuts at: 0 189 1884
Size: 189 1695
SphI G_CATG'C
Cuts at: 0 1012 1884
Size: 1012 872
SspI AAT'ATT
Cuts at: 0 1570 1884
Size: 1570 314
StyI C'CwwG_G
Cuts at: 0 278 422 867 943 1547 1884
Size: 278 144 445 76 604 337
Fragments arranged by size:
604 445 337 278 144 76
TatI w'GTAC_w
Cuts at: 0 179 473 541 1884
Size: 179 294 68 1343
Fragments arranged by size:
1343 294 179 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1047 1079 1884
Size: 1047 32 805
Fragments arranged by size:
1047 805 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 865 1466 1884
Size: 865 601 418
Fragments arranged by size:
865 601 418
XmnI GAAnn'nnTTC
Cuts at: 0 1675 1884
Size: 1675 209
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaLI ApoI
BaeI BanII BciVI BclI BfrBI Bme1580I BmrI BpmI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstXI BstYI BtgI BtsI DraI DraIII DrdI EaeI
EarI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NsiI NspI PflMI PshAI PsiI PvuII SalI
SapI ScaI SfcI SphI SspI StyI TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI AscI AseI AsiSI AvaI AvrII BamHI
BanI BbeI BbsI BbvCI BcgI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspEI BsrGI BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI EagI EciI EcoICRI
EcoNI EcoRV FseI FspAI HpaI KasI KpnI MfeI
MluI MmeI NarI NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI ZraI