Restriction map for
pF1KB4406
(Linear) (Six-base) MAPSORT of: KIBB4406.seq Check: 5618 from: 1 to: 6642
KIBB4406.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3110 5538 5819 6642
Size: 3110 2428 281 823
Fragments arranged by size:
3110 2428 823 281
AatII G_ACGT'C
Cuts at: 0 2432 6626 6642
Size: 2432 4194 16
Fragments arranged by size:
4194 2432 16
AccI GT'mk_AC
Cuts at: 0 1268 4769 6642
Size: 1268 3501 1873
Fragments arranged by size:
3501 1873 1268
Acc65I G'GTAC_C
Cuts at: 0 4319 5736 6642
Size: 4319 1417 906
Fragments arranged by size:
4319 1417 906
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1342 2713 4666 6280 6642
Size: 1159 183 1371 1953 1614 362
Fragments arranged by size:
1953 1614 1371 1159 362 183
AfeI AGC'GCT
Cuts at: 0 246 2857 4468 4597 6642
Size: 246 2611 1611 129 2045
Fragments arranged by size:
2611 2045 1611 246 129
AflIII A'CryG_T
Cuts at: 0 407 738 917 1556 4252 4447 6185 6278
Size: 407 331 179 639 2696 195 1738 93
Cuts at: 6278 6642
Size: 364
Fragments arranged by size:
2696 1738 639 407 364 331 195 179
93
AleI CACnn'nnGTG
Cuts at: 0 1204 3593 4306 6642
Size: 1204 2389 713 2336
Fragments arranged by size:
2389 2336 1204 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2906 6642
Size: 2906 3736
AlwNI CAG_nnn'CTG
Cuts at: 0 102 1768 3554 3867 6079 6642
Size: 102 1666 1786 313 2212 563
Fragments arranged by size:
2212 1786 1666 563 313 102
ApaI G_GGCC'C
Cuts at: 0 4247 6642
Size: 4247 2395
ApaLI G'TGCA_C
Cuts at: 0 319 6642
Size: 319 6323
ApoI r'AATT_y
Cuts at: 0 440 2110 6248 6642
Size: 440 1670 4138 394
Fragments arranged by size:
4138 1670 440 394
AseI AT'TA_AT
Cuts at: 0 1037 1286 6642
Size: 1037 249 5356
Fragments arranged by size:
5356 1037 249
AvaI C'yCGr_G
Cuts at: 0 259 362 857 2033 2325 6642
Size: 259 103 495 1176 292 4317
Fragments arranged by size:
4317 1176 495 292 259 103
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6172 6205 6642
Size: 6172 33 437
Fragments arranged by size:
6172 437 33
BamHI G'GATC_C
Cuts at: 0 3690 6642
Size: 3690 2952
BanI G'GyrC_C
Cuts at: 0 4319 5736 6420 6642
Size: 4319 1417 684 222
Fragments arranged by size:
4319 1417 684 222
BanII G_rGCy'C
Cuts at: 0 78 266 2640 3011 3818 4247 5022 6642
Size: 78 188 2374 371 807 429 775 1620
Fragments arranged by size:
2374 1620 807 775 429 371 188 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 822 956 1779 3226 4450 4807 6642
Size: 822 134 823 1447 1224 357 1835
Fragments arranged by size:
1835 1447 1224 823 822 357 134
BbvCI CC'TCA_GC
Cuts at: 0 2524 6642
Size: 2524 4118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 330 364 3907 3941 6642
Size: 330 34 3543 34 2701
Fragments arranged by size:
3543 2701 330 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3403 3654 6642
Size: 3403 251 2988
Fragments arranged by size:
3403 2988 251
BclI T'GATC_A
Cuts at: 0 2474 6108 6642
Size: 2474 3634 534
Fragments arranged by size:
3634 2474 534
BfrBI ATG'CAT
Cuts at: 0 6373 6642
Size: 6373 269
BglI GCCn_nnn'nGGC
Cuts at: 0 202 3185 3737 4496 6308 6642
Size: 202 2983 552 759 1812 334
Fragments arranged by size:
2983 1812 759 552 334 202
BglII A'GATC_T
Cuts at: 0 5910 6317 6394 6642
Size: 5910 407 77 248
Fragments arranged by size:
5910 407 248 77
BlpI GC'TnA_GC
Cuts at: 0 1433 2941 6445 6642
Size: 1433 1508 3504 197
Fragments arranged by size:
3504 1508 1433 197
Bme1580I G_kGCm'C
Cuts at: 0 323 2654 3068 3629 4247 6425 6642
Size: 323 2331 414 561 618 2178 217
Fragments arranged by size:
2331 2178 618 561 414 323 217
BmgBI CAC'GTC
Cuts at: 0 4448 5627 6642
Size: 4448 1179 1015
Fragments arranged by size:
4448 1179 1015
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 489 546 1008 2550 2691 2931 3357
Size: 333 156 57 462 1542 141 240 426
Cuts at: 3357 5025 6031 6642
Size: 1668 1006 611
Fragments arranged by size:
1668 1542 1006 611 462 426 333 240
156 141 57
BmtI G_CTAG'C
Cuts at: 0 1547 6642
Size: 1547 5095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1349 1444 1484 1598 1822 2512 2839
Size: 350 999 95 40 114 224 690 327
Cuts at: 2839 4828 4954 5220 6519 6642
Size: 1989 126 266 1299 123
Fragments arranged by size:
1989 1299 999 690 350 327 266 224
126 123 114 95 40
Bpu10I CC'TnA_GC
Cuts at: 0 2524 6642
Size: 2524 4118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 3181 3439 5527 6642
Size: 901 2280 258 2088 1115
Fragments arranged by size:
2280 2088 1115 901 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 987 1622 1699 2681 4804 6642
Size: 987 635 77 982 2123 1838
Fragments arranged by size:
2123 1838 987 982 635 77
BsaAI yAC'GTr
Cuts at: 0 408 4253 4865 6642
Size: 408 3845 612 1777
Fragments arranged by size:
3845 1777 612 408
BsaBI GATnn'nnATC
Cuts at: 0 3689 6642
Size: 3689 2953
BsaHI Gr'CG_yC
Cuts at: 0 2429 6623 6642
Size: 2429 4194 19
Fragments arranged by size:
4194 2429 19
BsaWI w'CCGG_w
Cuts at: 0 230 1466 2106 2183 6485 6642
Size: 230 1236 640 77 4302 157
Fragments arranged by size:
4302 1236 640 230 157 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2204 2231 2234 2261 4543 4573 6517 6547
Size: 2204 27 3 27 2282 30 1944 30
Cuts at: 6547 6642
Size: 95
Fragments arranged by size:
2282 2204 1944 95 30 30 27 27
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 1318 1441 1747 2228 3507 5012 5391
Size: 616 702 123 306 481 1279 1505 379
Cuts at: 5391 6549 6642
Size: 1158 93
Fragments arranged by size:
1505 1279 1158 702 616 481 379 306
123 93
BseYI C'CCAG_C
Cuts at: 0 1932 3638 6354 6540 6642
Size: 1932 1706 2716 186 102
Fragments arranged by size:
2716 1932 1706 186 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 172 1857 2013 4352 5516 6386 6642
Size: 88 84 1685 156 2339 1164 870 256
Fragments arranged by size:
2339 1685 1164 870 256 156 88 84
BsiHKAI G_wGCw'C
Cuts at: 0 78 323 1169 1783 2396 4589 6642
Size: 78 245 846 614 613 2193 2053
Fragments arranged by size:
2193 2053 846 614 613 245 78
BsmI GAATG_Cn'
Cuts at: 0 1478 4435 6642
Size: 1478 2957 2207
Fragments arranged by size:
2957 2207 1478
Bsp1286I G_dGCh'C
Cuts at: 0 78 266 323 1169 1783 2396 2640 2654
Size: 78 188 57 846 614 613 244 14
Cuts at: 2654 3011 3068 3629 3818 4247 4589 5022 6425
Size: 357 57 561 189 429 342 433 1403
Cuts at: 6425 6642
Size: 217
Fragments arranged by size:
1403 846 614 613 561 433 429 357
342 244 217 189 188 78 57 57
14
BspEI T'CCGG_A
Cuts at: 0 230 1466 2106 6642
Size: 230 1236 640 4536
Fragments arranged by size:
4536 1236 640 230
BspHI T'CATG_A
Cuts at: 0 725 6642
Size: 725 5917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3110 4297 5538 5819 6642
Size: 3110 1187 1241 281 823
Fragments arranged by size:
3110 1241 1187 823 281
BsrBI CCG'CTC
Cuts at: 0 228 1914 2832 3515 6642
Size: 228 1686 918 683 3127
Fragments arranged by size:
3127 1686 918 683 228
BsrDI GCAATG_nn'
Cuts at: 0 1271 1652 2894 3060 3723 6642
Size: 1271 381 1242 166 663 2919
Fragments arranged by size:
2919 1271 1242 663 381 166
BsrFI r'CCGG_y
Cuts at: 0 1189 2004 5587 6642
Size: 1189 815 3583 1055
Fragments arranged by size:
3583 1189 1055 815
BsrGI T'GTAC_A
Cuts at: 0 1559 4664 6473 6642
Size: 1559 3105 1809 169
Fragments arranged by size:
3105 1809 1559 169
BssHII G'CGCG_C
Cuts at: 0 116 184 6642
Size: 116 68 6458
Fragments arranged by size:
6458 116 68
BssSI C'ACGA_G
Cuts at: 0 145 2647 6642
Size: 145 2502 3995
Fragments arranged by size:
3995 2502 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 6082 6642
Size: 102 5980 560
Fragments arranged by size:
5980 560 102
BstBI TT'CG_AA
Cuts at: 0 4427 6642
Size: 4427 2215
BstEII G'GTnAC_C
Cuts at: 0 1898 6642
Size: 1898 4744
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2640 3382 5038 6322 6642
Size: 2640 742 1656 1284 320
Fragments arranged by size:
2640 1656 1284 742 320
BstYI r'GATC_y
Cuts at: 0 2103 3690 3951 5910 6317 6394 6642
Size: 2103 1587 261 1959 407 77 248
Fragments arranged by size:
2103 1959 1587 407 261 248 77
Bsu36I CC'TnA_GG
Cuts at: 0 3182 4930 6642
Size: 3182 1748 1712
Fragments arranged by size:
3182 1748 1712
BtgI C'CryG_G
Cuts at: 0 1632 1850 3878 5037 5303 5840 6629 6642
Size: 1632 218 2028 1159 266 537 789 13
Fragments arranged by size:
2028 1632 1159 789 537 266 218 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1035 1497 3335 4422 4646 6642
Size: 1035 462 1838 1087 224 1996
Fragments arranged by size:
1996 1838 1087 1035 462 224
BtsI GCAGTG_nn'
Cuts at: 0 58 402 957 1200 1578 2375 3150 3627
Size: 58 344 555 243 378 797 775 477
Cuts at: 3627 3807 3868 4137 4287 4376 4653 5576 6642
Size: 180 61 269 150 89 277 923 1066
Fragments arranged by size:
1066 923 797 775 555 477 378 344
277 269 243 180 150 89 61 58
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2949 2984 3288 3323 4383 4418 4468 4503
Size: 2949 35 304 35 1060 35 50 35
Cuts at: 4503 6642
Size: 2139
Fragments arranged by size:
2949 2139 1060 304 50 35 35 35
35
DraI TTT'AAA
Cuts at: 0 6143 6246 6642
Size: 6143 103 396
Fragments arranged by size:
6143 396 103
DraIII CAC_nnn'GTG
Cuts at: 0 1248 2745 6642
Size: 1248 1497 3897
Fragments arranged by size:
3897 1497 1248
DrdI GACnn_nn'nnGTC
Cuts at: 0 287 6642
Size: 287 6355
EaeI y'GGCC_r
Cuts at: 0 711 990 1649 2847 4134 4476 4497 6642
Size: 711 279 659 1198 1287 342 21 2145
Fragments arranged by size:
2145 1287 1198 711 659 342 279 21
EarI CTCTTCn'nnn_
Cuts at: 0 593 1771 2261 3215 5650 6642
Size: 593 1178 490 954 2435 992
Fragments arranged by size:
2435 1178 992 954 593 490
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1652 1974 6642
Size: 1652 322 4668
Fragments arranged by size:
4668 1652 322
EcoICRI GAG'CTC
Cuts at: 0 76 6642
Size: 76 6566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1159 1342 1349 1444 1484 1598 1822
Size: 350 809 183 7 95 40 114 224
Cuts at: 1822 2512 2713 2839 4666 4828 4954 5220 6280
Size: 690 201 126 1827 162 126 266 1060
Cuts at: 6280 6519 6642
Size: 239 123
Fragments arranged by size:
1827 1060 809 690 350 266 239 224
201 183 162 126 126 123 114 95
40 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1206 1486 3094 4725 5100 6642
Size: 1206 280 1608 1631 375 1542
Fragments arranged by size:
1631 1608 1542 1206 375 280
EcoO109I rG'GnC_Cy
Cuts at: 0 671 2972 3162 4244 4286 4934 6642
Size: 671 2301 190 1082 42 648 1708
Fragments arranged by size:
2301 1708 1082 671 648 190 42
EcoRV GAT'ATC
Cuts at: 0 5256 5316 6642
Size: 5256 60 1326
Fragments arranged by size:
5256 1326 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 474 895 5490 6155 6642
Size: 474 421 4595 665 487
Fragments arranged by size:
4595 665 487 474 421
HaeII r_GCGC'y
Cuts at: 0 248 1440 2442 2859 4470 4599 6642
Size: 248 1192 1002 417 1611 129 2043
Fragments arranged by size:
2043 1611 1192 1002 417 248 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1307 1339 1376 1408 2231 2263 2308 2340
Size: 1307 32 37 32 823 32 45 32
Cuts at: 2340 2588 2620 2917 2949 3121 3153 3995 4027
Size: 248 32 297 32 172 32 842 32
Cuts at: 4027 5197 5229 6343 6375 6642
Size: 1170 32 1114 32 267
Fragments arranged by size:
1307 1170 1114 842 823 297 267 248
172 45 37 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3699 4658 5145 6642
Size: 3699 959 487 1497
Fragments arranged by size:
3699 1497 959 487
HindIII A'AGCT_T
Cuts at: 0 2504 5289 6642
Size: 2504 2785 1353
Fragments arranged by size:
2785 2504 1353
KpnI G_GTAC'C
Cuts at: 0 4323 5740 6642
Size: 4323 1417 902
Fragments arranged by size:
4323 1417 902
MfeI C'AATT_G
Cuts at: 0 1395 4512 5897 6642
Size: 1395 3117 1385 745
Fragments arranged by size:
3117 1395 1385 745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 2042 2438 2619 3629 4764 5966 6042
Size: 671 1371 396 181 1010 1135 1202 76
Cuts at: 6042 6278 6642
Size: 236 364
Fragments arranged by size:
1371 1202 1135 1010 671 396 364 236
181 76
MscI TGG'CCA
Cuts at: 0 713 2849 4136 6642
Size: 713 2136 1287 2506
Fragments arranged by size:
2506 2136 1287 713
MslI CAynn'nnrTG
Cuts at: 0 271 1204 2779 2888 3016 3593 3767 4306
Size: 271 933 1575 109 128 577 174 539
Cuts at: 4306 5036 5813 6158 6642
Size: 730 777 345 484
Fragments arranged by size:
1575 933 777 730 577 539 484 345
271 174 128 109
MspA1I CmG'CkG
Cuts at: 0 619 690 1826 2532 3642 3728 4274 4693
Size: 619 71 1136 706 1110 86 546 419
Cuts at: 4693 4974 6642
Size: 281 1668
Fragments arranged by size:
1668 1136 1110 706 619 546 419 281
86 71
NaeI GCC'GGC
Cuts at: 0 1191 6642
Size: 1191 5451
NcoI C'CATG_G
Cuts at: 0 3878 5037 5303 6629 6642
Size: 3878 1159 266 1326 13
Fragments arranged by size:
3878 1326 1159 266 13
NdeI CA'TA_TG
Cuts at: 0 4795 6642
Size: 4795 1847
NgoMIV G'CCGG_C
Cuts at: 0 1189 6642
Size: 1189 5453
NheI G'CTAG_C
Cuts at: 0 1543 6642
Size: 1543 5099
NruI TCG'CGA
Cuts at: 0 30 6642
Size: 30 6612
NsiI A_TGCA'T
Cuts at: 0 6375 6642
Size: 6375 267
NspI r_CATG'y
Cuts at: 0 742 921 1979 3507 5506 5818 6189 6642
Size: 742 179 1058 1528 1999 312 371 453
Fragments arranged by size:
1999 1528 1058 742 453 371 312 179
PasI CC'CwG_GG
Cuts at: 0 1410 1659 2633 4985 6642
Size: 1410 249 974 2352 1657
Fragments arranged by size:
2352 1657 1410 974 249
PciI A'CATG_T
Cuts at: 0 738 917 6185 6642
Size: 738 179 5268 457
Fragments arranged by size:
5268 738 457 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 1248 1665 3879 4154 5090 6642
Size: 1248 417 2214 275 936 1552
Fragments arranged by size:
2214 1552 1248 936 417 275
PfoI T'CCnGG_A
Cuts at: 0 1602 4610 5783 6642
Size: 1602 3008 1173 859
Fragments arranged by size:
3008 1602 1173 859
PmlI CAC'GTG
Cuts at: 0 4253 6642
Size: 4253 2389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2260 2292 6642
Size: 2260 32 4350
Fragments arranged by size:
4350 2260 32
PpuMI rG'GwC_Cy
Cuts at: 0 671 2972 3162 4286 4934 6642
Size: 671 2301 190 1124 648 1708
Fragments arranged by size:
2301 1708 1124 671 648 190
PspOMI G'GGCC_C
Cuts at: 0 4243 6642
Size: 4243 2399
PstI C_TGCA'G
Cuts at: 0 257 3121 3256 3496 3550 4279 4648 5571
Size: 257 2864 135 240 54 729 369 923
Cuts at: 5571 6497 6642
Size: 926 145
Fragments arranged by size:
2864 926 923 729 369 257 240 145
135 54
PvuII CAG'CTG
Cuts at: 0 619 690 1826 2532 4693 4974 6642
Size: 619 71 1136 706 2161 281 1668
Fragments arranged by size:
2161 1668 1136 706 619 281 71
SacI G_AGCT'C
Cuts at: 0 78 6642
Size: 78 6564
SanDI GG'GwC_CC
Cuts at: 0 2972 6642
Size: 2972 3670
SapI GCTCTTCn'nnn_
Cuts at: 0 1771 5650 6642
Size: 1771 3879 992
Fragments arranged by size:
3879 1771 992
SexAI A'CCwGG_T
Cuts at: 0 1070 2741 6642
Size: 1070 1671 3901
Fragments arranged by size:
3901 1671 1070
SfcI C'TryA_G
Cuts at: 0 253 3117 3252 3492 3546 4275 4644 4773
Size: 253 2864 135 240 54 729 369 129
Cuts at: 4773 5567 6493 6528 6642
Size: 794 926 35 114
Fragments arranged by size:
2864 926 794 729 369 253 240 135
129 114 54 35
SmaI CCC'GGG
Cuts at: 0 859 2035 6642
Size: 859 1176 4607
Fragments arranged by size:
4607 1176 859
SmlI C'TyrA_G
Cuts at: 0 880 3196 3418 5542 6642
Size: 880 2316 222 2124 1100
Fragments arranged by size:
2316 2124 1100 880 222
SpeI A'CTAG_T
Cuts at: 0 5179 6642
Size: 5179 1463
SspI AAT'ATT
Cuts at: 0 1045 6642
Size: 1045 5597
StuI AGG'CCT
Cuts at: 0 643 1505 3601 4576 4642 6642
Size: 643 862 2096 975 66 2000
Fragments arranged by size:
2096 2000 975 862 643 66
StyI C'CwwG_G
Cuts at: 0 356 1154 1202 1487 1859 2423 3095 3878
Size: 356 798 48 285 372 564 672 783
Cuts at: 3878 5037 5303 6219 6629 6642
Size: 1159 266 916 410 13
Fragments arranged by size:
1159 916 798 783 672 564 410 372
356 285 266 48 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1308 3376 4296 4411 4430 5050 5377 6642
Size: 1308 2068 920 115 19 620 327 1265
Fragments arranged by size:
2068 1308 1265 920 620 327 115 19
TatI w'GTAC_w
Cuts at: 0 838 914 1559 3485 4664 4671 4878 5054
Size: 838 76 645 1926 1179 7 207 176
Cuts at: 5054 6473 6642
Size: 1419 169
Fragments arranged by size:
1926 1419 1179 838 645 207 176 169
76 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1014 1046 2032 2064 2746 2776 2778 2808
Size: 1014 32 986 32 682 30 2 30
Cuts at: 2808 3154 3186 3829 3861 4455 4487 4543 4575
Size: 346 32 643 32 594 32 56 32
Cuts at: 4575 5240 5272 6517 6549 6642
Size: 665 32 1245 32 93
Fragments arranged by size:
1245 1014 986 682 665 643 594 346
93 56 32 32 32 32 32 32
32 32 30 30 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 1524 1857 3351 3997 5472 6261 6642
Size: 654 870 333 1494 646 1475 789 381
Fragments arranged by size:
1494 1475 870 789 654 646 381 333
XmaI C'CCGG_G
Cuts at: 0 857 2033 6642
Size: 857 1176 4609
Fragments arranged by size:
4609 1176 857
XmnI GAAnn'nnTTC
Cuts at: 0 1717 2275 3025 5344 6642
Size: 1717 558 750 2319 1298
Fragments arranged by size:
2319 1717 1298 750 558
ZraI GAC'GTC
Cuts at: 0 2430 6624 6642
Size: 2430 4194 18
Fragments arranged by size:
4194 2430 18
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SexAI SfcI SmaI
SmlI SpeI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AloI AscI AsiSI AvrII
BbeI BplI BsiEI BsiWI BsmBI BstZ17I ClaI EagI
EcoRI FseI FspI FspAI HpaI KasI MluI NarI
NotI PacI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SphI SrfI SwaI Tth111I XbaI XhoI