(Linear) (Six-base) MAPSORT of: KIBB4244.seq  Check: 3657  from: 1  to: 819



KIBB4244.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AatII G_ACGT'C

Cuts at:      0     119     819 
   Size:        119     700
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      95     296     431     819 
   Size:         25      70     201     135     388

  Fragments arranged by size: 

                 388     201     135      70      25
 
ApoI r'AATT_y

Cuts at:      0     415     425     819 
   Size:        415      10     394

  Fragments arranged by size: 

                 415     394      10
 
AseI AT'TA_AT

Cuts at:      0     758     819 
   Size:        758      61
 
AvaI C'yCGr_G

Cuts at:      0     175     819 
   Size:        175     644
 
BanII G_rGCy'C

Cuts at:      0     388     819 
   Size:        388     431
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      13     819 
   Size:         13     806
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     651     819 
   Size:        651     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615     644     819 
   Size:        615      29     175

  Fragments arranged by size: 

                 615     175      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     819 
   Size:        303     516
 
BsaHI Gr'CG_yC

Cuts at:      0     116     819 
   Size:        116     703
 
Bsp1286I G_dGCh'C

Cuts at:      0     388     819 
   Size:        388     431
 
BsrDI GCAATG_nn'

Cuts at:      0     655     819 
   Size:        655     164
 
BstYI r'GATC_y

Cuts at:      0      80     363     436     819 
   Size:         80     283      73     383

  Fragments arranged by size: 

                 383     283      80      73
 
Bsu36I CC'TnA_GG

Cuts at:      0     577     819 
   Size:        577     242
 
BtgI C'CryG_G

Cuts at:      0     650     819 
   Size:        650     169
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     725     819 
   Size:        725      94
 
EarI CTCTTCn'nnn_

Cuts at:      0     709     758     819 
   Size:        709      49      61

  Fragments arranged by size: 

                 709      61      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      95     296     431     615     644     819 
   Size:         25      70     201     135     184      29     175

  Fragments arranged by size: 

                 201     184     175     135      70      29      25
 
EcoRI G'AATT_C

Cuts at:      0     415     819 
   Size:        415     404
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     518     550     819 
   Size:        518      32     269

  Fragments arranged by size: 

                 518     269      32
 
MfeI C'AATT_G

Cuts at:      0     226     819 
   Size:        226     593
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     819 
   Size:        184     635
 
MspA1I CmG'CkG

Cuts at:      0      45     819 
   Size:         45     774
 
NcoI C'CATG_G

Cuts at:      0     650     819 
   Size:        650     169
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     383     415     819 
   Size:        383      32     404

  Fragments arranged by size: 

                 404     383      32
 
PsiI TTA'TAA

Cuts at:      0     679     819 
   Size:        679     140
 
PstI C_TGCA'G

Cuts at:      0      88     819 
   Size:         88     731
 
PvuII CAG'CTG

Cuts at:      0      45     819 
   Size:         45     774
 
SfcI C'TryA_G

Cuts at:      0      84     819 
   Size:         84     735
 
SmaI CCC'GGG

Cuts at:      0     177     819 
   Size:        177     642
 
SmlI C'TyrA_G

Cuts at:      0     282     819 
   Size:        282     537
 
SpeI A'CTAG_T

Cuts at:      0     478     819 
   Size:        478     341
 
SspI AAT'ATT

Cuts at:      0     270     819 
   Size:        270     549
 
StyI C'CwwG_G

Cuts at:      0     650     819 
   Size:        650     169
 
TatI w'GTAC_w

Cuts at:      0     222     819 
   Size:        222     597
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     200     819 
   Size:        200     619
 
XmaI C'CCGG_G

Cuts at:      0     175     819 
   Size:        175     644
 
ZraI GAC'GTC

Cuts at:      0     117     819 
   Size:        117     702
 

 Enzymes that do cut:

    AatII     AcuI     ApoI     AseI     AvaI    BanII     BbsI     BmrI
     BpmI    BpuEI    BsaHI Bsp1286I    BsrDI    BstYI   Bsu36I     BtgI
     DrdI     EarI  Eco57MI    EcoRI    Hin4I     MfeI     MmeI   MspA1I
     NcoI     PpiI     PsiI     PstI    PvuII     SfcI     SmaI     SmlI
     SpeI     SspI     StyI     TatI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SphI     SrfI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmnI