Restriction map for
pF1KB4543
(Linear) (Six-base) MAPSORT of: KIBB4543.seq Check: 7728 from: 1 to: 3357
KIBB4543.orf
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 333 2400 3357
Size: 333 2067 957
Fragments arranged by size:
2067 957 333
AatII G_ACGT'C
Cuts at: 0 3258 3357
Size: 3258 99
AccI GT'mk_AC
Cuts at: 0 1670 1877 2176 3357
Size: 1670 207 299 1181
Fragments arranged by size:
1670 1181 299 207
AclI AA'CG_TT
Cuts at: 0 821 1664 3357
Size: 821 843 1693
Fragments arranged by size:
1693 843 821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 369 871 1178 1789 1795 3229 3357
Size: 205 164 502 307 611 6 1434 128
Fragments arranged by size:
1434 611 502 307 205 164 128 6
AflIII A'CryG_T
Cuts at: 0 674 1250 1779 3357
Size: 674 576 529 1578
Fragments arranged by size:
1578 674 576 529
AhdI GACnn_n'nnGTC
Cuts at: 0 1254 3357
Size: 1254 2103
AleI CACnn'nnGTG
Cuts at: 0 511 1109 1150 1168 3357
Size: 511 598 41 18 2189
Fragments arranged by size:
2189 598 511 41 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 969 2170 3357
Size: 969 1201 1187
Fragments arranged by size:
1201 1187 969
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 3357
Size: 127 32 3198
Fragments arranged by size:
3198 127 32
AlwNI CAG_nnn'CTG
Cuts at: 0 969 3327 3343 3357
Size: 969 2358 16 14
Fragments arranged by size:
2358 969 16 14
ApaI G_GGCC'C
Cuts at: 0 257 3357
Size: 257 3100
ApaLI G'TGCA_C
Cuts at: 0 289 954 3357
Size: 289 665 2403
Fragments arranged by size:
2403 665 289
AscI GG'CGCG_CC
Cuts at: 0 245 3357
Size: 245 3112
AvaI C'yCGr_G
Cuts at: 0 52 437 1048 1680 1973 2805 3357
Size: 52 385 611 632 293 832 552
Fragments arranged by size:
832 632 611 552 385 293 52
BanI G'GyrC_C
Cuts at: 0 9 58 345 643 1994 2209 2332 3089
Size: 9 49 287 298 1351 215 123 757
Cuts at: 3089 3357
Size: 268
Fragments arranged by size:
1351 757 298 287 268 215 123 49
9
BanII G_rGCy'C
Cuts at: 0 53 98 257 280 566 992 2101 2276
Size: 53 45 159 23 286 426 1109 175
Cuts at: 2276 2306 2720 3302 3357
Size: 30 414 582 55
Fragments arranged by size:
1109 582 426 414 286 175 159 55
53 45 30 23
BbeI G_GCGC'C
Cuts at: 0 13 62 3357
Size: 13 49 3295
Fragments arranged by size:
3295 49 13
BbvCI CC'TCA_GC
Cuts at: 0 2425 3357
Size: 2425 932
BciVI GTATCCnnnnn_n'
Cuts at: 0 2305 3357
Size: 2305 1052
BglI GCCn_nnn'nGGC
Cuts at: 0 67 2207 2452 3248 3357
Size: 67 2140 245 796 109
Fragments arranged by size:
2140 796 245 109 67
BlpI GC'TnA_GC
Cuts at: 0 2305 3357
Size: 2305 1052
Bme1580I G_kGCm'C
Cuts at: 0 257 293 350 958 1687 2212 3094 3271
Size: 257 36 57 608 729 525 882 177
Cuts at: 3271 3357
Size: 86
Fragments arranged by size:
882 729 608 525 257 177 86 57
36
BmgBI CAC'GTC
Cuts at: 0 625 1251 2282 2532 3357
Size: 625 626 1031 250 825
Fragments arranged by size:
1031 825 626 625 250
BmrI ACTGGGnnnn_n'
Cuts at: 0 945 2333 2976 3357
Size: 945 1388 643 381
Fragments arranged by size:
1388 945 643 381
BmtI G_CTAG'C
Cuts at: 0 2224 2797 3357
Size: 2224 573 560
Fragments arranged by size:
2224 573 560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 187 651 787 1759 2115 2320 2978
Size: 154 33 464 136 972 356 205 658
Cuts at: 2978 3357
Size: 379
Fragments arranged by size:
972 658 464 379 356 205 154 136
33
Bpu10I CC'TnA_GC
Cuts at: 0 2425 2842 3357
Size: 2425 417 515
Fragments arranged by size:
2425 515 417
BsaBI GATnn'nnATC
Cuts at: 0 883 3357
Size: 883 2474
BsaHI Gr'CG_yC
Cuts at: 0 10 59 2603 3255 3281 3357
Size: 10 49 2544 652 26 76
Fragments arranged by size:
2544 652 76 49 26 10
BsaWI w'CCGG_w
Cuts at: 0 332 3357
Size: 332 3025
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 1783 1813 3357
Size: 127 30 1626 30 1544
Fragments arranged by size:
1626 1544 127 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 787 970 2473 2476 2823 3357
Size: 127 660 183 1503 3 347 534
Fragments arranged by size:
1503 660 534 347 183 127 3
BseYI C'CCAG_C
Cuts at: 0 240 350 423 457 1082 2537 3295 3343
Size: 240 110 73 34 625 1455 758 48
Cuts at: 3343 3357
Size: 14
Fragments arranged by size:
1455 758 625 240 110 73 48 34
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1546 1948 3357
Size: 1180 366 402 1409
Fragments arranged by size:
1409 1180 402 366
BsiEI CG_ry'CG
Cuts at: 0 74 473 3357
Size: 74 399 2884
Fragments arranged by size:
2884 399 74
BsiHKAI G_wGCw'C
Cuts at: 0 293 958 992 2101 2306 2720 2773 2848
Size: 293 665 34 1109 205 414 53 75
Cuts at: 2848 3357
Size: 509
Fragments arranged by size:
1109 665 509 414 293 205 75 53
34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 631 2343 3288 3357
Size: 631 1712 945 69
Fragments arranged by size:
1712 945 631 69
Bsp1286I G_dGCh'C
Cuts at: 0 53 98 257 280 293 350 566 958
Size: 53 45 159 23 13 57 216 392
Cuts at: 958 992 1687 2101 2212 2276 2306 2720 2773
Size: 34 695 414 111 64 30 414 53
Cuts at: 2773 2848 3094 3271 3302 3357
Size: 75 246 177 31 55
Fragments arranged by size:
695 414 414 392 246 216 177 159
111 75 64 57 55 53 53 45
34 31 30 23 13
BspHI T'CATG_A
Cuts at: 0 800 1580 3357
Size: 800 780 1777
Fragments arranged by size:
1777 800 780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 351 1526 1594 1970 2400 3335 3357
Size: 333 18 1175 68 376 430 935 22
Fragments arranged by size:
1175 935 430 376 333 68 22 18
BsrBI CCG'CTC
Cuts at: 0 66 792 1646 3357
Size: 66 726 854 1711
Fragments arranged by size:
1711 854 726 66
BsrFI r'CCGG_y
Cuts at: 0 86 1482 2523 3074 3357
Size: 86 1396 1041 551 283
Fragments arranged by size:
1396 1041 551 283 86
BssHII G'CGCG_C
Cuts at: 0 218 245 3357
Size: 218 27 3112
Fragments arranged by size:
3112 218 27
BssSI C'ACGA_G
Cuts at: 0 316 565 3357
Size: 316 249 2792
Fragments arranged by size:
2792 316 249
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1533 3357
Size: 1533 1824
BstEII G'GTnAC_C
Cuts at: 0 321 504 3357
Size: 321 183 2853
Fragments arranged by size:
2853 321 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 629 1170 1284 2412 3024 3357
Size: 629 541 114 1128 612 333
Fragments arranged by size:
1128 629 612 541 333 114
BstYI r'GATC_y
Cuts at: 0 1173 1562 3357
Size: 1173 389 1795
Fragments arranged by size:
1795 1173 389
BtgI C'CryG_G
Cuts at: 0 147 827 1016 3357
Size: 147 680 189 2341
Fragments arranged by size:
2341 680 189 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1423 2685 3357
Size: 1423 1262 672
Fragments arranged by size:
1423 1262 672
BtsI GCAGTG_nn'
Cuts at: 0 291 1104 1536 3357
Size: 291 813 432 1821
Fragments arranged by size:
1821 813 432 291
DraIII CAC_nnn'GTG
Cuts at: 0 288 1900 3105 3357
Size: 288 1612 1205 252
Fragments arranged by size:
1612 1205 288 252
EaeI y'GGCC_r
Cuts at: 0 71 176 194 230 269 366 548 1274
Size: 71 105 18 36 39 97 182 726
Cuts at: 1274 1484 2366 2780 3014 3357
Size: 210 882 414 234 343
Fragments arranged by size:
882 726 414 343 234 210 182 105
97 71 39 36 18
EagI C'GGCC_G
Cuts at: 0 71 3357
Size: 71 3286
EarI CTCTTCn'nnn_
Cuts at: 0 307 388 664 997 2383 2515 3357
Size: 307 81 276 333 1386 132 842
Fragments arranged by size:
1386 842 333 307 276 132 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 47 435 2869 3357
Size: 35 12 388 2434 488
Fragments arranged by size:
2434 488 388 35 12
EcoICRI GAG'CTC
Cuts at: 0 990 2099 2304 2718 3357
Size: 990 1109 205 414 639
Fragments arranged by size:
1109 990 639 414 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 187 205 369 651 787 871 1178
Size: 154 33 18 164 282 136 84 307
Cuts at: 1178 1759 1789 1795 2115 2320 2978 3229 3357
Size: 581 30 6 320 205 658 251 128
Fragments arranged by size:
658 581 320 307 282 251 205 164
154 136 128 84 33 30 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 3357
Size: 271 3086
EcoO109I rG'GnC_Cy
Cuts at: 0 253 1209 2978 3197 3357
Size: 253 956 1769 219 160
Fragments arranged by size:
1769 956 253 219 160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 861 3357
Size: 861 2496
FspI TGC'GCA
Cuts at: 0 538 1234 3357
Size: 538 696 2123
Fragments arranged by size:
2123 696 538
HaeII r_GCGC'y
Cuts at: 0 13 62 142 193 229 313 379 621
Size: 13 49 80 51 36 84 66 242
Cuts at: 621 1835 2800 2972 3357
Size: 1214 965 172 385
Fragments arranged by size:
1214 965 385 242 172 84 80 66
51 49 36 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 2918 2950 2955 2987 3104 3136
Size: 1240 32 1646 32 5 32 117 32
Cuts at: 3136 3357
Size: 221
Fragments arranged by size:
1646 1240 221 117 32 32 32 32
5
HincII GTy'rAC
Cuts at: 0 1695 3009 3149 3357
Size: 1695 1314 140 208
Fragments arranged by size:
1695 1314 208 140
KasI G'GCGC_C
Cuts at: 0 9 58 3357
Size: 9 49 3299
Fragments arranged by size:
3299 49 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2579 3001 3357
Size: 2579 422 356
Fragments arranged by size:
2579 422 356
MscI TGG'CCA
Cuts at: 0 271 550 1276 2368 3016 3357
Size: 271 279 726 1092 648 341
Fragments arranged by size:
1092 726 648 341 279 271
MslI CAynn'nnrTG
Cuts at: 0 511 832 899 951 1109 1150 1168 1186
Size: 511 321 67 52 158 41 18 18
Cuts at: 1186 2668 2776 2869 3357
Size: 1482 108 93 488
Fragments arranged by size:
1482 511 488 321 158 108 93 67
52 41 18 18
MspA1I CmG'CkG
Cuts at: 0 149 354 372 457 1294 2309 2785 3343
Size: 149 205 18 85 837 1015 476 558
Cuts at: 3343 3357
Size: 14
Fragments arranged by size:
1015 837 558 476 205 149 85 18
14
NaeI GCC'GGC
Cuts at: 0 88 2525 3357
Size: 88 2437 832
Fragments arranged by size:
2437 832 88
NarI GG'CG_CC
Cuts at: 0 10 59 3357
Size: 10 49 3298
Fragments arranged by size:
3298 49 10
NcoI C'CATG_G
Cuts at: 0 827 3357
Size: 827 2530
NgoMIV G'CCGG_C
Cuts at: 0 86 2523 3357
Size: 86 2437 834
Fragments arranged by size:
2437 834 86
NheI G'CTAG_C
Cuts at: 0 2220 2793 3357
Size: 2220 573 564
Fragments arranged by size:
2220 573 564
NspI r_CATG'y
Cuts at: 0 678 1185 1783 2404 3357
Size: 678 507 598 621 953
Fragments arranged by size:
953 678 621 598 507
PasI CC'CwG_GG
Cuts at: 0 3201 3357
Size: 3201 156
PciI A'CATG_T
Cuts at: 0 674 1779 3357
Size: 674 1105 1578
Fragments arranged by size:
1578 1105 674
PflMI CCAn_nnn'nTGG
Cuts at: 0 969 3357
Size: 969 2388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 3357
Size: 127 32 3198
Fragments arranged by size:
3198 127 32
PpuMI rG'GwC_Cy
Cuts at: 0 1209 3197 3357
Size: 1209 1988 160
Fragments arranged by size:
1988 1209 160
PspOMI G'GGCC_C
Cuts at: 0 253 3357
Size: 253 3104
PstI C_TGCA'G
Cuts at: 0 344 359 734 3339 3357
Size: 344 15 375 2605 18
Fragments arranged by size:
2605 375 344 18 15
PvuII CAG'CTG
Cuts at: 0 354 372 3343 3357
Size: 354 18 2971 14
Fragments arranged by size:
2971 354 18 14
SacI G_AGCT'C
Cuts at: 0 992 2101 2306 2720 3357
Size: 992 1109 205 414 637
Fragments arranged by size:
1109 992 637 414 205
SacII CC_GC'GG
Cuts at: 0 150 3357
Size: 150 3207
SapI GCTCTTCn'nnn_
Cuts at: 0 388 664 997 2383 3357
Size: 388 276 333 1386 974
Fragments arranged by size:
1386 974 388 333 276
SbfI CC_TGCA'GG
Cuts at: 0 344 734 3357
Size: 344 390 2623
Fragments arranged by size:
2623 390 344
SfcI C'TryA_G
Cuts at: 0 340 355 730 3335 3357
Size: 340 15 375 2605 22
Fragments arranged by size:
2605 375 340 22 15
SfoI GGC'GCC
Cuts at: 0 11 60 3357
Size: 11 49 3297
Fragments arranged by size:
3297 49 11
SgrAI Cr'CCGG_yG
Cuts at: 0 86 3357
Size: 86 3271
SmaI CCC'GGG
Cuts at: 0 54 439 1682 1975 3357
Size: 54 385 1243 293 1382
Fragments arranged by size:
1382 1243 385 293 54
SphI G_CATG'C
Cuts at: 0 2404 3357
Size: 2404 953
SspI AAT'ATT
Cuts at: 0 1889 3357
Size: 1889 1468
StuI AGG'CCT
Cuts at: 0 1460 3357
Size: 1460 1897
StyI C'CwwG_G
Cuts at: 0 272 602 827 1271 2061 2086 2813 3357
Size: 272 330 225 444 790 25 727 544
Fragments arranged by size:
790 727 544 444 330 272 225 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 770 1096 3357
Size: 770 326 2261
Fragments arranged by size:
2261 770 326
TatI w'GTAC_w
Cuts at: 0 1772 3357
Size: 1772 1585
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 627 659 2313 2345 3357
Size: 627 32 1654 32 1012
Fragments arranged by size:
1654 1012 627 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2917 3357
Size: 2917 440
XbaI T'CTAG_A
Cuts at: 0 3159 3357
Size: 3159 198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1149 2115 3357
Size: 1149 966 1242
Fragments arranged by size:
1242 1149 966
XmaI C'CCGG_G
Cuts at: 0 52 437 1680 1973 3357
Size: 52 385 1243 293 1384
Fragments arranged by size:
1384 1243 385 293 52
XmnI GAAnn'nnTTC
Cuts at: 0 724 1390 1549 3357
Size: 724 666 159 1808
Fragments arranged by size:
1808 724 666 159
ZraI GAC'GTC
Cuts at: 0 3256 3357
Size: 3256 101
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AvaI BanI
BanII BbeI BbvCI BciVI BglI BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI
SbfI SfcI SfoI SgrAI SmaI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI ApoI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BclI BfrBI BglII BplI
BpuEI BsaI BsaAI BsiWI BsmI BspEI BsrDI BsrGI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI
EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NotI NruI NsiI PacI PfoI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SrfI
SwaI XhoI