Restriction map for
pF1KB4524
(Linear) (Six-base) MAPSORT of: KIBB4524.seq Check: 6789 from: 1 to: 3816
KIBB4524.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1806 3816
Size: 1806 2010
AclI AA'CG_TT
Cuts at: 0 879 3816
Size: 879 2937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 575 949 1147 1451 1531 2749 3816
Size: 142 433 374 198 304 80 1218 1067
Fragments arranged by size:
1218 1067 433 374 304 198 142 80
AhdI GACnn_n'nnGTC
Cuts at: 0 3030 3816
Size: 3030 786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1604 3816
Size: 1604 2212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 971 1003 2252 2284 3816
Size: 971 32 1249 32 1532
Fragments arranged by size:
1532 1249 971 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 297 891 1608 3816
Size: 297 594 717 2208
Fragments arranged by size:
2208 717 594 297
ApaI G_GGCC'C
Cuts at: 0 29 3653 3816
Size: 29 3624 163
Fragments arranged by size:
3624 163 29
ApoI r'AATT_y
Cuts at: 0 112 207 2096 3194 3222 3397 3816
Size: 112 95 1889 1098 28 175 419
Fragments arranged by size:
1889 1098 419 175 112 95 28
AseI AT'TA_AT
Cuts at: 0 1390 3816
Size: 1390 2426
AvaI C'yCGr_G
Cuts at: 0 41 3816
Size: 41 3775
BamHI G'GATC_C
Cuts at: 0 744 3816
Size: 744 3072
BanI G'GyrC_C
Cuts at: 0 1364 3816
Size: 1364 2452
BanII G_rGCy'C
Cuts at: 0 29 67 424 3653 3816
Size: 29 38 357 3229 163
Fragments arranged by size:
3229 357 163 38 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 3595 3816
Size: 3595 221
BbvCI CC'TCA_GC
Cuts at: 0 2366 3816
Size: 2366 1450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1030 1064 3816
Size: 1030 34 2752
Fragments arranged by size:
2752 1030 34
BclI T'GATC_A
Cuts at: 0 950 1395 3816
Size: 950 445 2421
Fragments arranged by size:
2421 950 445
BfrBI ATG'CAT
Cuts at: 0 3321 3816
Size: 3321 495
BglII A'GATC_T
Cuts at: 0 1542 3816
Size: 1542 2274
BlpI GC'TnA_GC
Cuts at: 0 484 779 3816
Size: 484 295 3037
Fragments arranged by size:
3037 484 295
Bme1580I G_kGCm'C
Cuts at: 0 29 2785 2792 3653 3816
Size: 29 2756 7 861 163
Fragments arranged by size:
2756 861 163 29 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 2275 3816
Size: 2275 1541
BmtI G_CTAG'C
Cuts at: 0 3700 3816
Size: 3700 116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 2391 3571 3816
Size: 96 2295 1180 245
Fragments arranged by size:
2295 1180 245 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 514 1354 1922 2030 2144 2656 2955
Size: 166 348 840 568 108 114 512 299
Cuts at: 2955 3816
Size: 861
Fragments arranged by size:
861 840 568 512 348 299 166 114
108
Bpu10I CC'TnA_GC
Cuts at: 0 2366 3816
Size: 2366 1450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3088 3802 3816
Size: 3088 714 14
Fragments arranged by size:
3088 714 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 3328 3816
Size: 3328 488
BsaAI yAC'GTr
Cuts at: 0 2902 3816
Size: 2902 914
BsaWI w'CCGG_w
Cuts at: 0 2336 3816
Size: 2336 1480
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 442 472 1701 1731 1806 1809
Size: 358 30 54 30 1229 30 75 3
Cuts at: 1809 1836 1839 1938 1968 3816
Size: 27 3 99 30 1848
Fragments arranged by size:
1848 1229 358 99 75 54 30 30
30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 1811 1814 1817 1820 1823 1826 1829
Size: 334 1477 3 3 3 3 3 3
Cuts at: 1829 1832 1835 1838 1872 2024 2102 2135 2138
Size: 3 3 3 34 152 78 33 3
Cuts at: 2138 2177 2908 3816
Size: 39 731 908
Fragments arranged by size:
1477 908 731 334 152 78 39 34
33 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 616 1625 3228 3351 3816
Size: 616 1009 1603 123 465
