Restriction map for
pF1KB4375
(Linear) (Six-base) MAPSORT of: KIBB4375.seq Check: 2193 from: 1 to: 1167
KIBB4375.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 1167
Size: 170 997
AccI GT'mk_AC
Cuts at: 0 920 1167
Size: 920 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1167
Size: 461 706
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1062 1167
Size: 1062 105
ApaI G_GGCC'C
Cuts at: 0 90 1167
Size: 90 1077
ApaLI G'TGCA_C
Cuts at: 0 987 1087 1167
Size: 987 100 80
Fragments arranged by size:
987 100 80
ApoI r'AATT_y
Cuts at: 0 408 602 620 1167
Size: 408 194 18 547
Fragments arranged by size:
547 408 194 18
AvaI C'yCGr_G
Cuts at: 0 95 240 728 1167
Size: 95 145 488 439
Fragments arranged by size:
488 439 145 95
BanI G'GyrC_C
Cuts at: 0 244 253 262 389 895 1167
Size: 244 9 9 127 506 272
Fragments arranged by size:
506 272 244 127 9 9
BanII G_rGCy'C
Cuts at: 0 90 1167
Size: 90 1077
BglI GCCn_nnn'nGGC
Cuts at: 0 236 281 1167
Size: 236 45 886
Fragments arranged by size:
886 236 45
Bme1580I G_kGCm'C
Cuts at: 0 90 247 392 991 1091 1167
Size: 90 157 145 599 100 76
Fragments arranged by size:
599 157 145 100 90 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 499 1167
Size: 499 668
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 1167
Size: 210 957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 563 641 676 898 1167
Size: 545 18 78 35 222 269
Fragments arranged by size:
545 269 222 78 35 18
Bpu10I CC'TnA_GC
Cuts at: 0 268 332 1167
Size: 268 64 835
Fragments arranged by size:
835 268 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1039 1167
Size: 659 380 128
Fragments arranged by size:
659 380 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 827 1167
Size: 827 340
BsaHI Gr'CG_yC
Cuts at: 0 1043 1167
Size: 1043 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 1167
Size: 361 30 776
Fragments arranged by size:
776 361 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1042 1167
Size: 1042 125
BseYI C'CCAG_C
Cuts at: 0 1146 1167
Size: 1146 21
BsiHKAI G_wGCw'C
Cuts at: 0 812 991 1091 1167
Size: 812 179 100 76
Fragments arranged by size:
812 179 100 76
BsmBI CGTCTCn'nnnn_
Cuts at: 0 997 1167
Size: 997 170
Bsp1286I G_dGCh'C
Cuts at: 0 90 247 392 812 991 1091 1167
Size: 90 157 145 420 179 100 76
Fragments arranged by size:
420 179 157 145 100 90 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 1167
Size: 170 997
BsrDI GCAATG_nn'
Cuts at: 0 492 1167
Size: 492 675
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1146 1167
Size: 1146 21
BstZ17I GTA'TAC
Cuts at: 0 921 1167
Size: 921 246
BtgI C'CryG_G
Cuts at: 0 49 1167
Size: 49 1118
BtsI GCAGTG_nn'
Cuts at: 0 991 1167
Size: 991 176
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1113 1148 1167
Size: 1113 35 19
Fragments arranged by size:
1113 35 19
DrdI GACnn_nn'nnGTC
Cuts at: 0 650 1167
Size: 650 517
EarI CTCTTCn'nnn_
Cuts at: 0 130 1167
Size: 130 1037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 545 563 641 676 898 1167
Size: 461 84 18 78 35 222 269
Fragments arranged by size:
461 269 222 84 78 35 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1054 1167
Size: 1054 113
EcoO109I rG'GnC_Cy
Cuts at: 0 86 87 538 565 574 583 592 661
Size: 86 1 451 27 9 9 9 69
Cuts at: 661 679 697 1167
Size: 18 18 470
Fragments arranged by size:
470 451 86 69 27 18 18 9
9 9 1
EcoRI G'AATT_C
Cuts at: 0 602 620 1167
Size: 602 18 547
Fragments arranged by size:
602 547 18
HaeII r_GCGC'y
Cuts at: 0 49 1167
Size: 49 1118
MslI CAynn'nnrTG
Cuts at: 0 832 1108 1167
Size: 832 276 59
Fragments arranged by size:
832 276 59
MspA1I CmG'CkG
Cuts at: 0 51 116 745 869 1167
Size: 51 65 629 124 298
Fragments arranged by size:
629 298 124 65 51
PasI CC'CwG_GG
Cuts at: 0 588 1167
Size: 588 579
PfoI T'CCnGG_A
Cuts at: 0 543 561 579 615 624 1167
Size: 543 18 18 36 9 543
Fragments arranged by size:
543 543 36 18 18 9
PpuMI rG'GwC_Cy
Cuts at: 0 538 565 574 583 592 661 679 697
Size: 538 27 9 9 9 69 18 18
Cuts at: 697 1167
Size: 470
Fragments arranged by size:
538 470 69 27 18 18 9 9
9
PspOMI G'GGCC_C
Cuts at: 0 86 1167
Size: 86 1081
PstI C_TGCA'G
Cuts at: 0 42 986 1167
Size: 42 944 181
Fragments arranged by size:
944 181 42
PvuII CAG'CTG
Cuts at: 0 116 745 1167
Size: 116 629 422
Fragments arranged by size:
629 422 116
SacII CC_GC'GG
Cuts at: 0 52 1167
Size: 52 1115
SanDI GG'GwC_CC
Cuts at: 0 592 1167
Size: 592 575
SapI GCTCTTCn'nnn_
Cuts at: 0 130 1167
Size: 130 1037
SexAI A'CCwGG_T
Cuts at: 0 648 1167
Size: 648 519
SfcI C'TryA_G
Cuts at: 0 38 757 909 982 1167
Size: 38 719 152 73 185
Fragments arranged by size:
719 185 152 73 38
SmaI CCC'GGG
Cuts at: 0 97 1167
Size: 97 1070
SmlI C'TyrA_G
Cuts at: 0 674 728 1054 1167
Size: 674 54 326 113
Fragments arranged by size:
674 326 113 54
SrfI GCCC'GGGC
Cuts at: 0 97 1167
Size: 97 1070
StyI C'CwwG_G
Cuts at: 0 231 315 765 842 1167
Size: 231 84 450 77 325
Fragments arranged by size:
450 325 231 84 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1138 1167
Size: 1138 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 361 393 1167
Size: 361 32 774
Fragments arranged by size:
774 361 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 700 954 1167
Size: 700 254 213
Fragments arranged by size:
700 254 213
XhoI C'TCGA_G
Cuts at: 0 728 1167
Size: 728 439
XmaI C'CCGG_G
Cuts at: 0 95 1167
Size: 95 1072
XmnI GAAnn'nnTTC
Cuts at: 0 412 976 1167
Size: 412 564 191
Fragments arranged by size:
564 412 191
Enzymes that do cut:
AarI AccI AcuI AlfI ApaI ApaLI ApoI AvaI
BanI BanII BglI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BstXI BstZ17I BtgI BtsI CspCI
DrdI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII MslI
MspA1I PasI PfoI PpuMI PspOMI PstI PvuII SacII
SanDI SapI SexAI SfcI SmaI SmlI SrfI StyI
TaqII TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI
ClaI DraI DraIII EaeI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI
StuI SwaI TatI Tth111I XbaI ZraI