Restriction map for
pF1KB4486
(Linear) (Six-base) MAPSORT of: KIBB4486.seq Check: 629 from: 1 to: 5055
KIBB4486.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2647 5055
Size: 2647 2408
AccI GT'mk_AC
Cuts at: 0 567 2919 3792 4164 5055
Size: 567 2352 873 372 891
Fragments arranged by size:
2352 891 873 567 372
Acc65I G'GTAC_C
Cuts at: 0 2677 4297 4906 5055
Size: 2677 1620 609 149
Fragments arranged by size:
2677 1620 609 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 4076 4426 5055
Size: 685 3391 350 629
Fragments arranged by size:
3391 685 629 350
AflIII A'CryG_T
Cuts at: 0 4967 5055
Size: 4967 88
AhdI GACnn_n'nnGTC
Cuts at: 0 311 5055
Size: 311 4744
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4440 4826 5055
Size: 4440 386 229
Fragments arranged by size:
4440 386 229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2090 2122 2441 2473 2546 2578 5055
Size: 2090 32 319 32 73 32 2477
Fragments arranged by size:
2477 2090 319 73 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 76 1168 5055
Size: 76 1092 3887
Fragments arranged by size:
3887 1092 76
ApaI G_GGCC'C
Cuts at: 0 345 613 709 3767 5055
Size: 345 268 96 3058 1288
Fragments arranged by size:
3058 1288 345 268 96
ApoI r'AATT_y
Cuts at: 0 242 660 929 1244 2156 2732 5055
Size: 242 418 269 315 912 576 2323
Fragments arranged by size:
2323 912 576 418 315 269 242
AvaI C'yCGr_G
Cuts at: 0 218 5055
Size: 218 4837
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3267 3300 5055
Size: 3267 33 1755
Fragments arranged by size:
3267 1755 33
BamHI G'GATC_C
Cuts at: 0 449 1959 2538 2597 2865 5055
Size: 449 1510 579 59 268 2190
Fragments arranged by size:
2190 1510 579 449 268 59
BanI G'GyrC_C
Cuts at: 0 2659 2677 4297 4906 5055
Size: 2659 18 1620 609 149
Fragments arranged by size:
2659 1620 609 149 18
BanII G_rGCy'C
Cuts at: 0 345 613 709 1290 1602 1704 2381 2884
Size: 345 268 96 581 312 102 677 503
Cuts at: 2884 3767 4494 4631 5055
Size: 883 727 137 424
Fragments arranged by size:
883 727 677 581 503 424 345 312
268 137 102 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 307 691 2026 3214 3223 5055
Size: 307 384 1335 1188 9 1832
Fragments arranged by size:
1832 1335 1188 384 307 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 812 2072 2116 3259 5055
Size: 812 1260 44 1143 1796
Fragments arranged by size:
1796 1260 1143 812 44
BfrBI ATG'CAT
Cuts at: 0 4803 5055
Size: 4803 252
BglI GCCn_nnn'nGGC
Cuts at: 0 213 723 1319 1367 1883 2277 2846 5055
Size: 213 510 596 48 516 394 569 2209
Fragments arranged by size:
2209 596 569 516 510 394 213 48
Bme1580I G_kGCm'C
Cuts at: 0 345 613 709 3767 5055
Size: 345 268 96 3058 1288
Fragments arranged by size:
3058 1288 345 268 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 1882 4004 5055
Size: 1882 2122 1051
Fragments arranged by size:
2122 1882 1051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 419 797 815 1121 1145 1226 1349
Size: 83 336 378 18 306 24 81 123
Cuts at: 1349 1367 1460 1496 1586 1595 1688 1718 1865
Size: 18 93 36 90 9 93 30 147
Cuts at: 1865 2153 2339 2612 2693 2945 2981 3161 3356
Size: 288 186 273 81 252 36 180 195
Cuts at: 3356 3470 3797 3904 4049 4115 4196 4691 5055
Size: 114 327 107 145 66 81 495 364
Fragments arranged by size:
495 378 364 336 327 306 288 273
252 195 186 180 147 145 123 114
107 93 93 90 83 81 81 81
66 36 36 30 24 18 18 9
Bpu10I CC'TnA_GC
Cuts at: 0 4827 5055
Size: 4827 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 4169 5031 5055
Size: 338 3831 862 24
Fragments arranged by size:
3831 862 338 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1504 2957 3040 3777 3823 3949 3976 4265
Size: 1504 1453 83 737 46 126 27 289
Cuts at: 4265 5055
Size: 790
Fragments arranged by size:
1504 1453 790 737 289 126 83 46
27
BsaAI yAC'GTr
Cuts at: 0 823 5055
Size: 823 4232
BsaBI GATnn'nnATC
Cuts at: 0 2870 5055
Size: 2870 2185
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1820 1850 3774 3804 3902 3932 4194 4224
Size: 1820 30 1924 30 98 30 262 30
Cuts at: 4224 5055
Size: 831
Fragments arranged by size:
1924 1820 831 262 98 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4356 5055
Size: 4356 699
BseYI C'CCAG_C
Cuts at: 0 1979 5055
Size: 1979 3076
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3718 4274 4712 4832 5055
Size: 3718 556 438 120 223
Fragments arranged by size:
3718 556 438 223 120
BsiEI CG_ry'CG
Cuts at: 0 3940 5055
Size: 3940 1115
BsiHKAI G_wGCw'C
Cuts at: 0 1602 1611 1704 2178 2310 2610 5055
Size: 1602 9 93 474 132 300 2445
Fragments arranged by size:
2445 1602 474 300 132 93 9
BsmI GAATG_Cn'
Cuts at: 0 329 1589 5055
Size: 329 1260 3466
Fragments arranged by size:
3466 1260 329
Bsp1286I G_dGCh'C
Cuts at: 0 345 613 709 1290 1602 1611 1704 2178
Size: 345 268 96 581 312 9 93 474
Cuts at: 2178 2310 2381 2610 2884 3767 4494 4631 5055
Size: 132 71 229 274 883 727 137 424
Fragments arranged by size:
883 727 581 474 424 345 312 274
268 229 137 132 96 93 71 9
BspHI T'CATG_A
Cuts at: 0 4796 5055
Size: 4796 259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2647 5055
Size: 2647 2408
BsrDI GCAATG_nn'
Cuts at: 0 378 4236 5055
Size: 378 3858 819
Fragments arranged by size:
3858 819 378
BsrFI r'CCGG_y
Cuts at: 0 28 3935 5055
Size: 28 3907 1120
Fragments arranged by size:
3907 1120 28
BsrGI T'GTAC_A
Cuts at: 0 4473 5055
Size: 4473 582
BstAPI GCAn_nnn'nTGC
Cuts at: 0 76 5055
Size: 76 4979
BstEII G'GTnAC_C
Cuts at: 0 790 955 2005 2041 3025 5055
Size: 790 165 1050 36 984 2030
Fragments arranged by size:
2030 1050 984 790 165 36
BstYI r'GATC_y
Cuts at: 0 449 1378 1516 1624 1959 2538 2597 2865
Size: 449 929 138 108 335 579 59 268
Cuts at: 2865 3303 3963 4218 5055
Size: 438 660 255 837
Fragments arranged by size:
929 837 660 579 449 438 335 268
255 138 108 59
BstZ17I GTA'TAC
Cuts at: 0 568 2920 5055
Size: 568 2352 2135
Fragments arranged by size:
2352 2135 568
Bsu36I CC'TnA_GG
Cuts at: 0 641 3110 4037 4760 5055
Size: 641 2469 927 723 295
Fragments arranged by size:
2469 927 723 641 295
BtgI C'CryG_G
Cuts at: 0 4302 4494 5055
Size: 4302 192 561
Fragments arranged by size:
4302 561 192
BtsI GCAGTG_nn'
Cuts at: 0 126 493 5055
Size: 126 367 4562
Fragments arranged by size:
4562 367 126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 584 619 5055
Size: 584 35 4436
Fragments arranged by size:
4436 584 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2135 2348 3014 3050 4058 5055
Size: 2135 213 666 36 1008 997
Fragments arranged by size:
2135 1008 997 666 213 36
EaeI y'GGCC_r
Cuts at: 0 918 3603 3933 3937 4143 5055
Size: 918 2685 330 4 206 912
Fragments arranged by size:
2685 918 912 330 206 4
EagI C'GGCC_G
Cuts at: 0 3937 5055
Size: 3937 1118
EarI CTCTTCn'nnn_
Cuts at: 0 4859 5041 5055
Size: 4859 182 14
Fragments arranged by size:
4859 182 14
EcoICRI GAG'CTC
Cuts at: 0 1600 1702 5055
Size: 1600 102 3353
Fragments arranged by size:
3353 1600 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 419 685 797 815 1121 1145 1226
Size: 83 336 266 112 18 306 24 81
Cuts at: 1226 1349 1367 1460 1496 1586 1595 1688 1718
Size: 123 18 93 36 90 9 93 30
Cuts at: 1718 1865 2153 2339 2612 2693 2945 2981 3161
Size: 147 288 186 273 81 252 36 180
Cuts at: 3161 3356 3470 3797 3904 4049 4076 4115 4196
Size: 195 114 327 107 145 27 39 81
Cuts at: 4196 4426 4691 5055
Size: 230 265 364
Fragments arranged by size:
364 336 327 306 288 273 266 265
252 230 195 186 180 147 145 123
114 112 107 93 93 90 83 81
81 81 39 36 36 30 27 24
18 18 9
EcoO109I rG'GnC_Cy
Cuts at: 0 205 214 277 341 342 349 430 439
Size: 205 9 63 64 1 7 81 9
Cuts at: 439 529 610 637 670 705 799 835 1180
Size: 90 81 27 33 35 94 36 345
Cuts at: 1180 1303 1360 1369 1471 1507 1807 1852 1861
Size: 123 57 9 102 36 300 45 9
Cuts at: 1861 1948 2164 2209 2341 2350 2431 2545 2569
Size: 87 216 45 132 9 81 114 24
Cuts at: 2569 2623 2704 2722 2848 3016 3061 3106 3124
Size: 54 81 18 126 168 45 45 18
Cuts at: 3124 3382 3481 3577 3703 3712 3745 3764 3772
Size: 258 99 96 126 9 33 19 8
Cuts at: 3772 3808 4006 4069 4171 4180 4342 4351 5055
Size: 36 198 63 102 9 162 9 704
Fragments arranged by size:
704 345 300 258 216 205 198 168
162 132 126 126 123 114 102 102
99 96 94 90 87 81 81 81
81 64 63 63 57 54 45 45
45 45 36 36 36 35 33 33
27 24 19 18 18 9 9 9
9 9 9 9 9 8 7 1
EcoRI G'AATT_C
Cuts at: 0 242 929 2156 2732 5055
Size: 242 687 1227 576 2323
Fragments arranged by size:
2323 1227 687 576 242
EcoRV GAT'ATC
Cuts at: 0 523 1647 5055
Size: 523 1124 3408
Fragments arranged by size:
3408 1124 523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2013 2326 5055
Size: 2013 313 2729
Fragments arranged by size:
2729 2013 313
FseI GG_CCGG'CC
Cuts at: 0 3939 5055
Size: 3939 1116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 297 329 597 629 810 842 2691 2723
Size: 297 32 268 32 181 32 1849 32
Cuts at: 2723 3774 3806 4088 4120 5055
Size: 1051 32 282 32 935
Fragments arranged by size:
1849 1051 935 297 282 268 181 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1483 2503 3556 5055
Size: 1483 1020 1053 1499
Fragments arranged by size:
1499 1483 1053 1020
KpnI G_GTAC'C
Cuts at: 0 2681 4301 4910 5055
Size: 2681 1620 609 145
Fragments arranged by size:
2681 1620 609 145
MfeI C'AATT_G
Cuts at: 0 578 749 5055
Size: 578 171 4306
Fragments arranged by size:
4306 578 171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2382 4225 5055
Size: 2382 1843 830
Fragments arranged by size:
2382 1843 830
MscI TGG'CCA
Cuts at: 0 920 3605 4145 5055
Size: 920 2685 540 910
Fragments arranged by size:
2685 920 910 540
MslI CAynn'nnrTG
Cuts at: 0 4422 4636 4808 5055
Size: 4422 214 172 247
Fragments arranged by size:
4422 247 214 172
MspA1I CmG'CkG
Cuts at: 0 3532 4030 4523 4711 5055
Size: 3532 498 493 188 344
Fragments arranged by size:
3532 498 493 344 188
NaeI GCC'GGC
Cuts at: 0 30 3937 5055
Size: 30 3907 1118
Fragments arranged by size:
3907 1118 30
NgoMIV G'CCGG_C
Cuts at: 0 28 3935 5055
Size: 28 3907 1120
Fragments arranged by size:
3907 1120 28
NsiI A_TGCA'T
Cuts at: 0 4805 5055
Size: 4805 250
NspI r_CATG'y
Cuts at: 0 4647 4971 5055
Size: 4647 324 84
Fragments arranged by size:
4647 324 84
PasI CC'CwG_GG
Cuts at: 0 445 1813 1840 2968 3271 3760 5055
Size: 445 1368 27 1128 303 489 1295
Fragments arranged by size:
1368 1295 1128 489 445 303 27
PciI A'CATG_T
Cuts at: 0 4967 5055
Size: 4967 88
PflMI CCAn_nnn'nTGG
Cuts at: 0 3565 5055
Size: 3565 1490
PfoI T'CCnGG_A
Cuts at: 0 99 282 291 318 363 435 516 534
Size: 99 183 9 27 45 72 81 18
Cuts at: 534 633 804 1023 1125 1152 1194 1203 1254
Size: 99 171 219 102 27 42 9 51
Cuts at: 1254 1263 1512 1593 1620 1659 1731 2124 2151
Size: 9 249 81 27 39 72 393 27
Cuts at: 2151 2160 2169 2232 2355 2541 2550 2565 2628
Size: 9 9 63 123 186 9 15 63
Cuts at: 2628 2646 2853 3066 3129 3147 3360 3387 3432
Size: 18 207 213 63 18 213 27 45
Cuts at: 3432 3522 3591 3636 3984 4074 4131 4212 4221
Size: 90 69 45 348 90 57 81 9
Cuts at: 4221 5055
Size: 834
Fragments arranged by size:
834 393 348 249 219 213 213 207
186 183 171 123 102 99 99 90
90 81 81 81 72 72 69 63
63 63 57 51 45 45 45 42
39 27 27 27 27 27 18 18
18 15 9 9 9 9 9 9
9
PpuMI rG'GwC_Cy
Cuts at: 0 277 349 430 439 529 637 670 835
Size: 277 72 81 9 90 108 33 165
Cuts at: 835 1180 1507 1807 1861 1948 2164 2341 2350
Size: 345 327 300 54 87 216 177 9
Cuts at: 2350 2431 2545 2569 2623 2704 2722 3016 3061
Size: 81 114 24 54 81 18 294 45
Cuts at: 3061 3577 3703 3745 3772 3808 4006 4069 4171
Size: 516 126 42 27 36 198 63 102
Cuts at: 4171 4180 4342 4351 5055
Size: 9 162 9 704
Fragments arranged by size:
704 516 345 327 300 294 277 216
198 177 165 162 126 114 108 102
90 87 81 81 81 72 63 54
54 45 42 36 33 27 24 18
9 9 9 9
PshAI GACnn'nnGTC
Cuts at: 0 4277 5055
Size: 4277 778
PspOMI G'GGCC_C
Cuts at: 0 341 609 705 3763 5055
Size: 341 268 96 3058 1292
Fragments arranged by size:
3058 1292 341 268 96
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2894 2926 3276 3308 5055
Size: 2894 32 350 32 1747
Fragments arranged by size:
2894 1747 350 32 32
PstI C_TGCA'G
Cuts at: 0 72 121 1173 5055
Size: 72 49 1052 3882
Fragments arranged by size:
3882 1052 72 49
PvuII CAG'CTG
Cuts at: 0 3532 4030 4523 4711 5055
Size: 3532 498 493 188 344
Fragments arranged by size:
3532 498 493 344 188
SacI G_AGCT'C
Cuts at: 0 1602 1704 5055
Size: 1602 102 3351
Fragments arranged by size:
3351 1602 102
SanDI GG'GwC_CC
Cuts at: 0 670 1861 3703 5055
Size: 670 1191 1842 1352
Fragments arranged by size:
1842 1352 1191 670
SexAI A'CCwGG_T
Cuts at: 0 309 471 552 597 1485 1638 1944 2082
Size: 309 162 81 45 888 153 306 138
Cuts at: 2082 2100 2133 2505 2610 2754 2913 3012 3441
Size: 18 33 372 105 144 159 99 429
Cuts at: 3441 3549 3786 3795 4083 4194 5055
Size: 108 237 9 288 111 861
Fragments arranged by size:
888 861 429 372 309 306 288 237
162 159 153 144 138 111 108 105
99 81 45 33 18 9
SfcI C'TryA_G
Cuts at: 0 68 117 1061 1169 2618 2807 3449 4932
Size: 68 49 944 108 1449 189 642 1483
Cuts at: 4932 4952 5055
Size: 20 103
Fragments arranged by size:
1483 1449 944 642 189 108 103 68
49 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 213 1319 1367 1883 2277 2846 5055
Size: 213 1106 48 516 394 569 2209
Fragments arranged by size:
2209 1106 569 516 394 213 48
SmlI C'TyrA_G
Cuts at: 0 353 4184 5010 5055
Size: 353 3831 826 45
Fragments arranged by size:
3831 826 353 45
SphI G_CATG'C
Cuts at: 0 4647 5055
Size: 4647 408
StuI AGG'CCT
Cuts at: 0 548 1835 3356 5055
Size: 548 1287 1521 1699
Fragments arranged by size:
1699 1521 1287 548
StyI C'CwwG_G
Cuts at: 0 380 1401 3768 4176 5055
Size: 380 1021 2367 408 879
Fragments arranged by size:
2367 1021 879 408 380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 188 809 5055
Size: 80 108 621 4246
Fragments arranged by size:
4246 621 108 80
TatI w'GTAC_w
Cuts at: 0 4473 5055
Size: 4473 582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3902 3934 4762 4794 5055
Size: 3902 32 828 32 261
Fragments arranged by size:
3902 828 261 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3788 5055
Size: 3788 1267
XbaI T'CTAG_A
Cuts at: 0 2202 5055
Size: 2202 2853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2688 3090 3613 3775 3928 5055
Size: 2688 402 523 162 153 1127
Fragments arranged by size:
2688 1127 523 402 162 153
XmnI GAAnn'nnTTC
Cuts at: 0 4868 5055
Size: 4868 187
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AloI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbsI BciVI BfrBI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtsI
CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FseI Hin4I HincII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PpuMI PshAI PspOMI PsrI
PstI PvuII SacI SanDI SexAI SfcI SfiI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI ApaLI AscI
AseI AsiSI AvrII BbeI BbvCI BcgI BclI BglII
BlpI BmgBI BmtI BplI BsaHI BsaWI BsiWI BsmBI
BspEI BsrBI BssHII BssSI BstBI BstXI BtgZI ClaI
DraI DraIII EciI EcoNI FspI FspAI HaeII HindIII
HpaI KasI MluI NarI NcoI NdeI NheI NotI
NruI PacI PmeI PmlI PpiI PsiI PspXI PvuI
RsrII SacII SalI SapI SbfI ScaI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XhoI
XmaI ZraI