Restriction map for
pF1KB4540
(Linear) (Six-base) MAPSORT of: KIBB4540.seq Check: 3953 from: 1 to: 1044
KIBB4540.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 0 152 1044
Size: 152 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1044
Size: 382 662
AflII C'TTAA_G
Cuts at: 0 10 1044
Size: 10 1034
AflIII A'CryG_T
Cuts at: 0 883 1044
Size: 883 161
AlwNI CAG_nnn'CTG
Cuts at: 0 287 1044
Size: 287 757
AseI AT'TA_AT
Cuts at: 0 691 1044
Size: 691 353
BanII G_rGCy'C
Cuts at: 0 59 1044
Size: 59 985
BclI T'GATC_A
Cuts at: 0 234 1044
Size: 234 810
BfrBI ATG'CAT
Cuts at: 0 317 1044
Size: 317 727
BlpI GC'TnA_GC
Cuts at: 0 242 1044
Size: 242 802
Bme1580I G_kGCm'C
Cuts at: 0 433 1044
Size: 433 611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 837 1044
Size: 837 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 594 1044
Size: 493 101 450
Fragments arranged by size:
493 450 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 83 1044
Size: 43 40 961
Fragments arranged by size:
961 43 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 666 1044
Size: 13 653 378
Fragments arranged by size:
653 378 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 146 496 1044
Size: 146 350 548
Fragments arranged by size:
548 350 146
BseYI C'CCAG_C
Cuts at: 0 303 1044
Size: 303 741
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 205 300 354 576 660 1044
Size: 103 102 95 54 222 84 384
Fragments arranged by size:
384 222 103 102 95 84 54
BsiEI CG_ry'CG
Cuts at: 0 556 1044
Size: 556 488
BsiHKAI G_wGCw'C
Cuts at: 0 59 1044
Size: 59 985
BsmI GAATG_Cn'
Cuts at: 0 319 1044
Size: 319 725
Bsp1286I G_dGCh'C
Cuts at: 0 59 433 1044
Size: 59 374 611
Fragments arranged by size:
611 374 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 632 722 1044
Size: 632 90 322
Fragments arranged by size:
632 322 90
BsrFI r'CCGG_y
Cuts at: 0 986 1044
Size: 986 58
BstYI r'GATC_y
Cuts at: 0 142 1044
Size: 142 902
BtgI C'CryG_G
Cuts at: 0 596 654 1044
Size: 596 58 390
Fragments arranged by size:
596 390 58
BtsI GCAGTG_nn'
Cuts at: 0 188 1044
Size: 188 856
EaeI y'GGCC_r
Cuts at: 0 393 440 553 1044
Size: 393 47 113 491
Fragments arranged by size:
491 393 113 47
EagI C'GGCC_G
Cuts at: 0 553 1044
Size: 553 491
EarI CTCTTCn'nnn_
Cuts at: 0 11 782 1044
Size: 11 771 262
Fragments arranged by size:
771 262 11
EcoICRI GAG'CTC
Cuts at: 0 57 1044
Size: 57 987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 493 594 1044
Size: 382 111 101 450
Fragments arranged by size:
450 382 111 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 387 1044
Size: 387 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 54 86 181 213 1044
Size: 54 32 95 32 831
Fragments arranged by size:
831 95 54 32 32
HincII GTy'rAC
Cuts at: 0 647 1011 1044
Size: 647 364 33
Fragments arranged by size:
647 364 33
HindIII A'AGCT_T
Cuts at: 0 101 1044
Size: 101 943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1044
Size: 892 152
MscI TGG'CCA
Cuts at: 0 395 442 1044
Size: 395 47 602
Fragments arranged by size:
602 395 47
MslI CAynn'nnrTG
Cuts at: 0 959 1044
Size: 959 85
MspA1I CmG'CkG
Cuts at: 0 567 614 1044
Size: 567 47 430
Fragments arranged by size:
567 430 47
NcoI C'CATG_G
Cuts at: 0 596 654 1044
Size: 596 58 390
Fragments arranged by size:
596 390 58
NsiI A_TGCA'T
Cuts at: 0 319 1044
Size: 319 725
NspI r_CATG'y
Cuts at: 0 321 343 887 1044
Size: 321 22 544 157
Fragments arranged by size:
544 321 157 22
PciI A'CATG_T
Cuts at: 0 883 1044
Size: 883 161
PflMI CCAn_nnn'nTGG
Cuts at: 0 841 1044
Size: 841 203
PfoI T'CCnGG_A
Cuts at: 0 215 1044
Size: 215 829
PstI C_TGCA'G
Cuts at: 0 684 1044
Size: 684 360
PvuII CAG'CTG
Cuts at: 0 567 614 1044
Size: 567 47 430
Fragments arranged by size:
567 430 47
SacI G_AGCT'C
Cuts at: 0 59 1044
Size: 59 985
SfcI C'TryA_G
Cuts at: 0 680 1044
Size: 680 364
SmlI C'TyrA_G
Cuts at: 0 10 58 98 1044
Size: 10 48 40 946
Fragments arranged by size:
946 48 40 10
SspI AAT'ATT
Cuts at: 0 39 904 1044
Size: 39 865 140
Fragments arranged by size:
865 140 39
StyI C'CwwG_G
Cuts at: 0 518 596 654 1044
Size: 518 78 58 390
Fragments arranged by size:
518 390 78 58
TatI w'GTAC_w
Cuts at: 0 320 344 542 631 886 1044
Size: 320 24 198 89 255 158
Fragments arranged by size:
320 255 198 158 89 24
XbaI T'CTAG_A
Cuts at: 0 934 1044
Size: 934 110
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 388 1044
Size: 388 656
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI AseI BanII BclI
BfrBI BlpI Bme1580I BplI BpmI BpuEI BsaI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BstYI BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI
FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PflMI PfoI PstI PvuII
SacI SfcI SmlI SspI StyI TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BglI BglII BmgBI BmrI BmtI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I
XhoI XmaI XmnI ZraI