Restriction map for
pF1KB4576
(Linear) (Six-base) MAPSORT of: KIBB4576.seq Check: 3687 from: 1 to: 567
KIBB4576.orf
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 567
Size: 85 482
AlwNI CAG_nnn'CTG
Cuts at: 0 191 567
Size: 191 376
BamHI G'GATC_C
Cuts at: 0 479 567
Size: 479 88
BanI G'GyrC_C
Cuts at: 0 322 567
Size: 322 245
BanII G_rGCy'C
Cuts at: 0 185 285 567
Size: 185 100 282
Fragments arranged by size:
282 185 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 73 558 567
Size: 73 485 9
Fragments arranged by size:
485 73 9
BbvCI CC'TCA_GC
Cuts at: 0 331 567
Size: 331 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 63 97 567
Size: 63 34 470
Fragments arranged by size:
470 63 34
BclI T'GATC_A
Cuts at: 0 344 567
Size: 344 223
BglI GCCn_nnn'nGGC
Cuts at: 0 180 235 317 567
Size: 180 55 82 250
Fragments arranged by size:
250 180 82 55
Bme1580I G_kGCm'C
Cuts at: 0 327 567
Size: 327 240
BmtI G_CTAG'C
Cuts at: 0 454 567
Size: 454 113
Bpu10I CC'TnA_GC
Cuts at: 0 184 331 567
Size: 184 147 236
Fragments arranged by size:
236 184 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 567
Size: 272 30 265
Fragments arranged by size:
272 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 567
Size: 319 248
BsiEI CG_ry'CG
Cuts at: 0 549 567
Size: 549 18
BsiHKAI G_wGCw'C
Cuts at: 0 215 567
Size: 215 352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 207 451 567
Size: 207 244 116
Fragments arranged by size:
244 207 116
Bsp1286I G_dGCh'C
Cuts at: 0 185 215 285 327 567
Size: 185 30 70 42 240
Fragments arranged by size:
240 185 70 42 30
BsrDI GCAATG_nn'
Cuts at: 0 153 224 567
Size: 153 71 343
Fragments arranged by size:
343 153 71
BssSI C'ACGA_G
Cuts at: 0 52 567
Size: 52 515
BstEII G'GTnAC_C
Cuts at: 0 288 567
Size: 288 279
BstYI r'GATC_y
Cuts at: 0 77 479 567
Size: 77 402 88
Fragments arranged by size:
402 88 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 103 567
Size: 103 464
BtsI GCAGTG_nn'
Cuts at: 0 440 567
Size: 440 127
EaeI y'GGCC_r
Cuts at: 0 5 546 567
Size: 5 541 21
Fragments arranged by size:
541 21 5
EagI C'GGCC_G
Cuts at: 0 546 567
Size: 546 21
EarI CTCTTCn'nnn_
Cuts at: 0 220 567
Size: 220 347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 298 567
Size: 298 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 567
Size: 85 482
HaeII r_GCGC'y
Cuts at: 0 241 567
Size: 241 326
HindIII A'AGCT_T
Cuts at: 0 407 567
Size: 407 160
NheI G'CTAG_C
Cuts at: 0 450 567
Size: 450 117
PasI CC'CwG_GG
Cuts at: 0 474 567
Size: 474 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 567
Size: 432 135
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 346 378 567
Size: 346 32 189
Fragments arranged by size:
346 189 32
SapI GCTCTTCn'nnn_
Cuts at: 0 220 567
Size: 220 347
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 272 304 567
Size: 272 32 263
Fragments arranged by size:
272 263 32
Enzymes that do cut:
AcuI AlwNI BamHI BanI BanII BbsI BbvCI BcgI
BclI BglI Bme1580I BmtI Bpu10I BsaXI BseRI BsiEI
BsiHKAI BsmBI Bsp1286I BsrDI BssSI BstEII BstYI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI HaeII HindIII
NheI PasI PflMI PsrI SapI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BciVI
BfrBI BglII BlpI BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI