Restriction map for
pF1KB4372
(Linear) (Six-base) MAPSORT of: KIBB4372.seq Check: 6310 from: 1 to: 2181
KIBB4372.orf
With 182 enzymes: SgfI *
November 16, 2012 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1288 2181
Size: 1288 893
AccI GT'mk_AC
Cuts at: 0 650 2181
Size: 650 1531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1850 2181
Size: 1085 765 331
Fragments arranged by size:
1085 765 331
AleI CACnn'nnGTG
Cuts at: 0 1642 2181
Size: 1642 539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 147 939 2181
Size: 147 792 1242
Fragments arranged by size:
1242 792 147
AlwNI CAG_nnn'CTG
Cuts at: 0 183 2181
Size: 183 1998
ApoI r'AATT_y
Cuts at: 0 205 832 1585 1997 2047 2181
Size: 205 627 753 412 50 134
Fragments arranged by size:
753 627 412 205 134 50
BanII G_rGCy'C
Cuts at: 0 600 1429 2181
Size: 600 829 752
Fragments arranged by size:
829 752 600
BbsI GAAGACnn'nnnn_
Cuts at: 0 769 2181
Size: 769 1412
BbvCI CC'TCA_GC
Cuts at: 0 583 2181
Size: 583 1598
BglI GCCn_nnn'nGGC
Cuts at: 0 1531 2181
Size: 1531 650
BlpI GC'TnA_GC
Cuts at: 0 176 2181
Size: 176 2005
BmgBI CAC'GTC
Cuts at: 0 840 1660 2181
Size: 840 820 521
Fragments arranged by size:
840 820 521
BmrI ACTGGGnnnn_n'
Cuts at: 0 528 2181
Size: 528 1653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 2181
Size: 604 1577
Bpu10I CC'TnA_GC
Cuts at: 0 583 2181
Size: 583 1598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 2181
Size: 742 1439
BsaBI GATnn'nnATC
Cuts at: 0 1048 2181
Size: 1048 1133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 632 662 1053 1083 2181
Size: 632 30 391 30 1098
Fragments arranged by size:
1098 632 391 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 56 242 245 2181
Size: 56 186 3 1936
Fragments arranged by size:
1936 186 56 3
BseYI C'CCAG_C
Cuts at: 0 996 2181
Size: 996 1185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1471 1620 2181
Size: 1471 149 561
Fragments arranged by size:
1471 561 149
BsiEI CG_ry'CG
Cuts at: 0 1052 2181
Size: 1052 1129
BsiHKAI G_wGCw'C
Cuts at: 0 51 2181
Size: 51 2130
Bsp1286I G_dGCh'C
Cuts at: 0 51 600 1429 2181
Size: 51 549 829 752
Fragments arranged by size:
829 752 549 51
BspHI T'CATG_A
Cuts at: 0 377 423 2181
Size: 377 46 1758
Fragments arranged by size:
1758 377 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 446 1288 2181
Size: 446 842 893
Fragments arranged by size:
893 842 446
BsrBI CCG'CTC
Cuts at: 0 797 2181
Size: 797 1384
BsrDI GCAATG_nn'
Cuts at: 0 469 692 2181
Size: 469 223 1489
Fragments arranged by size:
1489 469 223
BsrFI r'CCGG_y
Cuts at: 0 2096 2181
Size: 2096 85
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 2181
Size: 738 1443
BtgI C'CryG_G
Cuts at: 0 345 866 2181
Size: 345 521 1315
Fragments arranged by size:
1315 521 345
BtsI GCAGTG_nn'
Cuts at: 0 23 1637 2181
Size: 23 1614 544
Fragments arranged by size:
1614 544 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2138 2173 2181
Size: 2138 35 8
Fragments arranged by size:
2138 35 8
DraI TTT'AAA
Cuts at: 0 1370 2181
Size: 1370 811
EaeI y'GGCC_r
Cuts at: 0 1523 2181
Size: 1523 658
EarI CTCTTCn'nnn_
Cuts at: 0 1164 1882 2120 2136 2181
Size: 1164 718 238 16 45
Fragments arranged by size:
1164 718 238 45 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1085 1850 2181
Size: 604 481 765 331
Fragments arranged by size:
765 604 481 331
EcoO109I rG'GnC_Cy
Cuts at: 0 871 2181
Size: 871 1310
EcoRV GAT'ATC
Cuts at: 0 213 361 1113 1269 2181
Size: 213 148 752 156 912
Fragments arranged by size:
912 752 213 156 148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 436 2181
Size: 436 1745
FspI TGC'GCA
Cuts at: 0 892 2181
Size: 892 1289
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 295 324 327 632 664 1163 1195
Size: 292 3 29 3 305 32 499 32
Cuts at: 1195 1210 1225 1242 1257 1302 1334 1644 1676
Size: 15 15 17 15 45 32 310 32
Cuts at: 1676 2181
Size: 505
Fragments arranged by size:
505 499 310 305 292 45 32 32
32 32 29 17 15 15 15 3
3
HincII GTy'rAC
Cuts at: 0 150 1835 2181
Size: 150 1685 346
Fragments arranged by size:
1685 346 150
HpaI GTT'AAC
Cuts at: 0 150 1835 2181
Size: 150 1685 346
Fragments arranged by size:
1685 346 150
MfeI C'AATT_G
Cuts at: 0 558 644 2181
Size: 558 86 1537
Fragments arranged by size:
1537 558 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 365 1096 1545 2181
Size: 18 347 731 449 636
Fragments arranged by size:
731 636 449 347 18
MscI TGG'CCA
Cuts at: 0 1525 2181
Size: 1525 656
MslI CAynn'nnrTG
Cuts at: 0 350 428 671 1642 2181
Size: 350 78 243 971 539
Fragments arranged by size:
971 539 350 243 78
MspA1I CmG'CkG
Cuts at: 0 2058 2181
Size: 2058 123
NaeI GCC'GGC
Cuts at: 0 2098 2181
Size: 2098 83
NcoI C'CATG_G
Cuts at: 0 345 2181
Size: 345 1836
NgoMIV G'CCGG_C
Cuts at: 0 2096 2181
Size: 2096 85
NspI r_CATG'y
Cuts at: 0 793 2181
Size: 793 1388
PflMI CCAn_nnn'nTGG
Cuts at: 0 937 2181
Size: 937 1244
PpuMI rG'GwC_Cy
Cuts at: 0 871 2181
Size: 871 1310
PsiI TTA'TAA
Cuts at: 0 1757 2181
Size: 1757 424
PstI C_TGCA'G
Cuts at: 0 368 1233 2124 2181
Size: 368 865 891 57
Fragments arranged by size:
891 865 368 57
PvuI CG_AT'CG
Cuts at: 0 1052 2181
Size: 1052 1129
PvuII CAG'CTG
Cuts at: 0 2058 2181
Size: 2058 123
SfcI C'TryA_G
Cuts at: 0 364 1229 1292 2120 2181
Size: 364 865 63 828 61
Fragments arranged by size:
865 828 364 63 61
SmlI C'TyrA_G
Cuts at: 0 757 2181
Size: 757 1424
StuI AGG'CCT
Cuts at: 0 1513 2181
Size: 1513 668
StyI C'CwwG_G
Cuts at: 0 345 2181
Size: 345 1836
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1310 2181
Size: 1310 871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 494 934 2181
Size: 381 113 440 1247
Fragments arranged by size:
1247 440 381 113
XmnI GAAnn'nnTTC
Cuts at: 0 419 2181
Size: 419 1762
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApoI BanII
BbsI BbvCI BglI BlpI BmgBI BmrI BpmI Bpu10I
BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstXI BtgI
BtsI CspCI DraI EaeI EarI Eco57MI EcoO109I EcoRV
FalI FspI Hin4I HincII HpaI MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PpuMI
PsiI PstI PvuI PvuII SfcI SmlI StuI StyI
TaqII XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglII Bme1580I BmtI BplI BsaI BsaAI BsaHI BsaWI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspAI
HaeII HindIII KasI KpnI MluI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI
ZraI