Restriction map for
pF1KB4370
(Linear) (Six-base) MAPSORT of: KIBB4370.seq Check: 2062 from: 1 to: 5355
KIBB4370.orf
With 182 enzymes: SgfI *
July 1, 2016 17:01 ..
AatII G_ACGT'C
Cuts at: 0 1849 5355
Size: 1849 3506
AccI GT'mk_AC
Cuts at: 0 3235 4611 5355
Size: 3235 1376 744
Fragments arranged by size:
3235 1376 744
Acc65I G'GTAC_C
Cuts at: 0 5328 5355
Size: 5328 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1322 2765 3094 4412 5355
Size: 1322 1443 329 1318 943
Fragments arranged by size:
1443 1322 1318 943 329
AflIII A'CryG_T
Cuts at: 0 493 4449 4814 5355
Size: 493 3956 365 541
Fragments arranged by size:
3956 541 493 365
AgeI A'CCGG_T
Cuts at: 0 3019 5355
Size: 3019 2336
AhdI GACnn_n'nnGTC
Cuts at: 0 1032 4057 4527 5355
Size: 1032 3025 470 828
Fragments arranged by size:
3025 1032 828 470
AleI CACnn'nnGTG
Cuts at: 0 2746 5349 5355
Size: 2746 2603 6
Fragments arranged by size:
2746 2603 6
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1133 4760 5355
Size: 1133 3627 595
Fragments arranged by size:
3627 1133 595
AlwNI CAG_nnn'CTG
Cuts at: 0 104 567 841 937 1345 1506 3700 3971
Size: 104 463 274 96 408 161 2194 271
Cuts at: 3971 4119 4632 5355
Size: 148 513 723
Fragments arranged by size:
2194 723 513 463 408 274 271 161
148 104 96
ApaI G_GGCC'C
Cuts at: 0 42 385 5355
Size: 42 343 4970
Fragments arranged by size:
4970 343 42
ApoI r'AATT_y
Cuts at: 0 458 2161 2958 4381 5100 5355
Size: 458 1703 797 1423 719 255
Fragments arranged by size:
1703 1423 797 719 458 255
AseI AT'TA_AT
Cuts at: 0 2233 4019 5355
Size: 2233 1786 1336
Fragments arranged by size:
2233 1786 1336
AvaI C'yCGr_G
Cuts at: 0 35 1869 3914 5355
Size: 35 1834 2045 1441
Fragments arranged by size:
2045 1834 1441 35
BamHI G'GATC_C
Cuts at: 0 1982 3377 4991 5355
Size: 1982 1395 1614 364
Fragments arranged by size:
1982 1614 1395 364
BanI G'GyrC_C
Cuts at: 0 5328 5355
Size: 5328 27
BanII G_rGCy'C
Cuts at: 0 42 327 385 855 1361 1670 3002 5252
Size: 42 285 58 470 506 309 1332 2250
Cuts at: 5252 5355
Size: 103
Fragments arranged by size:
2250 1332 506 470 309 285 103 58
42
BbsI GAAGACnn'nnnn_
Cuts at: 0 2033 3034 4811 5355
Size: 2033 1001 1777 544
Fragments arranged by size:
2033 1777 1001 544
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2777 2811 5355
Size: 2777 34 2544
Fragments arranged by size:
2777 2544 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 687 5300 5355
Size: 687 4613 55
Fragments arranged by size:
4613 687 55
BclI T'GATC_A
Cuts at: 0 3702 5355
Size: 3702 1653
BfrBI ATG'CAT
Cuts at: 0 2984 5355
Size: 2984 2371
BglI GCCn_nnn'nGGC
Cuts at: 0 3077 5355
Size: 3077 2278
BglII A'GATC_T
Cuts at: 0 2424 2972 5355
Size: 2424 548 2383
Fragments arranged by size:
2424 2383 548
BlpI GC'TnA_GC
Cuts at: 0 1669 3005 5355
Size: 1669 1336 2350
Fragments arranged by size:
2350 1669 1336
Bme1580I G_kGCm'C
Cuts at: 0 42 296 385 5355
Size: 42 254 89 4970
Fragments arranged by size:
4970 254 89 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 642 1524 3239 4236 5355
Size: 642 882 1715 997 1119
Fragments arranged by size:
1715 1119 997 882 642
BmtI G_CTAG'C
Cuts at: 0 223 5355
Size: 223 5132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2460 3207 5355
Size: 2460 747 2148
Fragments arranged by size:
2460 2148 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 979 1163 1711 1978 2228 2710 2856
Size: 64 915 184 548 267 250 482 146
Cuts at: 2856 3016 5355
Size: 160 2339
Fragments arranged by size:
2339 915 548 482 267 250 184 160
146 64
Bpu10I CC'TnA_GC
Cuts at: 0 1622 1901 3657 4163 5246 5355
Size: 1622 279 1756 506 1083 109
Fragments arranged by size:
1756 1622 1083 506 279 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1696 2430 3526 5355
Size: 1696 734 1096 1829
Fragments arranged by size:
1829 1696 1096 734
BsaI GGTCTCn'nnnn_
Cuts at: 0 3178 3398 3590 4461 5355
Size: 3178 220 192 871 894
Fragments arranged by size:
3178 894 871 220 192
BsaAI yAC'GTr
Cuts at: 0 2133 5355
Size: 2133 3222
BsaBI GATnn'nnATC
Cuts at: 0 1282 4514 4550 5355
Size: 1282 3232 36 805
Fragments arranged by size:
3232 1282 805 36
BsaHI Gr'CG_yC
Cuts at: 0 1331 1846 2695 5355
Size: 1331 515 849 2660
Fragments arranged by size:
2660 1331 849 515
BsaWI w'CCGG_w
Cuts at: 0 3019 5355
Size: 3019 2336
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2107 2137 5355
Size: 2107 30 3218
Fragments arranged by size:
3218 2107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1222 2068 4636 5355
Size: 1222 846 2568 719
Fragments arranged by size:
2568 1222 846 719
BseYI C'CCAG_C
Cuts at: 0 80 89 1644 2010 4751 4823 5355
Size: 80 9 1555 366 2741 72 532
Fragments arranged by size:
2741 1555 532 366 80 72 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1541 2029 5355
Size: 149 1392 488 3326
Fragments arranged by size:
3326 1392 488 149
BsiHKAI G_wGCw'C
Cuts at: 0 1361 1484 1670 3002 4739 5252 5355
Size: 1361 123 186 1332 1737 513 103
Fragments arranged by size:
1737 1361 1332 513 186 123 103
BsmI GAATG_Cn'
Cuts at: 0 2269 2813 5355
Size: 2269 544 2542
Fragments arranged by size:
2542 2269 544
BsmBI CGTCTCn'nnnn_
Cuts at: 0 797 1338 3906 4780 5355
Size: 797 541 2568 874 575
Fragments arranged by size:
2568 874 797 575 541
Bsp1286I G_dGCh'C
Cuts at: 0 42 296 327 385 855 1361 1484 1670
Size: 42 254 31 58 470 506 123 186
Cuts at: 1670 3002 4739 5252 5355
Size: 1332 1737 513 103
Fragments arranged by size:
1737 1332 513 506 470 254 186 123
103 58 42 31
BspHI T'CATG_A
Cuts at: 0 462 3705 4073 5355
Size: 462 3243 368 1282
Fragments arranged by size:
3243 1282 462 368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4210 4417 4912 5355
Size: 4210 207 495 443
Fragments arranged by size:
4210 495 443 207
BsrBI CCG'CTC
Cuts at: 0 1136 3656 5355
Size: 1136 2520 1699
Fragments arranged by size:
2520 1699 1136
BsrDI GCAATG_nn'
Cuts at: 0 2489 3617 5355
Size: 2489 1128 1738
Fragments arranged by size:
2489 1738 1128
BsrFI r'CCGG_y
Cuts at: 0 3019 5355
Size: 3019 2336
BsrGI T'GTAC_A
Cuts at: 0 2564 5355
Size: 2564 2791
BssSI C'ACGA_G
Cuts at: 0 1273 1699 3855 5355
Size: 1273 426 2156 1500
Fragments arranged by size:
2156 1500 1273 426
BstAPI GCAn_nnn'nTGC
Cuts at: 0 104 2007 4219 5355
Size: 104 1903 2212 1136
Fragments arranged by size:
2212 1903 1136 104
BstBI TT'CG_AA
Cuts at: 0 4001 5001 5355
Size: 4001 1000 354
Fragments arranged by size:
4001 1000 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 2658 4974 5355
Size: 621 2037 2316 381
Fragments arranged by size:
2316 2037 621 381
BstYI r'GATC_y
Cuts at: 0 50 1514 1982 2424 2972 3046 3377 4515
Size: 50 1464 468 442 548 74 331 1138
Cuts at: 4515 4991 5355
Size: 476 364
Fragments arranged by size:
1464 1138 548 476 468 442 364 331
74 50
Bsu36I CC'TnA_GG
Cuts at: 0 1675 2357 5355
Size: 1675 682 2998
Fragments arranged by size:
2998 1675 682
BtgI C'CryG_G
Cuts at: 0 1850 3293 5355
Size: 1850 1443 2062
Fragments arranged by size:
2062 1850 1443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 267 5355
Size: 267 5088
BtsI GCAGTG_nn'
Cuts at: 0 158 443 539 2300 4303 5076 5355
Size: 158 285 96 1761 2003 773 279
Fragments arranged by size:
2003 1761 773 285 279 158 96
ClaI AT'CG_AT
Cuts at: 0 3791 5355
Size: 3791 1564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4279 4314 4436 4471 5355
Size: 4279 35 122 35 884
Fragments arranged by size:
4279 884 122 35 35
DraI TTT'AAA
Cuts at: 0 1398 1571 5355
Size: 1398 173 3784
Fragments arranged by size:
3784 1398 173
EaeI y'GGCC_r
Cuts at: 0 512 2671 3158 3644 4086 5355
Size: 512 2159 487 486 442 1269
Fragments arranged by size:
2159 1269 512 487 486 442
EarI CTCTTCn'nnn_
Cuts at: 0 431 1341 1388 2429 2453 3092 4072 4837
Size: 431 910 47 1041 24 639 980 765
Cuts at: 4837 5309 5355
Size: 472 46
Fragments arranged by size:
1041 980 910 765 639 472 431 47
46 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2188 3152 5355
Size: 2188 964 2203
Fragments arranged by size:
2203 2188 964
EcoICRI GAG'CTC
Cuts at: 0 1359 1668 3000 5250 5355
Size: 1359 309 1332 2250 105
Fragments arranged by size:
2250 1359 1332 309 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 979 1163 1322 1711 1978 2228 2710
Size: 64 915 184 159 389 267 250 482
Cuts at: 2710 2765 2856 3016 3094 4412 5355
Size: 55 91 160 78 1318 943
Fragments arranged by size:
1318 943 915 482 389 267 250 184
160 159 91 78 64 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1774 2047 4560 5355
Size: 1774 273 2513 795
Fragments arranged by size:
2513 1774 795 273
EcoO109I rG'GnC_Cy
Cuts at: 0 39 101 381 382 1174 1874 3554 4116
Size: 39 62 280 1 792 700 1680 562
Cuts at: 4116 5355
Size: 1239
Fragments arranged by size:
1680 1239 792 700 562 280 62 39
1
EcoRI G'AATT_C
Cuts at: 0 2958 5100 5355
Size: 2958 2142 255
Fragments arranged by size:
2958 2142 255
EcoRV GAT'ATC
Cuts at: 0 3356 4776 5355
Size: 3356 1420 579
Fragments arranged by size:
3356 1420 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 891 1137 3690 4288 5355
Size: 198 693 246 2553 598 1067
Fragments arranged by size:
2553 1067 693 598 246 198
FspI TGC'GCA
Cuts at: 0 97 5355
Size: 97 5258
HaeII r_GCGC'y
Cuts at: 0 118 1596 2061 5355
Size: 118 1478 465 3294
Fragments arranged by size:
3294 1478 465 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 985 1017 1018 1050 1174 1206 2768 2800
Size: 985 32 1 32 124 32 1562 32
Cuts at: 2800 3402 3434 3959 3991 4043 4075 4156 4188
Size: 602 32 525 32 52 32 81 32
Cuts at: 4188 4513 4545 4779 4811 5355
Size: 325 32 234 32 544
Fragments arranged by size:
1562 985 602 544 525 325 234 124
81 52 32 32 32 32 32 32
32 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1554 3264 3474 5355
Size: 1554 1710 210 1881
Fragments arranged by size:
1881 1710 1554 210
HindIII A'AGCT_T
Cuts at: 0 4253 5355
Size: 4253 1102
KpnI G_GTAC'C
Cuts at: 0 5332 5355
Size: 5332 23
MfeI C'AATT_G
Cuts at: 0 4291 5355
Size: 4291 1064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3405 3643 4403 4649 4823 5355
Size: 3405 238 760 246 174 532
Fragments arranged by size:
3405 760 532 246 238 174
MscI TGG'CCA
Cuts at: 0 514 3160 4088 5355
Size: 514 2646 928 1267
Fragments arranged by size:
2646 1267 928 514
MslI CAynn'nnrTG
Cuts at: 0 28 586 2746 2884 2939 3859 4060 4508
Size: 28 558 2160 138 55 920 201 448
Cuts at: 4508 5095 5349 5355
Size: 587 254 6
Fragments arranged by size:
2160 920 587 558 448 254 201 138
55 28 6
MspA1I CmG'CkG
Cuts at: 0 93 913 1227 2553 2814 2994 3009 3275
Size: 93 820 314 1326 261 180 15 266
Cuts at: 3275 3614 4601 4902 5355
Size: 339 987 301 453
Fragments arranged by size:
1326 987 820 453 339 314 301 266
261 180 93 15
NcoI C'CATG_G
Cuts at: 0 1850 3293 5355
Size: 1850 1443 2062
Fragments arranged by size:
2062 1850 1443
NheI G'CTAG_C
Cuts at: 0 219 5355
Size: 219 5136
NsiI A_TGCA'T
Cuts at: 0 2986 5355
Size: 2986 2369
NspI r_CATG'y
Cuts at: 0 27 497 766 2010 3330 3409 4453 4806
Size: 27 470 269 1244 1320 79 1044 353
Cuts at: 4806 4818 5194 5355
Size: 12 376 161
Fragments arranged by size:
1320 1244 1044 470 376 353 269 161
79 27 12
PasI CC'CwG_GG
Cuts at: 0 328 5355
Size: 328 5027
PciI A'CATG_T
Cuts at: 0 493 4449 4814 5355
Size: 493 3956 365 541
Fragments arranged by size:
3956 541 493 365
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 3087 5355
Size: 861 2226 2268
Fragments arranged by size:
2268 2226 861
PfoI T'CCnGG_A
Cuts at: 0 1994 3596 5355
Size: 1994 1602 1759
Fragments arranged by size:
1994 1759 1602
PpuMI rG'GwC_Cy
Cuts at: 0 1874 4116 5355
Size: 1874 2242 1239
Fragments arranged by size:
2242 1874 1239
PspOMI G'GGCC_C
Cuts at: 0 38 381 5355
Size: 38 343 4974
Fragments arranged by size:
4974 343 38
PstI C_TGCA'G
Cuts at: 0 438 482 672 1112 1226 1664 3786 5355
Size: 438 44 190 440 114 438 2122 1569
Fragments arranged by size:
2122 1569 440 438 438 190 114 44
PvuII CAG'CTG
Cuts at: 0 93 1227 2814 2994 3009 4601 5355
Size: 93 1134 1587 180 15 1592 754
Fragments arranged by size:
1592 1587 1134 754 180 93 15
SacI G_AGCT'C
Cuts at: 0 1361 1670 3002 5252 5355
Size: 1361 309 1332 2250 103
Fragments arranged by size:
2250 1361 1332 309 103
SanDI GG'GwC_CC
Cuts at: 0 1874 5355
Size: 1874 3481
SapI GCTCTTCn'nnn_
Cuts at: 0 431 4072 5309 5355
Size: 431 3641 1237 46
Fragments arranged by size:
3641 1237 431 46
SfcI C'TryA_G
Cuts at: 0 434 478 668 1108 1222 1660 2339 2712
Size: 434 44 190 440 114 438 679 373
Cuts at: 2712 3385 3782 4472 5355
Size: 673 397 690 883
Fragments arranged by size:
883 690 679 673 440 438 434 397
373 190 114 44
SmaI CCC'GGG
Cuts at: 0 37 5355
Size: 37 5318
SmlI C'TyrA_G
Cuts at: 0 1711 2445 3505 5355
Size: 1711 734 1060 1850
Fragments arranged by size:
1850 1711 1060 734
SphI G_CATG'C
Cuts at: 0 2010 5355
Size: 2010 3345
StuI AGG'CCT
Cuts at: 0 1688 1817 3912 4879 5122 5355
Size: 1688 129 2095 967 243 233
Fragments arranged by size:
2095 1688 967 243 233 129
StyI C'CwwG_G
Cuts at: 0 241 545 1164 1208 1850 2271 2952 3111
Size: 241 304 619 44 642 421 681 159
Cuts at: 3111 3293 5355
Size: 182 2062
Fragments arranged by size:
2062 681 642 619 421 304 241 182
159 44
SwaI ATTT'AAAT
Cuts at: 0 1571 5355
Size: 1571 3784
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 825 1762 2086 2243 5355
Size: 825 937 324 157 3112
Fragments arranged by size:
3112 937 825 324 157
TatI w'GTAC_w
Cuts at: 0 2564 4811 5201 5355
Size: 2564 2247 390 154
Fragments arranged by size:
2564 2247 390 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1067 1099 3008 3040 5355
Size: 1067 32 1909 32 2315
Fragments arranged by size:
2315 1909 1067 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2665 3268 3967 5355
Size: 2665 603 699 1388
Fragments arranged by size:
2665 1388 699 603
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2607 3175 3300 3600 5355
Size: 2607 568 125 300 1755
Fragments arranged by size:
2607 1755 568 300 125
XmaI C'CCGG_G
Cuts at: 0 35 5355
Size: 35 5320
XmnI GAAnn'nnTTC
Cuts at: 0 401 3869 4268 5272 5355
Size: 401 3468 399 1004 83
Fragments arranged by size:
3468 1004 401 399 83
ZraI GAC'GTC
Cuts at: 0 1847 5355
Size: 1847 3508
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NheI NsiI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SfcI SmaI SmlI SphI StuI
StyI SwaI TaqII TatI TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AloI ApaLI AscI AsiSI
AvrII BaeI BbeI BbvCI BmgBI BsiEI BsiWI BspEI
BssHII BstEII BstZ17I DraIII DrdI EagI FseI FspAI
HpaI KasI MluI NaeI NarI NdeI NgoMIV NotI
NruI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
XbaI XhoI