(Linear) (Six-base) MAPSORT of: KIBB4407.seq Check: 6732 from: 1 to: 5229
KIBB4407.orf
With 182 enzymes: SgfI *
January 29, 2016 11:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2566 3100 4146 5229
Size: 2566 534 1046 1083
Fragments arranged by size:
2566 1083 1046 534
AatII G_ACGT'C
Cuts at: 0 3102 5229
Size: 3102 2127
AccI GT'mk_AC
Cuts at: 0 802 5229
Size: 802 4427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 966 1198 1250 1805 2219 3617 4907
Size: 854 112 232 52 555 414 1398 1290
Cuts at: 4907 5229
Size: 322
Fragments arranged by size:
1398 1290 854 555 414 322 232 112
52
AfeI AGC'GCT
Cuts at: 0 5068 5229
Size: 5068 161
AflII C'TTAA_G
Cuts at: 0 382 5229
Size: 382 4847
AflIII A'CryG_T
Cuts at: 0 2863 3766 5229
Size: 2863 903 1463
Fragments arranged by size:
2863 1463 903
AhdI GACnn_n'nnGTC
Cuts at: 0 1629 3843 4479 5229
Size: 1629 2214 636 750
Fragments arranged by size:
2214 1629 750 636
AleI CACnn'nnGTG
Cuts at: 0 4006 5229
Size: 4006 1223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1432 2506 4178 4447 4645 5229
Size: 1432 1074 1672 269 198 584
Fragments arranged by size:
1672 1432 1074 584 269 198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 4627 4659 5229
Size: 97 32 4498 32 570
Fragments arranged by size:
4498 570 97 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 814 2454 2558 2952 4737 5229
Size: 814 1640 104 394 1785 492
Fragments arranged by size:
1785 1640 814 492 394 104
ApaI G_GGCC'C
Cuts at: 0 1516 5112 5229
Size: 1516 3596 117
Fragments arranged by size:
3596 1516 117
ApaLI G'TGCA_C
Cuts at: 0 2612 5229
Size: 2612 2617
ApoI r'AATT_y
Cuts at: 0 232 579 703 749 1283 1768 2571 2812
Size: 232 347 124 46 534 485 803 241
Cuts at: 2812 3284 5229
Size: 472 1945
Fragments arranged by size:
1945 803 534 485 472 347 241 232
124 46
AvaI C'yCGr_G
Cuts at: 0 890 4467 4981 5229
Size: 890 3577 514 248
Fragments arranged by size:
3577 890 514 248
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3876 3909 5229
Size: 3876 33 1320
Fragments arranged by size:
3876 1320 33
BamHI G'GATC_C
Cuts at: 0 2727 5229
Size: 2727 2502
BanI G'GyrC_C
Cuts at: 0 3226 4986 5229
Size: 3226 1760 243
Fragments arranged by size:
3226 1760 243
BanII G_rGCy'C
Cuts at: 0 715 915 1516 3341 3877 4577 4982 5037
Size: 715 200 601 1825 536 700 405 55
Cuts at: 5037 5112 5229
Size: 75 117
Fragments arranged by size:
1825 715 700 601 536 405 200 117
75 55
BbeI G_GCGC'C
Cuts at: 0 4990 5229
Size: 4990 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 2143 4829 5229
Size: 2143 2686 400
Fragments arranged by size:
2686 2143 400
BbvCI CC'TCA_GC
Cuts at: 0 596 3380 4397 5229
Size: 596 2784 1017 832
Fragments arranged by size:
2784 1017 832 596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 688 722 5229
Size: 688 34 4507
Fragments arranged by size:
4507 688 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5178 5229
Size: 5178 51
BclI T'GATC_A
Cuts at: 0 1676 3114 3453 4724 5229
Size: 1676 1438 339 1271 505
Fragments arranged by size:
1676 1438 1271 505 339
BfrBI ATG'CAT
Cuts at: 0 2035 5229
Size: 2035 3194
BglI GCCn_nnn'nGGC
Cuts at: 0 3997 5229
Size: 3997 1232
BglII A'GATC_T
Cuts at: 0 1483 5229
Size: 1483 3746
BlpI GC'TnA_GC
Cuts at: 0 2945 4555 5229
Size: 2945 1610 674
Fragments arranged by size:
2945 1610 674
Bme1580I G_kGCm'C
Cuts at: 0 1516 2013 2616 5112 5229
Size: 1516 497 603 2496 117
Fragments arranged by size:
2496 1516 603 497 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 1451 2403 5229
Size: 1451 952 2826
Fragments arranged by size:
2826 1451 952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4482 5085 5229
Size: 4482 603 144
Fragments arranged by size:
4482 603 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 3518 3873 4046 4246 4342 4540 4561
Size: 929 2589 355 173 200 96 198 21
Cuts at: 4561 4642 4654 4759 4996 5039 5227 5229
Size: 81 12 105 237 43 188 2
Fragments arranged by size:
2589 929 355 237 200 198 188 173
105 96 81 43 21 12 2
Bpu10I CC'TnA_GC
Cuts at: 0 596 1742 3010 3303 3380 4397 5229
Size: 596 1146 1268 293 77 1017 832
Fragments arranged by size:
1268 1146 1017 832 596 293 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 2081 3829 5229
Size: 188 1893 1748 1400
Fragments arranged by size:
1893 1748 1400 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 189 2063 3371 5229
Size: 189 1874 1308 1858
Fragments arranged by size:
1874 1858 1308 189
BsaAI yAC'GTr
Cuts at: 0 1365 5229
Size: 1365 3864
BsaBI GATnn'nnATC
Cuts at: 0 2732 3734 4540 5229
Size: 2732 1002 806 689
Fragments arranged by size:
2732 1002 806 689
BsaHI Gr'CG_yC
Cuts at: 0 398 1336 1429 3099 3580 4505 4987 5042
Size: 398 938 93 1670 481 925 482 55
Cuts at: 5042 5229
Size: 187
Fragments arranged by size:
1670 938 925 482 481 398 187 93
55
BsaWI w'CCGG_w
Cuts at: 0 1137 4587 5229
Size: 1137 3450 642
Fragments arranged by size:
3450 1137 642
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1119 1149 4252 4282 4627 4657 5012 5042
Size: 1119 30 3103 30 345 30 355 30
Cuts at: 5042 5229
Size: 187
Fragments arranged by size:
3103 1119 355 345 187 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 1044 1577 2737 3112 3586 3668 3671
Size: 37 1007 533 1160 375 474 82 3
Cuts at: 3671 3867 4243 4246 4471 4618 4705 4708 4711
Size: 196 376 3 225 147 87 3 3
Cuts at: 4711 4786 4876 4879 5229
Size: 75 90 3 350
Fragments arranged by size:
1160 1007 533 474 376 375 350 225
196 147 90 87 82 75 37 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 914 3648 4019 5229
Size: 914 2734 371 1210
Fragments arranged by size:
2734 1210 914 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1438 2224 2512 2734 2921 3549 4307 5229
Size: 1438 786 288 222 187 628 758 922
Fragments arranged by size:
1438 922 786 758 628 288 222 187
BsiEI CG_ry'CG
Cuts at: 0 15 101 4418 4614 5229
Size: 15 86 4317 196 615
Fragments arranged by size:
4317 615 196 86 15
BsiHKAI G_wGCw'C
Cuts at: 0 715 1606 2386 2616 3341 3877 4577 5229
Size: 715 891 780 230 725 536 700 652
Fragments arranged by size:
891 780 725 715 700 652 536 230
BsmI GAATG_Cn'
Cuts at: 0 257 701 918 5229
Size: 257 444 217 4311
Fragments arranged by size:
4311 444 257 217
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4020 4874 5229
Size: 4020 854 355
Fragments arranged by size:
4020 854 355
Bsp1286I G_dGCh'C
Cuts at: 0 715 915 1516 1606 2013 2386 2616 3341
Size: 715 200 601 90 407 373 230 725
Cuts at: 3341 3877 4577 4982 5037 5112 5229
Size: 536 700 405 55 75 117
Fragments arranged by size:
725 715 700 601 536 407 405 373
230 200 117 90 75 55
BspEI T'CCGG_A
Cuts at: 0 1137 5229
Size: 1137 4092
BspHI T'CATG_A
Cuts at: 0 3179 5229
Size: 3179 2050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1764 2566 2837 3100 4046 4146 4958 5229
Size: 1764 802 271 263 946 100 812 271
Fragments arranged by size:
1764 946 812 802 271 271 263 100
BsrBI CCG'CTC
Cuts at: 0 27 1656 3813 4236 4776 5229
Size: 27 1629 2157 423 540 453
Fragments arranged by size:
2157 1629 540 453 423 27
BsrDI GCAATG_nn'
Cuts at: 0 523 1940 5229
Size: 523 1417 3289
Fragments arranged by size:
3289 1417 523
BsrFI r'CCGG_y
Cuts at: 0 68 1877 3996 4075 4613 4850 5229
Size: 68 1809 2119 79 538 237 379
Fragments arranged by size:
2119 1809 538 379 237 79 68
BsrGI T'GTAC_A
Cuts at: 0 336 5229
Size: 336 4893
BssHII G'CGCG_C
Cuts at: 0 4963 5229
Size: 4963 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 823 3031 4536 5229
Size: 823 2208 1505 693
Fragments arranged by size:
2208 1505 823 693
BstEII G'GTnAC_C
Cuts at: 0 2358 3933 5229
Size: 2358 1575 1296
Fragments arranged by size:
2358 1575 1296
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 1273 1347 1614 2879 4008 5229
Size: 72 1201 74 267 1265 1129 1221
Fragments arranged by size:
1265 1221 1201 1129 267 74 72
BstYI r'GATC_y
Cuts at: 0 810 1483 2640 2727 4565 5011 5229
Size: 810 673 1157 87 1838 446 218
Fragments arranged by size:
1838 1157 810 673 446 218 87
Bsu36I CC'TnA_GG
Cuts at: 0 410 650 872 5229
Size: 410 240 222 4357
Fragments arranged by size:
4357 410 240 222
BtgI C'CryG_G
Cuts at: 0 117 2513 3237 4097 4492 5229
Size: 117 2396 724 860 395 737
Fragments arranged by size:
2396 860 737 724 395 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 870 5229
Size: 870 4359
BtsI GCAGTG_nn'
Cuts at: 0 2675 2703 5229
Size: 2675 28 2526
Fragments arranged by size:
2675 2526 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3179 3214 5229
Size: 3179 35 2015
Fragments arranged by size:
3179 2015 35
DraI TTT'AAA
Cuts at: 0 767 2133 5229
Size: 767 1366 3096
Fragments arranged by size:
3096 1366 767
DraIII CAC_nnn'GTG
Cuts at: 0 4545 5229
Size: 4545 684
EaeI y'GGCC_r
Cuts at: 0 12 38 991 3245 3694 3969 3998 4010
Size: 12 26 953 2254 449 275 29 12
Cuts at: 4010 4363 5229
Size: 353 866
Fragments arranged by size:
2254 953 866 449 353 275 29 26
12 12
EagI C'GGCC_G
Cuts at: 0 12 5229
Size: 12 5217
EarI CTCTTCn'nnn_
Cuts at: 0 502 1021 2195 2314 2326 4645 4746 4793
Size: 502 519 1174 119 12 2319 101 47
Cuts at: 4793 4838 5229
Size: 45 391
Fragments arranged by size:
2319 1174 519 502 391 119 101 47
45 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 45 3575 4034 4116 5229
Size: 45 3530 459 82 1113
Fragments arranged by size:
3530 1113 459 82 45
EcoICRI GAG'CTC
Cuts at: 0 713 3339 3875 4575 5229
Size: 713 2626 536 700 654
Fragments arranged by size:
2626 713 700 654 536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 929 966 1198 1250 1805 2219 3518
Size: 854 75 37 232 52 555 414 1299
Cuts at: 3518 3617 3873 4046 4246 4342 4540 4561 4642
Size: 99 256 173 200 96 198 21 81
Cuts at: 4642 4654 4759 4907 4996 5039 5227 5229
Size: 12 105 148 89 43 188 2
Fragments arranged by size:
1299 854 555 414 256 232 200 198
188 173 148 105 99 96 89 81
75 52 43 37 21 12 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 560 731 4228 5229
Size: 560 171 3497 1001
Fragments arranged by size:
3497 1001 560 171
EcoO109I rG'GnC_Cy
Cuts at: 0 1512 5108 5109 5229
Size: 1512 3596 1 120
Fragments arranged by size:
3596 1512 120 1
EcoRI G'AATT_C
Cuts at: 0 1283 1768 5229
Size: 1283 485 3461
Fragments arranged by size:
3461 1283 485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1086 3799 4477 5229
Size: 1086 2713 678 752
Fragments arranged by size:
2713 1086 752 678
FspI TGC'GCA
Cuts at: 0 4154 4282 4621 4807 5229
Size: 4154 128 339 186 422
Fragments arranged by size:
4154 422 339 186 128
FspAI rTGC'GCAy
Cuts at: 0 4282 5229
Size: 4282 947
HaeII r_GCGC'y
Cuts at: 0 4601 4737 4851 4990 5070 5229
Size: 4601 136 114 139 80 159
Fragments arranged by size:
4601 159 139 136 114 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1243 1275 1615 1647 1895 1927 2394 2426
Size: 1243 32 340 32 248 32 467 32
Cuts at: 2426 3663 3695 3829 3861 4078 4110 4269 4301
Size: 1237 32 134 32 217 32 159 32
Cuts at: 4301 4465 4497 5229
Size: 164 32 732
Fragments arranged by size:
1243 1237 732 467 340 248 217 164
159 134 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1953 5229
Size: 1953 3276
HindIII A'AGCT_T
Cuts at: 0 836 1201 3797 5183 5229
Size: 836 365 2596 1386 46
Fragments arranged by size:
2596 1386 836 365 46
KasI G'GCGC_C
Cuts at: 0 4986 5229
Size: 4986 243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 1916 2059 2699 3129 3955 5010 5229
Size: 1229 687 143 640 430 826 1055 219
Fragments arranged by size:
1229 1055 826 687 640 430 219 143
MscI TGG'CCA
Cuts at: 0 993 3247 3696 5229
Size: 993 2254 449 1533
Fragments arranged by size:
2254 1533 993 449
MslI CAynn'nnrTG
Cuts at: 0 923 1298 1414 2335 4006 5229
Size: 923 375 116 921 1671 1223
Fragments arranged by size:
1671 1223 923 921 375 116
MspA1I CmG'CkG
Cuts at: 0 296 2742 3384 4362 5026 5229
Size: 296 2446 642 978 664 203
Fragments arranged by size:
2446 978 664 642 296 203
NaeI GCC'GGC
Cuts at: 0 4852 5229
Size: 4852 377
NarI GG'CG_CC
Cuts at: 0 4987 5229
Size: 4987 242
NcoI C'CATG_G
Cuts at: 0 117 2513 4097 5229
Size: 117 2396 1584 1132
Fragments arranged by size:
2396 1584 1132 117
NdeI CA'TA_TG
Cuts at: 0 1857 3513 3719 5229
Size: 1857 1656 206 1510
Fragments arranged by size:
1857 1656 1510 206
NgoMIV G'CCGG_C
Cuts at: 0 4850 5229
Size: 4850 379
NsiI A_TGCA'T
Cuts at: 0 2037 5229
Size: 2037 3192
NspI r_CATG'y
Cuts at: 0 292 615 1877 2178 4554 5229
Size: 292 323 1262 301 2376 675
Fragments arranged by size:
2376 1262 675 323 301 292
PflMI CCAn_nnn'nTGG
Cuts at: 0 2764 3243 5229
Size: 2764 479 1986
Fragments arranged by size:
2764 1986 479
PfoI T'CCnGG_A
Cuts at: 0 906 3495 4568 4658 5229
Size: 906 2589 1073 90 571
Fragments arranged by size:
2589 1073 906 571 90
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3907 3939 4627 4659 5229
Size: 3907 32 688 32 570
Fragments arranged by size:
3907 688 570 32 32
PshAI GACnn'nnGTC
Cuts at: 0 1457 2075 5045 5229
Size: 1457 618 2970 184
Fragments arranged by size:
2970 1457 618 184
PsiI TTA'TAA
Cuts at: 0 1810 5229
Size: 1810 3419
PspOMI G'GGCC_C
Cuts at: 0 1512 5108 5229
Size: 1512 3596 121
Fragments arranged by size:
3596 1512 121
PstI C_TGCA'G
Cuts at: 0 819 1403 2281 2741 3090 3111 4394 5229
Size: 819 584 878 460 349 21 1283 835
Fragments arranged by size:
1283 878 835 819 584 460 349 21
PvuI CG_AT'CG
Cuts at: 0 4418 5229
Size: 4418 811
PvuII CAG'CTG
Cuts at: 0 2742 3384 5026 5229
Size: 2742 642 1642 203
Fragments arranged by size:
2742 1642 642 203
RsrII CG'GwC_CG
Cuts at: 0 4590 5229
Size: 4590 639
SacI G_AGCT'C
Cuts at: 0 715 3341 3877 4577 5229
Size: 715 2626 536 700 652
Fragments arranged by size:
2626 715 700 652 536
SapI GCTCTTCn'nnn_
Cuts at: 0 2314 2326 4746 4838 5229
Size: 2314 12 2420 92 391
Fragments arranged by size:
2420 2314 391 92 12
ScaI AGT'ACT
Cuts at: 0 192 5229
Size: 192 5037
SexAI A'CCwGG_T
Cuts at: 0 2871 5229
Size: 2871 2358
SfcI C'TryA_G
Cuts at: 0 806 815 1399 1829 1919 2277 2737 3086
Size: 806 9 584 430 90 358 460 349
Cuts at: 3086 3107 4390 5229
Size: 21 1283 839
Fragments arranged by size:
1283 839 806 584 460 430 358 349
90 21 9
SfoI GGC'GCC
Cuts at: 0 4988 5229
Size: 4988 241
SgrAI Cr'CCGG_yG
Cuts at: 0 4850 5229
Size: 4850 379
SmaI CCC'GGG
Cuts at: 0 892 5229
Size: 892 4337
SmlI C'TyrA_G
Cuts at: 0 203 382 2096 3808 5229
Size: 203 179 1714 1712 1421
Fragments arranged by size:
1714 1712 1421 203 179
SphI G_CATG'C
Cuts at: 0 1877 5229
Size: 1877 3352
SspI AAT'ATT
Cuts at: 0 777 2001 5229
Size: 777 1224 3228
Fragments arranged by size:
3228 1224 777
StuI AGG'CCT
Cuts at: 0 1499 5229
Size: 1499 3730
StyI C'CwwG_G
Cuts at: 0 117 561 1088 2513 3388 4097 4508 5229
Size: 117 444 527 1425 875 709 411 721
Fragments arranged by size:
1425 875 721 709 527 444 411 117
SwaI ATTT'AAAT
Cuts at: 0 2133 5229
Size: 2133 3096
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 115 1101 2081 2377 3854 3921 4841 5229
Size: 115 986 980 296 1477 67 920 388
Fragments arranged by size:
1477 986 980 920 388 296 115 67
TatI w'GTAC_w
Cuts at: 0 190 336 1898 4436 5229
Size: 190 146 1562 2538 793
Fragments arranged by size:
2538 1562 793 190 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1119 1151 2696 2728 3044 3076 4049 4081
Size: 1119 32 1545 32 316 32 973 32
Cuts at: 4081 5229
Size: 1148
Fragments arranged by size:
1545 1148 1119 973 316 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3140 5229
Size: 3140 2089
XbaI T'CTAG_A
Cuts at: 0 1186 2187 5229
Size: 1186 1001 3042
Fragments arranged by size:
3042 1186 1001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1068 4515 5229
Size: 1068 3447 714
Fragments arranged by size:
3447 1068 714
XmaI C'CCGG_G
Cuts at: 0 890 5229
Size: 890 4339
XmnI GAAnn'nnTTC
Cuts at: 0 639 947 3782 5229
Size: 639 308 2835 1447
Fragments arranged by size:
2835 1447 639 308
ZraI GAC'GTC
Cuts at: 0 3100 5229
Size: 3100 2129
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PflMI PfoI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SapI ScaI SexAI SfcI
SfoI SgrAI SmaI SmlI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AscI AseI AsiSI AvrII BmgBI
BmtI BsiWI BssSI BstBI BstZ17I ClaI DrdI EcoRV
FseI HpaI KpnI MfeI MluI NheI NotI NruI
PacI PasI PciI PmeI PmlI PpuMI PspXI PsrI
SacII SalI SanDI SbfI SfiI SgfI SnaBI SpeI
SrfI XhoI