Restriction map for
pF1KB4430
(Linear) (Six-base) MAPSORT of: KIBB4430.seq Check: 4637 from: 1 to: 1701
KIBB4430.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1242 1701
Size: 1242 459
AccI GT'mk_AC
Cuts at: 0 1340 1701
Size: 1340 361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 808 916 1596 1694 1701
Size: 785 23 108 680 98 7
Fragments arranged by size:
785 680 108 98 23 7
AflIII A'CryG_T
Cuts at: 0 52 688 1469 1701
Size: 52 636 781 232
Fragments arranged by size:
781 636 232 52
AleI CACnn'nnGTG
Cuts at: 0 147 745 1445 1701
Size: 147 598 700 256
Fragments arranged by size:
700 598 256 147
AlwNI CAG_nnn'CTG
Cuts at: 0 588 921 1508 1701
Size: 588 333 587 193
Fragments arranged by size:
588 587 333 193
ApaLI G'TGCA_C
Cuts at: 0 1126 1701
Size: 1126 575
ApoI r'AATT_y
Cuts at: 0 154 1302 1496 1701
Size: 154 1148 194 205
Fragments arranged by size:
1148 205 194 154
AvaI C'yCGr_G
Cuts at: 0 1064 1641 1701
Size: 1064 577 60
Fragments arranged by size:
1064 577 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 988 1021 1701
Size: 988 33 680
Fragments arranged by size:
988 680 33
BanII G_rGCy'C
Cuts at: 0 1147 1701
Size: 1147 554
BbsI GAAGACnn'nnnn_
Cuts at: 0 868 946 1682 1701
Size: 868 78 736 19
Fragments arranged by size:
868 736 78 19
BbvCI CC'TCA_GC
Cuts at: 0 767 929 1701
Size: 767 162 772
Fragments arranged by size:
772 767 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 662 1041 1075 1701
Size: 628 34 379 34 626
Fragments arranged by size:
628 626 379 34 34
BclI T'GATC_A
Cuts at: 0 968 1089 1701
Size: 968 121 612
Fragments arranged by size:
968 612 121
BglII A'GATC_T
Cuts at: 0 830 1701
Size: 830 871
Bme1580I G_kGCm'C
Cuts at: 0 1130 1701
Size: 1130 571
BmrI ACTGGGnnnn_n'
Cuts at: 0 535 1587 1701
Size: 535 1052 114
Fragments arranged by size:
1052 535 114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 870 1701
Size: 870 831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1262 1642 1701
Size: 898 364 380 59
Fragments arranged by size:
898 380 364 59
Bpu10I CC'TnA_GC
Cuts at: 0 16 767 929 1701
Size: 16 751 162 772
Fragments arranged by size:
772 751 162 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1701
Size: 1123 578
BsaAI yAC'GTr
Cuts at: 0 689 1701
Size: 689 1012
BsaBI GATnn'nnATC
Cuts at: 0 1120 1701
Size: 1120 581
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 667 697 1701
Size: 667 30 1004
Fragments arranged by size:
1004 667 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 859 946 1662 1675 1701
Size: 859 87 716 13 26
Fragments arranged by size:
859 716 87 26 13
BseYI C'CCAG_C
Cuts at: 0 1028 1047 1501 1559 1701
Size: 1028 19 454 58 142
Fragments arranged by size:
1028 454 142 58 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 149 1701
Size: 19 130 1552
Fragments arranged by size:
1552 130 19
BsiHKAI G_wGCw'C
Cuts at: 0 644 1130 1147 1361 1442 1701
Size: 644 486 17 214 81 259
Fragments arranged by size:
644 486 259 214 81 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1537 1588 1701
Size: 1537 51 113
Fragments arranged by size:
1537 113 51
Bsp1286I G_dGCh'C
Cuts at: 0 644 1130 1147 1361 1442 1701
Size: 644 486 17 214 81 259
Fragments arranged by size:
644 486 259 214 81 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1183 1242 1701
Size: 1046 137 59 459
Fragments arranged by size:
1046 459 137 59
BsrBI CCG'CTC
Cuts at: 0 671 936 1701
Size: 671 265 765
Fragments arranged by size:
765 671 265
BsrDI GCAATG_nn'
Cuts at: 0 435 1701
Size: 435 1266
BsrFI r'CCGG_y
Cuts at: 0 575 607 1701
Size: 575 32 1094
Fragments arranged by size:
1094 575 32
BssSI C'ACGA_G
Cuts at: 0 1156 1701
Size: 1156 545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 657 735 1353 1701
Size: 657 78 618 348
Fragments arranged by size:
657 618 348 78
BstYI r'GATC_y
Cuts at: 0 830 1701
Size: 830 871
BtgI C'CryG_G
Cuts at: 0 1346 1701
Size: 1346 355
BtsI GCAGTG_nn'
Cuts at: 0 1394 1701
Size: 1394 307
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 1701
Size: 109 1592
EaeI y'GGCC_r
Cuts at: 0 28 1701
Size: 28 1673
EcoICRI GAG'CTC
Cuts at: 0 1145 1701
Size: 1145 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 808 898 916 1262 1596 1642 1694
Size: 785 23 90 18 346 334 46 52
Cuts at: 1694 1701
Size: 7
Fragments arranged by size:
785 346 334 90 52 46 23 18
7
EcoO109I rG'GnC_Cy
Cuts at: 0 21 1035 1134 1701
Size: 21 1014 99 567
Fragments arranged by size:
1014 567 99 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 894 1701
Size: 894 807
FspI TGC'GCA
Cuts at: 0 1042 1701
Size: 1042 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 655 667 687 699 865 897 1327 1359
Size: 655 12 20 12 166 32 430 32
Cuts at: 1359 1624 1656 1701
Size: 265 32 45
Fragments arranged by size:
655 430 265 166 45 32 32 32
20 12 12
HincII GTy'rAC
Cuts at: 0 573 1550 1701
Size: 573 977 151
Fragments arranged by size:
977 573 151
HpaI GTT'AAC
Cuts at: 0 573 1701
Size: 573 1128
MluI A'CGCG_T
Cuts at: 0 52 1701
Size: 52 1649
MslI CAynn'nnrTG
Cuts at: 0 147 745 1445 1531 1701
Size: 147 598 700 86 170
Fragments arranged by size:
700 598 170 147 86
MspA1I CmG'CkG
Cuts at: 0 33 1028 1701
Size: 33 995 673
Fragments arranged by size:
995 673 33
NcoI C'CATG_G
Cuts at: 0 1346 1701
Size: 1346 355
NspI r_CATG'y
Cuts at: 0 1021 1701
Size: 1021 680
PflMI CCAn_nnn'nTGG
Cuts at: 0 1508 1599 1701
Size: 1508 91 102
Fragments arranged by size:
1508 102 91
PfoI T'CCnGG_A
Cuts at: 0 656 1294 1701
Size: 656 638 407
Fragments arranged by size:
656 638 407
PmlI CAC'GTG
Cuts at: 0 689 1701
Size: 689 1012
PpuMI rG'GwC_Cy
Cuts at: 0 1035 1134 1701
Size: 1035 99 567
Fragments arranged by size:
1035 567 99
PshAI GACnn'nnGTC
Cuts at: 0 1336 1701
Size: 1336 365
PstI C_TGCA'G
Cuts at: 0 214 1701
Size: 214 1487
PvuII CAG'CTG
Cuts at: 0 1028 1701
Size: 1028 673
SacI G_AGCT'C
Cuts at: 0 1147 1701
Size: 1147 554
SfcI C'TryA_G
Cuts at: 0 210 411 997 1701
Size: 210 201 586 704
Fragments arranged by size:
704 586 210 201
SmaI CCC'GGG
Cuts at: 0 1066 1701
Size: 1066 635
SmlI C'TyrA_G
Cuts at: 0 1138 1701
Size: 1138 563
StyI C'CwwG_G
Cuts at: 0 986 1346 1427 1701
Size: 986 360 81 274
Fragments arranged by size:
986 360 274 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1348 1701
Size: 1348 353
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 453 485 1701
Size: 453 32 1216
Fragments arranged by size:
1216 453 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1302 1701
Size: 1302 399
XmaI C'CCGG_G
Cuts at: 0 1064 1701
Size: 1064 637
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaLI ApoI
AvaI BaeI BanII BbsI BbvCI BcgI BclI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtsI
DrdI EaeI EcoICRI Eco57MI EcoO109I FalI FspI Hin4I
HincII HpaI MluI MslI MspA1I NcoI NspI PflMI
PfoI PmlI PpuMI PshAI PstI PvuII SacI SfcI
SmaI SmlI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BamHI BanI
BbeI BciVI BfrBI BglI BlpI BmgBI BmtI BsaI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EagI EarI EciI EcoNI EcoRI
EcoRV FseI FspAI HaeII HindIII KasI KpnI MfeI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI