Restriction map for
pF1KB4505
(Linear) (Six-base) MAPSORT of: KIBB4505.seq Check: 9209 from: 1 to: 3516
KIBB4505.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 453 554 1069 1249 3120 3261 3516
Size: 453 101 515 180 1871 141 255
Fragments arranged by size:
1871 515 453 255 180 141 101
AatII G_ACGT'C
Cuts at: 0 425 1229 3516
Size: 425 804 2287
Fragments arranged by size:
2287 804 425
AccI GT'mk_AC
Cuts at: 0 2453 3516
Size: 2453 1063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 601 1995 2859 3012 3516
Size: 588 13 1394 864 153 504
Fragments arranged by size:
1394 864 588 504 153 13
AflIII A'CryG_T
Cuts at: 0 947 1018 1186 3516
Size: 947 71 168 2330
Fragments arranged by size:
2330 947 168 71
AgeI A'CCGG_T
Cuts at: 0 192 3516
Size: 192 3324
AhdI GACnn_n'nnGTC
Cuts at: 0 282 3516
Size: 282 3234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2369 3516
Size: 2369 1147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 958 990 3516
Size: 958 32 2526
Fragments arranged by size:
2526 958 32
ApaI G_GGCC'C
Cuts at: 0 143 316 769 1253 3516
Size: 143 173 453 484 2263
Fragments arranged by size:
2263 484 453 173 143
ApaLI G'TGCA_C
Cuts at: 0 2344 2521 3516
Size: 2344 177 995
Fragments arranged by size:
2344 995 177
ApoI r'AATT_y
Cuts at: 0 1033 2172 3516
Size: 1033 1139 1344
Fragments arranged by size:
1344 1139 1033
AvaI C'yCGr_G
Cuts at: 0 787 840 1553 1677 3516
Size: 787 53 713 124 1839
Fragments arranged by size:
1839 787 713 124 53
BamHI G'GATC_C
Cuts at: 0 1538 2901 3516
Size: 1538 1363 615
Fragments arranged by size:
1538 1363 615
BanI G'GyrC_C
Cuts at: 0 121 394 1462 1753 1818 3335 3516
Size: 121 273 1068 291 65 1517 181
Fragments arranged by size:
1517 1068 291 273 181 121 65
BanII G_rGCy'C
Cuts at: 0 143 316 533 769 839 848 920 1253
Size: 143 173 217 236 70 9 72 333
Cuts at: 1253 1495 1686 1943 2066 3516
Size: 242 191 257 123 1450
Fragments arranged by size:
1450 333 257 242 236 217 191 173
143 123 72 70 9
BbeI G_GCGC'C
Cuts at: 0 125 1466 3516
Size: 125 1341 2050
Fragments arranged by size:
2050 1341 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 1331 2192 2415 3453 3516
Size: 1331 861 223 1038 63
Fragments arranged by size:
1331 1038 861 223 63
BbvCI CC'TCA_GC
Cuts at: 0 211 3516
Size: 211 3305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3052 3086 3516
Size: 3052 34 430
Fragments arranged by size:
3052 430 34
BglI GCCn_nnn'nGGC
Cuts at: 0 683 1736 2045 2650 2951 3516
Size: 683 1053 309 605 301 565
Fragments arranged by size:
1053 683 605 565 309 301
BlpI GC'TnA_GC
Cuts at: 0 868 3516
Size: 868 2648
Bme1580I G_kGCm'C
Cuts at: 0 143 295 316 769 1253 1406 1785 2348
Size: 143 152 21 453 484 153 379 563
Cuts at: 2348 2525 3516
Size: 177 991
Fragments arranged by size:
991 563 484 453 379 177 153 152
143 21
BmgBI CAC'GTC
Cuts at: 0 852 1187 2544 3516
Size: 852 335 1357 972
Fragments arranged by size:
1357 972 852 335
BmrI ACTGGGnnnn_n'
Cuts at: 0 1167 1393 1680 2943 3156 3516
Size: 1167 226 287 1263 213 360
Fragments arranged by size:
1263 1167 360 287 226 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 363 3516
Size: 363 3153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 328 436 1338 1509 3516
Size: 27 301 108 902 171 2007
Fragments arranged by size:
2007 902 301 171 108 27
Bpu10I CC'TnA_GC
Cuts at: 0 211 1571 3516
Size: 211 1360 1945
Fragments arranged by size:
1945 1360 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 865 3178 3452 3478 3516
Size: 253 612 2313 274 26 38
Fragments arranged by size:
2313 612 274 253 38 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 414 469 1157 1438 3516
Size: 325 89 55 688 281 2078
Fragments arranged by size:
2078 688 325 281 89 55
BsaAI yAC'GTr
Cuts at: 0 492 573 762 1019 2343 3045 3516
Size: 492 81 189 257 1324 702 471
Fragments arranged by size:
1324 702 492 471 257 189 81
BsaHI Gr'CG_yC
Cuts at: 0 122 422 1226 1463 2390 2681 2711 3516
Size: 122 300 804 237 927 291 30 805
Fragments arranged by size:
927 805 804 300 291 237 122 30
BsaWI w'CCGG_w
Cuts at: 0 192 3516
Size: 192 3324
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1135 1165 2380 2410 3516
Size: 1135 30 1215 30 1106
Fragments arranged by size:
1215 1135 1106 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 795 826 1156 1650 2263 3516
Size: 795 31 330 494 613 1253
Fragments arranged by size:
1253 795 613 494 330 31
BseYI C'CCAG_C
Cuts at: 0 40 263 769 816 1202 3447 3516
Size: 40 223 506 47 386 2245 69
Fragments arranged by size:
2245 506 386 223 69 47 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 996 1500 1630 1710 3516
Size: 481 515 504 130 80 1806
Fragments arranged by size:
1806 515 504 481 130 80
BsiHKAI G_wGCw'C
Cuts at: 0 545 839 920 1180 2348 2525 3230 3516
Size: 545 294 81 260 1168 177 705 286
Fragments arranged by size:
1168 705 545 294 286 260 177 81
BsmI GAATG_Cn'
Cuts at: 0 1111 3508 3516
Size: 1111 2397 8
Fragments arranged by size:
2397 1111 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 351 718 1390 2660 3516
Size: 351 367 672 1270 856
Fragments arranged by size:
1270 856 672 367 351
Bsp1286I G_dGCh'C
Cuts at: 0 143 295 316 533 545 769 839 848
Size: 143 152 21 217 12 224 70 9
Cuts at: 848 920 1180 1253 1406 1495 1686 1785 1943
Size: 72 260 73 153 89 191 99 158
Cuts at: 1943 2066 2348 2525 3230 3516
Size: 123 282 177 705 286
Fragments arranged by size:
705 286 282 260 224 217 191 177
158 153 152 143 123 99 89 73
72 70 21 12 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 453 554 1033 1069 1084 1249 3120 3261
Size: 453 101 479 36 15 165 1871 141
Cuts at: 3261 3516
Size: 255
Fragments arranged by size:
1871 479 453 255 165 141 101 36
15
BsrDI GCAATG_nn'
Cuts at: 0 1755 3516
Size: 1755 1761
BsrFI r'CCGG_y
Cuts at: 0 156 192 971 1214 1531 2735 3073 3516
Size: 156 36 779 243 317 1204 338 443
Fragments arranged by size:
1204 779 443 338 317 243 156 36
BsrGI T'GTAC_A
Cuts at: 0 2024 3516
Size: 2024 1492
BssSI C'ACGA_G
Cuts at: 0 804 856 3516
Size: 804 52 2660
Fragments arranged by size:
2660 804 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1241 1424 3516
Size: 1241 183 2092
Fragments arranged by size:
2092 1241 183
BstEII G'GTnAC_C
Cuts at: 0 55 1328 3088 3516
Size: 55 1273 1760 428
Fragments arranged by size:
1760 1273 428 55
BstYI r'GATC_y
Cuts at: 0 372 1538 2901 3516
Size: 372 1166 1363 615
Fragments arranged by size:
1363 1166 615 372
Bsu36I CC'TnA_GG
Cuts at: 0 3232 3516
Size: 3232 284
BtgI C'CryG_G
Cuts at: 0 135 359 1156 1504 1646 2399 3212 3516
Size: 135 224 797 348 142 753 813 304
Fragments arranged by size:
813 797 753 348 304 224 142 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2225 2976 3516
Size: 2225 751 540
Fragments arranged by size:
2225 751 540
BtsI GCAGTG_nn'
Cuts at: 0 448 1851 2139 3516
Size: 448 1403 288 1377
Fragments arranged by size:
1403 1377 448 288
ClaI AT'CG_AT
Cuts at: 0 1478 3516
Size: 1478 2038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1416 1451 2904 2939 3516
Size: 1416 35 1453 35 577
Fragments arranged by size:
1453 1416 577 35 35
DraI TTT'AAA
Cuts at: 0 1038 3516
Size: 1038 2478
DraIII CAC_nnn'GTG
Cuts at: 0 633 1019 1131 3516
Size: 633 386 112 2385
Fragments arranged by size:
2385 633 386 112
EaeI y'GGCC_r
Cuts at: 0 575 969 1437 1482 2601 2670 3516
Size: 575 394 468 45 1119 69 846
Fragments arranged by size:
1119 846 575 468 394 69 45
EarI CTCTTCn'nnn_
Cuts at: 0 2281 3516
Size: 2281 1235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 568 3516
Size: 568 2948
EcoICRI GAG'CTC
Cuts at: 0 837 918 3516
Size: 837 81 2598
Fragments arranged by size:
2598 837 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 328 436 588 601 1338 1509 1995
Size: 27 301 108 152 13 737 171 486
Cuts at: 1995 2859 3012 3516
Size: 864 153 504
Fragments arranged by size:
864 737 504 486 301 171 153 152
108 27 13
EcoO109I rG'GnC_Cy
Cuts at: 0 312 313 766 1199 1249 1250 1709 1767
Size: 312 1 453 433 50 1 459 58
Cuts at: 1767 3412 3516
Size: 1645 104
Fragments arranged by size:
1645 459 453 433 312 104 58 50
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 3516
Size: 264 3252
HaeII r_GCGC'y
Cuts at: 0 125 1466 3516
Size: 125 1341 2050
Fragments arranged by size:
2050 1341 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 683 715 1135 1167 2380 2412
Size: 268 32 383 32 420 32 1213 32
Cuts at: 2412 3001 3033 3516
Size: 589 32 483
Fragments arranged by size:
1213 589 483 420 383 268 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1833 3516
Size: 1833 1683
KasI G'GCGC_C
Cuts at: 0 121 1462 3516
Size: 121 1341 2054
Fragments arranged by size:
2054 1341 121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1688 2663 3516
Size: 374 1314 975 853
Fragments arranged by size:
1314 975 853 374
MscI TGG'CCA
Cuts at: 0 577 3516
Size: 577 2939
MspA1I CmG'CkG
Cuts at: 0 137 361 1010 1487 2324 3516
Size: 137 224 649 477 837 1192
Fragments arranged by size:
1192 837 649 477 224 137
NaeI GCC'GGC
Cuts at: 0 158 2737 3075 3516
Size: 158 2579 338 441
Fragments arranged by size:
2579 441 338 158
NarI GG'CG_CC
Cuts at: 0 122 1463 3516
Size: 122 1341 2053
Fragments arranged by size:
2053 1341 122
NgoMIV G'CCGG_C
Cuts at: 0 156 2735 3073 3516
Size: 156 2579 338 443
Fragments arranged by size:
2579 443 338 156
NspI r_CATG'y
Cuts at: 0 406 748 951 3516
Size: 406 342 203 2565
Fragments arranged by size:
2565 406 342 203
PciI A'CATG_T
Cuts at: 0 947 3516
Size: 947 2569
PflMI CCAn_nnn'nTGG
Cuts at: 0 1131 1480 2176 3516
Size: 1131 349 696 1340
Fragments arranged by size:
1340 1131 696 349
PfoI T'CCnGG_A
Cuts at: 0 830 1541 3516
Size: 830 711 1975
Fragments arranged by size:
1975 830 711
PmlI CAC'GTG
Cuts at: 0 492 1019 3516
Size: 492 527 2497
Fragments arranged by size:
2497 527 492
PpuMI rG'GwC_Cy
Cuts at: 0 1199 1709 1767 3412 3516
Size: 1199 510 58 1645 104
Fragments arranged by size:
1645 1199 510 104 58
PshAI GACnn'nnGTC
Cuts at: 0 2441 3516
Size: 2441 1075
PspOMI G'GGCC_C
Cuts at: 0 139 312 765 1249 3516
Size: 139 173 453 484 2267
Fragments arranged by size:
2267 484 453 173 139
PstI C_TGCA'G
Cuts at: 0 551 3516
Size: 551 2965
PvuII CAG'CTG
Cuts at: 0 1010 3516
Size: 1010 2506
SacI G_AGCT'C
Cuts at: 0 839 920 3516
Size: 839 81 2596
Fragments arranged by size:
2596 839 81
SacII CC_GC'GG
Cuts at: 0 138 362 3516
Size: 138 224 3154
Fragments arranged by size:
3154 224 138
SanDI GG'GwC_CC
Cuts at: 0 1199 3516
Size: 1199 2317
SbfI CC_TGCA'GG
Cuts at: 0 551 3516
Size: 551 2965
SfcI C'TryA_G
Cuts at: 0 480 547 2996 3327 3516
Size: 480 67 2449 331 189
Fragments arranged by size:
2449 480 331 189 67
SfoI GGC'GCC
Cuts at: 0 123 1464 3516
Size: 123 1341 2052
Fragments arranged by size:
2052 1341 123
SgrAI Cr'CCGG_yG
Cuts at: 0 192 3516
Size: 192 3324
SmaI CCC'GGG
Cuts at: 0 1555 1679 3516
Size: 1555 124 1837
Fragments arranged by size:
1837 1555 124
SmlI C'TyrA_G
Cuts at: 0 268 880 3193 3467 3493 3516
Size: 268 612 2313 274 26 23
Fragments arranged by size:
2313 612 274 268 26 23
SnaBI TAC'GTA
Cuts at: 0 3045 3516
Size: 3045 471
StuI AGG'CCT
Cuts at: 0 1594 3516
Size: 1594 1922
StyI C'CwwG_G
Cuts at: 0 1333 2897 3516
Size: 1333 1564 619
Fragments arranged by size:
1564 1333 619
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 987 1771 2263 2554 2932 3516
Size: 21 966 784 492 291 378 584
Fragments arranged by size:
966 784 584 492 378 291 21
TatI w'GTAC_w
Cuts at: 0 2024 3516
Size: 2024 1492
Tth111I GACn'n_nGTC
Cuts at: 0 2419 3516
Size: 2419 1097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 759 2109 3433 3516
Size: 759 1350 1324 83
Fragments arranged by size:
1350 1324 759 83
XmaI C'CCGG_G
Cuts at: 0 1553 1677 3516
Size: 1553 124 1839
Fragments arranged by size:
1839 1553 124
XmnI GAAnn'nnTTC
Cuts at: 0 656 3516
Size: 656 2860
ZraI GAC'GTC
Cuts at: 0 423 1227 3516
Size: 423 804 2289
Fragments arranged by size:
2289 804 423
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII KasI MmeI MscI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SanDI SbfI
SfcI SfoI SgrAI SmaI SmlI SnaBI StuI StyI
TaqII TatI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AleI AlwNI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI
BsaBI BsiEI BsiWI BspEI BspHI BsrBI BssHII BstBI
BstXI BstZ17I DrdI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PpiI PsiI PspXI PsrI PvuI RsrII SalI
SapI ScaI SexAI SfiI SgfI SpeI SphI SrfI
SspI SwaI TstI XbaI XhoI