Restriction map for
pF1KB4366
(Linear) (Six-base) MAPSORT of: KIBB4366.seq Check: 4866 from: 1 to: 2769
KIBB4366.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1659 1781 2769
Size: 1659 122 988
Fragments arranged by size:
1659 988 122
Acc65I G'GTAC_C
Cuts at: 0 1885 2769
Size: 1885 884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 2576 2769
Size: 185 2391 193
Fragments arranged by size:
2391 193 185
AflIII A'CryG_T
Cuts at: 0 1651 2769
Size: 1651 1118
AleI CACnn'nnGTG
Cuts at: 0 1765 2769
Size: 1765 1004
ApaLI G'TGCA_C
Cuts at: 0 1974 2769
Size: 1974 795
ApoI r'AATT_y
Cuts at: 0 384 1169 1195 2769
Size: 384 785 26 1574
Fragments arranged by size:
1574 785 384 26
AseI AT'TA_AT
Cuts at: 0 436 2769
Size: 436 2333
AvaI C'yCGr_G
Cuts at: 0 221 2043 2769
Size: 221 1822 726
Fragments arranged by size:
1822 726 221
BanI G'GyrC_C
Cuts at: 0 1885 2769
Size: 1885 884
BanII G_rGCy'C
Cuts at: 0 1292 2769
Size: 1292 1477
BbsI GAAGACnn'nnnn_
Cuts at: 0 2636 2769
Size: 2636 133
BciVI GTATCCnnnnn_n'
Cuts at: 0 2288 2451 2769
Size: 2288 163 318
Fragments arranged by size:
2288 318 163
BclI T'GATC_A
Cuts at: 0 1473 2769
Size: 1473 1296
BglII A'GATC_T
Cuts at: 0 1821 2769
Size: 1821 948
Bme1580I G_kGCm'C
Cuts at: 0 1978 2322 2769
Size: 1978 344 447
Fragments arranged by size:
1978 447 344
BmgBI CAC'GTC
Cuts at: 0 852 2769
Size: 852 1917
BmrI ACTGGGnnnn_n'
Cuts at: 0 2076 2297 2769
Size: 2076 221 472
Fragments arranged by size:
2076 472 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1158 1262 1421 2614 2758 2769
Size: 628 530 104 159 1193 144 11
Fragments arranged by size:
1193 628 530 159 144 104 11
Bpu10I CC'TnA_GC
Cuts at: 0 1658 2769
Size: 1658 1111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 183 2769
Size: 100 83 2586
Fragments arranged by size:
2586 100 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 2660 2769
Size: 2660 109
BsaBI GATnn'nnATC
Cuts at: 0 1439 1445 1748 2769
Size: 1439 6 303 1021
Fragments arranged by size:
1439 1021 303 6
BsaHI Gr'CG_yC
Cuts at: 0 150 2769
Size: 150 2619
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2511 2541 2769
Size: 2511 30 228
Fragments arranged by size:
2511 228 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 733 1418 2656 2659 2769
Size: 733 685 1238 3 110
Fragments arranged by size:
1238 733 685 110 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1760 2769
Size: 1222 538 1009
Fragments arranged by size:
1222 1009 538
BsiEI CG_ry'CG
Cuts at: 0 525 668 2107 2769
Size: 525 143 1439 662
Fragments arranged by size:
1439 662 525 143
BsiHKAI G_wGCw'C
Cuts at: 0 1659 1978 2769
Size: 1659 319 791
Fragments arranged by size:
1659 791 319
BsmI GAATG_Cn'
Cuts at: 0 1213 2769
Size: 1213 1556
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2487 2769
Size: 2487 282
Bsp1286I G_dGCh'C
Cuts at: 0 1292 1659 1978 2322 2769
Size: 1292 367 319 344 447
Fragments arranged by size:
1292 447 367 344 319
BspHI T'CATG_A
Cuts at: 0 1347 1437 1908 2769
Size: 1347 90 471 861
Fragments arranged by size:
1347 861 471 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1659 1781 2769
Size: 1659 122 988
Fragments arranged by size:
1659 988 122
BsrBI CCG'CTC
Cuts at: 0 1142 2769
Size: 1142 1627
BsrDI GCAATG_nn'
Cuts at: 0 686 2769
Size: 686 2083
BsrFI r'CCGG_y
Cuts at: 0 13 2769
Size: 13 2756
BssSI C'ACGA_G
Cuts at: 0 86 2769
Size: 86 2683
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2592 2736 2769
Size: 2592 144 33
Fragments arranged by size:
2592 144 33
BstYI r'GATC_y
Cuts at: 0 1821 1903 2182 2769
Size: 1821 82 279 587
Fragments arranged by size:
1821 587 279 82
BtsI GCAGTG_nn'
Cuts at: 0 883 1210 1362 2769
Size: 883 327 152 1407
Fragments arranged by size:
1407 883 327 152
ClaI AT'CG_AT
Cuts at: 0 2103 2769
Size: 2103 666
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 240 275 2769
Size: 240 35 2494
Fragments arranged by size:
2494 240 35
EaeI y'GGCC_r
Cuts at: 0 526 1769 2173 2769
Size: 526 1243 404 596
Fragments arranged by size:
1243 596 526 404
EarI CTCTTCn'nnn_
Cuts at: 0 2490 2552 2648 2684 2769
Size: 2490 62 96 36 85
Fragments arranged by size:
2490 96 85 62 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 628 1158 1262 1421 2576 2614 2758
Size: 185 443 530 104 159 1155 38 144
Cuts at: 2758 2769
Size: 11
Fragments arranged by size:
1155 530 443 185 159 144 104 38
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1301 2737 2769
Size: 1301 1436 32
Fragments arranged by size:
1436 1301 32
EcoO109I rG'GnC_Cy
Cuts at: 0 1306 2769
Size: 1306 1463
EcoRV GAT'ATC
Cuts at: 0 2199 2375 2769
Size: 2199 176 394
Fragments arranged by size:
2199 394 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 334 2749 2769
Size: 334 2415 20
Fragments arranged by size:
2415 334 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 1641 1673 2769
Size: 55 32 1554 32 1096
Fragments arranged by size:
1554 1096 55 32 32
HincII GTy'rAC
Cuts at: 0 558 2769
Size: 558 2211
KpnI G_GTAC'C
Cuts at: 0 1889 2769
Size: 1889 880
MfeI C'AATT_G
Cuts at: 0 299 2769
Size: 299 2470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 2769
Size: 688 2081
MscI TGG'CCA
Cuts at: 0 1771 2175 2769
Size: 1771 404 594
Fragments arranged by size:
1771 594 404
MslI CAynn'nnrTG
Cuts at: 0 287 473 965 1601 1754 1765 2769
Size: 287 186 492 636 153 11 1004
Fragments arranged by size:
1004 636 492 287 186 153 11
MspA1I CmG'CkG
Cuts at: 0 358 2554 2663 2769
Size: 358 2196 109 106
Fragments arranged by size:
2196 358 109 106
NaeI GCC'GGC
Cuts at: 0 15 2769
Size: 15 2754
NdeI CA'TA_TG
Cuts at: 0 618 992 2769
Size: 618 374 1777
Fragments arranged by size:
1777 618 374
NgoMIV G'CCGG_C
Cuts at: 0 13 2769
Size: 13 2756
NspI r_CATG'y
Cuts at: 0 1109 1655 2769
Size: 1109 546 1114
Fragments arranged by size:
1114 1109 546
PciI A'CATG_T
Cuts at: 0 1651 2769
Size: 1651 1118
PpuMI rG'GwC_Cy
Cuts at: 0 1306 2769
Size: 1306 1463
PspXI vC'TCGA_Gb
Cuts at: 0 221 2769
Size: 221 2548
PstI C_TGCA'G
Cuts at: 0 462 890 2769
Size: 462 428 1879
Fragments arranged by size:
1879 462 428
PvuI CG_AT'CG
Cuts at: 0 2107 2769
Size: 2107 662
PvuII CAG'CTG
Cuts at: 0 358 2554 2769
Size: 358 2196 215
Fragments arranged by size:
2196 358 215
SapI GCTCTTCn'nnn_
Cuts at: 0 2684 2769
Size: 2684 85
ScaI AGT'ACT
Cuts at: 0 48 704 2769
Size: 48 656 2065
Fragments arranged by size:
2065 656 48
SfcI C'TryA_G
Cuts at: 0 458 639 886 1283 1496 1898 2450 2769
Size: 458 181 247 397 213 402 552 319
Fragments arranged by size:
552 458 402 397 319 247 213 181
SmlI C'TyrA_G
Cuts at: 0 79 198 221 2769
Size: 79 119 23 2548
Fragments arranged by size:
2548 119 79 23
SspI AAT'ATT
Cuts at: 0 2060 2769
Size: 2060 709
StuI AGG'CCT
Cuts at: 0 837 2769
Size: 837 1932
StyI C'CwwG_G
Cuts at: 0 2670 2769
Size: 2670 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 444 1094 2769
Size: 444 650 1675
Fragments arranged by size:
1675 650 444
TatI w'GTAC_w
Cuts at: 0 46 702 1587 2063 2312 2769
Size: 46 656 885 476 249 457
Fragments arranged by size:
885 656 476 457 249 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2511 2543 2769
Size: 2511 32 226
Fragments arranged by size:
2511 226 32
XhoI C'TCGA_G
Cuts at: 0 221 2769
Size: 221 2548
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI ApaLI ApoI AseI
AvaI BanI BanII BbsI BciVI BclI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI
BtsI ClaI CspCI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRV FalI Hin4I HincII KpnI MfeI MmeI MscI
MslI MspA1I NaeI NdeI NgoMIV NspI PciI PpuMI
PspXI PstI PvuI PvuII SapI ScaI SfcI SmlI
SspI StuI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BfrBI BglI BlpI BmtI BplI
BsaAI BsaWI BseYI BsiWI BspEI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI FseI FspI FspAI
HaeII HindIII HpaI KasI MluI NarI NcoI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XcmI XmaI XmnI ZraI