Restriction map for
pF1KB4489
(Linear) (Six-base) MAPSORT of: KIBB4489.seq Check: 1597 from: 1 to: 1401
KIBB4489.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 228 1401
Size: 228 1173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1376 1401
Size: 340 1036 25
Fragments arranged by size:
1036 340 25
AflIII A'CryG_T
Cuts at: 0 584 753 1401
Size: 584 169 648
Fragments arranged by size:
648 584 169
AleI CACnn'nnGTG
Cuts at: 0 1338 1401
Size: 1338 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 1084 1401
Size: 274 810 317
Fragments arranged by size:
810 317 274
AlwNI CAG_nnn'CTG
Cuts at: 0 932 1136 1179 1344 1401
Size: 932 204 43 165 57
Fragments arranged by size:
932 204 165 57 43
ApaLI G'TGCA_C
Cuts at: 0 603 1006 1401
Size: 603 403 395
Fragments arranged by size:
603 403 395
AvaI C'yCGr_G
Cuts at: 0 183 1401
Size: 183 1218
BanII G_rGCy'C
Cuts at: 0 174 809 1372 1401
Size: 174 635 563 29
Fragments arranged by size:
635 563 174 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1143 1401
Size: 1143 258
BlpI GC'TnA_GC
Cuts at: 0 460 1172 1401
Size: 460 712 229
Fragments arranged by size:
712 460 229
Bme1580I G_kGCm'C
Cuts at: 0 607 1010 1401
Size: 607 403 391
Fragments arranged by size:
607 403 391
BmrI ACTGGGnnnn_n'
Cuts at: 0 588 1179 1401
Size: 588 591 222
Fragments arranged by size:
591 588 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 349 392 1401
Size: 184 165 43 1009
Fragments arranged by size:
1009 184 165 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1401
Size: 1129 272
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 1401
Size: 706 695
BsaWI w'CCGG_w
Cuts at: 0 1241 1401
Size: 1241 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 607 637 1401
Size: 574 30 3 30 764
Fragments arranged by size:
764 574 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 817 1401
Size: 817 584
BseYI C'CCAG_C
Cuts at: 0 193 455 577 809 1107 1129 1401
Size: 193 262 122 232 298 22 272
Fragments arranged by size:
298 272 262 232 193 122 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 545 1090 1401
Size: 478 67 545 311
Fragments arranged by size:
545 478 311 67
BsiEI CG_ry'CG
Cuts at: 0 827 1401
Size: 827 574
BsiHKAI G_wGCw'C
Cuts at: 0 428 559 607 823 890 1010 1401
Size: 428 131 48 216 67 120 391
Fragments arranged by size:
428 391 216 131 120 67 48
Bsp1286I G_dGCh'C
Cuts at: 0 174 428 559 607 809 823 890 1010
Size: 174 254 131 48 202 14 67 120
Cuts at: 1010 1372 1401
Size: 362 29
Fragments arranged by size:
362 254 202 174 131 120 67 48
29 14
BspEI T'CCGG_A
Cuts at: 0 1241 1401
Size: 1241 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 1401
Size: 228 1173
BsrBI CCG'CTC
Cuts at: 0 218 1401
Size: 218 1183
BssSI C'ACGA_G
Cuts at: 0 313 427 1401
Size: 313 114 974
Fragments arranged by size:
974 313 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1304 1401
Size: 1304 97
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1340 1401
Size: 1340 61
BstYI r'GATC_y
Cuts at: 0 297 1124 1401
Size: 297 827 277
Fragments arranged by size:
827 297 277
BtgI C'CryG_G
Cuts at: 0 152 1197 1401
Size: 152 1045 204
