Restriction map for pF1KB4380
 (Linear) (Six-base) MAPSORT of: KIBB4380.seq  Check: 5667  from: 1  to: 1173



KIBB4380.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1156    1173 
   Size:       1156      17
 
AfeI AGC'GCT

Cuts at:      0     790     994    1173 
   Size:        790     204     179

  Fragments arranged by size: 

                 790     204     179
 
AflIII A'CryG_T

Cuts at:      0     419     881    1173 
   Size:        419     462     292

  Fragments arranged by size: 

                 462     419     292
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1105    1137    1173 
   Size:       1105      32      36

  Fragments arranged by size: 

                1105      36      32
 
ApaI G_GGCC'C

Cuts at:      0      88    1173 
   Size:         88    1085
 
AvaI C'yCGr_G

Cuts at:      0      51      88    1173 
   Size:         51      37    1085

  Fragments arranged by size: 

                1085      51      37
 
BanI G'GyrC_C

Cuts at:      0      13    1144    1173 
   Size:         13    1131      29

  Fragments arranged by size: 

                1131      29      13
 
BanII G_rGCy'C

Cuts at:      0      88     644    1173 
   Size:         88     556     529

  Fragments arranged by size: 

                 556     529      88
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     274     573    1103    1125    1173 
   Size:        274     299     530      22      48

  Fragments arranged by size: 

                 530     299     274      48      22
 
BbvCI CC'TCA_GC

Cuts at:      0    1018    1173 
   Size:       1018     155
 
BglI GCCn_nnn'nGGC

Cuts at:      0     766    1173 
   Size:        766     407
 
BlpI GC'TnA_GC

Cuts at:      0     325     400     451     890    1173 
   Size:        325      75      51     439     283

  Fragments arranged by size: 

                 439     325     283      75      51
 
Bme1580I G_kGCm'C

Cuts at:      0      88     335    1173 
   Size:         88     247     838

  Fragments arranged by size: 

                 838     247      88
 
BmgBI CAC'GTC

Cuts at:      0    1154    1173 
   Size:       1154      19
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1138    1173 
   Size:       1138      35
 
Bpu10I CC'TnA_GC

Cuts at:      0    1018    1173 
   Size:       1018     155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     748    1057    1173 
   Size:        171     577     309     116

  Fragments arranged by size: 

                 577     309     171     116
 
BsaAI yAC'GTr

Cuts at:      0     243     468     729     864    1173 
   Size:        243     225     261     135     309

  Fragments arranged by size: 

                 309     261     243     225     135
 
BsaHI Gr'CG_yC

Cuts at:      0     902    1130    1173 
   Size:        902     228      43

  Fragments arranged by size: 

                 902     228      43
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12      24     130    1057    1173 
   Size:         12      12     106     927     116

  Fragments arranged by size: 

                 927     116     106      12      12
 
BseYI C'CCAG_C

Cuts at:      0      46    1173 
   Size:         46    1127
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889    1134    1173 
   Size:        889     245      39

  Fragments arranged by size: 

                 889     245      39
 
BsiHKAI G_wGCw'C

Cuts at:      0     401     452     734    1173 
   Size:        401      51     282     439

  Fragments arranged by size: 

                 439     401     282      51
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     335     401     452     644     734    1173 
   Size:         88     247      66      51     192      90     439

  Fragments arranged by size: 

                 439     247     192      90      88      66      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1156    1173 
   Size:       1156      17
 
BsrBI CCG'CTC

Cuts at:      0     646    1173 
   Size:        646     527
 
BsrDI GCAATG_nn'

Cuts at:      0     302     737    1173 
   Size:        302     435     436

  Fragments arranged by size: 

                 436     435     302
 
BsrFI r'CCGG_y

Cuts at:      0      94     506     769    1047    1173 
   Size:         94     412     263     278     126

  Fragments arranged by size: 

                 412     278     263     126      94
 
BsrGI T'GTAC_A

Cuts at:      0     674    1173 
   Size:        674     499
 
BssSI C'ACGA_G

Cuts at:      0     394    1173 
   Size:        394     779
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1013    1173 
   Size:       1013     160
 
BtgI C'CryG_G

Cuts at:      0     197     473     515     593     704     905    1037    1173 
   Size:        197     276      42      78     111     201     132     136

  Fragments arranged by size: 

                 276     201     197     136     132     111      78      42
            
 
EaeI y'GGCC_r

Cuts at:      0     272     299     470     518     596    1173 
   Size:        272      27     171      48      78     577

  Fragments arranged by size: 

                 577     272     171      78      48      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1138    1173 
   Size:       1138      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84      85     146    1173 
   Size:         84       1      61    1027

  Fragments arranged by size: 

                1027      84      61       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     825    1173 
   Size:        825     348
 
FspI TGC'GCA

Cuts at:      0     751    1173 
   Size:        751     422
 
FspAI rTGC'GCAy

Cuts at:      0     751    1173 
   Size:        751     422
 
HaeII r_GCGC'y

Cuts at:      0     101     173     792     996    1173 
   Size:        101      72     619     204     177

  Fragments arranged by size: 

                 619     204     177     101      72
 
HincII GTy'rAC

Cuts at:      0     417     879    1053    1173 
   Size:        417     462     174     120

  Fragments arranged by size: 

                 462     417     174     120
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1026    1128    1173 
   Size:       1026     102      45

  Fragments arranged by size: 

                1026     102      45
 
MscI TGG'CCA

Cuts at:      0     301     520    1173 
   Size:        301     219     653

  Fragments arranged by size: 

                 653     301     219
 
MslI CAynn'nnrTG

Cuts at:      0     241     631     811    1173 
   Size:        241     390     180     362

  Fragments arranged by size: 

                 390     362     241     180
 
MspA1I CmG'CkG

Cuts at:      0      59     595     873     918     947    1022    1039    1173 
   Size:         59     536     278      45      29      75      17     134

  Fragments arranged by size: 

                 536     278     134      75      59      45      29      17
            
 
NaeI GCC'GGC

Cuts at:      0      96     508     771    1173 
   Size:         96     412     263     402

  Fragments arranged by size: 

                 412     402     263      96
 
NgoMIV G'CCGG_C

Cuts at:      0      94     506     769    1173 
   Size:         94     412     263     404

  Fragments arranged by size: 

                 412     404     263      94
 
NspI r_CATG'y

Cuts at:      0     423     630    1173 
   Size:        423     207     543

  Fragments arranged by size: 

                 543     423     207
 
PciI A'CATG_T

Cuts at:      0     419    1173 
   Size:        419     754
 
PfoI T'CCnGG_A

Cuts at:      0    1156    1173 
   Size:       1156      17
 
PpuMI rG'GwC_Cy

Cuts at:      0     146    1173 
   Size:        146    1027
 
PspOMI G'GGCC_C

Cuts at:      0      84    1173 
   Size:         84    1089
 
PvuII CAG'CTG

Cuts at:      0      59     873     918     947    1022    1173 
   Size:         59     814      45      29      75     151

  Fragments arranged by size: 

                 814     151      75      59      45      29
 
SacII CC_GC'GG

Cuts at:      0     596    1040    1173 
   Size:        596     444     133

  Fragments arranged by size: 

                 596     444     133
 
SfcI C'TryA_G

Cuts at:      0    1086    1173 
   Size:       1086      87
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     766    1173 
   Size:        766     407
 
SmaI CCC'GGG

Cuts at:      0      53      90    1173 
   Size:         53      37    1083

  Fragments arranged by size: 

                1083      53      37
 
SmlI C'TyrA_G

Cuts at:      0     150     763    1072    1173 
   Size:        150     613     309     101

  Fragments arranged by size: 

                 613     309     150     101
 
SrfI GCCC'GGGC

Cuts at:      0      53    1173 
   Size:         53    1120
 
StyI C'CwwG_G

Cuts at:      0     521    1173 
   Size:        521     652
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     534     912    1173 
   Size:        534     378     261

  Fragments arranged by size: 

                 534     378     261
 
TatI w'GTAC_w

Cuts at:      0     674    1173 
   Size:        674     499
 
Tth111I GACn'n_nGTC

Cuts at:      0     412    1173 
   Size:        412     761
 
XmaI C'CCGG_G

Cuts at:      0      51      88    1173 
   Size:         51      37    1085

  Fragments arranged by size: 

                1085      51      37
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AloI     ApaI     AvaI     BanI    BanII
     BbsI    BbvCI     BglI     BlpI Bme1580I    BmgBI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI     BtgI
     EaeI  Eco57MI EcoO109I     FalI     FspI    FspAI    HaeII   HincII
     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PciI
     PfoI    PpuMI   PspOMI    PvuII    SacII     SfcI     SfiI     SmaI
     SmlI     SrfI     StyI    TaqII     TatI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII     BmrI     BmtI     BplI     BsaI    BsaBI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     XmnI     ZraI
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