Fragments arranged by size:
1603 1009 616 465 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 2350 3701 3816
Size: 1312 1038 1351 115
Fragments arranged by size:
1351 1312 1038 115
BsiEI CG_ry'CG
Cuts at: 0 33 3816
Size: 33 3783
BsiHKAI G_wGCw'C
Cuts at: 0 424 1740 3816
Size: 424 1316 2076
Fragments arranged by size:
2076 1316 424
BsmI GAATG_Cn'
Cuts at: 0 1569 2141 2210 2967 3321 3573 3631 3816
Size: 1569 572 69 757 354 252 58 185
Fragments arranged by size:
1569 757 572 354 252 185 69 58
Bsp1286I G_dGCh'C
Cuts at: 0 29 67 424 1740 2785 2792 3653 3816
Size: 29 38 357 1316 1045 7 861 163
Fragments arranged by size:
1316 1045 861 357 163 38 29 7
BspEI T'CCGG_A
Cuts at: 0 2336 3816
Size: 2336 1480
BspHI T'CATG_A
Cuts at: 0 594 3816
Size: 594 3222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1806 3816
Size: 1806 2010
BsrBI CCG'CTC
Cuts at: 0 3525 3816
Size: 3525 291
BsrDI GCAATG_nn'
Cuts at: 0 247 851 3816
Size: 247 604 2965
Fragments arranged by size:
2965 604 247
BsrFI r'CCGG_y
Cuts at: 0 1361 3816
Size: 1361 2455
BsrGI T'GTAC_A
Cuts at: 0 386 3816
Size: 386 3430
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2361 3798 3816
Size: 2361 1437 18
Fragments arranged by size:
2361 1437 18
BstYI r'GATC_y
Cuts at: 0 744 1542 3408 3816
Size: 744 798 1866 408
Fragments arranged by size:
1866 798 744 408
Bsu36I CC'TnA_GG
Cuts at: 0 1973 3816
Size: 1973 1843
BtgI C'CryG_G
Cuts at: 0 33 740 3725 3816
Size: 33 707 2985 91
Fragments arranged by size:
2985 707 91 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3593 3816
Size: 3593 223
BtsI GCAGTG_nn'
Cuts at: 0 1564 2879 3816
Size: 1564 1315 937
Fragments arranged by size:
1564 1315 937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1841 1876 2060 2095 3762 3797 3816
Size: 1841 35 184 35 1667 35 19
Fragments arranged by size:
1841 1667 184 35 35 35 19
EaeI y'GGCC_r
Cuts at: 0 30 3804 3816
Size: 30 3774 12
Fragments arranged by size:
3774 30 12
EagI C'GGCC_G
Cuts at: 0 30 3816
Size: 30 3786
EarI CTCTTCn'nnn_
Cuts at: 0 602 1103 2004 2162 2827 3538 3637 3816
Size: 602 501 901 158 665 711 99 179
Fragments arranged by size:
901 711 665 602 501 179 158 99
EcoICRI GAG'CTC
Cuts at: 0 422 3816
Size: 422 3394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 166 514 575 949 1147 1354 1451
Size: 142 24 348 61 374 198 207 97
Cuts at: 1451 1531 1922 2030 2144 2656 2749 2955 3816
Size: 80 391 108 114 512 93 206 861
Fragments arranged by size:
861 512 391 374 348 207 206 198
142 114 108 97 93 80 61 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 2377 3343 3816
Size: 316 2061 966 473
Fragments arranged by size:
2061 966 473 316
EcoO109I rG'GnC_Cy
Cuts at: 0 25 38 1044 1605 2203 2370 3649 3650
Size: 25 13 1006 561 598 167 1279 1
Cuts at: 3650 3816
Size: 166
Fragments arranged by size:
1279 1006 598 561 167 166 25 13
1
EcoRI G'AATT_C
Cuts at: 0 2096 3816
Size: 2096 1720
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 442 474 942 974 983 1015
Size: 358 32 52 32 468 32 9 32
Cuts at: 1015 2857 2889 3016 3048 3585 3617 3816
Size: 1842 32 127 32 537 32 199
Fragments arranged by size:
1842 537 468 358 199 127 52 32
32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 0 1475 3816
Size: 1475 2341
HindIII A'AGCT_T
Cuts at: 0 665 1534 2311 2356 3016 3816
Size: 665 869 777 45 660 800
Fragments arranged by size:
869 800 777 665 660 45
HpaI GTT'AAC
Cuts at: 0 1475 3816
Size: 1475 2341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 915 1442 2534 2928 3660 3816
Size: 683 232 527 1092 394 732 156
Fragments arranged by size:
1092 732 683 527 394 232 156
MslI CAynn'nnrTG
Cuts at: 0 1150 1156 3562 3816
Size: 1150 6 2406 254
Fragments arranged by size:
2406 1150 254 6
MspA1I CmG'CkG
Cuts at: 0 35 488 891 948 973 3232 3816
Size: 35 453 403 57 25 2259 584
Fragments arranged by size:
2259 584 453 403 57 35 25
NaeI GCC'GGC
Cuts at: 0 1363 3816
Size: 1363 2453
NcoI C'CATG_G
Cuts at: 0 740 3725 3816
Size: 740 2985 91
Fragments arranged by size:
2985 740 91
NdeI CA'TA_TG
Cuts at: 0 222 3816
Size: 222 3594
NgoMIV G'CCGG_C
Cuts at: 0 1361 3816
Size: 1361 2455
NheI G'CTAG_C
Cuts at: 0 3696 3816
Size: 3696 120
NsiI A_TGCA'T
Cuts at: 0 3323 3816
Size: 3323 493
NspI r_CATG'y
Cuts at: 0 2607 2891 3816
Size: 2607 284 925
Fragments arranged by size:
2607 925 284
PfoI T'CCnGG_A
Cuts at: 0 2169 2196 2633 3816
Size: 2169 27 437 1183
Fragments arranged by size:
2169 1183 437 27
PpuMI rG'GwC_Cy
Cuts at: 0 38 1044 1605 2370 3816
Size: 38 1006 561 765 1446
Fragments arranged by size:
1446 1006 765 561 38
PsiI TTA'TAA
Cuts at: 0 2318 3816
Size: 2318 1498
PspOMI G'GGCC_C
Cuts at: 0 25 3649 3816
Size: 25 3624 167
Fragments arranged by size:
3624 167 25
PstI C_TGCA'G
Cuts at: 0 1244 1446 2277 3620 3816
Size: 1244 202 831 1343 196
Fragments arranged by size:
1343 1244 831 202 196
PvuII CAG'CTG
Cuts at: 0 488 948 3232 3816
Size: 488 460 2284 584
Fragments arranged by size:
2284 584 488 460
SacI G_AGCT'C
Cuts at: 0 424 3816
Size: 424 3392
SacII CC_GC'GG
Cuts at: 0 36 3816
Size: 36 3780
SanDI GG'GwC_CC
Cuts at: 0 38 3816
Size: 38 3778
ScaI AGT'ACT
Cuts at: 0 1288 3361 3816
Size: 1288 2073 455
Fragments arranged by size:
2073 1288 455
SexAI A'CCwGG_T
Cuts at: 0 1764 3816
Size: 1764 2052
SfcI C'TryA_G
Cuts at: 0 943 1240 1442 1492 2273 3616 3816
Size: 943 297 202 50 781 1343 200
Fragments arranged by size:
1343 943 781 297 202 200 50
SmaI CCC'GGG
Cuts at: 0 43 3816
Size: 43 3773
SmlI C'TyrA_G
Cuts at: 0 3103 3781 3816
Size: 3103 678 35
Fragments arranged by size:
3103 678 35
SnaBI TAC'GTA
Cuts at: 0 2902 3816
Size: 2902 914
SphI G_CATG'C
Cuts at: 0 2607 3816
Size: 2607 1209
StyI C'CwwG_G
Cuts at: 0 395 740 3503 3713 3725 3743 3791 3816
Size: 395 345 2763 210 12 18 48 25
Fragments arranged by size:
2763 395 345 210 48 25 18 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 525 3816
Size: 525 3291
TatI w'GTAC_w
Cuts at: 0 386 1286 3359 3816
Size: 386 900 2073 457
Fragments arranged by size:
2073 900 457 386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1770 1802 1809 1841 3816
Size: 1216 32 522 32 7 32 1975
Fragments arranged by size:
1975 1216 522 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 0 2661 3816
Size: 2661 1155
XbaI T'CTAG_A
Cuts at: 0 3606 3816
Size: 3606 210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1680 3816
Size: 1680 2136
XmaI C'CCGG_G
Cuts at: 0 41 3816
Size: 41 3775
XmnI GAAnn'nnTTC
Cuts at: 0 210 1178 3816
Size: 210 968 2638
Fragments arranged by size:
2638 968 210
Enzymes that do cut:
AarI AclI AcuI AhdI AlfI AloI AlwNI ApaI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII
HpaI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NsiI NspI PfoI PpuMI PsiI PspOMI PstI
PvuII SacI SacII SanDI ScaI SexAI SfcI SmaI
SmlI SnaBI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AleI
ApaLI AscI AsiSI AvrII BaeI BbeI BciVI BglI
BmgBI BsaBI BsaHI BsiWI BsmBI BssHII BssSI BstAPI
BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EciI
EcoRV FalI FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MscI NarI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SalI SapI SbfI SfiI SfoI SgfI
SgrAI SpeI SrfI SspI StuI SwaI XhoI ZraI