Fragments arranged by size:
1045 204 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1264 1401
Size: 1264 137
BtsI GCAGTG_nn'
Cuts at: 0 56 116 1333 1345 1401
Size: 56 60 1217 12 56
Fragments arranged by size:
1217 60 56 56 12
ClaI AT'CG_AT
Cuts at: 0 766 1401
Size: 766 635
EaeI y'GGCC_r
Cuts at: 0 292 470 608 723 1401
Size: 292 178 138 115 678
Fragments arranged by size:
678 292 178 138 115
EarI CTCTTCn'nnn_
Cuts at: 0 34 49 80 1171 1401
Size: 34 15 31 1091 230
Fragments arranged by size:
1091 230 34 31 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 340 349 392 1376 1401
Size: 184 156 9 43 984 25
Fragments arranged by size:
984 184 156 43 25 9
EcoO109I rG'GnC_Cy
Cuts at: 0 255 1401
Size: 255 1146
HaeII r_GCGC'y
Cuts at: 0 291 488 1401
Size: 291 197 913
Fragments arranged by size:
913 291 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 261 289 293 321 642 674 1356 1388
Size: 261 28 4 28 321 32 682 32
Cuts at: 1388 1401
Size: 13
Fragments arranged by size:
682 321 261 32 32 28 28 13
4
HincII GTy'rAC
Cuts at: 0 672 1401
Size: 672 729
MluI A'CGCG_T
Cuts at: 0 584 1401
Size: 584 817
MscI TGG'CCA
Cuts at: 0 472 725 1401
Size: 472 253 676
Fragments arranged by size:
676 472 253
MslI CAynn'nnrTG
Cuts at: 0 1066 1325 1338 1401
Size: 1066 259 13 63
Fragments arranged by size:
1066 259 63 13
MspA1I CmG'CkG
Cuts at: 0 179 327 745 1133 1401
Size: 179 148 418 388 268
Fragments arranged by size:
418 388 268 179 148
NcoI C'CATG_G
Cuts at: 0 152 1401
Size: 152 1249
NspI r_CATG'y
Cuts at: 0 757 1401
Size: 757 644
PciI A'CATG_T
Cuts at: 0 753 1401
Size: 753 648
PstI C_TGCA'G
Cuts at: 0 239 305 1141 1340 1401
Size: 239 66 836 199 61
Fragments arranged by size:
836 239 199 66 61
PvuII CAG'CTG
Cuts at: 0 179 745 1401
Size: 179 566 656
Fragments arranged by size:
656 566 179
SapI GCTCTTCn'nnn_
Cuts at: 0 1171 1401
Size: 1171 230
ScaI AGT'ACT
Cuts at: 0 413 1401
Size: 413 988
SfcI C'TryA_G
Cuts at: 0 235 301 731 1137 1336 1401
Size: 235 66 430 406 199 65
Fragments arranged by size:
430 406 235 199 66 65
SmaI CCC'GGG
Cuts at: 0 185 1401
Size: 185 1216
SmlI C'TyrA_G
Cuts at: 0 1144 1401
Size: 1144 257
StyI C'CwwG_G
Cuts at: 0 152 1401
Size: 152 1249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 591 813 1121 1401
Size: 591 222 308 280
Fragments arranged by size:
591 308 280 222
TatI w'GTAC_w
Cuts at: 0 411 1013 1234 1401
Size: 411 602 221 167
Fragments arranged by size:
602 411 221 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 989 1021 1401
Size: 574 32 383 32 380
Fragments arranged by size:
574 383 380 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 159 1401
Size: 159 1242
XmaI C'CCGG_G
Cuts at: 0 183 1401
Size: 183 1218
XmnI GAAnn'nnTTC
Cuts at: 0 636 928 1401
Size: 636 292 473
Fragments arranged by size:
636 473 292
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaLI AvaI
BanII BbsI BlpI Bme1580I BmrI BpmI BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI ClaI EaeI EarI Eco57MI EcoO109I HaeII
Hin4I HincII MluI MscI MslI MspA1I NcoI NspI
PciI PstI PvuII SapI ScaI SfcI SmaI SmlI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI ZraI