(Linear) (Six-base) MAPSORT of: KIBB4380.seq Check: 5667 from: 1 to: 1173 KIBB4380.orf With 182 enzymes: SgfI * February 28, 2007 11:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1156 1173 Size: 1156 17 AfeI AGC'GCT Cuts at: 0 790 994 1173 Size: 790 204 179 Fragments arranged by size: 790 204 179 AflIII A'CryG_T Cuts at: 0 419 881 1173 Size: 419 462 292 Fragments arranged by size: 462 419 292 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1105 1137 1173 Size: 1105 32 36 Fragments arranged by size: 1105 36 32 ApaI G_GGCC'C Cuts at: 0 88 1173 Size: 88 1085 AvaI C'yCGr_G Cuts at: 0 51 88 1173 Size: 51 37 1085 Fragments arranged by size: 1085 51 37 BanI G'GyrC_C Cuts at: 0 13 1144 1173 Size: 13 1131 29 Fragments arranged by size: 1131 29 13 BanII G_rGCy'C Cuts at: 0 88 644 1173 Size: 88 556 529 Fragments arranged by size: 556 529 88 BbsI GAAGACnn'nnnn_ Cuts at: 0 274 573 1103 1125 1173 Size: 274 299 530 22 48 Fragments arranged by size: 530 299 274 48 22 BbvCI CC'TCA_GC Cuts at: 0 1018 1173 Size: 1018 155 BglI GCCn_nnn'nGGC Cuts at: 0 766 1173 Size: 766 407 BlpI GC'TnA_GC Cuts at: 0 325 400 451 890 1173 Size: 325 75 51 439 283 Fragments arranged by size: 439 325 283 75 51 Bme1580I G_kGCm'C Cuts at: 0 88 335 1173 Size: 88 247 838 Fragments arranged by size: 838 247 88 BmgBI CAC'GTC Cuts at: 0 1154 1173 Size: 1154 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1138 1173 Size: 1138 35 Bpu10I CC'TnA_GC Cuts at: 0 1018 1173 Size: 1018 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 171 748 1057 1173 Size: 171 577 309 116 Fragments arranged by size: 577 309 171 116 BsaAI yAC'GTr Cuts at: 0 243 468 729 864 1173 Size: 243 225 261 135 309 Fragments arranged by size: 309 261 243 225 135 BsaHI Gr'CG_yC Cuts at: 0 902 1130 1173 Size: 902 228 43 Fragments arranged by size: 902 228 43 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 12 24 130 1057 1173 Size: 12 12 106 927 116 Fragments arranged by size: 927 116 106 12 12 BseYI C'CCAG_C Cuts at: 0 46 1173 Size: 46 1127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 889 1134 1173 Size: 889 245 39 Fragments arranged by size: 889 245 39 BsiHKAI G_wGCw'C Cuts at: 0 401 452 734 1173 Size: 401 51 282 439 Fragments arranged by size: 439 401 282 51 Bsp1286I G_dGCh'C Cuts at: 0 88 335 401 452 644 734 1173 Size: 88 247 66 51 192 90 439 Fragments arranged by size: 439 247 192 90 88 66 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1156 1173 Size: 1156 17 BsrBI CCG'CTC Cuts at: 0 646 1173 Size: 646 527 BsrDI GCAATG_nn' Cuts at: 0 302 737 1173 Size: 302 435 436 Fragments arranged by size: 436 435 302 BsrFI r'CCGG_y Cuts at: 0 94 506 769 1047 1173 Size: 94 412 263 278 126 Fragments arranged by size: 412 278 263 126 94 BsrGI T'GTAC_A Cuts at: 0 674 1173 Size: 674 499 BssSI C'ACGA_G Cuts at: 0 394 1173 Size: 394 779 BstAPI GCAn_nnn'nTGC Cuts at: 0 1013 1173 Size: 1013 160 BtgI C'CryG_G Cuts at: 0 197 473 515 593 704 905 1037 1173 Size: 197 276 42 78 111 201 132 136 Fragments arranged by size: 276 201 197 136 132 111 78 42 EaeI y'GGCC_r Cuts at: 0 272 299 470 518 596 1173 Size: 272 27 171 48 78 577 Fragments arranged by size: 577 272 171 78 48 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1138 1173 Size: 1138 35 EcoO109I rG'GnC_Cy Cuts at: 0 84 85 146 1173 Size: 84 1 61 1027 Fragments arranged by size: 1027 84 61 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 825 1173 Size: 825 348 FspI TGC'GCA Cuts at: 0 751 1173 Size: 751 422 FspAI rTGC'GCAy Cuts at: 0 751 1173 Size: 751 422 HaeII r_GCGC'y Cuts at: 0 101 173 792 996 1173 Size: 101 72 619 204 177 Fragments arranged by size: 619 204 177 101 72 HincII GTy'rAC Cuts at: 0 417 879 1053 1173 Size: 417 462 174 120 Fragments arranged by size: 462 417 174 120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1026 1128 1173 Size: 1026 102 45 Fragments arranged by size: 1026 102 45 MscI TGG'CCA Cuts at: 0 301 520 1173 Size: 301 219 653 Fragments arranged by size: 653 301 219 MslI CAynn'nnrTG Cuts at: 0 241 631 811 1173 Size: 241 390 180 362 Fragments arranged by size: 390 362 241 180 MspA1I CmG'CkG Cuts at: 0 59 595 873 918 947 1022 1039 1173 Size: 59 536 278 45 29 75 17 134 Fragments arranged by size: 536 278 134 75 59 45 29 17 NaeI GCC'GGC Cuts at: 0 96 508 771 1173 Size: 96 412 263 402 Fragments arranged by size: 412 402 263 96 NgoMIV G'CCGG_C Cuts at: 0 94 506 769 1173 Size: 94 412 263 404 Fragments arranged by size: 412 404 263 94 NspI r_CATG'y Cuts at: 0 423 630 1173 Size: 423 207 543 Fragments arranged by size: 543 423 207 PciI A'CATG_T Cuts at: 0 419 1173 Size: 419 754 PfoI T'CCnGG_A Cuts at: 0 1156 1173 Size: 1156 17 PpuMI rG'GwC_Cy Cuts at: 0 146 1173 Size: 146 1027 PspOMI G'GGCC_C Cuts at: 0 84 1173 Size: 84 1089 PvuII CAG'CTG Cuts at: 0 59 873 918 947 1022 1173 Size: 59 814 45 29 75 151 Fragments arranged by size: 814 151 75 59 45 29 SacII CC_GC'GG Cuts at: 0 596 1040 1173 Size: 596 444 133 Fragments arranged by size: 596 444 133 SfcI C'TryA_G Cuts at: 0 1086 1173 Size: 1086 87 SfiI GGCCn_nnn'nGGCC Cuts at: 0 766 1173 Size: 766 407 SmaI CCC'GGG Cuts at: 0 53 90 1173 Size: 53 37 1083 Fragments arranged by size: 1083 53 37 SmlI C'TyrA_G Cuts at: 0 150 763 1072 1173 Size: 150 613 309 101 Fragments arranged by size: 613 309 150 101 SrfI GCCC'GGGC Cuts at: 0 53 1173 Size: 53 1120 StyI C'CwwG_G Cuts at: 0 521 1173 Size: 521 652 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 534 912 1173 Size: 534 378 261 Fragments arranged by size: 534 378 261 TatI w'GTAC_w Cuts at: 0 674 1173 Size: 674 499 Tth111I GACn'n_nGTC Cuts at: 0 412 1173 Size: 412 761 XmaI C'CCGG_G Cuts at: 0 51 88 1173 Size: 51 37 1085 Fragments arranged by size: 1085 51 37 Enzymes that do cut: AarI AfeI AflIII AloI ApaI AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BtgI EaeI Eco57MI EcoO109I FalI FspI FspAI HaeII HincII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PciI PfoI PpuMI PspOMI PvuII SacII SfcI SfiI SmaI SmlI SrfI StyI TaqII TatI Tth111I XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI BsaI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI Hin4I HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI XbaI XcmI XhoI XmnI ZraI