Restriction map for
pF1KB4448
(Linear) (Six-base) MAPSORT of: KIBB4448.seq Check: 1912 from: 1 to: 519
KIBB4448.orf
With 182 enzymes: SgfI *
May 26, 2015 10:13 ..
Acc65I G'GTAC_C
Cuts at: 0 292 519
Size: 292 227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 519
Size: 184 335
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 519
Size: 415 32 72
Fragments arranged by size:
415 72 32
ApaI G_GGCC'C
Cuts at: 0 48 245 519
Size: 48 197 274
Fragments arranged by size:
274 197 48
AvaI C'yCGr_G
Cuts at: 0 237 519
Size: 237 282
BanI G'GyrC_C
Cuts at: 0 34 292 519
Size: 34 258 227
Fragments arranged by size:
258 227 34
BanII G_rGCy'C
Cuts at: 0 48 245 519
Size: 48 197 274
Fragments arranged by size:
274 197 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 19 60 519
Size: 19 41 459
Fragments arranged by size:
459 41 19
BclI T'GATC_A
Cuts at: 0 491 519
Size: 491 28
BglI GCCn_nnn'nGGC
Cuts at: 0 43 519
Size: 43 476
BlpI GC'TnA_GC
Cuts at: 0 286 481 519
Size: 286 195 38
Fragments arranged by size:
286 195 38
Bme1580I G_kGCm'C
Cuts at: 0 39 48 245 519
Size: 39 9 197 274
Fragments arranged by size:
274 197 39 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 519
Size: 301 218
BsaAI yAC'GTr
Cuts at: 0 489 519
Size: 489 30
BsaHI Gr'CG_yC
Cuts at: 0 218 299 519
Size: 218 81 220
Fragments arranged by size:
220 218 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 519
Size: 319 200
BsmI GAATG_Cn'
Cuts at: 0 112 519
Size: 112 407
Bsp1286I G_dGCh'C
Cuts at: 0 39 48 245 519
Size: 39 9 197 274
Fragments arranged by size:
274 197 39 9
BsrBI CCG'CTC
Cuts at: 0 290 519
Size: 290 229
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 519
Size: 453 66
BstYI r'GATC_y
Cuts at: 0 92 519
Size: 92 427
BtgI C'CryG_G
Cuts at: 0 498 519
Size: 498 21
ClaI AT'CG_AT
Cuts at: 0 449 519
Size: 449 70
EaeI y'GGCC_r
Cuts at: 0 2 519
Size: 2 517
EarI CTCTTCn'nnn_
Cuts at: 0 410 519
Size: 410 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 427 519
Size: 427 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 519
Size: 184 335
EcoO109I rG'GnC_Cy
Cuts at: 0 159 241 519
Size: 159 82 278
Fragments arranged by size:
278 159 82
HaeII r_GCGC'y
Cuts at: 0 481 519
Size: 481 38
KpnI G_GTAC'C
Cuts at: 0 296 519
Size: 296 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 519
Size: 98 421
MscI TGG'CCA
Cuts at: 0 4 519
Size: 4 515
MslI CAynn'nnrTG
Cuts at: 0 121 451 519
Size: 121 330 68
Fragments arranged by size:
330 121 68
PasI CC'CwG_GG
Cuts at: 0 183 519
Size: 183 336
PfoI T'CCnGG_A
Cuts at: 0 95 519
Size: 95 424
PpuMI rG'GwC_Cy
Cuts at: 0 159 519
Size: 159 360
PshAI GACnn'nnGTC
Cuts at: 0 196 519
Size: 196 323
PspOMI G'GGCC_C
Cuts at: 0 44 241 519
Size: 44 197 278
Fragments arranged by size:
278 197 44
StuI AGG'CCT
Cuts at: 0 235 519
Size: 235 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 511 519
Size: 511 8
TatI w'GTAC_w
Cuts at: 0 368 519
Size: 368 151
Enzymes that do cut:
Acc65I AcuI AloI ApaI AvaI BanI BanII BbsI
BclI BglI BlpI Bme1580I BsaI BsaAI BsaHI BseRI
BsmI Bsp1286I BsrBI BstXI BstYI BtgI ClaI EaeI
EarI EciI Eco57MI EcoO109I HaeII KpnI MmeI MscI
MslI PasI PfoI PpuMI PshAI PspOMI StuI TaqII
TatI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BglII BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI MfeI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI