Restriction map for
pF1KB4426
(Linear) (Six-base) MAPSORT of: KIBB4426.seq Check: 1589 from: 1 to: 816
KIBB4426.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 0 206 816
Size: 206 610
AclI AA'CG_TT
Cuts at: 0 809 816
Size: 809 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 467 816
Size: 460 7 349
Fragments arranged by size:
460 349 7
AfeI AGC'GCT
Cuts at: 0 217 816
Size: 217 599
AlwNI CAG_nnn'CTG
Cuts at: 0 60 231 816
Size: 60 171 585
Fragments arranged by size:
585 171 60
ApaI G_GGCC'C
Cuts at: 0 571 816
Size: 571 245
ApaLI G'TGCA_C
Cuts at: 0 73 816
Size: 73 743
BanI G'GyrC_C
Cuts at: 0 627 816
Size: 627 189
BanII G_rGCy'C
Cuts at: 0 67 86 365 571 608 816
Size: 67 19 279 206 37 208
Fragments arranged by size:
279 208 206 67 37 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 496 530 816
Size: 496 34 286
Fragments arranged by size:
496 286 34
BglI GCCn_nnn'nGGC
Cuts at: 0 128 816
Size: 128 688
BglII A'GATC_T
Cuts at: 0 404 816
Size: 404 412
Bme1580I G_kGCm'C
Cuts at: 0 77 115 571 632 816
Size: 77 38 456 61 184
Fragments arranged by size:
456 184 77 61 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 477 816
Size: 477 339
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 411 816
Size: 360 51 405
Fragments arranged by size:
405 360 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 816
Size: 382 434
BsaHI Gr'CG_yC
Cuts at: 0 203 816
Size: 203 613
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 373 400 816
Size: 271 102 27 416
Fragments arranged by size:
416 271 102 27
BseYI C'CCAG_C
Cuts at: 0 118 816
Size: 118 698
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 265 521 816
Size: 253 12 256 295
Fragments arranged by size:
295 256 253 12
BsiEI CG_ry'CG
Cuts at: 0 288 816
Size: 288 528
BsiHKAI G_wGCw'C
Cuts at: 0 77 356 608 816
Size: 77 279 252 208
Fragments arranged by size:
279 252 208 77
Bsp1286I G_dGCh'C
Cuts at: 0 67 77 86 115 356 365 571 608
Size: 67 10 9 29 241 9 206 37
Cuts at: 608 632 816
Size: 24 184
Fragments arranged by size:
241 206 184 67 37 29 24 10
9 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 181 653 816
Size: 181 472 163
Fragments arranged by size:
472 181 163
BsrFI r'CCGG_y
Cuts at: 0 48 816
Size: 48 768
BssSI C'ACGA_G
Cuts at: 0 355 816
Size: 355 461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 173 276 816
Size: 173 103 540
Fragments arranged by size:
540 173 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 816
Size: 126 690
BstYI r'GATC_y
Cuts at: 0 404 816
Size: 404 412
BtgI C'CryG_G
Cuts at: 0 14 288 699 816
Size: 14 274 411 117
Fragments arranged by size:
411 274 117 14
BtsI GCAGTG_nn'
Cuts at: 0 75 458 512 691 816
Size: 75 383 54 179 125
Fragments arranged by size:
383 179 125 75 54
EaeI y'GGCC_r
Cuts at: 0 32 182 285 380 702 816
Size: 32 150 103 95 322 114
Fragments arranged by size:
322 150 114 103 95 32
EagI C'GGCC_G
Cuts at: 0 285 816
Size: 285 531
EcoICRI GAG'CTC
Cuts at: 0 606 816
Size: 606 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 467 816
Size: 460 7 349
Fragments arranged by size:
460 349 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 816
Size: 184 632
EcoO109I rG'GnC_Cy
Cuts at: 0 567 568 816
Size: 567 1 248
Fragments arranged by size:
567 248 1
FseI GG_CCGG'CC
Cuts at: 0 52 816
Size: 52 764
HaeII r_GCGC'y
Cuts at: 0 219 816
Size: 219 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 816
Size: 178 32 606
Fragments arranged by size:
606 178 32
HincII GTy'rAC
Cuts at: 0 771 816
Size: 771 45
MfeI C'AATT_G
Cuts at: 0 651 816
Size: 651 165
MspA1I CmG'CkG
Cuts at: 0 16 267 290 816
Size: 16 251 23 526
Fragments arranged by size:
526 251 23 16
NaeI GCC'GGC
Cuts at: 0 50 816
Size: 50 766
NgoMIV G'CCGG_C
Cuts at: 0 48 816
Size: 48 768
PasI CC'CwG_GG
Cuts at: 0 126 225 816
Size: 126 99 591
Fragments arranged by size:
591 126 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 816
Size: 60 756
PshAI GACnn'nnGTC
Cuts at: 0 555 816
Size: 555 261
PspOMI G'GGCC_C
Cuts at: 0 567 816
Size: 567 249
PstI C_TGCA'G
Cuts at: 0 602 816
Size: 602 214
SacI G_AGCT'C
Cuts at: 0 608 816
Size: 608 208
SacII CC_GC'GG
Cuts at: 0 17 291 816
Size: 17 274 525
Fragments arranged by size:
525 274 17
SbfI CC_TGCA'GG
Cuts at: 0 602 816
Size: 602 214
SfcI C'TryA_G
Cuts at: 0 598 816
Size: 598 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 769 801 816
Size: 769 32 15
Fragments arranged by size:
769 32 15
Tth111I GACn'n_nGTC
Cuts at: 0 483 816
Size: 483 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 621 816
Size: 375 246 195
Fragments arranged by size:
375 246 195
Enzymes that do cut:
AccI AclI AcuI AfeI AlwNI ApaI ApaLI BanI
BanII BcgI BglI BglII Bme1580I BmrI BplI BsaI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI EaeI
EagI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
HincII MfeI MspA1I NaeI NgoMIV PasI PflMI PshAI
PspOMI PstI SacI SacII SbfI SfcI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BlpI BmgBI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoRI
EcoRV FalI FspI FspAI HindIII HpaI KasI KpnI
MluI MmeI MscI MslI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PvuI PvuII
RsrII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI XbaI XhoI
XmaI XmnI ZraI