(Linear) (Six-base) MAPSORT of: KIBB4374.seq  Check: 8365  from: 1  to: 1242



KIBB4374.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AccI GT'mk_AC

Cuts at:      0     781    1242 
   Size:        781     461
 
ApoI r'AATT_y

Cuts at:      0     272     841     884    1002    1237    1242 
   Size:        272     569      43     118     235       5

  Fragments arranged by size: 

                 569     272     235     118      43       5
 
AseI AT'TA_AT

Cuts at:      0     398     452    1242 
   Size:        398      54     790

  Fragments arranged by size: 

                 790     398      54
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     926    1242 
   Size:        926     316
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     868     902    1242 
   Size:        868      34     340

  Fragments arranged by size: 

                 868     340      34
 
BlpI GC'TnA_GC

Cuts at:      0     875    1242 
   Size:        875     367
 
Bme1580I G_kGCm'C

Cuts at:      0     263    1242 
   Size:        263     979
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     379     582    1242 
   Size:        379     203     660

  Fragments arranged by size: 

                 660     379     203
 
BmtI G_CTAG'C

Cuts at:      0    1063    1242 
   Size:       1063     179
 
Bpu10I CC'TnA_GC

Cuts at:      0     721    1242 
   Size:        721     521
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     300    1242 
   Size:        300     942
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      43      73    1108    1138    1242 
   Size:         43      30    1035      30     104

  Fragments arranged by size: 

                1035     104      43      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1080    1237    1242 
   Size:       1080     157       5

  Fragments arranged by size: 

                1080     157       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     502    1242 
   Size:        214     288     740

  Fragments arranged by size: 

                 740     288     214
 
BsmI GAATG_Cn'

Cuts at:      0    1039    1154    1242 
   Size:       1039     115      88

  Fragments arranged by size: 

                1039     115      88
 
Bsp1286I G_dGCh'C

Cuts at:      0     263    1242 
   Size:        263     979
 
BsrDI GCAATG_nn'

Cuts at:      0      16     216    1242 
   Size:         16     200    1026

  Fragments arranged by size: 

                1026     200      16
 
BstBI TT'CG_AA

Cuts at:      0    1043    1242 
   Size:       1043     199
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     369    1242 
   Size:        369     873
 
BstYI r'GATC_y

Cuts at:      0    1170    1242 
   Size:       1170      72
 
Bsu36I CC'TnA_GG

Cuts at:      0     559    1242 
   Size:        559     683
 
BtgI C'CryG_G

Cuts at:      0      47    1166    1242 
   Size:         47    1119      76

  Fragments arranged by size: 

                1119      76      47
 
DraI TTT'AAA

Cuts at:      0     461    1242 
   Size:        461     781
 
EaeI y'GGCC_r

Cuts at:      0     477    1242 
   Size:        477     765
 
EarI CTCTTCn'nnn_

Cuts at:      0      86    1242 
   Size:         86    1156
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      92    1033    1242 
   Size:         92     941     209

  Fragments arranged by size: 

                 941     209      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0      97     801     912    1242 
   Size:         97     704     111     330

  Fragments arranged by size: 

                 704     330     111      97
 
EcoRI G'AATT_C

Cuts at:      0     272    1242 
   Size:        272     970
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     681    1242 
   Size:        681     561
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      43      75      95     127     943     975    1242 
   Size:         43      32      20      32     816      32     267

  Fragments arranged by size: 

                 816     267      43      32      32      32      20
 
HindIII A'AGCT_T

Cuts at:      0     658     686    1107    1242 
   Size:        658      28     421     135

  Fragments arranged by size: 

                 658     421     135      28
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     215    1242 
   Size:        113     102    1027

  Fragments arranged by size: 

                1027     113     102
 
MscI TGG'CCA

Cuts at:      0     479    1242 
   Size:        479     763
 
MslI CAynn'nnrTG

Cuts at:      0     412    1242 
   Size:        412     830
 
NheI G'CTAG_C

Cuts at:      0    1059    1242 
   Size:       1059     183
 
PasI CC'CwG_GG

Cuts at:      0    1029    1242 
   Size:       1029     213
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     120     171    1242 
   Size:        120      51    1071

  Fragments arranged by size: 

                1071     120      51
 
PpuMI rG'GwC_Cy

Cuts at:      0      97     801     912    1242 
   Size:         97     704     111     330

  Fragments arranged by size: 

                 704     330     111      97
 
ScaI AGT'ACT

Cuts at:      0     637    1242 
   Size:        637     605
 
SfcI C'TryA_G

Cuts at:      0     277     341     782    1242 
   Size:        277      64     441     460

  Fragments arranged by size: 

                 460     441     277      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     536     665    1242 
   Size:        536     129     577

  Fragments arranged by size: 

                 577     536     129
 
TatI w'GTAC_w

Cuts at:      0     635    1242 
   Size:        635     607
 

 Enzymes that do cut:

     AccI     ApoI     AseI     BbsI     BcgI     BlpI Bme1580I     BmrI
     BmtI   Bpu10I     BsaI    BsaXI    BseRI     BsgI     BsmI Bsp1286I
    BsrDI    BstBI    BstXI    BstYI   Bsu36I     BtgI     DraI     EaeI
     EarI    EcoNI EcoO109I    EcoRI     FalI    Hin4I  HindIII     MmeI
     MscI     MslI     NheI     PasI    PflMI    PpuMI     ScaI     SfcI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI